1.Application and progress of surgical nutrition
Chinese Journal of General Surgery 2001;0(08):-
Nutrition support is extensively used in many clinical fields. The rest energy requirement in Chinese is only 5021~5858kJ/d.In our country 40% to 50% of hospitalized patients need nutrition support.In nutrition support,attention should be paied to :(1)The energy giving to the patient should be appropriate;(2)hormone resistance;(3)immunonut rition.
2.Establishment of an orthotopic autologous liver transplantation model with bile ducts ischemia-reperfusion injury in rats
Academic Journal of Second Military Medical University 1981;0(04):-
Objective:To establish an orthotopic autologous liver transplantation model with bile duct ischemia-reperfusion injury in rats,so as to make it easy for the operation.Methods: Eighty SD rats were used to establish the models.The method used for this model was the same as the one for orthotopic liver transplantation,except for the blood vessel anastomosis and the constant pressure perfusion of portal vein and abdominal aorta.The portal vein and the hepatic artery reperfusion were controlled by artery clamp.Results: The successful rate of the operation for establishing the model was 95%(76/80) and the anhepatic phase was(16?2) min.The periods of hot and cold ischemia and reperfusion could be accurately controlled and the effect of double constant pressure perfusion was good.There was no erythrocyte in the capillary of bile ductal wall under the light microscope after the reperfusion.Conclusion: This technique,simulating the whole process of liver transplantation,is simple and has high successful rate.Besides,it rules out the influence of immune agent on bile ducts impairment,and better reflects the pathophysiological process of bile ducts ischemia and reperfusion injury,providing a novel approach for investigation of bile ducts injury during liver transplantation.
3. Determination of ropivacaine in rat plasma with liquid chromatography-tandem mass spectrometry
Journal of International Pharmaceutical Research 2016;43(4):736-739
Objective: To develop an LC-MS/MS method for the determination of ropivacaine in rat plasma. Methods: The chromatographic separation was achieved on an Agela MP C18(2.1 mm×50 mm,3 µm)analytical column. The multiple reaction monitoring(MRM) mode of the positive ion was adopted in the MS detection, and the precursors to the product ion transitions of m/z 275.2/126.1 and 326.0/291 were used to measure ropivacaine and internal standard (midazolam). Results: The method was linear over the ropivacaine concentration range of 0.5-250 ng/ml with the correlation coefficients r of 0.9946. The average inter-day precision values (RSD) were 1.32%-6.74%, the matrix effect values were 95.5%-102.6% and the average recovery values were 89.8%-104.7%. Conclusion: The analysis methods for ropivacaine are simple, accurate and sensitive, thus can be used for the pharmacokinetic research of ropivacaine incorporated cubic liquid crystal in rat.
4.Re-recognition and thinking of membrane anatomy.
Chinese Journal of Gastrointestinal Surgery 2021;24(12):1119-1121
5.Influence of relative warm ischemia on bile component in rats self-liver transplantation
Xuanlei REN ; Hongfeng ZHAO ; Jie ZHOU
Journal of Chinese Physician 2014;16(9):1204-1206
Objective To explore the effect of relative warm ischemia (RWI) on bile nature and component in rats self-liver transplantation.Methods Thirty two rats were randomly divided into group Ⅰ (control group),group Ⅱ (RWI 0 min),group Ⅲ (RWI 30 min),and group Ⅳ (RWI 60 min).The levels of bile phospholipids (PL),total bile acids (TBA),and PL/TBA ratio were detected early after self-liver transplantation.The concentration changes of hydrophilic and hydrophobic bile acids were examined.Results Only PL concentration of group Ⅱ seemed to be lower than that of group Ⅰ (P < 0.05),without any change for other indexes.Compared with group Ⅱ,only the PL and TBA concentrations of the group Ⅲ were decreased significantly (P < 0.05),and no significant difference was found between two groups on PL/TBA ratio and mole fractions of bile acids (P > 0.05).Compared to group Ⅱ,the concentrations of PL and TBA,PL/TBA ratio,and the mole fraction of taurocholic-β-mouse acid (T-β-MC) were much lower in group Ⅳ (P < 0.05),and the mole fraction of taurocholic acid (TC) were higher in group Ⅳ (P < 0.05).Conclusions RWI caused changes of PL/TBA ratio,hydrophilicity and hydrophobicity of bile early after liver transplantation,and the changes were directly proportional to the RWI time.The property of bile in early transplantation seemed not to be changed when the relative warm ischemia time was less than 30 min.When the RWI time was longer than 60 min,the bile component was changed obviously and the toxicity was increased.In liver transplantation,arterial anastomosis should be completed as soon as possible,and the RWI time should be less than 30 min.
6.Coal worker's pneumoconiosis with esophagitis of Candida albicans.
Yue-kun ZHAO ; Xuan ZHOU ; Jing ZHAO ; Feng-jie YU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(2):115-115
Aged
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Aged, 80 and over
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Candidiasis
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complications
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microbiology
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Coal Mining
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Esophagitis
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complications
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microbiology
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Humans
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Male
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Middle Aged
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Pneumoconiosis
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complications
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microbiology
7.Clinical effect of treatment for hand trauma with free flap of radial artery superficial palmar branch
Jie BAI ; Yuben XU ; Haizhen ZHOU ; Zhao TIAN ; Meili XING
Journal of Regional Anatomy and Operative Surgery 2017;26(6):435-437
Objective To investigate the clinical effect of the treatment for hand trauma with free flap of radial artery superficial palmar branch.Methods Selected 100 cases of patients with hand trauma who were treated in our hospital from January 2013 to December 2015.All the patients were treated with free flap of radial artery superficial palmar branch.The postoperative necrosis rate,survival rate,and infection rate of flap of all the patients were observed.All the patients were followed up for 6 months,and the healing of fracture were evaluated by X-ray examination so as to evaluate the hand function recovery.Results After the treatment,there were 9 cases of flap local necrosis and 10 cases of local infection,and the necrosis rate and infection rate were 9% and 10% respectively.The infection was controlled effectively after the symptomatic treatment and the flaps all survived.The swelling degree of skin flap was slight and the appearance was good.After 6 months of follow-up, the 100 cases all get epithelization completely.The X-ray examination showed that the fracture healing is good,with 7 cases of hook nail deformity and 5 cases of slow nail regeneration.The hand function recovery was excellent in 73 cases (73%),good in 16 cases (16%),and medium in 10 cases (10%).Conclusion It is an effective treatment for hand trauma with free flap of radial artery superficial palmar branch,as it can receive high flap survival rate and good recovery of hand function.
8.Efficacy and safety of amisulpride and clozapine in schizophrenia patients with predominantly negative symptoms
Jing LI ; Jie YUAN ; Xueli ZHOU ; Na ZHAO ; Yufei ZHANG
Drug Evaluation Research 2017;40(5):691-695
Objective To investigate the efficacy and safety of amisulpride and clozapine in schizophrenia patients with predominantly negative symptoms.Methods Totally 166 cases of schizophrenia patients with predominantly negative symptoms from May 2013 to May 2016 in The Sixth People's Hospital of Hebei were divided into observation group and control group,83 cases in each group.Patients in the observation group were treated with amisulpride,and control group were treated with clozapine.The clinical effect,SANS scores,and occurring rate of abnormal electrocardiogram were compared.Results The clinical effect,emotional insipid (blunting),and attention dysfunction scores from SANS of observation group were significantly better than those of control group on week 4,8,and 12,respectively (P < 0.05);The occurring rate of abnormal electrocardiogram in observation group was significantly lower than that of control group on week 12 (P < 0.05).Conclusion Compared with clozapine,amisulprid has better efficacy and safety in schizophrenia patients with predominantly negative symptoms,and can effectively improve of symptom of insipid (blunting) and attention dysfunction.
9.Relationship of lipid metabolism and homocysteine with hypertensive disorders in pregnancy
Hong LI ; Xin ZHAO ; Caimei HUANG ; Wenjuan ZHOU ; Jie ZHANG
Clinical Medicine of China 2012;28(8):824-827
Objective To explore the association of lipid metabolism and bomocysteine with hypertensive disorders in pregnancy and observe the inflammatory reaction and the pathological change of placenta.Methods We conducted a prospective randomized placebo-controlled study.Seventy-four cases with hypertensive disorders in pregnancy were recruited as the experimental group and 77 cases of normal pregnant women were recruited as matched control group from Aug.2008 to Mar.2010.The relationship between lipid metabolism and homocysteine was analyzed.Results There was significant difference between the two groups in body-mass index before pregnancy(29.03±4.52 vs.23.99±5.90,t =5.88),cholesterol[(6.16±1.48)mmol/L vs.(5.01±1.05) mmmol/L,t =5.52],low density lipoprotein[(3.46±1.35) mmol/L vs.(2.26±0.86) mmol/L,t =6.54]and C-reactive protein[(29.04±14.90) mmol/L vs.(15.48±4.57) mmol/L,t =7.62](P < 0.05).No significant difference was found on triglycerides[(3.29±1.03) mmol/L vs.(3.49±1.37) mmol/L],high density lipoprotein[(1.84±0.40) mmol/L vs.(1.88±0.35) mmol/L],homocysteine [(8.77±2.65) mmol/L vs.(8.40±2.03) mmol/L]and neonatal weight[(3547±519)g vs.(3431±461)g](P > 0.05).Significant difference exists in placenta pathology of infarction(54.55% vs.31.04%),villi dysplasia,fit nodules form and hypoxia(65.46% vs.39.66%)(P <0.05).Conclusion We should detect lipid,homocysteine and C-reactive protein by dynamic combinational ways,pay more attention to women with high BMI before pregnancy,and value placenta pathology research on hypertensive disorders in pregnant women.This has high clinical significance in revealing the etiology of hypertensive disorders in pregnant women and improving pregnant outcomes.
10.Exploration on standardized training program for residents in stomatology department
Jie ZHOU ; Liang ZHU ; Yunfu ZHAO ; Weilan SHAN
Chinese Journal of Medical Education Research 2012;11(8):852-854
Standardized training program for residents in Shanghai is one of the important measures in the health care reform.In accordance with Implementary methods of standardized training for residents in shanghai (for trial implementation),the department of stomatology in the affiliated Changzheng Hospital quickly initialized the standardized training program by defining the training objectives,establishing the training mechanisms,formulating the training contents and building the system of the assessment.Special attention was paid to improve the professional skill of the residents including ① clinical diagnostic ability; ② medical theoretical level and the medical thinking ability; ③ interpersonal communicational skill; ④ professionalism; ⑤ scientific research ability.Useful exploration was made to test the feasibility of the training program based on the practices and the effect was encouraging.