Objective To evaluate the efficacy of staged nursing intervention on smoking behavior in young male adult patients with first attack of stroke.Methods 89 patients were randomly assigned to the intervention group (46 cases) and the control group (43 cases).In the intervention group,the patients were given staged nursing intervention based on their psychological stages in hospital and 11-week followup.The outcome of smoking cessation was evaluated three months after discharge.Results The rate of smoking cessation in the intervention group was higher than that of the control group.Smoking abstinence for the intervention group was more than the control group.FTND (Fagerstrom Test for Nicotine Dependence) scores were significantly different between two groups.Conclusions The staged nursing intervention is feasible for young male adults with stroke.We should increase secondary prevention consciousness and strengthen the implementation of relevant countermeasures.

Objective To explore the difference between before and afteintervertebral fusion in transforaminal lumbainter-body fusion (TLIF) by unilateral obilateral pedicle screw (PS) fixation .MethodThe L3 -5 Cdatbased on the normal people were used to rebuild the L3 -5 three-dimensional finite elemenmodel(INT) and TLIF operative model .On thibasi,the unilateral Pfixation (M1) and bilateral Pfixation (M2) modelbefore the intervertebral fusion and the unilateral Pfixation (M3) and bi-lateral Pfixation (M4) modelafteintervertebral fusion were buil.Aftesetting the boundary and loading the burden ,the ante-flexion ,retroversion ,lateral bending and rotation of lumbavertebrwere simulated and the L 4 -5 segmenanguladisplacemenand the stresdistribution of implanwere recorded .ResultThe unilateral and bilateral Pfixation model before fusion reduced the range of motion(ROM ) of segmencompared with the Inmodel ,the bilateral fixation wareduced greatethan the unilateral fixa-tion ,buthe difference aftefusion wasignificantly decreased .The peak Von Misestressebefore fusion in the unilateral Pfixa-tion wasignificantly highethan thain the bilateral fixation ,while theipeak Von Misestresseaftefusion trended to be con-sisten.Conclusion The unilateral Pfixation aftefusion can provide the consistensegmenstability same to the bilateral Pfixa-tion .The long-term effecof TLIF with unilateral Pfixation isimilato thawith the bilateral Pfixation .

Objective To explore the smoking behavior and its influencing factors of young male smokers with stroke,and set basis for further smoking cessation intervention.Methods 209 male smokers(≤45 years old) with stroke were randomly chosen,the information of demographic factors and smoking behaviors from medical files was got.Results Older people were more likely to be heavy smokers,the biggest percent (45.2％) of heavy smokers came from more than 40 years old people.Smoking consumption per day was affected by age,education and occupation,significant differences were found.Conclusions More than 40 years old patients in young male smokers with stroke should be focused in the intervention of smoking cessation.

Acute Disease ; Child ; Cytidine Deaminase ; genetics ; Humans ; Leukemia ; genetics ; Polymorphism, Single Nucleotide ; genetics

Child ; Child, Preschool ; Diagnostic Errors ; Female ; Humans ; Infant ; Infectious Mononucleosis ; diagnosis ; Male ; Suppuration ; diagnosis ; Tonsillitis ; diagnosis

Adolescent ; Adult ; Aged ; Burns ; etiology ; microbiology ; Child ; Child, Preschool ; Drug Resistance, Bacterial ; Explosions ; Female ; Humans ; Male ; Middle Aged ; Wound Infection ; microbiology ; Young Adult

Objective To investigate the efficacy and safety of immediate intravesical instillation combined with short-term maintained intravesical instillat.ion chemotherapy of pirarubicin (THP) after operation for preventing postoperative recurrence of superficial bladder tumor.Methods One hundred and seven patients with superficial bladder tumor were divided by random digits table method into two groups:group A (50 cases ) was treated with immediate intravesical instillation combined with short-term maintained intravesical instillation chemotherapy after operation,and group B (57 cases) was treated with conventional intravesical instillation chemotherapy.Patients in group A underwent intravesical instillation of THP within 24 hours,maintained intravesical instillation once a week for 8 weeks.Patients in group B underwent intravesical instillation of THP in one or two weeks after operation,once a week for 8 weeks.And then once a month for 10 months.All patients were followed up with regular cystoscopy examination.Results Patients were followed up for 13-84 months,the incidence of partial vesical toxic reaction in group A was 26.00 ％ (13/50),general symptom rate was 4.00％ ( 2/50 ),the rate of recurrency was 16.00％ ( 8/50 ).The incidence of partial vesical toxic reaction in group B was 22.81％( 13/57 ),general symptom rate was 1.75％( 1/57 ),the rate of recurrency was 19.30％ (11/57).There was no significant differences between two groups (P ＞ 0.05 ).Conclusions Immediate intravesical instillation of THP after operation is safe and effective,but it is not suitable for patients with obvious bladder perforation and large wound surface of bladder mucosa after transurethral rescection.Immediate intravesical instillation combined with short-term maintained intravesical instillation chemotherapy of THP after operation for preventing postoperative recurrence of superficial bladder tumor is effective and well tolerated.

Objective To investigate the renal function of the patients with acute myocardial infarction (AMI) and its effect on patients' outcome.Methods The renal function of 680 patients with AMI,received the conventional therapies, such as thrmbolysis, antiplatelet and antianginal therapies were investigated. According to it, 228 patients with renal dysfunction[GFR ＜90ml · min-1 · ( 1.73m2 ) -1]were divided into observation group,while the others[GFR≥90ml · min-1 · (1.73m2) -1]were divided into control group. To analyse their clinical characteristics、the inhospital mortality、one year mortality and the heart ocurrence rate in one year. Results Compared with the patients in the control group,the patients in the observation group were older、more likely to be women、and more likely to have hypertension、diabetes mellitus、coronary heart disease. The inhospital and one year mortality were higher ( 17. 8％ vs 9.74％ ,P ＜0. 01 ;25.00％ vs 14. 44％ ,P ＜0. 01 ) and the heart ocurrence rate was higher(57. 14％ vs38. 96％ ,P ＜ 0. 01 ) in one year in the observation group than that in the control group. ConclosionThe AMI patients with renal dysfunction were more likely to have concomitant diseases, worse ill condition and outcome. Renal dysfunction was an independent risk factor for the outcome in the patients with AMI.

Objective To analyse and monitor the genetic quality of closed colony NIH mice in Beijing district for the last 3 years.Methods We use biochemical genetic markers( including alkaline phosphatase -1 and the like 14 biochemical markers), selecting A and B colonies from different facilities for genetic monitoring in 2011 to study the polymorphism.And in 2014, 30 NIH mice just from B colony were monitored using the same testing and sampling methods.Results In 2011,NIH mice form both A and B facilities existed 6 polymorphic biochemical markers(Ce2,Car2, Gpi1,Es10,Gpd1,Pgm1); and NIH mice of B company also existed polymorphism in Es3 loucs.Between the 2 NIH mice colonies, there were significant difference in Es3、Gpd1、Pgm1 loci (P <0.01), and difference in Car2 locus(P <0.05).FST of the 2 colonies was 0.0406, the genetic identity was 0.9619, and the genetic distance was 0.0388.In B company, NIH mice of 2014 appeared 2 homozygous loci(Ce2 and Gpd1) when compared with NIH mice of 2011.Between the 2 NIH mice colonies, there were significant difference in Es10 and Gpd1 loci (P <0.01), and difference in Pgm1 locus(P <0.05).Fst of the 2 colonies was 0.1103, the genetic identity was 0.8847, and the Genetic distance was 0.1266.Conclusions Population isolation, breeding and selection, populationpopulation quantityquantity and generation significantly affected the genetic architecture of NIH mice.So when breeding and reserving seeds, we should strengthen the genetic monitoring of outbred NIH mice, in order to offer reliable genetic quality protection.

Objective To investigate the inhibitory effect of icariin(ICA) on the xenograft tumors growth of esophageal car‐cinoma and to preliminarily investigate its mechanism .Methods The MTT assay and Giemsa staining were applied to detect and observe the in vitro inhibitory effect of ICA on esophagus cancer cell lines Eca‐109 and TE‐13 .The xenograft tumor model of nude mouse esophagus cancer cell was constructed and divided into 3 groups ,6 cases in each group .Each mouse in the experimental group was intraperitoneally injected by ICA 50 mg/kg ;while the control group was injected by the same volume of normal saline and the positive control group was injected by cis‐platinum 2 mg/kg ,once every 2 days ,a total of 14 days .The tumor volume was measured once per 3 d .After experiment ,the tumor weight was measured;the TUNEL staining was used to observe the morphological chan‐ges and cell apoptosis of tumor tissue in each group .The changes of Fas and FasL protein expression in tumor tissues were analyzed by immunohistochemistry .The FasL and IFN‐γlevels in peripheral blood were tested by the ELISA assay .Results ICA exerted no obvious inhibitory effect on the proliferation of Eca‐109 and TE‐13 cell in vitro .The average volume and weight of xenografts tumor had statistical difference between the experimental group and the positive control group (P<0 .05) .The TUNEL staining results showed that the tumor tissues had obvious apoptosis ,the number of apoptosis cells was significantly increased compared with the control group(P<0 .05) .The immunohistochemistry experimental results showed that the expression of Fas and FasL was signifi‐cantly increased(P<0 .05) .The ELISA experimental results demonstrated that the FasL and IFN‐γlevels of peripheral blood in the experimental group were significantly increased(P<0 .05) .Conclusion ICA had no obvious inhibitory effect on esophageal cancer cell proliferation in vitro ,but could induce in vivo apoptosis through the Fas expression and secretion of FasL and IFN‐γ,thus plays the role of anti‐esophageal cancer .