To improve the quality standard of Angelica sinensis, solve the problem of lacking relevant reference substance, a new method-based on the standard reference extract (SRE) was applied to achieve the quality control of Angelica sinensis. SRE of Angelica sinensis was obtained by chromatographic separation technology. After calibration of three makers of the SRE, an UPLC analytical method was developed to determinate the contents of the makers. T-test was used for comparison of the determination results of two methods (reference substances and SRE as reference, respectively), and the results demonstrated that there is no significant difference between the two methods. The presented method is very convenient and practical, which can be used for the quality control of Angelica sinensis.
Angelica sinensis ; chemistry ; Calibration ; Chromatography, High Pressure Liquid ; standards ; Drugs, Chinese Herbal ; chemistry ; standards ; Quality Control ; Reference Standards

Objective To investigate the phenomenon that SKOV3 was killed by the PBL mediated by BHL-Iin vitro and tlle possible related mechanisms.Methods MTr colorimetric assay was used to analyze in vitro if PBL is cytotoxicitic to tlle target cells at different ratios of activated PBL and target cells (25:1,12.5:1,6.25:1,3.2:1),BHL-I mediating time(24,36,48 h),targets(SKOV3,MCF-7)and concentration of BHL-I(100,50,25,12.5,6.25,3.2,1.6,Oμg/ml).The levels of perforin,GrB mRNA and the concentrations of hIFN-γand hTNF-α,which expressed and secreted by the effector(PBL)in the process of target cell being killed,were evaluated by RT-PCR or ELISA.respectively.Results The cytotoxicity of PBL to SKOV3 cells was more efficient than to MCF-7 cells(P<0.01),and tlle PBL exhibited most effective cytotoxicity to SKOV3 cells when the concent ration ratio of PBL to SKOV3 was 12.5:1.and the mediated time of 25 μg/ml BHL-1 was 36 h.Furthermore,The PBL expressed a hisher level of perforin,GrB mRNA and hTNFF-α,hIFN-γ when the BHL-I was present,and the IL-2 increased the level of these cytokines expressed by PBL.Condusion The anti-human ovarian carcinoma×anti-human CD3 bispecific single-chain antibody BHL-I can induce PBL efficiently to kill the SKOV3 cells containing related antigens.The cytotoxic activity may be associated with the eytokines(pefforin,GrB,hTNF-α,hIFN-γ)which expressed or secreted by activated PBL,while the IL-2 Call enhance the cytotoxicity of PBL mediated by BHL-I.

Objective To investigate the phenomenon that SKOV3 was killed by the PBL mediated by BHL-Iin vitro and tlle possible related mechanisms.Methods MTr colorimetric assay was used to analyze in vitro if PBL is cytotoxicitic to tlle target cells at different ratios of activated PBL and target cells (25:1,12.5:1,6.25:1,3.2:1),BHL-I mediating time(24,36,48 h),targets(SKOV3,MCF-7)and concentration of BHL-I(100,50,25,12.5,6.25,3.2,1.6,Oμg/ml).The levels of perforin,GrB mRNA and the concentrations of hIFN-γand hTNF-α,which expressed and secreted by the effector(PBL)in the process of target cell being killed,were evaluated by RT-PCR or ELISA.respectively.Results The cytotoxicity of PBL to SKOV3 cells was more efficient than to MCF-7 cells(P<0.01),and tlle PBL exhibited most effective cytotoxicity to SKOV3 cells when the concent ration ratio of PBL to SKOV3 was 12.5:1.and the mediated time of 25 μg/ml BHL-1 was 36 h.Furthermore,The PBL expressed a hisher level of perforin,GrB mRNA and hTNFF-α,hIFN-γ when the BHL-I was present,and the IL-2 increased the level of these cytokines expressed by PBL.Condusion The anti-human ovarian carcinoma×anti-human CD3 bispecific single-chain antibody BHL-I can induce PBL efficiently to kill the SKOV3 cells containing related antigens.The cytotoxic activity may be associated with the eytokines(pefforin,GrB,hTNF-α,hIFN-γ)which expressed or secreted by activated PBL,while the IL-2 Call enhance the cytotoxicity of PBL mediated by BHL-I.

OBJECTIVETo investigate the effects of Paecilomyces cicadae polysaccharide (PCPS) on the immunological function of aged rats in vivo.

METHODThe young and old rats were administered with normal saline as control groups, and the rats from test group were sc given 50, 100, 200 mg x kg(-1) x d(-1) dosage of PCPS for 3 weeks. The phagocytizing rate and index of PMphi, AMphi to S. aureas were observed, and the colorimetric MTI was used to analyze the proliferative activity of spleenocytes which had been stimulated with ConA or LPS. We also inspected the ability varing of ACP, LDH, ARG of spleen, and observed the ultramicro structure of spleen under the SEM.

RESULTThe phagocytosis of Mphi was lower in aged group than that in young' s group, and the proliferative activity of spleenocytes was lower too. The activities of ACP, LDH, ARG of spleen were extremely decreased (P < 0.01) in aged rats as well. The proliferative activity and phagocytotic rate were both extremely increased in PCPS groups (P < 0.01), and the mitochondrion and endoplasmic reticulum of spleen were accrementition as well (P < 0.01).

CONCLUSIONPCPS could enhance the phagocytizing function of PMphi, AMphi of aged rats in vivo, and strengthen the immune function of spleen and its proliferative activity as well. Then the immunity of aged rats could be improved. The PCPS may be an anti-aging agent.

Animals ; Hypocreales ; chemistry ; isolation & purification ; Immunity ; drug effects ; Male ; Microscopy, Electron, Transmission ; Polysaccharides ; pharmacology ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Spleen ; drug effects ; ultrastructure