Objective To explore the surgical treatment of intra -articular calcaneal fractures with bone nodules joint angle in the preoperative and intraoperative evaluation of prognosis of postoperative analysis.Methods 22 cases of affected joint surface after calcaneal fractures,open reduction and internal fixation with steel plate in the operation treatment.Used Sanders classification:preoperative,intraoperative,postoperative again calcaneal tubercle joint angle measurement.According to the Maryland foot scoring system to evaluate postoperative effect.Results All patients were followed up for 8 to 20 months.The excellent and good rate of the patients was 90%,the postoperative complications:heel broadening,subtalar arthritis in 2 cases,poor healing in 2 cases (after the treatment and healing). Conclusion It has high value of the calcaneal tubercle joint angle measurement in the joint surface after calcaneal fractures.

ObjectiveTo establish a comprehensive evaluation method on sensitivity of Yersinia pestis to different antibiotics, then use the method to evaluate the sensitivity of Yersinia pestis to 6 antibiotics. Methods Through literature review, related articles were consulted and sensitivity testing data to antibiotics were collected,and the sensitivity of Yersinia pestis to the 6 antibiotics was assessed with analytic hierarchy process. The sensitivity of Yersinia pestis to the 6 antibiotics(Cefotaxime penicillin, Ampicillin, Cefazolin, Ciprofloxacin, Ciprofloxacin Buddha's, and Streptomycin) was evaluated comprehensively with analytic hierarchy process from the antibacterial situation of the antibiotics to Yersinia pestis of 141 strains of virulent, attenuated EV76paris strains and isolates of the bacteria of Yunnan. Results The evaluation showed that the sensitivity of Yersinia pestis to antibiotics was different. The rank of the sensitivity of Yersinia pestis to the 6 antibiotics was: Ceftazidime, Ampicillin, Cefazolin,Ciprofloxacin, Norfloxacin and Streptomycin. Composite score index were 1.730 77, 1.631 77, 1.581 95, 1.567 80,1.449 48, and 0.999 99, respectively. Conclusions Analytic hierarchy process evaluation can be a reasonable,objective and more accurate evaluation on the sensitivity of Yersinia pestis to different antibiotics. This method could provide some references for screening feasible pharmaceuticals against plague.

Objective To study the change of kidney injury biochemical index before and after SWL in southeast of Chongqing, and toanalyze the constituents of urinary stones,thus to provide the evidence for the treatment of patients with urinary stones by SWL in southeast of Chongqing.Methods 328 patients with urinary stones form our hospital in Southeast of Chongqing during 2008 to 2014 underwent SWL were enrolled.And we detected the levels of urine mALB,serum β2-MG,BUN,Cr before and after operation.The results were statistically analyzed.The chemical constituents of stones were analyzed.Results The levels of urine mALB,serumβ2-MG obviously increased on day 1-2 after operation,and reached the peak 2 days after operation.The result after 1-2 days showed significant difference(P <0.01).The levels returned to normal after 10 days.Compared with the levels before operation,the results after 10 days showed no significant difference(P >0.05).Compared with the levels before operation,the levels of BUN and Cr slightly increased,with no significant difference(P >0.05).Conclusion The detection of urine mALB,serum β2-MG of patients with urinary stones before and after SWL and analysis of the chemical compositions of urinary stones could provide the evidence for the treatment of patients with urinary stones by SWL in Southeast of Chongqing.

Objective To observe the effects of 8-week aerobic exercise on skeletal muscle glycogen content and exercise endurance and investigate the possible mechanism in rats with chronic heart failure. Methods 30 Sprague-Dawley rats were submitted to heart failure sedentary (H) group, heart failure traning (HT) group and sham operation (S) group. The heart failure model was established with coronary artery ligation. HT group performed an 8-week treadmill running. The skeletal muscle glycogen content was determined with anthracenone, exercise endurance with exhaust duration of graded exhausted exercise test, glucose uptake rate, activity of glycogen synthase (GS) and glycogen phosphorylase (GP) with radicisotope, protein expression of total GS, phospho-GS (p-GS), total GP and phospho-GP (p-GP) with Western Blotting. Correlation analysis was conducted between glycogen content and exhaust duration. Results The skeletal muscle glycogen, exhaust duration, glucose uptake rate, GS activity and p-GP protein reduced (P<0.05), while serum insulin, GP activity and p-GS protein raised (P<0.05) in the H group compared with those in the S group. The skeletal muscle glycogen, exhaust duration, glucose uptake rate, GS activity and p-GP protein increased (P<0.05), while serum insulin, GP activity and p-GS protein decreased (P<0.05) in the HT group compared with those in the H group. There was positive correlation between glycogen content and exhaust duration (P<0.05). Conclusion Prolonged aerobic exercise enhanced skeletal muscle glycogen content and exercise endurance in rats with chronic heart failure, which may be related to the improvement of insulin sensitivity, muscle glucose uptake, increase of glycogen synthesis and decrease of glycogen breakdown.

Sleep deprivation (SD) has been taken as an independent predictor for cardiovascular risks, which was closely related to the increased morbidity and mortality in coronary heart disease (CHD). In this article, after reviewing the impact of modern medical method sleep deprivation on CHD and studies on principle method recipe medicines for preventing and treating CHD, the authors observed the autonomic nerve dysfunction, hormonal metabolism dysfunction, endothelial dysfunction and inflammatory responses after sleep deprivation, which can cause or aggravate CHD. On the basis of the traditional Chinese medicine theories of "heart dominating the blood and vessels and the mind", the authors considered that traditional Chinese medicines can tonify heart and soothe the nerves, reducing all of the risk factors through multi-target and multi-pathway, and improve sleep and decrease the risk factors caused by sleep deprivation, which provides a new idea for the prevention and treatment of CHD.
Animals ; Coronary Disease ; drug therapy ; etiology ; prevention & control ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Sleep Deprivation ; complications

Anemia, Iron-Deficiency ; blood ; diagnosis ; Biomarkers ; blood ; Child ; Child, Preschool ; China ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Infant ; Male ; Receptors, Transferrin ; blood

Objective To compare the efficacy of different antibiotics strategy,introperitoneal (IP) cefazolin plus third-generation cephalosporin versus IP Vancomycin plus thirdgeneration cephalosporin on peritoneal dialysis (PD)-related peritonitis.Methods All episodes of PD -associated peritonitis happened in prevalent PD patients between January 2008 and December 2012 were recruited from the PD Center of Peking University First Hospital.According to their empiric antibiotics scheme,episodes were divided into group A (where IP cefazolin plus third-generation cephalosporins were administrated) and group B (where IP Vancomycin plus third-generation cephalosporins were administrated).Multivariable logistic regression model was used to explore the influence of different emnpiric antibiotics scheme on peritonitis outcome.Results Patients in Group B had significantly lower level of serum albumin (33.5±6.0 vs 35.3±5.2 g/L) and cholesterol (4.6±1.3 vs 4.9± 1.1 mmol/L) than those in group A.In group A,the percentage of gram-positive bacteria was similar to group B (43.2％ vs 43.3％,P =0.96),but gram-negative bacteria was numerically lower (16.9％ vs 24.7％,P =0.08).Different empiric antibiotics strategy was not independent predictor of peritonitis outcome [OR =1.07,95％ CI(0.45,2.56),P=0.87].Conclusion Both cefazolin and vancomycin can be selected as first-line empiric antibiotic covering gram-positive organisms in the treatment of PD related peritonitis.

Objective To investigate the correlation between tetramine poisoning and hypocalcemia in children.Methods According to different severe cases grade,tetramine poisoning children were divided into critical group(70-90 score),extremely critical group(

Objective To investigate the effect of erythropoietin (EPO) on the expression of integrin-β1 in hippocampus of neonatal rats after hypoxic-ischemic brain damage (HIBD) and to discuss the neuroprotective mechanisms for EPO.Methods Neonatal Sprague-Dawley rats of 7 days old were randomly divided into 3 groups by random number table method (24 cases in each group):sham-operated group,HIBD group and EPO treated group(EPO group),then each group was further divided into 4 subgroups (n=6) based on different time points following the injection of 9 g/L or EPO (6 h,24 h,3 d,7 d).The expressions of integrin-β1 and integrin-β1 gene in hippocampus were determined by Western blot and real-time fluorescent quantitative PCR(RT-qPCR).Results By Western blot method:integrin-β1 had low expression in the hippocampus of the sham-operated group at each time point,which had no significant difference(0.47±0.22,0.45±0.18,0.54±0.24,0.53±0.22,F=.223,P=0.879).The expression of integrin-β1 in HIBD group was increased at first and then decreased,which had a significant difference(0.62±0.16,1.42±0.32,1.13±0.37,0.81±0.21,F=11.809,P=0.000).In the EPO group the expression of integrin-β1 was rising significantly at 24 h and 7 d and showed two peaks (0.64±0.21,1.10±0.37,0.96±0.30,1.19±0.31,F=3.741,P=0.028).Compared with the sham-operated group at corresponding time points,the integrin-β1 protein expression showed significant increase at 24 h and on 3 d in HIBD group and 24 h,3 d,and 7 d in EPO group (all P<0.05).By RT-qPCR:there was no significant difference in the gene expression of integrin-β1 in ippocampus of the sham-operated group at different time points (0.31±0.13,0.33±0.16,0.34±0.15,0.32±0.17,F=0.036,P=0.990).Gene expression of integrin-β1 in HIBD group was increased at first and then decreased and the summit was at 24 h point(0.72±0.35,1.39±0.33,0.95±0.24,0.61±0.25).The difference between each time point was statistically significant (F=8.022,P=0.001).Integrin-β1 gene expression of EPO group showed two peaks at 24 h and on 7 d(0.77±0.30,1.09±0.27,0.90±0.38,1.17±0.36),and there was a significant difference between 6 h group and 7 d group (P<0.05).Compared with the sham-operated group at corresponding time points,the expression of integrin-β1 gene in between HIBD group and EPO group was extremely higher(all P<0.05).Besides,the gene expression of integrin-β1 in EPO group was higher than that in the HIBD group on 7 d with statistically significant difference (P<0.05).Conclusion Erythropoietin can upregulate the expression of integrin-β1 in the recovery phase of HIBD,which may be one of the beneficial effects of EPO for HIBD.

Objective To Observe the influence of Zaoshi Jiedu Zhupi Sanjing (ZPSJ) Recipe on insulin sensitivity, serum resistin and inflammatory factors related to the effects of Chinese herbal compound in type 2 diabetic patients with insulin resistance. Methods A single-blind randomized controlled clinical trial was conducted. 80 patients with 2 type diabetic patients with insulin resistance were recruited into a control group and a treatment group. The treatment group was treated with ZPSJ Recipe, and the control group was treated with metformin. Results In each group before and after treatment: blood glucose, serum insulin levels, HOMA-IR were significantly decreased (t=7.14, P<0.01 ) ; between the two groups, FPG, FINS showed no significant difference, while TG TCH, HOMA-LR showed significantly differences (t=2.62, P <0.05 ). Within the two groups before and after the treatment: serum IL-6, TNF-a, hs-CRP were decreased in treatment group, (t= 1.85, P<0.05 ) , while TNF-a, hs-CRP were decreased in the control group (t=2.56, P<0.05 ) , while IL-6 did not change significantly. The serum resistin levels were not statistically changed in both groups before and after treatment. Conclusion ZPSJ Recipe can regulate blood sugar, blood fat and improve insulin sensitivity in type 2 diabetic patients with insulin resistance.