Objective To investigate the effects of different concentrations of isoflurane on neuronal apoptosis in hippocampus and cerebral cortex in the aged rats. Methods Ninety female pathogen-free SD rats 22-24 months old weighing 497-593 g were randomly divided into 3 groups: group Ⅰ oxygen group inhaled air enriched with 40% O2,groupⅡ and Ⅲ were anesthetized with 1.2% and 1.8% isoflurane for 3 h respectively. Morris water maze was used to assess the cognitive function. The animals were killed at 24 and 72 h and on the 7th day after emergence from anesthesia and their brains were removed. Neuronal apoptosis in hippocampus and cerebral cortex was detected by TUNEL. Results The average escape latency was significantly longer on the 2nd and 3rd day after isoflurane anesthesia in isoflurane group than in oxygen group (P < 0.05 ). The neuronal apoptesis rate was significantly higher in the 2 isoflurane group than in oxygen group (P < 0.05 or 0.01 ). Conclusion Isoflurane anesthesia can result in transient cognitive dysfunction, and the mechanism may be involved in apoptosis in cerebral cortex in the aged rats.

Objective To evaluate the clinical value of selective salpingography(SSG)and fallopian tube recanalization(FTR) for diagnosis and treatment of oviduct obstructive infertility with self-made simple coaxial microtube.MethodsSSG and FTR with self-made simple Coaxial Microtube were performed for 76 cases with 134 tubes of obstructive oviduct through wire-guided tubal recanalization,anti-inflammatory and anti-adhesion effect of recanalization fluid,and the patients were treated with anti-bacterial and hydrotubation after operations.ResultsSSG displayed that 81 tubes obstruction in interstitial tubal,25 tubes obstruction in isthmus,16 tubes obstruction in ampulla and 12 tubes obstruction in fimbria.The total recanalization rate of FTR was 76.1%,with the recanalization rate 96.3% in interstitial,the recanalization rate 68.0% in isthmus and the recanalization rate 43.8% in ampulla,and no serious complications occurred.All cases were followed up for one to two years postoperatively.Normal pregnancy pationes were 31 cases(40.8%),and 29 women were normal parturition,2 cases(2.6%) were extrauterine pregnancy.ConclusionThe advantage of SSG and FTR with self-made simple coaxial microtube is low cost,simplicity and excellent efficacy.It is worth popularizing in grass-roots hospitals.

This article discusses the ethical problems which appear when medical laboratory technicians deal with all kinds of interpersonal relations in the field of medicine from the point of present medical service.It advocates that medical staff should possess professional morality and the sense of duty and calls for humane spirits to set up a brand-new mode of medical ethics.

Animals ; Autophagy ; Coronaviridae ; genetics ; pathogenicity ; physiology ; Coronaviridae Infections ; physiopathology ; virology ; Humans ; Virus Replication

Female ; Hepatitis C ; complications ; drug therapy ; Humans ; Interferon-alpha ; therapeutic use ; Interferons ; therapeutic use ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; complications ; drug therapy ; virology ; Middle Aged ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Treatment Outcome

Antiviral Agents ; therapeutic use ; Drug Combinations ; Hepatitis C, Chronic ; drug therapy ; psychology ; Humans ; Interferon-alpha ; therapeutic use ; Polyethylene Glycols ; therapeutic use ; Recombinant Proteins ; therapeutic use ; Ribavirin ; therapeutic use

Humans ; Periodontal Diseases ; therapy

Public Health Ophthalmology is a population-,region-or country-based as a whole object,and is committed to improving the eye health of the whole population.Epidemiological investigation of the eye was named to the region from a single disease to variation of diseases from cross-sectional survey to a longitudinal cohort study.Epidemiological investigations is the most important way which can reflect the pathogenic factors for public health policy-making,early diagnosis and prevention and treatment of eye disease,and Research to Prevent Blindness.Imaging Diagnosis,quality control,improve the reliability of the epidemiological investigation,and to strengthen the study of disease,race,and molecular levels and create a unique epidemiological study.

Objective: To investigate the effect of miR-143 (microRNA-143) on biological activity of pancreatic cancer PANC-1 cells. Methods: MiR-143 mimics were chemically synthesized and transfected transiently into PANC-1 cells by LipofectAMINE™ 2000. The expression of miR-143 in pancreatic cancer cells was examined by real-time fluorescence quantitative PCR. The cell proliferation was measured by MTT method. The apoptosis was detected by flow cytometry. Transwell and wound healing assays were performed to examine the migration ability of PANC-1 cells. The dual luciferase reporter vectors containing 3'-UTR (3'-untranslated region) with miR-143 binding site of wild type or mutant TAK 1 (transforming growth factor-β activated kinase 1) were constructed by using Dual-Luciferase® Reporter Assay System, and then the relative activity of Renilla reniformis luciferase was detected to confirm the binding site of miR-143 on TAK1's mRNA. Results: The expression of miR-143 in miR-143 mimicstransfected PANC-1 cells was significantly up-regulated. The transfection of miR-143 failed to influence the activities of proliferation and apoptosis of PANC-1 cells (P > 0.05), and however, the migration ability of PANC-1 cells was reduced (P < 0.05). Compared with negative control or wild type TAK 1 or mutant TAK 1, cotransfection with miR-143 and wild type TAK 1 3'-UTR could significantly decrease the relative activity of Renilla reniformis luciferase. Conclusion: miR-143 can restrain the migration ability of PANC-1 cells in vitro , in which TAK 1 may be one of the target genes. Copyright © 2012 by TUMOR.