1.Current status of surgical management of esophageal cancer in China and the future strategy.
You-Sheng MAO ; Jie HE ; Gui-Yu CHENG
Chinese Journal of Oncology 2010;32(6):401-404
China
;
Esophageal Neoplasms
;
pathology
;
surgery
;
Esophagectomy
;
methods
;
trends
;
Esophagoscopy
;
methods
;
Humans
;
Lymph Node Excision
;
methods
;
Survival Rate
4.Value of electronic colonoscopy in the diagnosis of childhood chronic diarrhea.
Jie-Yu YOU ; Chan-Bin CHEN ; Wen-Xian OU-YANG
Chinese Journal of Contemporary Pediatrics 2007;9(5):493-494
Adolescent
;
Child
;
Child, Preschool
;
Chronic Disease
;
Colonoscopy
;
methods
;
Diarrhea
;
diagnosis
;
pathology
;
Female
;
Humans
;
Infant
;
Infant, Newborn
;
Male
5.Anorectal motility in children with functional constipation.
Jie-Yu YOU ; Hong-Mei ZHAO ; Wen-Xian OU-YANG
Chinese Journal of Contemporary Pediatrics 2010;12(11):915-917
Adolescent
;
Anal Canal
;
physiopathology
;
Child
;
Constipation
;
physiopathology
;
Female
;
Gastrointestinal Motility
;
physiology
;
Humans
;
Male
;
Rectum
;
physiopathology
6.Coccidioidomycosis in an infant.
Wen-xian OUYANG ; Jie-yu YOU ; Yi-min ZHU
Chinese Journal of Pediatrics 2008;46(12):934-935
Coccidioides
;
Coccidioidomycosis
;
pathology
;
Humans
;
Infant
;
Male
7.HIGH LEVEL EXPRESSION AND ACTIVITY DETECTION OF SINGLE CHAIN IMMUNOTOXIN 183B_2ScFvPE38 AGAINST OVARIAN CARCINOMA
Fanglei YOU ; Jie FENG ; Yexia CHENG ; Tianyun FU ; Yu YAO ;
Acta Anatomica Sinica 1953;0(01):-
Objective To prepare the immunotoxin protein (183B 2ScFvPE38) which might be useful in immuno guided therapy for ovarian carcinoma and study the activity of the protein. Methods The methods of ELISA and cytotoxicity were used to study the immunotoxin after induced with IPTG and the activity of the immunotoxin. Results The expressed fusion proteins were detected mostly as inclusion bodies at high level, and soluble immunotoxins were also observed. The results showed liable activity of antibody part and toxic part. Conclusion The recombinant fusion protein 183B 2ScFvPE38 keeps the activity of both components and might be of great use in the future to deal with ovarian carcinoma. [
8.Effects of crocetin on VCAM-1 expression in human umbilical vein endothelial cells and monocyte-endothelial cell adhesion.
Shu-guo ZHENG ; Meng-qiu ZHAO ; You-nan REN ; Jie-ren YANG ; Zhi-yu QIAN
Acta Pharmaceutica Sinica 2015;50(1):34-38
Crocetin, a naturally occurring carotenoid, possesses antioxidant and antiatherosclerotic properties, of which the underlying mechanism remains unclear. In the present study, we examined the effects of crocetin (0.1, 1, 10 μmol·L(-1)) on angiotensin II (Ang II, 0.1 μmol·L(-1)) induced expression of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) and monocyte-endothelial cell adhesion. The effects of crocetin on the activation of nuclear factor kappa B (NF-κB) and intracellular reactive oxygen species (ROS) were also observed. The results demonstrated that crocetin notably suppressed Ang II induced NF-κB activation (P<0.01) and VCAM-1 expression (P<0.05, P<0.01) in HUVECs, accompanied by a markedly reduced monocyte-endothelial cell adhesion (P<0.05, P<0.01). In addition, preincubation with crocetin resulted in a significant enhancement of cellular antioxidant capacity (P<0.05, P<0.01), while Ang II induced intracellular ROS decreased markedly (P<0.05, P<0.01). These results indicated that crocetin was capable of suppressing Ang II induced VCAM-1 expression and monocyte-endothelial cell adhesion by suppression of NF-κB activation, which might be derived from the enhancement of antioxidant capacity and subsequent reduction of intracellular ROS.
Angiotensin II
;
metabolism
;
Antioxidants
;
pharmacology
;
Carotenoids
;
pharmacology
;
Cell Adhesion
;
drug effects
;
Human Umbilical Vein Endothelial Cells
;
cytology
;
drug effects
;
metabolism
;
Humans
;
Monocytes
;
cytology
;
NF-kappa B
;
metabolism
;
Reactive Oxygen Species
;
metabolism
;
Vascular Cell Adhesion Molecule-1
;
metabolism
10.Expression and Clinical Significance of Cyclin Kinase Inhibitor P21~(WAF1) and P27~(KIP1) in Children with Acute Leukemia
yong-chun, SU ; you-hua, XU ; jie, YU ; xiao-mei, LIU
Journal of Applied Clinical Pediatrics 1993;0(03):-
Objective To investigate the expression of cyclin kinase inhibitor P21~(WAF1) and P27~(KIP1)in children with acute leukemia and its clinical significance.Methods A total of 32 hospitalized children with acute leukemia(AL) were included in this study.Their bone marrow samples were collected before chemotherapy and individual patient was detected after complete remission(CR).The method of immunocytochemistry was used to estimate the expression of P21~(WAF1) and P27~(KIP1).Both positive percentage and intensity of the cells were counted.Results Findings showed that the positive ratios of P21~(WAF1) and P27~(KIP1) in total samples,ALL samples and AML samples were lower than the control group(P