OBJECTIVETo study the over-expression of mutant p53 protein in non-mucinous adenocarcinoma in-situ (NMAIS) and invasive adenocarcinoma, NOS of lung.

METHODSImmunohistochemical study for p53 protein was performed on 17 cases of NMAIS and 70 cases of invasive adenocarcinoma, NOS of lung. The difference in p53 over-expression between the two tumor subtypes was analyzed.

RESULTSThe over-expression of mutant p53 protein was observed in 0 case (0%) of NMAIS and 37 cases (52.9%) of invasive adenocarcinoma, NOS of lung. The difference was of statistical significance (P = 0.000).

CONCLUSIONMutant p53 protein over-expression may play a role in the progression of NMAIS to invasive adenocarcinoma, NOS.

Adenocarcinoma ; metabolism ; Adenocarcinoma in Situ ; metabolism ; Humans ; Immunohistochemistry ; Mutant Proteins ; genetics ; metabolism ; Tumor Suppressor Protein p53 ; genetics ; metabolism

Objective： The current study was designed to investigate the expression of p73 gene in lung cancer tissue. Methods： The authors detected quantitatively p73 gene expression in 40 specimens of lung cancer and matched paracancer tissue by RT PCR and analyzed the alteration of p73 by PCR and SSCP. Results： ① Using RT PCR, 24 specimens of lung cancer were determined to be overexpression of p73, 9 of which were moderately differentiated, 15 of which were poor differentiated; 9 of which were Stage Ⅰ-Ⅱ , 15 of which were Stage Ⅲ-Ⅳ . No specimen of paracancer tissue was screened out to be overexpression of p73. There was significantly different expression rates between lung cancer and paracancer tissue (P< 0.01). ② Allele specific analysis showed that expression of G/C： A/T appeared in 10 of 12 heterozygous lung cancer specimens and expression of G/C in 27 specimens. ③ No mutation was detected by SSCP. Conclusion： The expression of p73 gene may be related to differentiation level of lung cancer but not related to clinical stage.

OBJECTIVETo inquire into the therapeutic effectiveness of free iliac crest grafts with periosteum on old acetabular defects.

METHODSFrom February 1996 to June 2005, 9 patients were treated with free iliac crest grafts with periosteum to reconstruct old acetabular defects. There were 7 males and 2 females and the average age was 41.3 years. The acetabular defects were caused by traffic accidents in 6 cases and fall injury in 3 cases. The time from injury to treatment was 4-13 months and averaged 8 months. Intraoperatively we firstly removed the acetabular fracture fragments of the posterior wall. The femoral head was then reduced. Bone graft was harvested from the iliac crest with periosteum, which was sculpted with a rongeur to conform to the defect. The concave (iliac fossa) side of the graft was placed toward the femoral head. The graft was securely fixed by two to three leg screws.

RESULTSPostoperative syndrome was not found in any of the cases. Harris' score system showed that the score raised from 32.3 points preoperatively to 81 points postoperatively. The hip function was evaluated as excellent in 3 cases, good in 4 cases and fair in 2 cases.

CONCLUSIONSAlthough this procedure could not exactly reproduce the anatomy of the hip joint, it enables to restore the posterior stability, provide bone-stock for the hip joints and prevent dislocation of the femoral head.

Acetabulum ; injuries ; surgery ; Adult ; Bone Regeneration ; Cartilage, Articular ; physiology ; Female ; Humans ; Ilium ; transplantation ; Male ; Middle Aged ; Periosteum ; transplantation

Objective To study the over-expression of mutant p53 protein in non-mucinous adenocarcinoma in-situ ( NMAIS ) and invasive adenocarcinoma, NOS of lung. Methods Immunohistochemical study for p53 protein was performed on 17 cases of NMAIS and 70 cases of invasive adenocarcinoma, NOS of lung.The difference in p53 over-expression between the two tumor subtypes was analyzed.Results The over-expression of mutant p53 protein was observed in 0 case (0%) of NMAIS and 37 cases (52.9%) of invasive adenocarcinoma, NOS of lung.The difference was of statistical significance (P =0.000).Conclusion Mutant p53 protein over-expression may play a role in the progression of NMAIS to invasive adenocarcinoma, NOS.

BACKGROUNDHyperglycemia has been shown to be a powerful predictor of poor outcome after ST-segment elevation myocardial infarction (STEMI). This study aimed to evaluate the effect of admission glucose on microvascular flow after successful primary percutaneous coronary intervention (PCI) in patients with STEMI.

METHODSSuccessful primary PCI was performed in 267 patients with STEMI. The maximum ST elevation of single electrocardiogram (ECG) lead before and 60 minutes after PCI was measured, and patients were then divided into 3 groups according to the degree of ST-segment resolution (STR): absent (<30%), partial (30% to 70%) or complete (> or =70%).

RESULTSOf the 267 patients, 48 (18.0%) had absent STR, 137 (51.3%) experienced partial STR, and 82 (30.7%) had complete STR. The degree of STR decreased with increasing admission glucose levels (P=0.032), and patients with hyperglycemia (serum glucose level > or =11 mmol/L) were more likely to have absent STR (P=0.001). Moreover,hyperglycemia was an independent predictor of incomplete STR (odds ratio, 1.870; 95% confidence interval, 1.038 to 3.371, P=0.037).

CONCLUSIONSHyperglycemia on admission is associated with abnormal coronary microvascular reperfusion in patients with STEMI after successful primary PCI, which may contribute, at least in part, to the poor outcomes in these patients.

Adult ; Aged ; Angioplasty, Balloon, Coronary ; methods ; Electrocardiography ; Female ; Glucose ; metabolism ; Humans ; Hyperglycemia ; blood ; pathology ; physiopathology ; Male ; Middle Aged ; Myocardial Infarction ; blood ; physiopathology ; therapy ; Odds Ratio

BACKGROUNDPrevious studies have suggested that hypothyroidism correlated with coronary heart diseases (CHD) mortality in long-term cohort, but whether the thyroid function status is associated with myocardial injury in acute ST-elevation myocardial infarction (STEMI) has not been investigated sufficiently.

METHODSFive hundred and eighty-two hospitalized patients from January 2010 to December 2011, with the diagnosis of STEMI, were enrolled in this study. All patients underwent testing for thyroid function status, cardiac troponin I (cTnI), cardiac enzymes, C-reactive protein (CRP). We investigated the association between thyroid hormone levels and cardiac markers (creatine kinase-MB and cTnI), and thus evaluated the potential role of thyroid function status in predicting the myocardial injury.

RESULTSThere were 76 patients (13.06%) who had hypothyroidism including low-T3-syndrome (34 patients, 5.84%), subclinical hypothyroidism (28 patients, 4.81%) and clinical hypothyroidism (14 patients, 2.41%). After adjusting for conventional risk factors (age, gender, smoking, diabetes mellitus, dyslipidemia, hypertension), free triiodothyronine (FT3) was significantly and negatively correlated with log-CKMB (r = -0.244, P < 0.001) and log-cTnI (r = -0.290, P < 0.001), indicating that the lower thyroid hormone level correlates with the severer cardiac injury in STEMI patients. FT3 also had a moderate negative correlation with CRP (r = -0.475, P < 0.001), which might indicate that hypothyroidism may activate the inflammation response. No significant correlation was found between other thyroid parameters (TSH, FT4) and cardiac markers.

CONCLUSIONSAs the lower FT3 level correlates with higher level of cardiac markers and lower left ventricular ejection fraction (LVEF), the hypothyroidism may be a predictor for myocardial injury in STEMI. And these results may warrant further study to investigate whether reversing the hypothyroidism could benefit the STEMI patients.

Aged ; C-Reactive Protein ; metabolism ; Echocardiography ; Female ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; blood ; Myocardium ; metabolism ; pathology ; Thyroid Gland ; metabolism ; Triiodothyronine ; blood ; Troponin I ; metabolism

AIMTo investigate the antitumor immunity by a dendritic cell (DC) vaccine encoding secondary lymphoid chemokine gene and tumor lysate on murine prostate cancer.

METHODSDC from bone marrow of C57BL/6 were transfected with a plasmid vector expressing secondary lymphoid chemokine (SLC) cDNA by Lipofectamine 2,000 liposome and tumor lysate. Total RNA extracted from SLC+lysate-DC was used to verify the expression of SLC by reverse transcriptase-polymerase chain reaction (RT-PCR). The immunotherapeutic effect of DC vaccine on murine prostate cancer was assessed.

RESULTSWe found that in the prostate tumor model of C57BL/6 mice, the administration of SLC+lysate-DC inhibited tumor growth most significantly when compared with SLC-DC, lysate-DC, DC or phosphate buffer solution (PBS) counterparts (P < 0.01). Immunohistochemical fluorescent staining analysis showed the infiltration of more CD4(+), CD8(+) T cell and CD11c(+) DC within established tumor treated by SLC+lysate-DC vaccine than other DC vaccines (P < 0.01).

CONCLUSIONDC vaccine encoding secondary lymphoid chemokine and tumor lysate can elicit significant antitumor immunity by infiltration of CD4(+), CD8(+) T cell and DC, which might provide a potential immunotherapy method for prostate cancer.

Animals ; Antibodies, Neoplasm ; biosynthesis ; Antigens, Neoplasm ; immunology ; CD11 Antigens ; immunology ; Cancer Vaccines ; immunology ; therapeutic use ; Cell Line ; Chemokines ; biosynthesis ; Dendritic Cells ; immunology ; metabolism ; Epitopes ; immunology ; Fluorescent Antibody Technique ; Killer Cells, Natural ; immunology ; Lymphocytes ; metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Neoplasm Transplantation ; Plasmids ; genetics ; Prostatic Neoplasms ; immunology ; pathology ; prevention & control ; Reverse Transcriptase Polymerase Chain Reaction ; T-Lymphocytes ; immunology

OBJECTIVETo investigate uptake and accumulation of gentamicin by cells in the guinea pig inner ear after intratympanic injection using a fluorescent probe--gentamicin-Texas-red conjunction (GTTR).

METHODSAdult guinea pigs (n = 80) were administered a single dose of GTrR to the middle ear cavity through the intact membrane and survived for 12 h, 24 h, 48 h, 3 d, 4 d, 7 d, 14 d and 28 d. The distribution of GTTR in the cochlear and vestibular cells was observed after staining with phalloidin-alexa-488. Texas Red and DMSO were injected into the tympanum as control.

RESULTSDiffuse staining of gentamicin in the labyrinth was observed initially after local drug administration. At later time point the outer hair cells and sensory cells of vestibular organ were staining more densely than the support cells in the inner ear. The peak level of fluorescent density was reached 3 days after local injection. The GTTR was observed in the infracuticular zone.

CONCLUSIONSGTTR was a potential fluorescent probe to investigate the pharmacokinetics and mechanisms of gentamicin accumulation in local application.

Animals ; Anti-Bacterial Agents ; administration & dosage ; pharmacokinetics ; toxicity ; Ear, Inner ; metabolism ; Fluorescent Dyes ; Gentamicins ; administration & dosage ; pharmacokinetics ; toxicity ; Guinea Pigs ; Hair Cells, Auditory ; metabolism

Objective To investigate the uptaking and accumulation of gentamicin by mouse hair cells in vitro.Methods Cochlear explants were prepared from the microdissected neonatM mouse cochlea. Cochlear explants were cultured with gentamicin-Texas-red conjunction (G1TrR) for difierent time.Laser confocal microscopy was used to observe the distribution of GTYR in the cochlear sensoty ceUs after labeling with phalloidin-alexa-488.ResulIs Soon after culture,there was diffuse red staining all tissue cells in the explants.At later time the hair cells were more staining tllan other cells in the explants.There was no obviously accumulation of GTTR in the supporting cells.The peak level of fluorescent density was reached at 24 hours culture.The GTTR was seen in the infracuticular zone of the hair cells.There was still accumulation of GTTR in the hair cells of the explants after 7 days culturing.Conclusions GTTR and Cochlea explants were useful methods to investigate the pharmacokinetics and mechanisms of gentamicin accumulation over time.

Objective To screen the MYBPC3 gene mutations in Han Chinese patients with hypertrophic cardiomyopathy ( HCM ). Methods Sixty-six patients with HCM were enrolled for the study. The exons in the functional regions of MYBPC3 were amplified with PCR and the products were sequenced. Results Four novel mutations and four common polymorphisms were identified in this patient cohort. A Lys301fs mutation in exon10 was evidenced in a H30, and when he was 47 years old, he had the chest tightness, shortness of breath with septal hypertrophy of 18. 7mm; a Asp463stop mutation in exonl7 was detected in a H48, he was 24 years old 24-year-old when a medical examination showed ventricular septal hypertrophy of 15.4 mm; both Gly523Arg mutation in exonl8 and Tyr847His mutation in exon26 were found in a H53 with onset age 36 years old, feeling chest tightness after excise and his ventricular septal hypertrophy was 27 mm that time. MYBPC3 mutatons occurred in 4. 5% patients in this cohort. These mutations were not found in 100 non-HCM control patients. Conclusion MYBPC3 mutation is presented in a small portion of Han Chinese patients with HCM.