1.Application and progress of surgical nutrition
Chinese Journal of General Surgery 2001;0(08):-
Nutrition support is extensively used in many clinical fields. The rest energy requirement in Chinese is only 5021~5858kJ/d.In our country 40% to 50% of hospitalized patients need nutrition support.In nutrition support,attention should be paied to :(1)The energy giving to the patient should be appropriate;(2)hormone resistance;(3)immunonut rition.
2.Establishment of an orthotopic autologous liver transplantation model with bile ducts ischemia-reperfusion injury in rats
Academic Journal of Second Military Medical University 1981;0(04):-
Objective:To establish an orthotopic autologous liver transplantation model with bile duct ischemia-reperfusion injury in rats,so as to make it easy for the operation.Methods: Eighty SD rats were used to establish the models.The method used for this model was the same as the one for orthotopic liver transplantation,except for the blood vessel anastomosis and the constant pressure perfusion of portal vein and abdominal aorta.The portal vein and the hepatic artery reperfusion were controlled by artery clamp.Results: The successful rate of the operation for establishing the model was 95%(76/80) and the anhepatic phase was(16?2) min.The periods of hot and cold ischemia and reperfusion could be accurately controlled and the effect of double constant pressure perfusion was good.There was no erythrocyte in the capillary of bile ductal wall under the light microscope after the reperfusion.Conclusion: This technique,simulating the whole process of liver transplantation,is simple and has high successful rate.Besides,it rules out the influence of immune agent on bile ducts impairment,and better reflects the pathophysiological process of bile ducts ischemia and reperfusion injury,providing a novel approach for investigation of bile ducts injury during liver transplantation.
3.Influence of relative warm ischemia on bile component in rats self-liver transplantation
Xuanlei REN ; Hongfeng ZHAO ; Jie ZHOU
Journal of Chinese Physician 2014;16(9):1204-1206
Objective To explore the effect of relative warm ischemia (RWI) on bile nature and component in rats self-liver transplantation.Methods Thirty two rats were randomly divided into group Ⅰ (control group),group Ⅱ (RWI 0 min),group Ⅲ (RWI 30 min),and group Ⅳ (RWI 60 min).The levels of bile phospholipids (PL),total bile acids (TBA),and PL/TBA ratio were detected early after self-liver transplantation.The concentration changes of hydrophilic and hydrophobic bile acids were examined.Results Only PL concentration of group Ⅱ seemed to be lower than that of group Ⅰ (P < 0.05),without any change for other indexes.Compared with group Ⅱ,only the PL and TBA concentrations of the group Ⅲ were decreased significantly (P < 0.05),and no significant difference was found between two groups on PL/TBA ratio and mole fractions of bile acids (P > 0.05).Compared to group Ⅱ,the concentrations of PL and TBA,PL/TBA ratio,and the mole fraction of taurocholic-β-mouse acid (T-β-MC) were much lower in group Ⅳ (P < 0.05),and the mole fraction of taurocholic acid (TC) were higher in group Ⅳ (P < 0.05).Conclusions RWI caused changes of PL/TBA ratio,hydrophilicity and hydrophobicity of bile early after liver transplantation,and the changes were directly proportional to the RWI time.The property of bile in early transplantation seemed not to be changed when the relative warm ischemia time was less than 30 min.When the RWI time was longer than 60 min,the bile component was changed obviously and the toxicity was increased.In liver transplantation,arterial anastomosis should be completed as soon as possible,and the RWI time should be less than 30 min.
4. Determination of ropivacaine in rat plasma with liquid chromatography-tandem mass spectrometry
Journal of International Pharmaceutical Research 2016;43(4):736-739
Objective: To develop an LC-MS/MS method for the determination of ropivacaine in rat plasma. Methods: The chromatographic separation was achieved on an Agela MP C18(2.1 mm×50 mm,3 µm)analytical column. The multiple reaction monitoring(MRM) mode of the positive ion was adopted in the MS detection, and the precursors to the product ion transitions of m/z 275.2/126.1 and 326.0/291 were used to measure ropivacaine and internal standard (midazolam). Results: The method was linear over the ropivacaine concentration range of 0.5-250 ng/ml with the correlation coefficients r of 0.9946. The average inter-day precision values (RSD) were 1.32%-6.74%, the matrix effect values were 95.5%-102.6% and the average recovery values were 89.8%-104.7%. Conclusion: The analysis methods for ropivacaine are simple, accurate and sensitive, thus can be used for the pharmacokinetic research of ropivacaine incorporated cubic liquid crystal in rat.
5.Re-recognition and thinking of membrane anatomy.
Chinese Journal of Gastrointestinal Surgery 2021;24(12):1119-1121
6.Coal worker's pneumoconiosis with esophagitis of Candida albicans.
Yue-kun ZHAO ; Xuan ZHOU ; Jing ZHAO ; Feng-jie YU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2005;23(2):115-115
Aged
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Aged, 80 and over
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Candidiasis
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complications
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microbiology
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Coal Mining
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Esophagitis
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complications
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microbiology
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Humans
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Male
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Middle Aged
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Pneumoconiosis
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complications
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microbiology
7.Relationship of lipid metabolism and homocysteine with hypertensive disorders in pregnancy
Hong LI ; Xin ZHAO ; Caimei HUANG ; Wenjuan ZHOU ; Jie ZHANG
Clinical Medicine of China 2012;28(8):824-827
Objective To explore the association of lipid metabolism and bomocysteine with hypertensive disorders in pregnancy and observe the inflammatory reaction and the pathological change of placenta.Methods We conducted a prospective randomized placebo-controlled study.Seventy-four cases with hypertensive disorders in pregnancy were recruited as the experimental group and 77 cases of normal pregnant women were recruited as matched control group from Aug.2008 to Mar.2010.The relationship between lipid metabolism and homocysteine was analyzed.Results There was significant difference between the two groups in body-mass index before pregnancy(29.03±4.52 vs.23.99±5.90,t =5.88),cholesterol[(6.16±1.48)mmol/L vs.(5.01±1.05) mmmol/L,t =5.52],low density lipoprotein[(3.46±1.35) mmol/L vs.(2.26±0.86) mmol/L,t =6.54]and C-reactive protein[(29.04±14.90) mmol/L vs.(15.48±4.57) mmol/L,t =7.62](P < 0.05).No significant difference was found on triglycerides[(3.29±1.03) mmol/L vs.(3.49±1.37) mmol/L],high density lipoprotein[(1.84±0.40) mmol/L vs.(1.88±0.35) mmol/L],homocysteine [(8.77±2.65) mmol/L vs.(8.40±2.03) mmol/L]and neonatal weight[(3547±519)g vs.(3431±461)g](P > 0.05).Significant difference exists in placenta pathology of infarction(54.55% vs.31.04%),villi dysplasia,fit nodules form and hypoxia(65.46% vs.39.66%)(P <0.05).Conclusion We should detect lipid,homocysteine and C-reactive protein by dynamic combinational ways,pay more attention to women with high BMI before pregnancy,and value placenta pathology research on hypertensive disorders in pregnant women.This has high clinical significance in revealing the etiology of hypertensive disorders in pregnant women and improving pregnant outcomes.
8.Clinical application of respiratory navigator echo triggered black blood contrast cardiac MRI
Liuquan CHENG ; Qian ZHAO ; Na YANG ; Jie ZHOU ; Yi WANG
Chinese Journal of Radiology 2009;43(6):643-646
Objective To investigate the application of respiratory navigator echo triggered black blood contrast FSE in cardiac MRI. Methods The respiratory navigator echo trigger technique combining with black blood FSE (NAV-FSE) was tested on 11 volunteers and 5 patients in free breathing,using breath-hold FSE (BH-FSE) with the same imaging protocals as control. The imaging efficiency and the image sharpness were compared between NAV-FSE and BH-FSE and t-test was used for the statistics. Results All NAV-FSE acquisitions were completed in sixteen subjects while 4 BH-FSE acquisitions failed because of poor breath holding. The efficiencies of NAV-FSE were (42. 95±11.50)%, (56. 14±11.40)% and (55.25± 14. 70)% when echo train length (ETL) were 24, 16 and 8, respectively. When ETL were 16 and 24, the sharpness of NAV-FSE ( 0. 43±0. 02 vs 0. 36±0. 02 ) and BH-FSE ( 0. 36±0. 03 vs 0. 35±0. 02 ) were statistically different (t =4. 26, 5. 53 ,respectively; P <0. 05). NAV-FSE could have a shorter ETL setting without consideration of breath holding. Conclusion The navigator echo trigger technique could be compatible with black blood contrast FSE to image the heart without the restriction of breath holding and it allows to optimize the parameters to improve the image quality.
9.Comparative study on ischemia-reperfusion injuries caused by heterogeneity of different positions of biliary system in liver graft in rats
Hongfeng ZHAO ; Jie ZHOU ; Yu WANG ; Xuanlei REN
Chinese Journal of Hepatobiliary Surgery 2010;16(9):683-687
Objective To comparatively study the ischemia-reperfusion injuries caused by heterogeneity of different positions of the biliary system and different construction patterns of the peribiliary vascular plexus. Methods Thirty rats were randomly divided into 3 groups: Group Ⅰ , sham operated; Group Ⅱ , 1h ischemia in biliary tract followed by 1h reperfusion; Group Ⅲ, 1h ischemia in biliary tract followed by 2h reperfusion. TUNEL assay, pathomorphology score determination and ultrastructural quantitative analysis were performed on epithelium of the hilar bile duct, proximal common bile duct and interlobular bile duct. Results In groupⅡ , TUNEL assay and pathomorphology score showed no statistical difference between proximal common bile duct and interlobular bile duct (P>0.05) but showed significant differences in the hilar bile duct(P<0.05). Mean volume (V) of mitochondria and area density of microvilli were obviously serious in the hilar bile duct but obviously slight in the proximal common bile duct(P<0. 05). In group Ⅲ, the results of the above detections showed that the most severe was in hilar bile duct, followed by the interlobular bile duct and proximal common bile duct(P<0. 05). Conclusion Different injuries in various parts of the biliary system are caused by heterogeneity of biliary epithelial cells and construction patterns of the peribiliary vascular plexus. It also provides the experimental basis to explain the higher incidences of hilar bile duct stricture. It could be taken as the best position when the bile duct is anastomosed.
10.A new modified watershed algorithm for image segmentation in cervical cancer cell adhesion
Yinghong ZHAO ; Zhongshun ZHOU ; Cunjie SUN ; Jie PING
Military Medical Sciences 2014;(12):972-975
Objective To investigate an effective algorithm for image segmentation in cervical cancer cell adhesion , which enables accurate segmentation of the contour of adherent cells .Methods The images of target cells were extracted from the background area using level set methods , normalized with minimum values of transformation algorithms ,and multi-plied by the gradient image points in the region of interest ( ROI) to inhibit the undesired gradient information before the im-ages of adherent cells were segmented using labeled watershed algorithm .Results and Conclusion Compared to conven-tional watershed segmentation methods , this algorithm is not only effective in image segmentation of adherent cervical cancer cells with uneven staining and more accurate segmentation lines established around the contours of adherent cells , but of high clinical value .