1.Discussion on Treatment of Fire and Heat Disease by LIU Wansu Based on ‘Depression of Yang Qi’ Theory
Ting ZHAO ; Jie BAO ; Yongsheng FAN
Journal of Zhejiang Chinese Medical University 2017;41(7):553-555,561
[Objective]To explore LIU Wansu's methods in treating fire and heat disease from depression of yang qi.[Methods]To analyse LIU Wansu's understanding of fire and heat disease and highlight its treatment from depression of yang qi through reading his works like Exploration to Pathogenesis in Plain Questions,Yellow Emperor's Plain Questions on Xuanminglun Formulae and so on.[Results]LIU Wansu had profound medical knowledge,rich clinical experience and initiative spirit.Under the guidance of nineteen items of pathogenesis from Huangdi's Canon of Medicine,he expanded fire and heat disease and put up six exogenous pathogenic factors can transfer to fire theory.To be specific,excessive wind,cold,dampness and dryness would block Xuan Fu and depress yang qi,causing fire and heat disease.He regardeddepression of yang qi as a key link in six exogenous pathogenic factors' transferring to fire and put up cooling combined with ventilating method to dissipate depressed yang qi,including relieving exterior syndrome with pungent-cool drugs,clearing depressed heat,relieving exterior and interior syndrome,eliminating dampness and relieving depression as well as combination of cold and warm drugs.[Conclusion]LIU Wansu treated fire and heat disease from depression of yang qi,providing new methods in treating fire and heat disease for later generations as well as guiding significance in current clinical work.
2.Protective effects of MicroRNA-214 on myocardial injury induced by myocardial ischemia and reperfusion and the regulation mechanism of its downstream protein kinase
Shan LIU ; Yanqiu SONG ; Fumei ZHAO ; Ting LIU ; Jie GENG
Chinese Journal of Geriatrics 2017;36(7):802-805
Objective To investigate the protective effects of MicroRNA-214 on myocardial injury induced by myocardial ischemia and reperfusion,as well as the regulation mechanism of PI3K and its downstream protein kinase B(AKT)and FoxO1(PI3K / AKT / FoxO1).Methods Wistar rats were randomly divided into 4 groups:sham operation group(Sham group),myocardial ischemia reperfusion injury(IRI)group(IRI group),microRNA-214+sham operation group(MS group),microRNA-214+IRI group(MI group),(n=10,each).The cardiac function was detected at 6 h after ischemia-reperfusion operation.And blood lactate dehydrogenase(LDH),creatine kinase(CK),creatine phosphate kinase isoforms MB(CK-MB),cardiac troponin T(cTnT),serum B natriuretic peptide(pro-BNP)in plasma were detected by enzyme-linked immunosorbent assay(ELISA).Interleukin 10(IL-10),Interleukin 6(IL-6)and tumor necrosis factor α(TNF-α)were assayed.Pathological changes of myocardial tissue were detected by HE and Masson.The expression of microRNA-214 was detected by RT-PCR.The expression of Bax,Caspase-3,BCl2,PI3K,Akt,FoxO1 was detected by Western Blot.Results Compared with Sham group,IRI group showed a significantly increases in myocardial injury parameters of LDH,CK,IL-6 and TNF concentration in plasma,and a significantly reduced concentration of IL-10(P<0.05).And compared with Sham group,MI group showed a significantly increased expression of microRNA-214(P<0.05)and showed a significantly increased myocardial parameters of Bax,Caspase-3,PI3K,Akt protein,and a decreased level of BCl2,FoxO1(P<0.05).Compared with IRI group,microRNA-21 group showed a reduced myocardial ischemia-reperfusion-induced myocardial injury in rats and a reduced plasma concentration of LDH,CK,IL-6 and TNF-alpha,a inhibited expression of caspase-3,Bax,myocardial PI3K and Akt,and a promoted expression of BCl2 and FoxO1 protein(P<0.05).Conclusions MicroRNA-214 reduces the myocardial injury induced by myocardial ischemia-reperfusion through PI3K/Akt signaling pathway.
3.Frequency of tibial plateau fracture combined with dislocation and imaging features of medial or posteromedial fragments
Jun WANG ; Chunpeng ZHAO ; Ting LI ; Jie WEI ; Manyi WANG
Chinese Journal of Trauma 2015;31(5):427-430
Objective To evaluate the incidence and imaging features of tibial plateau fracture combined with dislocation.Methods A total of 298 patients with intact imaging data treated for tibial plateau fracture from December 2012 to June 2014 were analyzed retrospcctively.Tilt angle of medial fragment fracture line,ratio of width of medial fragment to total plateau,surface area percentage,fragment height,and major displacement were measured on CT images using picture archiving and communication system (PACS).Results Eighteen patients (6.0%) had tibial plateau fractures combined with dislocation,with tilt angle of medial or posteromedial fragment fracture line of (62.1 ± 14.1)°.Eleven patients (61%) had split of the medial tibial plateau with surface area percentage of (46.3 ± 2.5) %,fragment height of (43.9 ± 6.2) mm,and major displacement of (5.3 ± 2.1) mm.Seven patients (39%) had posteromedial fragment fracture with surface area percentage of (25.1 ± 5.7) %,fragment height of (40.0 ± 10.1) mm,and major displacement of (4.8 ± 6.45) mm.Conclusions Fracture line of fracture-dislocation tibial plateau fracture is much often through the eminence or located at the lateral eminence with large medial or posteromedial fragments.Surgical approaches and directions of nailing should be considered properly according to the morphological characteristics.
4.Sequence structure and phylogenetic analysis of the chloroplast genomes of Alangium chinense (Lour.) Harms and its different subspecies
Xiao-ying YANG ; Chang LIU ; Xian-fa ZENG ; Xiong-wei LIU ; Jie-hong ZHAO ; Ting-ting FENG ; Ying ZHOU
Acta Pharmaceutica Sinica 2022;57(10):3229-3239
italic>Alangium chinense is a commonly used medicinal plant of Alangiaceae
5.Absolute bioavailability of ginkgolide compounds in rats.
Hai-hong SI ; Ting GENG ; Xiao-ping SUN ; Jie ZHAO ; Jia XUE
China Journal of Chinese Materia Medica 2015;40(14):2882-2886
To investigate the pharmacokinetic characteristics and absolute bioavailability of ginkgolide A (GA), ginkgolide B (GB) and bilobalide (BB) in rats. In this experiment, a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/ MS) method was established to determine the plasma concentrations of GA, GB and BB in rats after rats were administrated with the three drugs through ig and iv respectively. The main pharmacokinetic parameters and absolute bioavailability of three ginkgolide compounds were obtained by using pharmacokinetic software DAS 2. 0. After the inject of GA, GB and BB, the results showed Cmax at (513.9 ± 116.9), (701.3 ± 76.0), (5,255.6 ± 476.8) µg · L(-1) and AUC0.24h of (960.9 ± 268.5), (779.5 ± 140.6), (7,409.3 ± 1,181.1) µg · h · L(-1), respectively; after the oral administration, the results showed Cmax at (522.9 ± 39.9), (146.8 ± 31.6), (2,711.9 ± 588.9) µg · L(-1) and AUC0-24 h of (1,760.4 ± 300.7), (636.6 ± 180.3), (16,651.4 ± 1,306.5) µg · h · L(-1), respectively. The absolute bioavailability of GA, GB and BB in rats was (61.1 ± 10.4)%, (27.2 ± 7.7)%, (56.2 ± 4.4)%, respectively. The method established in this experiment has a good specificity and sensitivity and so can be used to study the pharmacokinetics and absolute bioavailability of GA, GB and BB in rats.
Animals
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Biological Availability
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Chromatography, High Pressure Liquid
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Cyclopentanes
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pharmacokinetics
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Furans
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pharmacokinetics
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Ginkgolides
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pharmacokinetics
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Lactones
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pharmacokinetics
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Male
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Rats
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Rats, Sprague-Dawley
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Tandem Mass Spectrometry
6.The study of major histocompatibility complex class Ⅰ related genes expression in colonic mucosa tissue and the correlation with ulcerative colitis
Liuqing GE ; Ting JIANG ; Jie ZHAO ; Zhitao CHEN ; Feng ZHOU ; Bing XIA
Chinese Journal of Digestion 2010;30(11):811-813
Objective To investigate the function of major histocompatibility complex (MHC)class Ⅰ-related gene (MIC) in ulcerative colitis (UC) pathogenesis. Methods The difference of MICA, M ICB and their ligand NKG2D genes expression in colonic mucosa tissue of 34 UC patients and 12 healthy people were determined by fluorescent real-time reverse-transcriptase polymerase chain reaction (RT-PCR) and the expression location of M ICA in colonic mucosa tissue was obtained by laser scanning confocal microscope. Results The mRNA level of MICA, MICB and NKG2D expression in UC groups (3. 5408±2. 6658, 8. 9879±3. 2893 and 2. 4395±0. 8147 accordingly) was significantly higher than that in the healthy controls ( 1. 0477 ± 0. 7201, 4. 6293 ± 1. 2616 and 1. 1624±0. 3954 accordingly) (P = 0.0053, 0.0039 and 0. 0078 accordingly). It suggested that MICA was expressed in colonic epithelia cell membranes by laser scanning confocal microscopy.Conclusion The mRNA level of MICA, M ICB, and their ligand, NKG2D expression were all up regulated in the colonic mucosa of UC patients, which indicated MIC gene might perform important local function in UC.
7.An investigation on ABO blood type distribution and gene frequency of Buyi,Shui ethnic populations in Libo county of Guizhou province
Xiaomei ZHAO ; Yan HE ; Ting ZHANG ; Jie DENG ; Changxue WU ; Wenfeng YU ; Zhizhong GUAN
Chongqing Medicine 2016;45(9):1156-1158
Objective To understand the ABO blood type distribution of Buyi and Shui ethnic populations in Libo county of Guizhou province .Methods Totally 726 Buyi and 163 Shui individuals who were unrelated within three generations were detected ABO blood stype with glass-clotted method .Results The ABO blood type distributions in the Buyi and Shui ethnic populations both were in line with the Hardy-Weinberg equilibrium .The Buyi ethnic population ABO phenotypic distribution and gene frequen-cies were O>B>A>AB and r>q>p (r=0 .676 9 ,q=0 .176 7 ,P=0 .146 4) and the national index was 0 .842;while the Shui eth-nic population ABO phenotypic distribution and gene frequencies were O>A>B>AB and r>p>q(r=0 .522 6 ,q=0 .104 0 ,P=0 .164 7) and the national index was 1 .529 .Conclusion The Buyi and Shui ethnic populations′ABO blood type distribution in Libo county of Guizhou province is basically consistent with that of the same ethnic populations in other regions of Guizhou Province of the same ethnic groups .The genetic distance analysis prompts that the regional and ethnic differences exist in ABO blood type dis-tribution .
8.Neurotoxic effects of different concentrations of tetracaine and ropivacaine on brachial plexus nerve in rats
Jing LIU ; Ting WENG ; Zurong HU ; Weilu ZHAO ; Foquan LUO ; Jie JAI ; Jia MIN
Chinese Journal of Anesthesiology 2012;32(7):828-832
Objective To investigate the neurotoxic effects of different concentrations of tetracaine and ropivacaine on the brachial plexus nerve in rats.Methods Forty-eight male Sprague-Dawley rats,weighing 410-430 g,were randomly divided into 8 groups (n =6 each):normal saline group (group NS),0.25%,0.50% and 1.00% tetracaine groups (groups T1-3 ),and 0.25%,0.50%,1.00% and 2.00% ropivacaine groups (groups R1-4 ).The rats received injection of normal saline 1.0 ml,0.25%,0.50% and 1.00% tetracaine 0.5 ml,0.25%,0.50%,and 1.00% ropivacaine 1.0 ml and 2.00% ropivacaine 0.5 ml in groups NS,T1-3 and R1-4 respectively through one side of the axillary sheath.The other side of the axillary sheath served as control side.Five days later,compound action potential and nerve conduction velocity (NCV) of the brachial plexus nerve were measured.Tne brachial plexus nerve was obtained as the specimen for microscopic examination with light and transmission electron microscope.Results Compared with the control side and group NS,the compound action potential and NCV of the brachial plexus nerve were significantly decreased in groups T2,3 and R3,4 ( P < 0.05 ).The compound action potential and NCV of the brachial plexus nerve were gradually decreased with the increasing concentrations of tetracaine in groups T1 3 ( P < 0.05 ).The compound action potential and NCV of the brachial plexus nerve were significantly decreased in group R4 as compared with groups R1-3 (P < 0.05).The microscopic examination showed that the pathologic changes were more severe in groups T2,3 and R3,4 than those on the control side and than in group NS.Conclusion 0.50% and 1.00% tetracaine,and 1.00% and 2.00% ropivacaine can result in pathologic damage to the brachial plexus nerve in rats and the degree of damage is related to the concentration.
9.Practice and thought for quality control of drug clinical trial institution for clinical trials.
Ting ZHAO ; Ping WU ; Yong LI ; Feng-mei LIAN ; Ping LIU ; Jie QIAO
China Journal of Chinese Materia Medica 2014;39(24):4880-4882
The quality control of new drug cilnical trial is the effective guaranty for the pharmaceutical safety and effective after available on market. Enhancing the inspection and quality control of new drug clinical trials provide the crucial importance to achieve a persistent profitable standard. This paper mainly discussed the problems of current clinical trials based on annual check of drug clinical trial institution.
Clinical Trials as Topic
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Drug Evaluation
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Health Facilities
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Humans
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Quality Assurance, Health Care
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Quality Control
10.Effect of H2O2,extracellular matrix and out segment of photoreceptor on expression of focal adhesion kinase in RPE cell
Jie, ZHU ; Yu-sheng, WANG ; Wei, ZHAO ; Xiu-mei, YANG ; Xia, LI ; Ting-shuai, JIANG
Chinese Journal of Experimental Ophthalmology 2011;29(6):544-548
Background The underlying mechanism of choroidal neovascularization(CNV) is multifactorial and complex.Focal adhesion kinase(FAK) plays a crucial role in controlling essential cellular processes and influencing distinct steps of the angiogenic response.But to our knowledge,seldom study on the effect of FAK on CNV formation has been reported previously.Objective In this study,the effect of several CNV risk factors on the expression of FAK in cultured retinal pigment epithelium(RPE) cells was investigated to illuminate effect of FAK on CNV.Methods Human RPE cells were isolated from donor eyes and exposed to H2O2,swallow of outer segment of photoreceptors(POS) and extracellular matrix(ECM) separately with the treating as follows:RPE cells were co-cultured with 10,20,50 and 100μmol/L H2O2 for 20 days;POS(1×106/ml) were co-cultivated with RPE cells for 20 days(setting control group,POS group,hypoxia group with 200μmol/L CoCl2,and POS+hyoxia group);RPE cells were cultured on the plates coated with 100mg/L fibronectin(FN),laminin(LN) or collagen typeⅠfor 30minutes or 1 hour.The expression of FAK and pFAK in RPE cells were examined by Western blot analysis.Results FAK was highly expressed in the 20μmol/L and 50μmol/L H2O2 groups compared with control group(P<0.01);while he expression level of pFAK was reduced after treated with H2O2 in comparison with the control group(P<0.01).After cultured with POS for 20 days,the undigested lysosome could be observed in RPE cells.The expressions of FAK and pFAK in RPE cells were not significantly changed between control group and POS groups(P>0.05),but those in hypoxia group were significantly up-regulated in comparison with control group(P<0.01).Compared with the hypoxia group,the expression amount of pFAK was elevated in POS+hyoxia group(P<0.01).In comparison with control group,the increased pFAK expression was seen in FN,LN and collagen typeⅠtreating for 1-hour groups(P<0.05,P<0.01).Conclusion FAK pathway participates in several CNV-initiated signaling,such as H2O2,POS and ECM,in cultured RPE cells.It is reasonable to believe that FAK potentially plays an important role in CNV-dependent disorder.