Objective To study the changing of subsets of blood lymphocyte in adult SARS patients and its effect on the clinical features and prognosis. Methods According to the clinical characteristic diagnostic standards of SARS recommended by the Ministry of Health of China, 206 of hospita lized SARS patients were divided into 3 groups: Mild-Moderate group included 13 3 patients; severe group 50 patients and death group 23 patients, and cells coun t changes of CD4 +, CD8 +, CD19 + and CD16 +. Statistic analyses were perfor med to analyze the relationship of immune changes and clinical features and prognosis. Results The counts of CD4 +, CD8 +, CD19 + lymphocytes in mild-moderate group were h igher than severe group, while lowest in death group (P0.05), there were significanl y difference in CD4 +, CD8 +,CD19 + and CD16 + cell counts among three grou ps(P

ObjectiveTo observe the clinical efficacy of acupuncture in treating musculospiral paralyses.MethodSixty patients with musculospiral paralyses were elected to receive acupuncture treatment. The motor function and electromyogram (EMG) were detected after 14-day treatment.ResultOf the 60 patients, 36 were recovered, 21 showed improvement, 3 failed in the treatment, and the total effective rate was 95.0%.ConclusionAcupuncture can produce a significant efficacy in treating musculospiral paralyses, and it benefits the recovery of motor function of the impaired nerve.

Study of traditional Chinese medicine (TCM) syndromes is a key to the research of TCM modernization, and the core is the classification and diagnostic criteria of syndromes. The purpose of this article is to review the usage of classification algorithms of data mining in TCM syndrome researches, and comprehensively analyze the main features of algorithms and their applications. The appropriate classification algorithm should be chosen according to different research purposes. Rough sets and cluster analysis are suitable for exploratory research without requiring a prior knowledge. Fuzzy sets theory, neural networks and decision tree are suitable for syndrome diagnostic criteria research when the classification goal is clear, because they require a prior knowledge. Among them, fuzzy sets theory could be used in combination with other classification algorithms. Thus, some new methods such as fuzzy clustering, fuzzy rough sets or fuzzy decision tree might be more suitable for TCM algorithm classification research. It is suggested that some novel classification algorithms need to be developed to fit the condition of TCM syndrome, based on the interdisciplinary theories and technologies.

In recent years,with the development of neuroimaging and the progress in related clinical studies,people have had a better understanding of posterior circulation ischemia.This article reviews the progress in the causes,mechanisms,clinical manifestations,diagnosis, treatment and prognosis of posterior circulation ischemia.

Corneal endothelial cell(CEC)is the most critical part for the cornea, of which activity can influence the postoperative vision.It is very important for the clinical cornea preservation considering the function and its self-purification of donor cornea.There are a variety of classical methods, which can significantly prolong the saving time of donor cornea with its good quality of CEC.We reviewed the published papers about present preservation methods of cornea, which can give us many suggestions for the clinical cornea preservation.

Objective To investigate the effect of Kangaroo care(KC)on diminishing neonatal pain during heel lancing. Methods Sixty neonates were divided randomly into two equal groups:intervention group and control group,which 30 cases in each group.In the intervention group,neonates were treated with KC(neonates were put in direct and continuous skin-to-skin contact with his/her mom)from 20 mins before heel lancing to 1 mins after the end of the lancing.In the control group,neonates were treated with regular care.The differences in heart rate,blood oxygen saturation,facial pain expression,crying duration and DAN(Douleur Aigue Nouveaune)scores between two groups were compared at 7 time points from 10s before the heel lancing to 10s after the lancing. Results The results of repeated measu te at 7 time points after heel lancing.There were significant interaction effect between time and main effect(P<0.01),which showed that the heart rate of two groups were not equal at different time points.Compared with the time effects(P<0.01)which showed that two groups of neonatal heart rate of the two groups would accelerate with time extension. There were significant differences(all P<0.05)in heart rate at 7 time points between two groups and smaller heart rate varied range in intervention group.The results of repeated measures analysis for neonatal blood oxygen saturation at 7 time points after heel lancing. There were significant interaction effect between time and main effects(P<0.01),which showed that the blood oxygen saturation of two groups were not equal at different time points.Compared with the time effect(P<0.01),which showed that two groups of neonatal blood oxygen saturation would reduce with time extension.There were significant differences(all P<0.05)in blood oxygen saturation of two groups at 30s,40s and 50s after heel lancing and smaller blood oxygen saturation varied range in intervention group.The crying duration and DAN scores in intervention group were significantly reduced compared with the controls(all P<0.05).Conclusions
Heel lancing could lead to moderate to severe pain of neonates.KC could effectively diminish the neonatal pain degree caused by heel lancing.

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Background:Damage of intestinal mucosal barrier is a key factor in the development and progress of acute pancreatitis(AP),and is closely related with the prognosis of the disease. Aims:To investigate the protective effect and possible mechanism of mucoprotective agent teprenone on intestinal mucosal barrier in rats with experimental AP. Methods:Forty-five adult male Sprague-Dawley rats were randomly divided into normal control group(n = 5),AP model group(n = 20)and teprenone treated group(n = 20). AP model was established by subcutaneous injection of cerulein at abdominal wall. Rats in treated group were intervened with teprenone intragastrically before and after model establishment. ELISA was used for measurement of serum interleukin-1(IL-1),IL-6,tumor necrosis factor-α(TNF-α)and amylase;histopathological and ultrastructural changes of small intestinal mucosa were observed by light microscope and transmission electron microscope;Western blotting was used to detect the expressions of tight junction protein occludin and ZO-1. Results:Serum levels of IL-1,IL-6,TNF-α and amylase in AP model group were significantly higher than those in normal control group(P < 0. 05),accompanied by necrosis and exfoliation of small intestinal villus,widening of intercellular tight junctions and downregulation of occludin and ZO-1 expression. While in teprenone treated group,serum levels of proinflammatory cytokines and amylase were significantly decreased as compared with AP model group(P < 0. 05),the villus of small intestine remained intact,and dense tight junctions were observed. Expressions of occludin and ZO-1 in teprenone treated group were upregulated. Conclusions:Teprenone may protect against intestinal mucosal barrier injury in AP model rats by upregulating tight junction protein expression.

Objective:To study the significance of CD55,CD59 and CD34 in the early diagnosis of aplastic anemia-paroxysmal nocturnal hemoglobi nuria(AA-PNH),and to search for a sensitive marker of the early diagnosis,so as to achieve the objective of early finding,early diagnosis and early treatment.Methods:CD55,CD59 and CD34 in AA-PNH patients were detected by Flow cytometry,the relationship among CD55,CD59 and CD34 in AA-PNH patients,PNH,AA patients and control group were studied to find out their statistic relevance.Results:The levels of CD55,CD59 and CD34 of PNH and AA-PNH were significantly lower than AA group and any other group and control group,the markers were positively correlated with hemolysis degree and were earlier than other markers of hemolysis.Conclusion:CD55,CD59 and CD34 antigens may act as the most sensitive marker of early diagnosis in PNH and AA-PNH syndrome,and have a close correlation with prognosis.