Objective To explore the clinical significance of FPSA/PSA ratio in the differential diagnosis of prostate carcinoma(Pca) and benign prostate hyperplasia (BPH). Methods The concentrations of FPSA and PSA in 52 patients with Pca or BPH were analysed retrospectively to calculate FPSA/PSA ratio. Results FPSA/PSA ratio between the Pca and BPH was markedly different (t=4 01,P

Medical imaging teaching depends on support from multimedia database which is constructed with image information provided by PACS/HIS ( Picture archiving and communication system/Hospital information system ).This paper evaluated construction,administration and application of the multimedia database,and illustrated the importance of PACS/HIS in construction of the database,as well as of the database in medical imaging teaching.

Adult ; Cardiomyopathy, Hypertrophic ; complications ; pathology ; Humans ; Male ; Myocardium ; pathology

Myasthenia gravis (MG) is an immune-mediated disorder of the neuromuscular junction that is characterized by fluctuating muscle weakness.Acetylcholine receptor antibody (AchR-Ab) is the first MG specific antibody being elucidated,however,the discovery of many other MG specific or related antibodies enriches its immunopathogenesis greatly.The detection of antibodies is very important for the diagnosis and classification of MG.It can be used to guide the clinical management and evaluate the response to treatment as well.With the development of laboratory medicine,the methods of antibody detection are also being optimized.In this paper,we will review the serum antibodies closely associated with MG and their mechanisms,detection methods and clinical significance.

Streptozotocin-induced diabetic rats were treated with GSH for 12 weeks.The results showed that GSH significantly improved the expressions of NF-KB and inducible nitrie oxide synthase and ameliorated the myocardial tissue injury.

A high active soybean isoflavone glucoside hydrolase-producing mould strain was isolated from spirit qu. Its optimal hydrolase-producing conditions were as follows: 2.5% wheat bran as carbon source, 1% NaNO3 as nitrogen source, initial pH7. 0, culture medium volume 40mL/250mL, inoculating quantity 8% , culture temperature 30℃, revolutions 160r/min and culture time 84h. The enzyme activity reached 82 U/mL. Cu2+ can inhibit Absidia sp. R strain from producing the hydrolase, the influence of other metal ions was not remarkable on it.

OBJECTIVETo determine the content of linarin in Yuye Jiedu granule.

METHODA HPLC method was developed. The chromatographic conditions are as follows Luna C18 column and acetonitrile-0.05 mol x L(-1) phosphate buffer-phosphoric acid (30:70:0.06) as mobil phase, detection wavelenth at 327 nm.

RESULTThe linear range of linarin was 0.025-0.50 microg. The average recovery was 98.7% and RSD 2.7%.

CONCLUSIONThe method is simple and accurate, with good repeatability, and can be used for determination of linarin in Yuye Jiedu granule.

Chromatography, High Pressure Liquid ; methods ; Chrysanthemum ; chemistry ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; chemistry ; isolation & purification ; Glycosides ; analysis ; Lonicera ; chemistry ; Plants, Medicinal ; chemistry ; Quality Control

Aged ; Humans ; Lower Extremity ; Middle Aged ; Muscle, Skeletal/physiology* ; Vibration

Background: Biotherapy based on human bone marrow?derived mesenchymal stem cells (BMSCs) is currently the focus of research, especially in the field of autologous stem cell transplantation. A novel type of metastasis?associated magnetic resonance (MR) molecular imaging probe was constructed, and the changes in metastasis and proliferation of hepatocellular carcinoma (HCC) before and after BMSC intervention were observed through MR imaging (MRI). Methods:Metastasis?associatedMRmolecularimagingprobe,integrin αvβ3 ligandcRGD?PEG?DGL?DTPA?Gd (Gd?RGD),wereconstructed. After human BMSC intervention was performed for 6weeks, tumor weight inhibition rates were calculated, and the RGD molecular probe was imaged through MRI with molecular imaging agent Gd?DTPAas control.The signal?to?noise ratio (SNR) and contrast?to?noise ratio (CNR) in the MRI experiment were used as semi?quantitative indicators. Polymerase chain reaction method was performed to detect proliferation? and metastasis?associated indicators, transforming growth factor β?1 (TGFβ1), osteopontin (OPN), and integrin subunit αv and β3. Results: The highest tumor weight inhibition rates were observed 3 weeks after the BMSC transplantation. The MR Gd?RGD in the HCC tissues after the BMSC intervention showed less enhancement than Gd?DTPA. The Gd?DTPAMRI of control group had higher SNR and CNR than Gd?RGD MRI in the experimental groups (P < 0.05). For high metastatic potential hepatocellular carcinoma (MHCC97?H), significant differenceswereobservedintheSNRsandCNRsofGd?RGDMRIbeforeandaftertheBMSCintervention(P<0.05).Forlowmetastaticpotential hepatocellular carcinoma (MHCC97?L), the CNRs of Gd?RGD MRI were statistically different before and after BMSC intervention (P < 0.05). With regard to MHCC97?H, OPN, β3, and TGFβ1 expression significantly decreased after BMSC intervention (P < 0.05). In MHCC97?L and OPN, β3, TGFβ1, and αv expression after BMSC intervention decreased, and the difference was statistically significant (P < 0.05). Conclusions: The CNR index of MRI is a good indicator for distinguishing high? and low?metastatic potential HCC tissues.After BMSC transplantation of MRI through the two kinds of tracer, the SNR and CNR indexes can distinguish two kinds of high and low metastatic potential HCC tissues, and Gd?RGD imaging is more suitable in distinguishing the metastatic potential changes through BMSC intervention.

r study.