Objective:To study the effect of secretin on subdiaphragmatic vagal afferent response by gastric distension. Methods: Experiments were performed on anesthetized rats. The sensory impulses of subdiaphragmatic vagal nerve were recorded using a window discriminator. Through a midline abdominal incision, a polyethylene cannula (1.0mm ID) for duodenal infusion was inserted into the proximal duodenum 0.5mm distal to the pylorus.Another cannula was placed into jejunum 20 cm distal to the ligament of Treitz.Gastric distension was produced by injecting saline at 37℃ into a small balloon via a polyvinyl tube.Results:Intravenous injection of secretin in a dose of 5.0 pmol/kg/h inhibited significantly the response of afferent discharges induced by gastric distension( n=7, P 0.05). It showed that exogenous secretin in a dose of 5.0pmol/kg/h, in the physiological dose range, played an inhibitory effect. 0.03N HCl was infused intraduodenally at 9 ml/h for 20 min in anesthetized rats with gastric cannulas, ligation of the pylorus, respectively.0.1 ml of a rabbit antisecretin serum (titer=l:l,000,000) was injected intravenously in a bolus 20～40min before HC1 was administered. During the duodenal infusion of 0.03N HCI the gastric distension-vagal afferent responses were suppressed significantly.( n=8, P

Objective To investigate the clinical effect of mifepristone combined with misoprostol on the pla-centa adherence.Methods 80 cases with placenta adherence were randomly selected and divided into control group and treatment group.The treatment group was treated with mifepristone and misoprostol,while the control group was given oxytocin by intravenous injection.In addition,patients of two groups were orally given Shenghua Tang once dai-ly.The vaginal bleeding of all the patients were measured within 12h,24h and 72h after the parturition.In addition, the time when the placenta was expelled out of the uterus was also recorded.After the clinical treatment,standardized protocols were closely followed for all patients to record the vaginal bleeding.B -mode ultrasound was used to observe the uterine reversion and determine whether there were intrauterine residues.Results The vaginal bleeding of the two groups had no significant difference within 12h and 24h.But after 72h,the vaginal bleeding of the treatment group was less(t =2.436,P <0.05).What's more,it take 3 -7 days to expel the placenta out of the uterus in the treatment group,while 11 -13 days in the control group.And the cure rate of the control group was only 77.5%,but that of the treatment group was increased to 95.0%(χ2 =5.165,P <0.05).Conclusion The combination of mifepristone and misoprostol has therapeutic efficacy to the placenta adherence,which is worthy of clinic application.

Objective To analyze the psychosocial influential factors on job burnout and their interaction models in first aid medical staff.Method A survey was carried out concerning job burnout status,job stress,job overload,work-family conflict,overall job satisfaction,coping styles and social support in 504 first aid medical staffs,by which to seek the psychosocial influential factors of job burnout status and explore their interaction models using stepwise multivariable regression and path analysis.Result (1) The stepwise multivariable regression on the job burnout showed that job overload,work stress,work-family conflict,overall work satisfaction and negative coping style were the key social-psychological influential factors in job burnout(F =90.562,P =0.001).(2) The path analysis showed that the job overload was the major event caused the work stress,and work stress played a direct and indirect roles in the level of job burnout in first aid medical staff,and the work-family conflict and overall work satisfaction was disposition factors of job burnout with direct actions and indirect actions.The negative coping style was an intermedium link between work stress and job burnout.Conclusion Decrease of work intensity and change of bad coping styles can be effective on job burnout in first aid medical staff.

Objective To survey the incidence of depression in Parkinson disease(PDD) and explore the related factors of PDD.Methods 127 patients with Parkinson disease were investigated,all of them were assessed by Hamilton Depression Rating Scale,self-designed register form and the Unified Parkinson disease scale(UPDRS).A stepwise multiple factor regression analysis was carried out to determine factors that best accounted for the occurrence of PDD.Results The overall incidence rate was 47.2%.The minor,moderate and major incidence was 14.2%,28.3% and 4.7% respectively.The regression analysis showed that factors accounted for the occurrence of PDD were gender,the severity of PD,stage of patient's motor impairment,education level and the use of dopamine agonists.Conclusion PDD seems to have a multifactorial etiology.Knowledge of risk factors related to the occurrence of PDD may help guide neurologists in the early prediction and treatment of this disease.

Animals ; Bilirubin ; toxicity ; Brain ; drug effects ; metabolism ; Nitric Oxide ; metabolism ; Rabbits

AIM: To study whether the excitatory amino acids (EAAs)-triggered excitotoxicity contribute to the evolution of hyperbilirubinemia-associated brain injury. METHODS: Newborn rabbits with hyperbilirubinemia were decapitated and then, Na~+-K~+-ATPase activities, neurotransmitters and non-neurotransmitters concentration in brains were determined. RESULTS: It was found that the activities of Na~+-K~+-ATPase both in brains and cytomembrane and the amounts of glutamate (P

Objective To explore the effects of automobil exhaust on immune adherence function of erythrocytes in mice Methods The male Kunming mice were exposed to gasoline fueled engine exhausts 20 minutes per day continouusly for 20 days After the whole exposure,the ratio of spleen to body weight and total count of erythrocytes in peripheral blood of mice were observed The immune adherence function of erythrocytes was determined by complement sensitized yeast cell hemagglutination test in mice Results The ratio of spleen to body weight [(4.84?0.53)mg/g] and total count of erythrocytes [(5 83?10 12?0 25?10 12)/L] of mice exposed to automobil exhausts were significantly lower than those of control group [(5 55?0 68)mg/kg,(7 71?10 12?0 29?10 12)/L] respectively The positive rate of blood coagulation of exposure group (12 5%) was significantly lower than that of control group (82 5%) at blood coagulation titer of 1∶8,? 2=39 2,P

Acute Disease ; Cytogenetics ; Humans ; Leukemia ; genetics

BACKGROUND:White spot lesions caused by enamel demineralization are a common side effect of orthodontic treatment. Resin infiltration treatment for white spot lesions has obtained favorable effects, but there is no report on the difference between resin infiltration and fluoride varnish treatment for post-orthodontic white spot lesions. OBJECTIVE:To compare the efficiency of resin infiltration and fluride varnish for treatment of post-orthodontic white spot lesions. METHODS:Twenty-nine patients with post-orthodontic white spot lesions were randomly divided into two groups:one group accepted the resin infiltration treatment and the other group accepted fluoride varnish treatment. Frontal intraoral photos before treatment and 6 months after treatment were col ected for study. The difference in efficiency of resin infiltration and fluride varnish for treatment of post-orthodontic white spot lesion was analyzed and evaluated by both dentists and College students who were not engaged in oral medicine. RESULTS AND CONCLUSION:Except one patient treated with fluride varnish, al the patients completed the referral on schedule. There was significant improvement in the two groups after treatment (P<0.05). Resin infiltration treatment was better than fluoride varnish treatment in post-orthodontic white spot lesions. Thus, resin infiltration that requires fewer referrals and has better effects is an ideal therapy for post-orthodontic white spot lesions.

Objective To explore the feasibility, difficulties and indications of video-assisted laparoscopic pancreaticoduodenectomy. Methods Clinical records of 5 cases of laparoscopic pancreaticoduodenectomy in this hospital from November 22, 2002 to April 28, 2005 were analyzed, with respect to preoperative diagnosis, surgical methods and procedures, intraoperative blood loss, operation time, difficulties encountered and countermeasures, and postoperative recovery. Related parameters included patient's temperature, drainage volume, blood routine, liver functions, amylopsin changes, postoperative complications, bowel movement recovery, and length of hospital stay. Results All the 5 cases of operation were successfully performed, with an operation time of 360~660 min (mean, 528 min) and an intraoperative blood loss of 150~2 000 ml (mean, 770 ml). Pathological examinations revealed 3 cases of well-differentiated papillary adenocarcinoma of the duodenum, 1 case of endocrine small-cell carcinoma of the head of pancreas, and 1 case of papillary adenomatoid hyperplasia of the duodenum with malignant changes accompanying intermediated-differentiated adenocarcinoma of upper and middle intrahepatic biliary duct. Postoperatively, a small amount of pancreatic leakage occurred in case number 1 and a digestive tract stress ulcer happened in case number 2, all of which were cured by symptomatic management. In case number 4, a recurrent pancreatitis with pulmonary infection was observed, and the patient died from stress ulcer hemorrhage on the 39th postoperative day. Except for the case number 4 in which the patient's highest temperature reached 39.5 ℃ after the recurrence of pancreatitis, the highest temperatures of the other 4 patients were all