1.Heiner syndrome in a case.
Li-feng SHI ; Ping WANG ; Jie LI
Chinese Journal of Pediatrics 2010;48(6):473-474
Humans
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Infant
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Lung Diseases
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etiology
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Male
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Milk Hypersensitivity
2.Awareness rate of child health management in parents of children aged 0 - 36 months
Xiaobing FU ; Jie LIAN ; Ping SHI
Chinese Journal of General Practitioners 2012;(7):519-521
A questionnaire survey on awareness of child health management was conducted in 238 family members ( 236 were parents) of children aged 0 - 36 months; among whom 92 of children aged 0 to 6 months,85 of children aged 7 to 12 months,33 of children aged 13 to 18 months,16 of children aged 19 to 24 months and 12 of children aged 24 to 36 months.52.1% of the surveyees had college education level.The awareness about medical examination in last half year in female subjects was higher than males ( P < 0.05).There were no differences in other health management knowledge.The results showed that the average times of medical examination was 2.8 ± 1.9 in last half year; the age for supplement of meat in food was 7.4 ± 2.4 mouths; supplement of meat,animal liver and eggs was more than 7 time a week.The subjects with college education or above paid more attention to the children's growth,risk for rickets and anemia than other peoples.Pure breast feeding accounted for only 37.0% (88/238).The study indicates that strengthening the knowledge and awareness is necessary to improve the children health management.
5.Multi-slice spiral CT angiography of the posterior nutcracker syndrome
Long ZHAO ; Heshui SHI ; Xi LIU ; Ping HAN ; Jie YU
Chinese Journal of Medical Imaging Technology 2009;25(10):1817-1820
Objective To assess the value of multi-slice spiral CT angiography (MSCTA) in diagnosis of posterior nutcracker syndrome (NCS). Methods MSCTA of 15 patients clinically diagnosed as posterior NCS (patient group) and 50 subjects with normal renal vessels (control group) were retrospectively analyzed. The anatomy, course and relationship to the adjacent structure of left renal vein (LRV) and its branches were observed. The anteroposterior diameter (d1) and cross areas (s1) of the angle of control group and posterior abdominal artery (AA), the anteroposterior diameter (d2) and cross areas (s2) at the largest lumen near the renal hilar were measured and the ratio of s2/s1 and d2/ d1 were calculated.Results All LRVs posterior AA of patient group were compressed and narrowing with dilating apparently near the renal hilar. The difference of d2 was not significant (P>0.05), but of other values were significant (P<0.05).Conclusion MSCTA can show anatomy and three-dimentional trend clearly of LRV for posterior NCS, and evaluate the extent of narrow and dilating lumen accurately with cross areas measuring.
8.Development of excitotoxicity model with primary hippocampal neurons and implications of viability essay
Jie SHEN ; Changkai SUN ; Ming FAN ; Hui MA ; Ping LIN ; Yuying TONG ; Guangxia SHI
Chinese Journal of Laboratory Medicine 2003;0(10):-
Objective To study the injury and protective action of drugs on neurons, the model of glutamate excitotoxicity on primary cultured hippocampual neurons from new born rats were( set up. Methods)By use of trypan blue dye staning and testing the lactate dehydrogenase leakage from cultured neurons, to investigate the neuron survival rate. Results We found the injury of neurons was related with the concentration of glutamate. NMDAR non-competitive antagonist —MK-801 could protect the glutamate excitotoxic damage on neurons. Conclusion The glutamate results in neuron injury through NMDAR; the model of neuron culture was sufficient for glutamate-induced excitotoxicity.
9.Experimental mouse model of Chlamydia pneumoniae strain pneumonitis
Yi SHI ; Jie YIN ; Huawen ZHAN ; Xin SU ; Xirong XIA ; Xiaojun ZHOU ; Ping SHEN
Journal of Medical Postgraduates 2001;14(1):6-8
Objectives: To evaluate mice as experimental animal for Chlamydia pneumoniae, a common cause of acute respiratory infections in human. Methods: Intranasal inoculation of Icr mice with C. Pneumoniae induced a prolonged course of lung infection, as demonstrated by persistence of lung pathology(60 days). Results: Icr mice were susceptible to C. pneumoniae. Lung pathology was characterized by patchy interstitial pneumonitis with predominately neutrophil leukocyte infiltration in the early(7 days) and lymphocytes infiltration in the later stages(14 days later) of infection. Conclusions:Icr mice were susceptible to C. pneumoniae and the mouse model is useful for the investigation of the pathogenesis of C. pneumoniae infection.
10.Determination of skimmin, scopolin and umbelliferone in Tibetan medicine Saussurea hieracioides by HPLC.
Rong TAN ; Yu-Jie WANG ; Yu-Xin ZHANG ; Li-Shi ZHOU ; Er TAN ; A PING ; Yi ZHANG
China Journal of Chinese Materia Medica 2014;39(6):1054-1057
This study is aimed to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of skimmin, scopolin and umbelliferone in Saussurea hieracioides. Samples were analyzed on a Wondasil C18-WR column (4.6 mm x 250 mm, 5 microm) with methanol (A) and water containing 0.1% phosphate (B) as mobile phases for gradient elution at a flow rate of 1.0 mL x min(-1). The detection wavelength and column temperature were set at 325 nm and 35 degrees C, respectively, and the sample size was 10 microL. The results showed that skimmin, scopolin and umbelliferone were simultaneously achieved within 40 min under the above conditions. A good linearity was observed in the range of 0.18-5.6 microg (r = 1.000 0), 0.060-1.8 microg (r = 0.999 9), 0.032-0.97 microg (r = 0.999 8) for skimmin, scopolin and umbelliferone, respectively, with the average recoveries of 99.16% (RSD = 0.41%), 100.3% (RSD = 0.79%), 102.2% (RSD = 0.87%). The method is simple, accurate and reproducible and can be used for the quality control of S. hieracioides.
Chromatography, High Pressure Liquid
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Coumarins
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analysis
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Glucosides
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analysis
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Medicine, Tibetan Traditional
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Reproducibility of Results
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Saussurea
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chemistry
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Umbelliferones
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analysis