With the rapid development of high science and technology,new teaching methods related to computer and electronic message techniques,have been applied to the recent medical education.Classical pathological teaching model has been gradually replaced by the modern way.This paper reports our teaching reformed practice by using the new technology and form the current educational theory.

Objective To evaluate the recent effects of IMRT plus gemcitabine for locally advanced non-small-cell lung cancer.Methods The data of forty-five patients were retrospectively reviewed.The treatment group consisted of 23 cases,treated with sequential same chemotherapy regimens and IMRT,the control group consisted of 22 cases,receiving same chemotherapy regimens after two cycles of gemcitabine and cisplatin induction chemotherapy.Resuls The remission rates were 65.2 ％ (15/23) and 31.8 ％ (7/22) respectively in treatment and control group.The 1 year survival rates were 66.4 ％ and 45.0 ％ respectively in treatment and control group.The remission rates and 1 year survival rates were significant different between two groups (both P ＜ 0.05).Conclusion The short-term effect of sequential chemotherapy and IMRT is better than chemotherapy and the adverse events can be better tolerated.

This paper reviewed the existing literatures of executive function in obsessive -compulsive disorder (OCD) patients, and critically examined four neurocognitive tests in the recent ten years' studies, including stroop task, Wisconsin Card Sorting Test(WCST),London tower, and Trail-making test. In addition, we discuss the problems occurred in the past experiments, and put forward suggestions for further studies.

Objective To compare the efficacy between the brucea javanica oil emulsion combined with the whole radiotherapy group (treatment group) and only radiotherapy group (control group) in non-small cell lung cancer patients with brain metastases.Methods The clinical data of 53 non-small cell lung cancer patients with brain metastasis were retrospectively reviewed,including 27 cases in treatment group and 26 cases in control group.Results The remission rates were 77.8 ％ (21/27) and 69.2 ％ (18/26) respectively in treatment group and control group.The improvement rates of Kamofsky in the treatment group and control group were 66.7 ％ (18/27) and 30.8 ％ (8/26),the stable rates were 22.2 ％ (6/27) and 30.8 ％ (8/26),the decrease rates were 11.1％ (3/27) and 38.5 ％ (10/26).The remission rates of clinical sympotoms were 92.6 ％ (25/27) and 69.2 ％ (18/26) respectively in treatment group and control group.The difference of remission rate between two groups has no statistic significance (P ＞ 0.05),while the difference of the quality of life and clinical sympotoms between two groups were significant respectively (P ＜ 0.05).Conclusion The brucea javanica oil emulsion combined with the whole radiotherapy can relieve clinical sympotoms and improve the quality of life of patients,and may play a role on improving the effect of radiotherapy.

Objective To establish and observe the effects of CD46-siRNA on proliferation,in-vasion and metastasis of MCF-7 human breast cancer cell.Methods The pEGFP-C1-CD46 siRNA was established and transfected into the MCF-7 cell.Changes in CD46 mRNA and protein expression were test-ed and variation of invasion and migration capacity in MCF-7 /CD46-siRNA cell was also observed.Results Compared with the control group and scrambled group,the expression of CD46 mRNA was significantly decreased(P <0.05)and CD46 protein expression was also suppressed in the MCF-7 cells of the silence group.Proliferation activity of MCF-7 cell in the control group and scrambled group was gradually in-creased with the time,but this phenomenon was not obvious in the silence group.At the 3th and 4th day af-ter transcription,differences in proliferation activity were significant among the groups,and activity in the silent group was significantly lower than the other two groups(P <0.05).Counts in invasion and migration of MCF-7 cell in the control group and scrambled group were significantly higher than the silent group (P <0.05 ).Conclusion The expression vector of pEGFP-C1-CD46 siRNA can suppress the CD46 mRNA and protein expression in MCF-7 human breast cancer cell and reduce the capacity in proliferation activity,invasion and migration.

Objective To evaluate the clinical efficacy and safety of modified anti -tuberculosis scheme in the treatment of tuberculous meningitis.Methods 60 cases with tuberculous meningitis were randomly divided into two groups.We enrolled 30 patients in each group.The traditional anti -tuberculosis group used traditional anti -tuberculosis scheme with isoniazid,rifampicin,pyrazinamide,streptomycin,and the modified anti -tuberculosis group was given isoniazid,rifampicin,pyrazinamide,levofloxacin,the treatment course of tuberculous meningitis was 12 months.The efficacy and safety through clinical manifestations and laboratory examinations,the dynamic changes of cerebrospinal fluid were evaluated by statistical methods.Results The total effective rate of the modified anti -tuberculosis group was significantly higher than the traditional anti -tuberculosis group(86.6% vs.63.3%,χ2 =4.36,P <0.05).The cerebrospinal fluid routine and biochemical index in the modified anti -tuberculosis group were obviously superior to the traditional anti -tuberculosis group after treatment(all P <0.05).There was no significant difference in the incidence of adverse reactions between the two groups(26.6% vs.16.7%,χ2 =0.88,P >0.05).Conclusion Levofloxacin combined with other first -line anti -tuberculosis drugs,glucocorticoids,dehydration and other comprehensive measures improved brain function for the treatment of tuberculous meningitis is recommended.

Diagnosis, Differential ; Female ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Leukemia, Myeloid, Chronic-Phase ; genetics ; Lymphoma ; classification ; diagnosis ; pathology ; Male ; Neoplasms ; genetics ; metabolism ; pathology ; Oncogene Proteins ; metabolism ; Prognosis ; Prostatic Neoplasms ; genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Translocation, Genetic ; Tumor Suppressor Proteins ; metabolism

Objective The objective of this study is to investigate the psychological stress status of Chinese college students ,and to provide evidence for mental health education in colleges and universities .Methods A random sampling method was conducted to perform the questionnaire (psychological stress scale for college students by Zhang Lin ,Che Wenbo ,etc .) on psychological stress a‐mong 205 college students .Results The main sources of psychological stress on college students were job‐seeking pressure ,school pressure ,academic pressure ,emotion pressure and interpersonal pressure .The scores on college environment and learning task stress of engineering and technology students were significantly higher than other divisions (P< 0 .05) .Male students showed markedly higher stress level than female students in love pressure ,inferiority pressure and academic pressure (P< 0 .05) .The scores on frustration pressure and family pressure of students with siblings were significantly higher than students from one‐child family (P<0 .05) .The scores on family pressure of rural students were significantly higher than those from towns and cities (P<0 .01) .Conclusion The psychological stress of college students in Hainan province are overall in good condition .There are some differences among different groups .We should pay more attention to college students′mental health problems ,and make separate counseling according to the profession and family background difference .

Objective To observe the changes of c-fos protein expression in brain and beta-endorphin (β-EP) level in blood plasma in rats with diffuse brain injury (DBI) and secondary brain insult (SBI) after intraperitoneal injection of naloxone hydrochloride, and explore the role of c-fos and β-EP in development of SBI in rats. Methods Seventy health male SD rats were enrolled in the present study and randomly divided into group A (intraperitoneally injected with 0.9% saline after DBI and SBI model was reproduced), group B (injected intraperitoneally with 1.0mg/kg naloxone hydrochloride after DBI and SBI model was reproduced), and group C (intraperitoneally injected with 1.0mg/kg naloxone hydrochloride after DBI and before SBI model was reproduced). The animals were sacrificed 3, 24 and 48 hours after injury, and the number of c-fos positive cells in brain and content of β-EP in blood plasma were determined by immunohistochemistry and radioimmunoassay respectively, the water content and number of injured neurons in brain tissue were measured by pathomorphological observation of the brain tissue. Results No significant difference was observed between group B and C for all the detection parameters. In group B and C, the water content in brain tissue at 3h and 24h was found to be decreased, while the number of injured neurons at 24h and 48h increased, number of c-fos positive cells in brain at 3h, 24h and 48h decreased, and content of β-EP in blood plasma at 3h and 24h decreased when compared with group A(P<0.05). Conclusion Naloxone hydrochloride could decrease the c-fos expression in brain and β-EP level in blood plasma, alleviate the nerve injury, and protect neural function. The therapeutic effect of naloxone administered either after DBI and SBI or after DBI and before SBI was similar.

Head and Neck Neoplasms ; radiotherapy ; Humans ; Radiotherapy, Intensity-Modulated ; methods ; Salivary Glands