Objective To investigate the association of HLA DRB1 alleles with gastric carcinoma and H. pylori infection. Methods HLA DRB1 alleles polymorphism in 63 patients with gastric adenocarcinoma and 136 normal controls were tested with the polymerase chain reaction/sequence specific primer(PCR/SSP) technique. The status of H. pylori infection was determined by histology of gastric biopsy specimens and /or by ELISA. Results Both HLA DRB1 0901 and HLA DRB1 12 were positively associated, and HLA DRB1 15 was negatively associated with gastric adenocarcinoma, but no significant associations were found between patients with and without above HLA DRB1 alleles with regard to patients' age at presentation, sex ratio, tumor site (distal vs proximal), TNM staging, histological grading of tumor or status of H. pylori infection. Conclusions Our results suggest that HLA DRB1 0901, DRB1 12 and DRB1 15 are associated with gastric adenocarcinoma, but are not associated with the clinical features of gastric adenocarcinoma and the status of H. pylori infection.

BACKGROUND: High-altitude training before competition aims to obtain maximum hypoxia physiological adaptability of athletes through hypoxia environmental irritation at high altitude. Physiological adaptability to high al titude is the substantial foundation for function improvement of athletes,reflecting adjustment of body to environmental changes.OBJECTIVE: In views of self-regulation, practical training and compressed spectral array (CSA) electroencephalogram (EEG), and by observing adaptive change rule of brain before (before competition) and after high-altitude training of swimming athletes, the influence of high-altitude training was analyzed systematically on self-organizing state of athletes before competition so as to probe into the impacts of high-altitude training from brain level.DESIGN: Randomized investigation.SETTING: Guangdong Scientific Institute of Physical Exercise and Physical Exercise College of South China Normal University.PARTICIPANTS: Nine swimming athletes were selected from Guangdong Swimming Team from March to April 2001.METHODS: Time-order observation was applied in the experiment, in which, the results 1 week (3rd March) before high-altitude training, during (22nd March) and on the 5th day (5th April) after the training, the results in competition on the 13th day (12th April) after high-altitude training and corresponding blood lactic acid were tested and SCA EEG was done successively one day before high-altitude training (10th March), on the 1st day after the training (30th March) and on the 11th day after training, that was two days before National Competition (10th April) to observe self- adaptive regulation of brain to sports training load.MAIN OUTCOME MEASURES: ① Training index and lactic acid change before and during high-altitude training and before competition. ②Comparison of results before high-altitude training and in competition. ③Changes in indexes of cerebral evaluation before, during and after high-altitude training and before competition.RESULTS: ① Training results were changeable in high-altitude training.In national competition after high-altitude training, the scores of 6 cases of 10 person-times were reduced. Changes in blood lactic acid after high-altitude training were in conformity with training results. ② After high-altitude training, the average frequency of brain information distribution in athletes was shifted towards high frequency. Before the competition, negative entropy was lessened and information entropy was dispersed and the score in competition was declined.CONCLUSION: ① Cerebral order parameters of athletes constitute multicentralization, form the dispersion of technique control information and result in re-adjustment of brain function at matrix coordinating level, suggesting declining capacity to athletics. ② Effects of high-altitude training and maintaining of its functions are based on coincidence and continuity of arrangement of rise-fall training intensity of individual state and on the recovery and maintaining of the intensity at flatlands.

Objective To study the chemical constituents from mangrove Excoecaria agallocha.Methods The chemical constituents were separated and purified by chromatographic methods after solventextraction and were identified by spectroscopic analyses(EI-MS,1D NMR).Results Twelve compoundswere isolated from this plant and identified as:14-taraxeren-3-one(Ⅰ),dibutyl phthalate(Ⅱ),?-amyrin(Ⅲ),18-oleanen-3-ol(Ⅳ),18-oleanen-3-one(Ⅴ),phaeophytin A(Ⅵ),betulin(Ⅶ),?-rosasterol(Ⅷ),?-sitosterol(Ⅸ),betulinic aicd(Ⅹ),oleanolic acid(Ⅺ),ursolic acid(Ⅻ).Conclusion CompoundsⅦ,Ⅷ,Ⅹ,andⅫare isolated from this plant for the first time.

Objective To evaluate the condition of oxidative stress and immunosuppression in early stage of severe sepsis,and investigate the correlation between them. Methods A prospective random control study in-cluded patients group(n=51)and control group(n=31). The concentration of serum superoxide dismutase was measured by enzyme linked immunosorbent assay(ELISA),CD4+CD25+Treg% was measured by flow cytometry , respectively. The difference between two groups was compared and the correlation between parameters in patients group was evaluated. Results The concentration of serum SOD was lower than control group (P < 0.01). CD4+CD25+Treg% significantly high,compared to the control group(P < 0.01). There was no strong correlation be-tween parameters in patients group. Conclusion Oxidative stress and immunosuppression are exist in the early stage of severe sepsis.

Objective To study the effect of Alprostadil in the prevention of venous thrombosis among tumor patients after central venous catheter inserted through peripheral venous (PICC). Methods 137 patients with PICC were randomly divided into the control group and the experiment group. The control group received the routine nursing after indwelling catheter, while the experiment group received 10 ug of Alprostadi once a day for three days besides the routine nursing. Vascular ultrasonic examination was used to determine whether there was thrombosis among all of the patients after two months. The occurrence rates of thrombosis were compared between the two groups. Results The occurrence rate of thrombosis in the experiment group was lower than that in the control group (P<0.05). Conclusions Alprostadil can reduce the occurrence rate of venous thrombosis of tumor patients after PICC indwelling, so it can be an effective means for preventing thrombosis.

Objective: To investigate the protective effect of rhubarb (大黄) combined with scopolamine on gastric and gut mucosal barrier in patients with severe trauma. Methods: Forty patients with severe trauma, injury severity score (ISS)≥25, were randomly divided into two groups (each n=20), namely common therapy group (CM group) and rhubarb (infused into stomach) combined with scopolamine group (RS group). In RS group, rhubarb combined with scopolamine were given just after the first time measuring gastrointramucosal pH (pHi), the other treatment was the same as CM group.The mean arterial pressure (MAP) and heart rate (HR) were recorded, and pHi, lactic acid, interleukin-6 (IL-6) and tumor necrosis factor-? (TNF-?) were measured on admission and at 24, 48 and 72 hours after admission. Results: The MAP, HR and pHi were uptrend in both groups. There were significant increase in MAP and pHi of RS group at 72 hours compared with those of CM group (both P

AIM: To probe into the genetic susceptibility of HLA-DRB1 alleles to esophageal neoplasm in Hubei Han Chinese. METHODS: HLA-DRB1 gene polymorphism in 42 patients with esophageal neoplasm and 136 normal control subjects was studied by PCR and sequence. RESULTS: Allele frequency of HLA-DRB1 *0901 allele was significantly higher in esophageal cancer patients than those in normal controls (0 2500 vs 0 1397,P =0 028; the odds ratio 2 053; etiologic fraction 0 1282).There were no association between the rested HLA-DRB1 alleles with patients. CONCLUSION: Individuals carrying HLA-DRB1 *0901 may be susceptible to esophagealo carcinoma, its nucleotide sepuence approachs to the corresponded allele sequence(exon 2)published in GenBank.

Objectives To investigate the expression of matrix metalloproteinase-9 (MMP-9) and its tissue inhibitor (TIMP-1) in cutaneous squamous cell carcinomas (SCC) and the relationship between the expression and invasive growth and metastasis of SCC. Methods Immunohistochemical method of streptavidin-peroxidase (SP) was used to detect the expression of MMP-9 and TIMP-1 proteins on the paraffin embedded sections of 65 patients with cutaneous SCC and 5 cases of normal epidermis. The immunohistochemical results were analyzed quantitatively using an image analysis system. Results MMP-9 and TIMP-1 proteins were diffusely expressed on the tumor nests and the mesenchymal cells around the nests, while in normal epidermis MMP-9 and TIMP-1 were weakly positive. The expression level of MMP-9 protein and the ratio of MMP-9/TIMP-1 were higher in aggressive SCC group than those SCC in situ group (t = 2.33 and 2.36, respectively, P

Objective To observe the effect of pulsed electromagnetic fields(PEMFs)of different daily treatment durations on biomechanical properties of femur in ovariectomized rats,so as to find out the optimal daily treatment time.Methods Fifty female Sprague-Dawley rats were randomly divided into five groups:(1)SHAM control(no PEMFs treatment),(2)OVX control(no PEMFs treatment),(3)OVX I(PEMFs treatment at 8Hz fre- quency with 3.8 mT intensity,20 min daily for 30 days),(4)OVX 11(PEMFs treatment at 8 Hz frequency with 3.8 mT intensity,40 min daily for 30 days),and(5)OVXⅢ(PEMFs treatment at 8Hz frequency with 3.8 mT in- tensity,60 rain daily for 30 days).All the rats were subject to bilateral overiectomy except those in the SHAM control group.The biomechanical properties of the femur were assessed after 30 days of PEMFs treatment.Results The values of the parameters of the biomechanical properties obtained with the OVX control group were significantly lower than those of the other 4 groups(P0.05).Conclusion PEMFs of the three different daily treatment durations can maintain the biomechanical properties of the femur in ovariectomized rats.Under certain in- tensity(3.8 mT)and frequency(8 Hz),PEMFs of the three different treatment durations can significantly maintain biomechanical properties of femur in ovariectomized rats approximately to the nomal level,but among the three groups,the difference is not significant.It was shown that exposure to PEMFs for 20 to 60 minutes daily had similar effect of maintaining biomechanical properties of the femur in ovariectomized rats.

Humans ; Intestinal Obstruction/complications* ; Ileus/etiology* ; Abdominal Injuries/complications*