B-Lymphocytes ; Graft vs Host Disease ; Hematopoietic Stem Cell Transplantation ; Humans

Animals ; Toxins, Biological ; chemistry ; physiology

Objective To evaluate modified Soave procedure for the treatment of Hirschsprung's disease for the neonate and infant. Methods Seventeen cases of short and sigmoid types of Hirschsprung's disease were treated by transanal modified Soave procedure. The incision was made 1cm posterioly and 2-3 cm anterioly above the dental line; the dissection progressed from the submucosa layer up to the perineal reflex into the pelvic cavity, and the rectum was pulled through. "V" shap muscular cuff was excised on the posterior wall. The proximal colon was anastomosed to the anal mucosa. Results Mean operative time was 160 min ? 45 min, mean blood loss was 45 ml ?35 ml. No enterocolitis, anastomotic leak, perianal infection and anastomotic stenosis occurred. All patients recovered with normal defecation, and no complication was observed from a follow-up of more than 4 mos. Conclusion Modified Soave procedure is safe and effective for the treatment of Hirschsprung's disease in the neonate and infant.

Objective To investigate the signal transduction of bFGF to regulate the colorectal carci- noma cell growth via PI3K/PKB pathway.Methods MTT assay was used to determine the suppressive effect of bFGF on the growth rate of LoVo cell;(?-~(32)P)ATP incorporation assay was used to detect the increased activity of PKB in LoVo cell;the RT-PCR technique was used to test the expression of cyclin A;Western Blot analysis was used to detect the expressions of PKB and cyclin A protein.Results When the bFGF worked on the LoVo cell at various times,it was found that the PKB activity of the LoVo cell was increased by the(?-~(32)P) ATP incorporation assay.By preprocessing by the depressor-LY294002 and bFGF,the activity of PKB was sig- nificantly reduced(P

BACKGROUND:The current studies concerning the effect of obesity on children are limited to metabolic physiology, and there is little evidence available on skeletal muscle and motor ability.
OBJECTIVE:To compare isokinetic knee muscle strength between obese children and normal children, and analyze the causes of physical performance decline in obese children.
METHODS:A total of 56 children were recruited in this study, including 28 obese children and 28 normal children. The isokinetic muscle strength was tested by CON-TREX. The gaits were tested by VICON. The statistical analysis of al the differences between obese children and normal children were measured using SPSS 19.0 software.
RESULTS AND CONCLUSION:At the same gait speed, obese children had higher absolute peak torque and average power than normal children (P<0.05), but relative peak torque and relative average power showed no significant difference compared with normal children (P>0.05) except extensor group at 60(°)/s was significantly lower than normal children. At the same gait speed, the torque and power of extensor group were significantly higher than that of flexor group in the two groups (P<0.05);he obese children showed significant differences in the absolute peak torque and absolute average power compared with relative peak torque and relative average power of flexor group at 120(°)/s (P<0.05). In the two groups, the extensor exhibited higher torque in high speed, while the high flexor torque was found in low speed. Normal children had faster cadence and walking speed, significantly smal er step width and shorter stride time than obese children (P<0.05). Obese children have smal er relative strength and higher absolute strength than normal children;in addition, the low cadence and walking speed are found in obese children. These factors contribute to weak limb strength and insufficient motor ability.

Purpose:To identify clinical predictors of stage ⅢA-N2 in patients with non-small cell lung cancer. Methods:From March 1995 to February 1998, 118 patients with pathological stage ⅢA-N2 non-small cell lung cancer who underwent a resection by Shanghai Chest Hospital were analysed. Prognostic factors were estimated from the date of operation using the Kaplan-Meier and log-rank analysis. The Cox regression model evaluated the influence of factors on the survival. Results:The overall of 3-year and 5-year survivals of the 118 patients were 40.5% and 18.4%. Margin, the number of N2 lymph nodes and stations, the cycles of adjuvant chemotherapy were associated with survival. In a multivariate analysis, the number of N2 lymph nodes and the cycles of adjuvant chemotherapy significantly influenced survival. Conclusions:The number of N2 lymph nodes and the cycles of adjuvant chemotherapy were important prognostic factors of stage ⅢA-N2 non-small cell lung cancer.

Objective To study the best parameters of low dose MSCT in examining the pharyngeal disease.Methods 20 volunteers were scanned by MSCT using 10 mm collimation,10 mm interval,120 kV,200 mA,100 mA,50 mA,25 mA parameters,respectively.All of the data was reformed(collimation 2.5 mm,interval 1.3 mm) on ADW 4.0 workstation.The image quality in different dose were scored respectively by 4 experienced radiolagists. Results The best appropriate parameter was 10 mm collimation,10 mm interval,120 kV,50 mA.Conclusion In examining the pharyngeal disease,the application of low dose(120 kV,50 mA) scan mode not only can assure the image quality but can decrease the exposure to patients.

Objective To evaluate the diagnostic value of double-balloon push enteroscopy in patients with obscure small bowel bleeding. Methods Thirty-four cases with suspected small bowel bleeding were received double-balloon push enleroscopy from April to November 2003. Results The lesions were detected in 30 of 34 cases; the total diagnostic yield was 88. 3% . The enteroscopy reached to middle jejunum, middle or low portion of ileum in 1 and 3 cases with negative findings. Among thirty positive cases, the diagnosis were angiodysplasia 7 cases (20.6% , 1 in jejunum, 3 in jejunal-ileum, 3 in ileum) , small bowel tumor 11 cases (32.4% ) , all cases were certified by operation and pathology. The tumor growth in duodenum, jejunum and ileum were 2, 5 and 4 cases respectively. Pathology of incised tumors included lipoma, leiomyoma hemangioma, pheochromoeytoma, Kaposiform hemangioendothelioma and adenoearcinoma, 4 cases with Crohn's disease (11. 8% ) , and 8 cases in others. Conclusion The most common causes of obscure small bowel bleeding were angiodysplasia, small bowel tumor and Crohn's disease. Double-balloon push enleroscopy was a safe, reliable and effective diagnostic modality with high clinical value for obscure small bowel bleeding.

Objective To discuss the opportunity of hemilateral craniotomy in treatment of a large area combined middle cerebral artery (MCA) infarction.Methods Thirty-two patients with a large area combined MCA infarction were performed by hemilateral craniotomy.Initial clinical presentation was evaluated by the Glasgow coma scale(GCS).All survivors were assessed three months after surgical decompression according to Barthel index(BI).The effects of pre-hemia decompressive surgery(before any signs of cerebral hernia,based on clinical status and CT or DWI findings)versus post-hernia surgery (after signs of hernia) on mortality,functional outcome.Results In 18 patients with pre-hernia decompressive surgery,3 patients (16.7%)were dead and average BI was (66.94±7.75)scores.The mortality was 57.1%(8/14)and average BI was (38.43±9.82)scores of post-hernia surgery with 3 cases severe aphasia.Conclusion Hemilateral craniotamy is performed before occurrence of cerebral hernia may decrease the mortality and improve outcome in these patients.