11 plant pathogenic strains were isolated from the crown gall of Japanese cherry (Prunus xyroloensis)in Japanese cherry growing areas, such as Chixing. fenhua and Shenzhou counties of Zhejiang province. Ten ofthem were identified as Agrobacterium tumefaciens biotype 1 based on their morphological. physiological andbiochemical characterizations and bacterial protein SDS-PAGE test. The plate antagonistic assay and the potbiocontrol test indicated that the isolated strains of A tumefaciens biotype 1 were sensitive to the well-knownantagonistic bacterium , K84 strain. The K84 strain could inhabit the tumor production by the pathogenic satins.

Accompanying with the development of technological level,optical coherence tomography plays a more and more important role in predicting the surgical outcome for idiopathic macular hole.Preoperative morphologic parameters like minimum diameter,base diameter,maximum height,macular hole index,hole form factor,tractional hole index,macular hole closure index,photoreceptor inner-outer segment junction,cone outer segment tips line,central subfield retinal thickness and macular hole inner opening diameter were proved correlated to postoperative visual acuity and anatomical results.It has important application value to know these predictors to judge the surgical outcome and deepen understanding of idiopathic macular hole.

Objective To investigate the effect of celecoxib on endometrial carcinoma cell HEC-1-B and expression of the COX-2 mRNA.Methods To investigate the inhibition of different concentration Cele- coxib on the growth of human endometrial carcinoma cell HEC-1-B by the method of cell culture and MTT experiments.Detect the changes of expression of the COX-2 mRNA by RT-PCR.Results Celecoxib could effectively inhibit the growth of the HEC-1-B cell in dose and time dependent manner.Celecoxib could inhib- it the expression of the COX-2 mRNA.The inhibition ratio of expression in COX-2 mRNA of 20?mol/L and 50?mol/L Celecoxib is 25.92 % and 50.81% respectively.Conclusion Celecoxib may inhibit the growth of endometrial carcinoma cell by degrading the expression of the COX-2 mRNA.

Methyltransferase is an important metabolic enzyme whose main function is to catalyze the methylation of nitrogen, oxygen and sulfur atoms. It plays an important role in the metabolism of exogenous and exogenous compounds, including drugs in vivo. Methyltransferases are widely distributed in different tissues, with the liver and kidneys being the most abundant. In addition, the structure and activity of the enzyme have certain species and individual differences. This article will describe the biological properties of methyltransferases and their role in drug metabolism.

OBJECTIVETo explore clinical efficacy and key matters for the treatment of femoral intertrochanteric fracture and integrity of lateral trochanteric wall by proximal frmoral nail antirotation (PFNA).

METHODSFrom June 2010 to December 2012,210 femoral intertrochanteric fracture patients treated with PFNA were retrospectively analyzed, including 76 males and 134 females aged from 46 to 96 years old with an average of 71 years old. All fracture were caused by injury and classified to type I (5 cases) type II (16 cases), type III (73 cases) and type IV (116 cases) according to Evans classification. The time of getting out of bed, postoperative complications and displacement of screw blade and fracture healing were observed, Baumgaertner criteria were used to evaluate quality of fracture reduction, Harris criteria were used to evaulate hip joint function.

RESULTSAll incisions were healed at stage I, no complications occurred except incomplete of lateral trochanteric wall patients without reconstruction, other patients could get out of bed with crutches at one week and all patients discharged from hospital at 10 days after operation. One hundred and seventy-eight patients were followed up from 3 to 17 months with an average of 10 months. One case occurred unhealed fracture displacement caused by screw blade cutting, 2 cases occurred screw blade transfomed to proximal and out femoral head, other patients obtained fracture healing at 12 to 16 weeks after operation. According to Baumgaertner criteria, 130 cases obtained good results, 45 cases acceptable, and 3 poor; while 107 cases obtained excellent results, 65 good, 3 good and 3 poor according to Harris score.

CONCLUSIONPFNA with mechanical advantage of intramedullary fixation has advantsges of stable fixation, shorter operation time, minimally invasive. Satisfied clinical effects could obtained by grasping fixation principle, dealing with negative factors in operation. Intraoperative reconstruction for integrity of lateral trochanteric wall could assure stable fixation and earlier get out of bed.

Aged ; Aged, 80 and over ; Bone Nails ; Female ; Femoral Fractures ; surgery ; Fracture Fixation, Intramedullary ; instrumentation ; methods ; Hip Fractures ; surgery ; Humans ; Male ; Middle Aged ; Retrospective Studies

Objective To investigate the etiological factors and treatment of thoraco-gastric perforation (TGP) after esophagogastrostomy. Methods Retrospective analysis was carried out in 16 patients with thoraco-gastric perforation after esophagogastrostomy in our department from March 1974 to March 2004. The etiological factor, clinical feature, experiment test and the method of treatment were compared between TGP and patients of thoracic esophageal anastomosis leak (TEAL). Results Thoraco-gastric perforation occurs within 2～5 days postoperatively. Among these 16 cases, in 8, local necrosis of gastric wall was found which was caused by severe contusion and massive ligature. In 5, were penetrative injury of gastric wall caused by suture needle. In 3,unsuitable purse-string suturing of corner of greater or lisser gastric curvature. Hydropneumothorax occurred after thoraco-gastric perforation. Chest fluid was brown and turbid with putrefactive odor. With medium examination, anastomosis was normal, but medium and air bubbles were found outside of the stomach. Perforation were repaired and covered by pedicle tissue-flap in all cases. 15 cases were cured with no sequence. Conclusion TGP after esophagectomy were correlated with technique of surgery. TGP often occurs early after esophagogastrostomy. Transthoracic repairing of TGP should be done as soon as possible. The healing ratio of reparation was about 93.8%.

The effects of ketotifen, cimetidine & erythromycin on thebphy-lline pharmacokinetics in rabbits were studied. The results suggested that the elimination half-life of theophylline decresed from 2.74 ? 0.75 to 2.33 ? 0.56 h & the clearance of theophylline increased from 0.15?0.036 to 0.13 ? 0.047 1/kg?h-1 by ketotifen.The elimination half-life of theophylline increased from 2 .08 ? 0.33 to 2.6l?0.45 h & the clearance decreased from 0.2l?0.03 to 0.16?0.03 1/kg?h-1 by cimetidine.The elimination half-life of theophylline increased from 2.39 ? 0.43 to 3.54?0.89 h & the clearance decreased from 0.15?0.03 to 0.12?0.03 1/kg?h-1 by erythromycin.

History and current situation of hospital preparation in our country and practical demands of pharmaceutical industry were analyzed. With the implementation of GMP in pharmaceutical industry and enhanced drug administration, lots of problems were exposed. The scale of hospital preparation was decreasing year by year, though it still stayed at a predominate position in hospital works currently.

To improve the quality of the pediatric education in clinical clerkship and to bring us more in line with international practice,we applied the PBL bedside teaching in the clini-cal clerkship of the pediatric education.Compared with the traditional bedside teaching,better re-sults could be obtained with improvement in the consciousness of study,communication skill,the combination of basic and clinical knowledge,and the establishment of the clinical thought and evi-dence-based thought.

With the increasing frequency of international exchanges and cooperations,students from foreign countries,such as India and Pakistan,have come to our country for their higher degree education.We studied retrospectively and explored the teaching methods of Histology in English,and further provide our thoughts and viewpoints on improving the teaching qualities of foreign students.