There should be confusion about diagnosis and treatment for multiple segments cervical myelopathy in some respects. The author reviewed the literature and combined with clinical experience, proposed a new classification for cervical myelopathy according to responsibility segment areas, which dividing into single segment,double segments (continues or jumping type), multi-segment (≥ three segments). The responsible segments determination is the premise of diagnosis and also a key to determine surgical decompression segment. Decompression only according to imaging was not desirable, surgical segment should mainly relies on clinical, imaging, electrophysiological and comprehensive analysis to avoid surgery range expansion. Surgical approach and procedures are still the focus and hotspot of cervical myelopathy treatment, and no treatment standards and corresponding guidelines to consult. The author proposes that surgical approach should advocate the individual, and surgical procedure should follow simple instead of complicate, anterior and posterior combined decompression is not necessary in most cases, and anterior and posterior fixation are not need.
Cervical Vertebrae ; surgery ; Decompression, Surgical ; Humans ; Spondylosis ; diagnosis ; surgery ; Treatment Outcome

Objective To evaluate the effectiveness of TCM combined with western medicine for advanced gastric cancer.Methods We retrieved literatures of randomized controlled clinical trials related (from January 1991 to June 2012) to the use of TCM combined with western medicine treatment for advanced gastric cancer and made meta-analysis including:the effectiveness,Kamofsky scores and publication bias.Results 44 papers (including 3088 AGC patients) were included.Meta analysis suggested a difference between the treatment group and the control group in effectiveness (Z＝ 6.12,P＜ 0.01),and K score (Z＝ 3.31,P＜0.01).The effectiveness of the reatment group and the control group are 97.8％ and 73.4％ respectively.Conclusion The combined treatment resulted in an improved quality of effectiveness and Kamofsky scores.

With the progresses of the research on "Digital Human", more and more information has been needed for the setting up of the three-dimensional digital models of human organs. In the present paper, based on the method of block mapping with a normal-style bump mapping method, we normalized the vector of the surface of the models, computed offset of the texture coordinates and added to them. The projection of the coordinates to the tangential space complemented the disturbance to the vector of models' surface. The method was proposed with bump texture mapping on the surface of the biological models of organs to improve the impression of the visualization of the organ models and to enhance the sense of reality of the models.
Humans ; Models, Anatomic ; Models, Biological

Antiviral Agents ; therapeutic use ; Guanine ; analogs & derivatives ; therapeutic use ; Hepatitis B ; immunology ; Hepatitis B Antibodies ; immunology ; Hepatitis B Surface Antigens ; immunology ; Humans ; Reproducibility of Results ; Stem Cell Transplantation ; Stem Cells ; Tissue Donors

Objective To investigate the effects of Thomas solution in combination with nitroglycerine of different concentrations on rat cardiac preservation for 8 h. Methods Thirty Wistar rats were selected,and Langendorff heart perfusion models were established.Rats were randomly divided into Thomas solution group,Thomas solution+4?10-6 mol/L nitroglycerine group and Thomas solution+4?10-5 mol/L nitroglycerine group(n=10).After hypothermic preservation(4 ℃)for 8 h,the cardiac function of each group was determined.Tissues of left ventricular wall were obtained,and changes of myocardial ultrastructure were observed by electron microscopy. Results Compared with Thomas solution group,the cardiac function was significantly improved and the myocardial ultrastructure injury was less severe in Thomas solution+4?10-5 mol/L nitroglycerine group after hypothermic preservation(4 ℃)for 8 h,while there was no significant difference in myocardial ultrastructure injury and cardiac function between Thomas solution group and Thomas solution+4?10-6 mol/L nitroglycerine group. Conclusion Thomas solution in combination with 4?10-5 mol/L nitroglycerine can significantly improve the preservation effects for isolated hearts in rats.

Smoke water and distillation liquid were used to treat the seeds of Trichosathes kirilowii and to study the effects of smoke water and distillation liquid on the seed germination and seedling growth of T. kirilowii. The results showed that germination rate, germination index and germination vigor of T. kirilowii all were significantly improved with the treatment of SW and DL treatment. The activity of α-amylase were significantly increased with the treatment of SW and DL at 1:2,000. SW and DL treatment showed no significant effects on the activity of SOD. The activity of POD were markedly enhanced under the treatment of SW (1:000) and DL (1:2,000). CAT activity were increased with the treatment of SW and DL at 1:2,000 while were inhibited by SW and DL at 1:500. Seedling height and root length were increased with the treatment of SW and DL (1:1,000, 1:2,000). SW and DL treaments improved the content of chlorophyll, and moreover with the concentration of SW and DL, the stimulatory were also increased. This work demonstrated that smoke water and diatillation liquid at 1:2,000 could stimulate the seed germination and seedling growth of T. kirilowii, and it provided the references for the study of seed germination technology.
Agriculture ; instrumentation ; methods ; Distillation ; Germination ; Seedlings ; growth & development ; metabolism ; Seeds ; growth & development ; metabolism ; Smoke ; analysis ; Trichosanthes ; growth & development ; metabolism ; Water ; chemistry ; metabolism

Objective To analyze the clinical manifestations and possible gene mutation sites of Chinese patients in order to improve the clinician's understanding of CHARGE syndrome. Methods Clinical data were collected and blood samples were obtained from the proband of CHARGE syndrome and their relatives. The peripheral blood DNA was extracted and sequenced by PCR amplification. Mutation sites were verified by Sanger sequencing. Results For the first proband, a heterozygous mutation was detected in the intron 10 of CHD7 gene. His parents and brother did not have mutation. For the second proband, total repeat sequence in exon 7 of CHD7 gene was detected. His father carried the same mutation and his mother did not have mutation. Conclusion For the patients who are diagnosed with CHARGE syndrome based on the clinical manifestations, genetic mutation detection should be proceeded. It is useful for studying possible genetic pathogenesis and enhancing the awareness of clinicians.

Objective To examine the expression of scinderin in primary hepatocellular carcinoma (HCC) and its paired portal vein tumor thrombus (PVTT) tissues; and to explore its role in the development of HCC and its relationship with prognosis of HCC.Methods Immunohistochemistry was used to detect the expressions of scinderin in tumor-adjacent normal liver tissues,HCC tissues and PVTT tissues from 33 patients.Results The positive expression rates of scinderin in tumor-adjacent normal tissues,HCC tissues and PVTT tissues were 69.7％ (23/33),45.5％ (15/33) and 24.2％ (8/33) respectively.A significantly lower scinderin expression was found in HCC tissues when compared with tumor-adjacent normal tissues (P ＜ 0.05).Also,the expression of scinderin in PVTT tissues was significantly lower than in HCC tissues (P ＜ 0.05).The expression of scinderin in HCC tissues significantly correlated with tumor size,absence of tumor capsule and serum AFP (P ＜0.05).The recurrence-free survival (RFS) and overall survival (OS) of Scinderin-positive patients were significantly longer than Scinderin-negative patients (P ＜ 0.05).Multivariate analysis revealed scinderin expression level to be an independent risk factor affecting RFS and OS after curative resection.Conclusions Scinderin was down-regulated in HCC tissues and PVTT tissues when compared with its paired tumor-adjacent normal liver tissues.The expression level of scinderin correlated with HCC recurrence and prognosis.Scinderin can be used as an indicator of prognosis of HCC patients with PVTT.

Objective To explore the clinical significance of serum cystatin C (Cys C) combined with brain natriuretic peptide(BNP)in more earlier,more accurate diagnosis of contrast-induced nephropathy(CIN)after coro-nary angiography or percutaneous coronary intervention .Methods 358 patients undergone coronary angiography or percutaneous coronary intervention were enrolled in this study , and the data were collected .The serum creatinine (Scr),Cys C and BNP were tested before operation .The serum Cys C and BNP were tested 8h,16h,24h after opera-tion.The serum Scr was tested 24h,48h,72h after operation.All patients were diagnosed and grouped with CIN by ser-um Scr.The results were compared between the CIN group and control group ,diagnosis efficiency of Cys C ,BNP and combine with them was analyzed .Results CIN occurred in 16 cases of 358 enrolled patients , the incidence was 4.47%.The levels of Scr increased beyond 24h after operation in the CIN group .The levels of serum Cys C and BNP had no significant difference at 8h after operation compared to preoperation (P>0.05),and significantly rosed at 16h and 24h after operation(P<0.01)than before in the two group,[16h Cys C:(1.63 ±0.49)mg/L vs(0.91 ±0.33) mg/L,24h Cys C:(3.45 ±0.83)mg/L vs(0.91 ±0.33)mg/L,16h BNP:(215.96 ±55.72)ng/L vs(102.40 ± 32.23)ng/L,24h BNP:(352.41 ±61.08)ng/L vs(102.40 ±32.23)ng/L,all P<0.01].The levels of serum Cys C and BNP had no significant difference at 8h after operation in CIN(P>0.05),and significantly rosed at 16h and 24h after operation in CIN( P<0.01)than the corresponding levels in the control group [16h Cys C:(1.63 ±0.49)mg/L vs(0.93 ±0.38)mg/L,24h Cys C:(3.45 ±0.83)mg/L vs(0.92 ±0.34)mg/L,16h BNP:(215.96 ±55.72)ng/L vs(149.32 ±43.74)ng/L,24h BNP:(352.41 ±61.08)ng/L vs(164.82 ±49.62)ng/L,all P<0.01].The diagno-sis efficiency of serum Cys C combined with BNP at 16h after operation had high degree of accuracy .Conclusion In this study,the serum Cys C and BNP are better biomarker for early diagnose of CIN ,witch can diagnose CIN earlier than Scr .Combine of the two biomarker can more earlier ,more accurate diagnose CIN .

Objective To investigate the curative effect of the preoperative intervention transcatheter arterial chemoembolization and preoperative intravenous systemic simple chemotherapy for gastric cancer.Methods Retrospective analysis of 55 patients diag-nosed with gastric cancer.These patients were divided into two groups:treatment group and control group (simple chemotherapy group),with treatment group of 35 cases and control group of 20 cases,and cases of two groups all received surgical radical surgery for gastric carcinoma treatment.Preoperative and postoperative clinical symptoms were observed.Data of tumor sizes and tumor vessel changes were analyzed and compared.The difference of curative effect between two groups was observed.Results Compared with control group,degeneration and necrosis of tumor cells in gastric cancer tissue of treatment group were obvious,and tumor ves-sels significantly reduced,with nonuniformed,and became thinner.Number of tumor vessels of treatment group was obviously low-er than that of control group (P <0.05).Conclusion The interventional therapy of gastric cancer has obvious short-term therapeutic effects,and can reduce tumor vessels effectively and improve surgical resection rate.