2.Establishing a 29 Y-STR Loci Multiplex PCR System.
Xin-jie WANG ; Li-jing LUO ; Lei HUANG ; Xin XU
Journal of Forensic Medicine 2015;31(6):456-461
OBJECTIVE:
To establish a 29 Y-STR loci multiplex PCR system for investigating the genetic polymorphisms and to assess its application value in forensic science.
METHODS:
A multiplex PCR system was established using a five color fluorescence labeling 29 Y-STR loci (DYS456, DYS389 I , DYS437, DYS447, DYS389 11, DYS438, DYS522, DYS460, DYS458, DYS622, DYS390, DYS392, DYS448, DYS449, DYS391, Y-GA TA-H4, DYS388, DYS19, DYS385a/b, DYS527a/b, DYS393, DYS459a/b, DYS635, DYS439, DYS570 and DYS627) for multiple amplification and capillary electrophoresis. And its applicability was validated with genetic polymorphism data of 29 Y-STR of unrelated 2,000 male samples in Shandong Han population.
RESULTS:
A total of 1,981 different haplotypes of 2,000 individuals showed genotype diver- sity between 0.370 0 and 0.965 4. The system provided stable and accurate typing with high sensitivity of 0.05 ng. It satisfied the needs of variety of routine biological samples.
CONCLUSION
The 29 Y-STR loci multiplex PCR system could be applied for actual cases and establishment of Y-STR database. In addition, it has great significance in forensic science practices and related research.
Asian People/genetics*
;
China
;
Chromosomes, Human, Y
;
DNA/isolation & purification*
;
Ethnicity/genetics*
;
Forensic Genetics/methods*
;
Forensic Sciences
;
Genetics, Population/methods*
;
Haplotypes
;
Humans
;
Male
;
Microsatellite Repeats
;
Multiplex Polymerase Chain Reaction/methods*
;
Polymorphism, Genetic
;
Reproducibility of Results
3.The expression of platelet activity in hypertensive patients complicated with ischemic cardiovascular and cerebrovascular disease
Xu LIANG ; Lin-Jie LUO ; Xin-Gen WANG ; Al ET ;
Chinese Journal of Practical Internal Medicine 2001;0(04):-
Objective To observe the change of platelet activity marker in hypertensive patients complicated with ischemic cardiovascular and cerebrovascular disease ,and assess it s value in the happening of thrombotic disease. Method The platelet activity marker (CD62p,CD63) were measured by flow cytometry in 40 hypertensive patients complicated with ischemic cardiovascular and cerebrovascular disease (group C ),46 hypertensive patients not complicated with ischemic cardiovascular and cerebrovascular disease (group B),and 30 cases of healthy control (group A). Comparison between three groups were made. The serum fibrinogen were measured at the same time. Result serum level of CD62p, and CD63 in group B,C are higher than group A.(P
4.Angioimmunoblastic T cell lymphoma with Reed-Sternberg-like cells.
Yan-hui LIU ; Heng-guo ZHUANG ; Dong-lan LUO ; Xin-lan LUO ; Jie XU
Chinese Journal of Pathology 2006;35(4):249-250
Aged
;
Antigens, CD20
;
analysis
;
Gene Rearrangement
;
Humans
;
Immunoblastic Lymphadenopathy
;
genetics
;
metabolism
;
pathology
;
Immunoglobulin Heavy Chains
;
genetics
;
Immunohistochemistry
;
Lymph Nodes
;
metabolism
;
pathology
;
Lymphoma, T-Cell
;
genetics
;
metabolism
;
pathology
;
Male
;
Polymerase Chain Reaction
;
RNA, Viral
;
analysis
;
Receptors, Antigen, T-Cell, gamma-delta
;
genetics
;
Reed-Sternberg Cells
;
metabolism
;
pathology
5.Therapeutic effects of blood purification in 160 cases with liver failure
Weijiang YE ; Jie JIN ; Haiyan YU ; Jie WANG ; Xin LUO ; Jinsong HUANG
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2006;0(06):-
Objective: To evaluate the clinical efficacy of various methods of blood purification in the treatment of patients with liver failure.Methods: Two hundred and seventy-three patients suffering from liver failure were randomly divided into two groups.On the base of medical routine treatment,160 patients were additionally treated with various methods of blood purification treatment group,including plasma exchange,plasma exchange associated with continuously venovenous hemofiltration,plasma exchange associated with hemodiafiltration,plasma exchange associated with plasma absorption,molecular absorbents recycling(system) and so on.While the other 113 patients received medical routine treatment only(control group).The efficacy of treatment and prognosis in the two groups were then observed and compared.Results: In the treatment group,the total bilirubin(TB),albumin(ALB),total bile acid(TBA),alanine aminotransferase(ALT),pre-albumin(PALB),cholinesterase(CHE) and prothrombin activity(PTA) after the treatment were better compared with those before the treatment(all P
6.Evaluation on tolerability and safety of long-term administration with shexiang baoxin pill in patients with coronary heart disease of stable angina pectoris.
Li-Jie WANG ; Xin-Ping LUO ; Yong WANG
Chinese Journal of Integrated Traditional and Western Medicine 2008;28(5):399-401
OBJECTIVETo evaluate the safety and tolerability of long-term (6 months) administration with Shexiang Baoxin Pill (SBP) in patients with coronary heart disease (CHD) of stable angina pectoris.
METHODSAdopting randomized non-blinded and parallel controlled trial, 200 patients with CHD were randomly and equally assigned to the SBP group and the control group. Both received basic therapy for CHD, including anti-platelet, lipid regulating and anti-ischemia with additional SBP 2 pills, taken orally three times per day in the SBP group. They were followed up for 6 months. The drug tolerability and adverse drug reactions occurred in the observation period were recorded, and the laboratory indexes involving blood routine, liver function, renal function, blood glucose and blood lipids were detected before and after treatment.
RESULTSThe trial was completed in 92% of the patients, 5 patients withdrew in the SBP group and 11 patients in the control group; but none for the intolerable therapy. There were 1 case of adverse reaction related to SBP. No obvious change was found in blood glucose and blood lipids in the two groups before and after treatment. No serious adverse reaction and injury of liver and renal function or others happened.
CONCLUSIONLong-term administration of SBP has favorable clinical tolerability and safety for CHD patients.
Angina Pectoris ; drug therapy ; Coronary Disease ; drug therapy ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Tablets
7.Effects of pH9.0 Tris-EDTA and pH6.0 citrate on antigen retrieval.
Xin-Lan LUO ; Yan-Hui LIU ; Heng-Guo ZHUANG ; Xiu-Ling CAI ; Jie XU
Chinese Journal of Pathology 2005;34(11):749-751
Antigens
;
analysis
;
Cervical Intraepithelial Neoplasia
;
metabolism
;
Citric Acid
;
Cyclin-Dependent Kinase Inhibitor p21
;
analysis
;
Edetic Acid
;
Formaldehyde
;
Hot Temperature
;
Humans
;
Hydrogen-Ion Concentration
;
Immunohistochemistry
;
Intestinal Mucosa
;
immunology
;
Ki-67 Antigen
;
analysis
;
Microwaves
;
Palatine Tonsil
;
metabolism
;
Proto-Oncogene Proteins c-bcl-6
;
analysis
8.Evaluation on clinical effect of long-term shexiang baoxin pill administration for treatment of coronary heart disease.
Hui ZHU ; Xin-Ping LUO ; Li-Jie WANG
Chinese Journal of Integrated Traditional and Western Medicine 2010;30(5):474-477
OBJECTIVETo evaluate the effect of long-term Shexiang Baoxin Pill (SXBXP) administration on cardiovascular events in patients with stable angina pectoris (SAP).
METHODSA prospective randomized non-blind parallel controlled study was conducted in the early stage (the first 6 months) of the trial, then a cohort study was succeeded in the later stage. Two hundred patients with SAP, who visited the hospital between May 2005 and June 2006, were selected and randomly assigned to the trial group and the control group, 100 patients in each group. Both were treated with conventional therapy, including treatment for anti-platelet, blood lipid regulating, anti-ischemia, etc, and to patients in the trial group, SXBXP was administered additionally for 2 pills, three times a day by oral intake. The therapeutic course lasted for at least 6 months. All patients were followed up until January 2008, the clinical events and conditions of treatment were recorded. The composite terminal of various cardiovascular events was regarded as the primary endpoint.
RESULTSThe median follow-up time of the study was 2.25 years (ranging from 0.5 to 2.75 years). In the trial group, the occurrence (cases) was 23 for all-clinical event, 20 for primary-clinical event and 9 for angina pectoris event, which were lesser than those in the control group, 33, 29 and 19 cases respectively, showing a significant difference between groups (P < 0.05). The dosage of nitrates used in the trial was decreased more than that before treatment. Besides, all the incidences (cases), in terms of all-cause death (2 vs 5), cardiovascular death (1 vs 2), congestive heart failure (3 vs 4), stroke (2 vs 4), and other clinical (5 vs 6) events, as well as in the need for percutaneous coronary intervention or coronary artery bypass graft (2 vs 4), showed somewhat lowering in the trial group as compared with the corresponding items in the control group, but statistical analysis showed an insignificant difference between them (P > 0.05).
CONCLUSIONLong-term SXBXP administration could reduce the occurrence of angina pectoris events and some other clinical events, and cut down the dosage of nitrates used in patients with SAP.
Adult ; Aged ; Aged, 80 and over ; Coronary Disease ; drug therapy ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Phytotherapy ; Prospective Studies ; Treatment Outcome
9.Antigen retrieval for immunohistochemistry performed on destained paraffin sections.
Xin-lan LUO ; Jie XU ; Yan-hui LIU ; Heng-guo ZHUANG
Chinese Journal of Pathology 2007;36(1):59-60
Antigens
;
analysis
;
immunology
;
Antigens, CD
;
analysis
;
immunology
;
Formaldehyde
;
Hot Temperature
;
Humans
;
Immunohistochemistry
;
methods
;
Neoplasms
;
metabolism
;
pathology
;
Paraffin Embedding
;
methods
;
Staining and Labeling
;
Tissue Fixation
;
methods
10.Epstein-Barr virus-positive diffuse large B-cell lymphoma following peripheral T-cell lymphoma, not otherwise specified: report of a case.
Fen ZHANG ; Yan-hui LIU ; Heng-guo ZHUANG ; Li LI ; Xin-lan LUO ; Jie XU
Chinese Journal of Pathology 2010;39(6):414-415
Aged
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
Cyclophosphamide
;
therapeutic use
;
Doxorubicin
;
therapeutic use
;
Epstein-Barr Virus Infections
;
Female
;
Herpesvirus 4, Human
;
Humans
;
Lymphoma, Large B-Cell, Diffuse
;
drug therapy
;
pathology
;
virology
;
Lymphoma, T-Cell, Peripheral
;
drug therapy
;
pathology
;
Neoplasms, Second Primary
;
drug therapy
;
pathology
;
Prednisone
;
therapeutic use
;
Vincristine
;
therapeutic use