Objective:To probe into clinical therapeutic effects of separated TCM compound recipe Xuefu Zhuyu Decoction for combination of disease and syndrome,so as to further expound the law of TCM recipe formulation.Methods:By using randomized double blind placebo control method,123 cases were randomly divided into Xuefu Zhuyu group,refine Xuefu Zhuyu group,drug-pair group and placebo group.Each ease was administered 10ml each time,thrice daily,4 weeks as one course.Results:The therapeutic effect for angina pectoris of coronary heart disease was in order of Xuefu Zhuyu group,refine Xuefu Zhuyu group,drug-pair group and placebo group(P

Objective: To study expression of CD44 S and CD44 V6 in ameloblastoma (AB) and odontogenic keratocyst (OKC) . Methods: S-P method was used to detect CD44 S protein in 77 cases of AB (32 of primary, 39 of recurrent,6 of malignant), 19 cases of OKC and 12 cases of oral normal mucosa;and CD44 V6 protein in 61 cases of AB (24 of primary , 31 of recurrent and 6 of malignant), 19 cases of OKC and 12 cases of oral normal mucosa. Results: Expression of CD44 S and CD44 V6 were detected in cell membrane of stratum spinosum and stratum basale in normal oral mucosa. In 19 cases of OKC, loss of expression of CD44 S was detected in 10 cases, and loss of integrity of CD44 V6 expression was detected in 15 cases. Loss ration of expression and abnormal expression ratio of CD44 S in AB were 27.3% and 63.6%, respectively. That of CD44 V6 in AB were 11.5% and 50.8% respectively. There was a signifcant difference between loss of expression or abnormal expression of CD44 S and CD44 V6 in AB, OKC and normal oral mucosa (P

Objective:To detect the expression of cyclin B 1 in human ameloblastoma(AB). Methods:Expression of cyclin B1 was detected in 73 cases of AB (32 primary, 33 recurrent and 8 malignant cases), 19 of odontogenic keratocyst (OKC) and 7 of normal oral mucosa by streptovidin-biotin method. Results:The ratio of positive nuclear expression of cyclin B 1 in normal oral mucosa, OKC and AB was 28.6%(2/7), 36.8%(7/19) and 63.0%(46/73) respectively (P

Objective:To investigate the expressions of epidermal growth factor receptor(EGFR) and C-erbB-2 in two salivary adenoid cystic carcinoma cell lines with different metastatic potential. Methods:Salivary adenoid cystic carcinoma SACC-83 cell line and its lung metastatic cell line SACC-LM were cultured in vitro, their cytoplasm and membrane samples were extracted, and the expressions of EGFR and C-erbB-2 were detected by Western blot, the results were quantitatively analyzed by FluorChem V2.0 software.Results:EGFR expression level in cell membrane was higher than that in cytoplasm(P0.05) in both cell lines.Conclusion:Higher EGFR expression in cytoplasm and higher C-erbB-2 expression may be related to the metastatic potential of SACC-LM cells.

Objective:To revise the McLean Screening Instrument for Borderline Personality Disorder(MSI-BPD)in China and explore the four-factor solution in Chinese non-clinical sample.Methods:A total of 1206 college students(749 males and 457 females) from Peking university at the mean age of 20.02?1.77 completed the measures successfully.Results:The MSI-BPD showed good internal consistency and concurrent validity.Confirmatory factor analysis confirmed the four-factor solution(emotional disregulation,impulsivity,cognitive disturbance,unstable interpersonal relationship) in our sample of Chinese college students.Conclusion:MSI-BPD is a valid measure in Chinese college students.Future research should be done for its use in Chinese clinical samples.

Objective To evaluate the clinical effect of ligation of the descending branch of uterine artery and compression sutures at the lower uterine segment in managing postpartum bleeding due to pernicious placenta previa. Methods Clinical data of 227 patients with pernicious placenta previa, admitted to Shenzhen Maternity and Child Healthcare Hospital between June 2010 and June 2013, were retrospectively analyzed. Eight-two women,who were admitted between June,2010 to December,2011, receiving B-Lynch sutures combined with uterine lower segment packing after delivering the baby and placenta during cesarean section were assigned as the control group. Another 145 cases, who were admitted between January 2012 to June 2013, receiving ligation of the descending branch of uterine artery and compression sutures at the lower uterine segment, were defined as the observational group. Several indicators during and after the operation were compared between the two groups. T-test or Chi-square test were applied for statistics. Results The duration of operation in the observational group was shorter than in the control [(92±26) vs (106±32) min, t=3.579, P<0.01]. The estimated blood loss during the operation, the total blood loss during the first 24 h after delivery and the blood loss during the operation for placenta percreta in the observational group were all lower than in the control [(1 230±481) vs (1 858±632) ml, (1 475±236) vs (2 482±364) ml, and (2 131±515) vs (2 587±498) ml, t=8.413, 25.295 and 6.484, all P<0.01]. The adjusted postoperative hemoglobin values of the observational group were significantly higher than that of control [(82±21) vs (69±19) g/L, t= - 4.634, P<0.01]. Besides, significant differences were found between the observational and control group on the rates of hysterectomy, blood transfusion, and uterine artery embolization [1%(2/145) vs 7%(6/82), 40%(58/145) vs 66%(54/82), 2%(3/145) vs 12%(10/82), χ2=5.408, 13.945 and 9.904, P < 0.05 or 0.01]. The dosage of Carboprost Tromethamine required was smaller in the observational group [(573±104) vs (729±128) μg, t=9.971, P<0.01]. Conclusions Ligation of descending branch of uterine artery and compression sutures in the lower uterine segment during cesarean section in women with pernicious placenta previa is an effective and simple method to control bleeding resulting reduction of blood loss and the hysterectomy rate.

To explore the way of quantified diagnosis of blood stasis syndrome (BSS) and the essence of BSS.

Objective To study the application value of breast gland needle aspiration pathology on the early diagnosis of breast cancer. Methods 1324 cases of breast swelling, tumor and spilling-over patients were diagnosed and differentiation diagnosed needle aspiration pathology in position using disposable plastic injector of 5-10 volume. And it was compared with clinical and pathology tissue, CT, super-sound , breast scanning, mini-nucleus ,tuberculosis antibody ,CA153 etc. Results Breast gland needle aspirate pathology has important application value not only on diagnosis breast diseases, but also in the early diagnosis and differentiation diagnosis of breast cancer. It has especial instructive significance in differentiating whether breast proliferation and glandular fibro adenoma transfer into cancer. Puncture success rate is high up to 100%,early diagnosis rate is 16.9%,the whole diagnosis correctness is 98.6%,which is highest historically. Conclusions Breast needle aspirate pathology has the merits of little injury, being simple and quick, safe and sound, economic and practical correct results. All items of technology index are evidently higher than traditional diagnosis methods. It can’t be replaced by any other methods by now and it has high spreading value.

Algorithms ; Noise, Occupational ; Numerical Analysis, Computer-Assisted

Objective　To conduct preliminary research on the prevalence and genotyping of urogenital Chlamydia trachomatis (CT) infection in sexually transmitted disease (STD) clinics in Hainan Province, to understand the epidemiological characteristics and genotype features of the population infected with urogenital CT, and to provide evidence for the formulation of scientific prevention strategies and measures. Methods　From 2018 to 2022, a total of 5 551 male urethral swabs and female cervical swabs were collected for detection of CT infection by real-time fluorescent quantitative polymerase chain reaction (RT-PCR). The OMPL gene was amplified from the DNA of some CT positive individuals by nested PCR, and the positive results were sequenced. Sequencing results were uploaded to BLAST website to find sequence similarity and construct a phylogenetic tree to determine the genotype. Results　Out of the 5 551 tested patients, 846 were positive for Chlamydia trachomatis infection, with a positivity rate of 15.2%, the positive detection rate of CT-DNA was 18.6% in male and 13.4% in female, the positive detection rate of male was higher than that of female. There were statistically significant differences in the CT-DNA positivite detection rate among different age groups (P<0.05), and the highest positivite rate CT-DNA was 58.0% in 20-<30 years old, while it was the lowest, at 1.0%, in those over 50; there were also significant differences in CT-DNA positivity detection rate between seasons (P<0.05), with the highest being 36.4% in the summer and the lowest at 9.6% in the winter. Genotyping of the CT-OMPL gene VS1-VS2 nucleotide sequence in some samples from the Hainan region identified six genotypes, including types D, E, G, F, J, and K, with type F being the main prevalent dominant type. Conclusions　CT infection in Hainan is associated with gender, age, and season, and the genotypes are diversified. It is necessary to further strengthen the screening of CT infection in the reproductive tract of men and women of childbearing age in the future STD prevention and treatment work to improve the fertility rate.