1.Safety of simultaneous vaccination of 13-valent pneumococcal polysaccharide conjugate vaccine with other vaccines in Shanghai from 2017 to 2021
Shoufei YANG ; Jiechen LIU ; Zhuoying HUANG ; Qingrui BAI ; Xiang GUO ; Jia REN
Shanghai Journal of Preventive Medicine 2022;34(8):751-755
ObjectiveTo compare the incidence of adverse events following immunization (AEFI) between vaccination with 13-valent pneumococcal polysaccharide conjugate vaccine (PCV13) alone and with other vaccines at the same time. MethodsWe obtained PCV13 vaccination adverse reaction reports for children vaccinated in Shanghai between 2017‒2021 from the National Immunization Program Information Management System and collected the information and the data of children vaccinated with PCV13 from The Shanghai vaccination information system. We compared the incidence of adverse reactions between stand-alone and simultaneous vaccination. ResultsA total of 11 175 PCV13 AEFI cases after stand-alone vaccination and 215 PCV13 AEFI cases after simultaneous vaccination were reported during 2017‒2021 in Shanghai, and the incidence rates were 1 252.34/105 and 1 455.75/105, respectively. ORV was the one mostly vaccinated with PCV13 at the same time, and the AEFI incidence was 1 706.67/105. The most reported symptom was fever after PCV13 vaccination, no matter by stand-alone and simultaneous vaccination with the respective incidence rates of 876.69/105 and 1 442.21/105. ConclusionIn general, PCV13 vaccinated with other vaccines at the same time slightly increases the incidence of AEFI.
2. Observation on safety of sequential vaccination schedule of different strain inactivated poliovirus vaccines
Zhuoying HUANG ; Xiaodong SUN ; Jiechen LIU ; Zhi LI ; Jia REN ; Linlin WU ; Jiayu HU ; Junnan ZHANG
Chinese Journal of Epidemiology 2019;40(5):565-570
Objective:
To evaluate the safety of population based sequential vaccination schedule of inactivated poliovirus vaccines prepared with different strains.
Methods:
This randomized, parallel-group controlled trial was conducted from March, 2017 to May, 2018, in Shanghai. Adverse reaction data of Sabin strain inactivated polio vaccine (sIPV), wild strains inactivated polio vaccines (wIPV) and bivalent types Ⅰ and Ⅲ oral poliomyelitis vaccine (bOPV) were systematically collected through active observation in 1 917 infants in Shanghai after the vaccination at 2, 3, 4 months old. The eligible infants aged 2 months were divided into 4 groups: ①sIPV+sIPV+bOPV group; ②sIPV+wIPV+bOPV group; ③wIPV+sIPV+bOPV group; ④wIPV+wIPV+bOPV group.
Results:
The incidence of adverse reaction 30 days later after 3 basic dose vaccinations was 16.79% (946/5 633). No serious adverse reaction was reported. Local and systemic reactions were mainly mild. Common local reactions were pain, erythema, cutaneous nodule,
3.Application of StrucGP in medical immunology: site-specific N-glycoproteomic analysis of macrophages.
Pengfei LI ; Zexuan CHEN ; Shanshan YOU ; Yintai XU ; Zhifang HAO ; Didi LIU ; Jiechen SHEN ; Bojing ZHU ; Wei DAN ; Shisheng SUN
Frontiers of Medicine 2023;17(2):304-316
The structure of N-glycans on specific proteins can regulate innate and adaptive immunity via sensing environmental signals. Meanwhile, the structural diversity of N-glycans poses analytical challenges that limit the exploration of specific glycosylation functions. In this work, we used THP-1-derived macrophages as examples to show the vast potential of a N-glycan structural interpretation tool StrucGP in N-glycoproteomic analysis. The intact glycopeptides of macrophages were enriched and analyzed using mass spectrometry (MS)-based glycoproteomic approaches, followed by the large-scale mapping of site-specific glycan structures via StrucGP. Results revealed that bisected GlcNAc, core fucosylated, and sialylated glycans (e.g., HexNAc4Hex5Fuc1Neu5Ac1, N4H5F1S1) were increased in M1 and M2 macrophages, especially in the latter. The findings indicated that these structures may be closely related to macrophage polarization. In addition, a high level of glycosylated PD-L1 was observed in M1 macrophages, and the LacNAc moiety was detected at Asn-192 and Asn-200 of PD-L1, and Asn-200 contained Lewis epitopes. The precision structural interpretation of site-specific glycans and subsequent intervention of target glycoproteins and related glycosyltransferases are of great value for the development of new diagnostic and therapeutic approaches for different diseases.
Humans
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B7-H1 Antigen
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Glycosylation
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Polysaccharides/metabolism*
4. Immunogenicity analysis of sequential inoculation of different strains of poliomyelitis vaccines in Shanghai
Zhuoying HUANG ; Xiaodong SUN ; Minyong LIU ; Chongshan LI ; Jia REN ; Jiayu HU ; Jianping YANG ; Jiechen LIU ; Zhi LI ; Yuying YANG ; Yunyi LI ; Jing LU
Chinese Journal of Preventive Medicine 2019;53(5):513-518
Objective:
To evaluate the immunogenicity of different strains of inactivated poliomyelitis vaccines (IPV) by sequential program.
Methods:
This parallel-group controlled trial was conducted in immunization clinics in Shanghai from March 2016 to September 2017. Sabin strains inactivated poliomyelitis vaccines (sIPV), WPV strains inactivated poliomyelitis vaccines (wIPV) and live poliomyelitis Type Ⅰ Type Ⅲ vaccine (bOPV) as the investigational vaccine were used at 2, 3, 4 months old in 325 infants in Shanghai. Infants vaccinated by four sequential program were divided into 4 groups: sIPV+sIPV+bOPV, sIPV+wIPV+bOPV, wIPV+sIPV+bOPV and wIPV+wIPV+bOPV. A total of 230 investigators′ blood samples were collected before primary immunization and 163 investigators′ blood samples were collected after primary immunization. A total of 151 investigators (36, 44, 30 and 41 in each group) finished primary immunization and blood sampling before and after the primary immunization. The geometric mean titer (GMT) of poliovirus typesⅠ and Ⅲ neutralizing antibody was tested and calculated, and the positive results of antibody before and after primary immunization were analyzed.
Results:
Among the 151 investigators, the age were (2.27±0.61) months and birth weight were (3.27±0.43) kg, and 70 were male. The positive rates of typeⅠwas 98.68% (149 cases), and type Ⅲ was 97.35% (147 cases); the number of investigators tested in each group was 36, 44, 30 and 41, respectively; the positive rates of typeⅠwas 97.22% (35 cases), 100.00% (44 cases), 96.67% (29 cases) and 100.00% (41 cases) (