1.The expression and distribution of protein translation initiation factors C2 gene in hepatocellular carcinoma and gastric cancer tissue
Qing ZHANG ; Jie LIU ; Li ZHANG
Chinese Journal of Digestion 1996;0(05):-
Objective To study the expression, distribution and significance of C2 gene in hepatocellular carcinoma (HCC) and gastric cancer tissue. Methods ABC immunohistochemical method was used to detect the expression of C2 protein in 60 samples of HCC tissue and 58 samples of gastric cancer tissue, and Western blot technique was used to detect the expression of C2 protein in 10 samples of HCC tissue and pericancerous tissue. Results 1. Expression of C2 protein in HCC tissue: in 60 samples of HCC and 42 samples of pericancerous tissue, the positivity rate of C2 protein was 27.3% and 83.3%, respectively. The positivity rate of C2 protein was significantly higher in pericancerous tissue than that in HCC tissue ( P
2.Clinical Study on Sanshen Granule Preparation Combined with MVP Regimen in Treating Middle-late Stage Non-Small Cell Lung Cancer
Wenshuo ZHAO ; Qing ZHANG ; Jie YU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(05):-
Objective To observe effect of Sanshen granule preparation combined with MVP regimen in treating middle-late stage non-small cell lung cancer patients. Methods 152 middle-late stage non-small cell lung cancer patients were randomly assigned to treatment group (79 patients) and control group (73 patients). Results Clinical beneficial rate (CR+PR+SD≥6 Months) were 82.3% and 64.4% respectively (P
3.Clinical Study on Advanced Colorectal Cancer Treated by Yiqi Huoxue TCM Combined with Chemotherapy
Qing ZHANG ; Wenshuo ZHAO ; Jie YU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(10):-
Objective To observe the effect of Yiqi Huoxue TCM combined with L-OHP+CF+5-FU regimen in treating advanced colorectal cancer patients. Methods 68 advanced colorectal cancer patients were randomly assigned to treatment group (38 patients, treated by Yiqi Huoxue TCM combined with L-OHP+CF+5-FU) and control group (30 patients, treated with L-OHP+CF+5-FU regimen). The therapeutic effects of two groups were observed. Result Clinical beneficial rate (CR+PR+SD) of treatment group and control group were 78.9% and 53.3% respectively (P
4.The relationship among the sense of coherence, empowerment and self-efficacy in peritoneal dialysis patients
Chunyan SUN ; Jie HAO ; Peng ZHANG ; Haiyan XU ; Qing ZHANG
Chinese Journal of Practical Nursing 2016;32(9):664-668
Objective To explore the relationship among the sense of coherence,empowerment and self-efficacy in peritoneal dialysis patients.Methods General information,the Sense of Coherence Scale,the Peritoneal Dialysis Empowerent Scale-Short Form and the Self-Efficacy for Managing Chronic Disease Scale were used to investigate a total of 160 peritoneal dialysis patients in a third-class hospital in Tianjin from March to June,2015.Results The mean score of the sense of coherence,empowerment,self-efficacy were (62.01±11.94) points,(4.09±0.68) points and (6.08±2.19) points.Both the sense of coherence and empowerment could positively influence the self-efficacy of peritoneal dialysis patients directly (r=0.429-0.571,P < 0.01),and the sense of coherence could also indirectly influence the self-efficacy through the mediation of empowerment (β decreased from 0.429 to 0.172,P < 0.01),the mediation effect accounted for 60.1% of the total effect.Conclusions Medical staff should take different actions to improve the sense of coherence and empowerment to improve the self-efficacy of peritoneal dialysis patients.
5.Low dose thalidomide combined with VAD regimen contained one-week dexamethasone on 42 cases of patients with multiple myeloma
Yanjun WANG ; Jie WU ; Qing DONG ; Ting WANG ; Xueyong ZHANG
Cancer Research and Clinic 2011;23(7):479-482
Objective To observe the efficacy and toxicity of low dose thalidomide combined with VAD regimen contained one-week dexamethasone in treatment on patients with multiple myeloma (MM). Methods 42 patients with MM were diagnosed in our hospital.The VAD regimen contained one-week dexamethasone was vincristine 0.4 mg/d, adramycin 10 mg/d and dexamethasone 40 mg/d for 4 days. At the same time, thalidomide is given 150-200 mg/d. Results The overall response rate (ORR) is 80.95%. Before and after treatment, all the detection indicators in MM patients were statistically different (P<0.001). There is small adverse reactions. Conclusion Low dose thalidomide combined with VAD regimen contained one-week dexamethasone in treatment of MM has more merits such as less side effects, good tolerance, convenient administration and significant curative effects, so it should be recommended in clinical works.
7.Effects of traditional Chinese herbal medicine on the neurobehavioral manifestations and the activity of dopamine D2 receptor in corpora striatum of rats with levodopa-induced dyskinesias
Canxing YUAN ; Qing YE ; Jie WANG ; Yan ZHANG ; Chonggang YUAN
Journal of Integrative Medicine 2008;6(10):1024-8
OBJECTIVE: To explore the effect of traditional Chinese herbal medicine (TCM) for nourishing liver and kidney, clearing meridians and removing toxic substances, on the neurobehavioral manifestations and the activity of the dopamine D2 receptor in rat with levodopa-induced dyskinesias (LID). METHODS: The rat model of Parkinson's disease (PD) was established by injecting 6-hydroxydopamine (6-OHDA) into right substantia nigra of brain, then, the model of LID in rat was produced by injecting levodopa (LD) and benserazide for 4 weeks. The rats were divided into normal control group, 4-week LD treated group, 4-week LD plus TCM treated group, 8-week LD treated group, and 8-week LD plus TCM treated group, and the effect of the TCM on neurobehavioral manifestations was observed. The radioligand binding assay (RLBA) and Scatchard drawing were used to measure the maximal binding capacity of receptor (Bmax) and equilibrium dissociation constant (KD) of the dopamine D2 receptor in corpora striatum. RESULTS: Compared with the 4-week LD treated group and 8-week LD treated group, TCM could decrease abnormal involuntary movement scores of the rats with LID; the RLBA revealed that the dopamine D2 receptor Bmax significantly increased (P<0.05, P<0.01) and the KD significantly decreased (P<0.05). CONCLUSION: TCM can improve the activity of the dopamine D2 receptor and relieve the symptoms of LID.
8.Effects of intelligent power-assisted functional electrical stimulation therapy on ankle joint function in post-stroke subjects
Guobao WANG ; Yong BAO ; Qing XIE ; Yi GAO ; Jie ZHANG
Chinese Journal of Physical Medicine and Rehabilitation 2014;36(7):529-531
Objective To observe the effect of power-assisted functional electrical stimulation (PAFES)therapy on ankle joint function recovery in stroke patients.Methods Ninety hemiplegic stroke patients were randomly and evenly divided into a control group,a PAFES group,and a neuromuscular electrical stimulation (NMES)group.All groups received conventional rehabilitation training.PAFES group adopted PAFES treatment on affected lower extremities and NMES group was given the NMES therapy on the tibialis anterior of the affected lower limbs,in addition to conventional rehabilitation training.The active range of motion (AROM) of ankle dorsiflexion,FuglMeyer motor assessment (FMA),Barthel index (BI) and Ankle flexion and extension movement (AFEM) in 10 seconds were evaluated before the trial and after 4 weeks treatment.Results After treatment,there were significant differences in the AROM of ankle dorsiflexion,FMA,BI and AFEM (P < 0.05) compared with before treatment within each group.The improvement of AROM of ankle dorsiflexion in PAFES group (8.19 ± 3.39) ° and the values in NMES group (8.96 ± 3.68) ° were to a significantly greater extend than control group (3.88 ± 4.10) ° (P <0.05) ; the improvement of FMA and BI in PAFES group was also superior to those in NMES group and control group (P < 0.05).Conclusion The intelligent PAFES therapy could help improve AROM of ankle dorsiflexion,the motor function of the affected lower extremity and the ability of the activities of daily living in stroke patients.
9.Related factors of liver cancer recurrence associated with hyperglycemia after radical resection of liver cancer
Qing CAI ; Shibo SUN ; Feng ZHONG ; Qifan ZHANG ; Jie ZHOU
Chinese Journal of Digestive Surgery 2016;15(1):47-52
Objective To investigate the related factors of early liver cancer recurrence associated with elevated fasting glucose levels after radical resection of liver cancer.Methods The retrospective cohort study was adopted.The clinical data of 145 patients with liver cancer who were admitted to the Nanfang Hospital of Southern Medical University from October 2009 to June 2013 werc collected.After radical resection of liver cancer, 111 patients with average level of fasting blood glucose (FBG) < 6.1 mmol/L and 34 patients with level of FBG ≥ 6.1 mmol/L were divided into the normal group and the hyperglycemic group, respectively.The blood glucose levels of patients during the period of hospital stay and follow-up were observed.The status of early recurrence and risk factors affecting recurrence in the 2 groups were analyzed.Follow-up of outpatient examination was applied to patients up to July 1, 2015 with the first recurrence as the end point.Patients had reexaminations once every month within postoperative month 6 and once every 3 months after 6 months.The general information [gender, age, FBG, underlying hepatopathy, preoperative AFP, alanine transaminase (ALT), aspartate aminotransferase (AST), Child-Pugh stage, Barcelona Clinic Liver Cancer (BCLC) stage, history of alcohol drinking], surgical data (surgical method, operation time, number of blood transfusion, volume of intraoperative blood loss), pathological data (histopathological differentiation, number of tumor nodules, diameter of maximal tumor, liver cirrhosis) and follow-up data (postoperative AFP, imaging findings, recurrence time, preventive chemotherapy) were collected.Measurement data with normal distribution were presented as-x ± s and analyzed by the t test.Measurement data with skewed distribution were presented as M (range) and analyzed by the non-parametric test.Count data were analyzed using the chi-square test.With the first recurrence as the end point, the tumor-free survival rate was drawn using the Kaplan-Meier method, and the comparison was analyzed by the logrank test.The risk factors affecting recurrence were analyzed using the Kaplan-Meier method for proportional hazards assumption test firstly, and then eligible factors were done using Log-rank test in the univariate analysis.The multivariate analysis was done using the COX model.Results The postoperative 1-, 2-year overall recurrence rates of liver cancer were 28.3% (41/145) and 45.5% (66/145).And 1-, 2-year recurrence rates of liver cancer were 21.6% (24/111) and 36.9% (41/111) in the normal group and 50.0% (17/34) and 73.5% (25/34) in the hyperglycemic group, respectively, showing significant differences between the 2 groups (x2=10.335, 14.053, P < 0.05).The univariate analysis showed that FBG, Child-Pugh stage, volume of intraoperative blood transfusion and postoperative AFP were risk factors affecting tumor-free survival rate after radical resection of liver cancer (x2 =17.591,6.492, 10.690,12.820, P < 0.05).The tumor-free survival rates at postoperative month 24 in the normal group and hyperglycemic group were 63.1% and 26.5% respectively, showing significant difference between the 2 groups (x2=17.591, P < 0.05).The results of multivariate analysis showed that level of FBG ≥6.1 mmol/L, volume of intraoperative blood transfusion > 200 mL and postoperative level of AFP > 8.1 pg/L were independent risk factors affecting tumor-free survival rate after radical resection of liver cancer (RR =2.542, 2.028, 2.724, 95 % condifence interval : 1.529-4.225,1.183-3.479,1.635-4.538, P < 0.05).Conclusions Elevated FBG level has a stimulative effect on early recurrence of tumor after radical resection of liver cancer.As a result, monitoring and controlling of blood glucose after operation is helpful in decreasing the early recurrence rate of patients with liver cancer.
10.The relationship between telomerase activity and the expression of proliferating cell nuclear antigen in oral squamous cell carcinoma
Guirong ZHANG ; Qing ZHOU ; Ming ZHONG ; Jie WANG ; Ruiwu LI
Journal of Practical Stomatology 2000;0(06):-
Objective:To study the relationship between telomerase activity and the expression of proliferating cell nuclear antigen(PCNA) in oral squamous cell carcinoma (OSCC). Methods:Telomerase activity and expression of PCNA in 68 cases of OSCC,34 cases of epithelium immediately adjacent to carcinomas and 12 cases of normal oral mucosa were detected by TRAP-PCR-ELISA and immunohistochemical SP method. Results:The positive rate of telomerase in OSCC, the epithelium immediately adjacent to carcinomas and the normal oral mucosa was 67.65%(46/68), 8.82%(3/34) and 0(0/12) respectively. Telomerase activity in OSCC was related to the grade of the clinicopathology(P