Objective To observe the effect of slmvastatin of serun levels of matrix metalloprotease-9(MMP-9) and tissue inhibitors of matris metalloproteinase-1(TIMP-1) in patients with acute coronary syndromes(ACS).Methods There were 30 patients with stable angina pectoris(SAP) and 30 patients with ACS.28 cases of age matched healthy individuals were selected as a control.The serum level of MMP-9 and TIMP-1 were detected by ELISA before and after treatment.Results There was a significant diferencein serum level of MMP-9 and TIMP-1 among SAP group,ACS group and control group.The serum level of MMP-9 and TIMP-1 were diferent between simanastatin treatment before and after 4 weeks treatment.Conclusion ACS may be related to the increased serum level of MMP-9 and the decreased serum level of TIMP-1.Simavastatin can decrease serum level of MMP-9 and increase serum level of TIMP-1,and it may be have a beneficial effect on clinical treatment.

OBJECTIVE:To observe the clinical efficacy of famciclovir combined with compound glycyrrhizin in the treatment of herpes zoster.METHODS:158 patients with herpes zoster were randomly divided into treatment group and control group(n=79).Treatment group was treated with famciclovir and compound glycyrrhizin for 7 days,and control group was given oral dose of famciclovir for 7 days.RESULTS:The regression time of the symptoms and signs in treatment group was significantly shorter than in control group (P0.05).CONCLUSION:Famciclovir combined with compound glycyrrhizin is superior to famciclovir alone in the treatment of herpes zoster.

In order to know the feature and the difficulty level of auditory comprehension of sentences in the aphasics,the Boston Diagnostic Aphasia Examination was used to exam 72 aphasics. The difficulty level of & yes-no questions in 72 aphasics' examination was analysed.

Personalized therapy has became a main method to break through efficacy of chemotherapy in lung cancer. At present, we have got a few progressions in personalized therapy, but which are very limited. This review discourses upon the dilemma and hope of individualized treatment from the following ways:limitation of based on histology type to determine treatment regimens, undecided sequential therapy after failure of TKI as front-line therapy for advanced NSCLC patients with EGFR mutation, clinical study of maintenance therapy and EGFR mutation detection based on peripheral blood being trap or surrogate for tumor tissue to direct personalized TKI therapy.

BACKGROUND: Fms-like tyrosine kinase-3 (FLT3) gene is one of the receptors for growth factors in early hematogenesis. By binding to its ligand (FL), FLT3 regulates the proliferation and differentiation of hematopoietic stem/pregenitor cells via cell signal transduction pathway. FLT3 juxtamembrane internal tandem duplication (ITD)mutation is correlated with leukemia onset, development and act as an independent factor for poor prognosis.OBJECTIVE: To explore the relationship between Flt-3/ITD mutation and acute leukemia (AL), and analyze its clinical implication.DESIGN: A repetitive measurement.SETTING: Department of Biochemistry and Molecular Biology, Shenyang Medical College.PARTICIPANTS: Totally 147 samples of leukemia patients were diagnosed by morphological bone marrow biopsy in Shenyang Central Hospitals affiliated to Shenyang Medical College from January 1999 to October 2004. They were 95 males and 52 females with the mean age of 14.5 years old (5-23 years old). According to FAB standard, there were 62 patients with acute leukemia [including 33 cases of acute myelocytic leukemia (AMLs, M1, M2), 3 cases of acute myelo-monocytic leukemia (AMMOLs, M4), 18 cases of acute monocytic leukemia (AMOLs, M5), 8 cases of acute megakaryocytic leukemia (AMKLs, M7)], 43 patients with acute lymphoblastic leukemia (ALLs), 13 patietns with juvenile chronic myelocytic leukemia (JCMLs) and 29 patients with myelodysplastic syndrome (MDSs). Informed contents were obtained from all the participants.METHOD: Flt-3/ITD was detected with polymerase chain reaction (PCR) sequence in the 147 leukemia patients.MArN OUTCOME MEASURES: The clinical manifestations and prognosis were observed in the ALL patients with Flt-3/ITD;The Flt-3 gene expression, Flt-3/ITD positive rate, duplication region and length were detected in all kinds of leukemia.to Flt-3 gene products, including 3 cases of AMLs (M1, M2), 1 case of AMMOL (M4), and 1 case of AMOL (M5). DNA sequencing and blast alignment revealed that ITDs existed in all samples with unusual products within exon 11, in various region and length (33-72 bp). Expression of Flt-3 in different levels was found in 43 cases of ALL, 29 cases of Flt-3/ITD all died short after the diagnosis, and the mean life span of was only 10.8 months.

Objective To establish dried blood spot determination for quantitative analysis of creatine phosphokinase (CK) activity; To analyze the correlation of CK value between serum detection and dried spot determination, and set up a 'cut-off point' for screening of pathologically high CK values in dried blood spot determination. MethodsA total of 100 samples were analyzed quantitatively with two methods (serum detection and dried blood spot determination) for CK activity. The correlation of the two methods was studied. The ' cut-off point' for screening of pathologically high CK in dried blood spot determination was calculated with receiver operating characteristic curve (ROC curve). ResultsOf the 100 samples, 50 were normal and other 50 were abnormal when the 'cut-off point' was set at serum CK of 170 IU/L. There was a positive correlation in CK values between serum detection and dried blood spot determination (r = 0.764, P < 0.01). However, the values measured with the two methods could not be interchanged. The sensitivity and the specificity were 90% when the 'cut-off point' was set at 150 lUlL. The sensitivity and the specificity were 70% and 72%, respectively, when the 'cut-off point' was set at 400 IU/L. ConclusionsThere was a good correlation in CK activity between serum detection and dried blood spot determination. 400 IU/L could be suggested as the 'cut-off point' for detecting pathologically high CK in dried blood spot determination.

Objective To establish renal calculus rat model induced by calcifying nanoparticles .Methods We extracted and cultivated calcifying nanoparticles from clinically diagnosed patients with nephrolithiasis .Forty adult male SD rats were divided into 4 groups (n=10 each):group A(given an intravenous injection of calcifying nanoparticles) ,group B(given an intravenous injection of calcifying nanoparticles and taken Shenshitong granules) ,group C(given an intravenous injection of calcifying nanoparticles and intraperitoneal injection of gallium nitrate) ,group D (as normal control) .Eight weeks later ,all rats were sacrificed .The kidneys were examined for pathology .Results Histopathological studies revealed calcifying nanoparticles induced renal tubular epithelial cells fatty degeneration and renal tubular crystallization ,and caused renal interstitial infiltration with inflammatory cells .The forma‐tion of renal crystals in group A was more obvious than those in group B and C(P<0 .05) .In group D ,no crystals were formed and no pathological changes occurred .The levels of urinary calcium and urinary creatinine(of All group) at one day before the observa‐tion period (T2 ) were higher than those at 4 weeks after injection(T1 )(P<0 .01) .The levels of urinary calcium and serum creati‐nine in group A and B and C were significantly higher than those in group D at T2 (P<0 .05) .The levels of urinary calcium in group B and C were lower than that in group A at T2 (P<0 .05) .However ,there were no apparent differences of the levels of serum calci‐um and urinary creatinine between groups at T2 (P>0 .05) .Conclusion Calcifying nanoparticles could cause renal tubular epithelial cells injury ,which leads to the formation of kidney stones .

In order to evaluate the diagnostic value of determing the serum strotium levelfor the drowning diagnosis,we carried out this study.The results of our expe-rirnental study are summarized as follows:(1).The blood Sr level determinationcould be used as a confirmatory test of drowning merely at the early stage(wi-thin 5 min)of drowning;(2).It could give some help for diagnosing the case ofdrowning submerged in water within 24 hours.It was showed that the durationof submersion has influence upon the postmortem blood Sr level.The blood Srlevel was measured in two submerged cases in medico-legal practice.One died ofdrowning and the another was postmortem sbmersion after electrocusion.It is inter-esting that the results of blood Sr determination of human being were more acc-urate than experimental animals.Therefore,we consider that the further studyabout this project is required.