Objective To investigate the misoprostol and calcium combined treatment of postpartum hemorrhage of cesarean section clinical curative effect.Methods The subjects were cesarean section 68 cases of pregnant women,which were randomly divided into the observation group and the control group,34 cases in each group,the control group at 600 micrograms misoprostol,observed in this group based on the use of calcium(before skin incision slow intravenous push 50％ GS 20 ml + 10％ calcium gluconate 10 ml),observe two groups of intraoperative,postpartum 2 hours and 24 hours postpartum hemorrhage volume.Results In the observation group,intraoperative blood loss[(188.47 + 13.29) vs(385.24±14.16 ml)]was significantly less than that of the control group,2 hours postpartum bleeding volume[(133.56±11.45) vs(219.4±12.73) ml]was significantly less than that of the control group,24 hours postpartum bleeding volume[(171.29±21.91) vs(257.36±33.42) ml]was also significantly less than that of the control group,the difference was statistically significant(t =1.982,1.895,1.714,all P ＜0.05).Conclusion Misoprostol and calcium combined treatment can significantly reduce postpartum hemorrhage of casarean section on maternal and child safety,security,worthy of clinical application.

The present study used optical coherence tomography (OCT) to observe the relationship between inhibitor of monocyte chemoattractant protein-1 (MCP-1) and coronary in-stent restenosis after stenting ,and proved that MCP-1 is a key inflammatory factor causing in-stent restenosis after stenting .

Colorectal cancer is a common malignant tumor in digestive system ,with an increasing inci-dence rate and case fatality rate all over the world in recent decades .At present,chemotherapy still plays a very important role in the treatment of advanced colorectal cancer .Targeted therapy brings new hope to patients ,and new chemotherapy drugs and targeted drugs have been widely used in clinics .Patient′s life quality has been great-ly improved,survival time has been significantly prolonged ,the combination of chemotherapy and targeted therapy has also become a hot research area in the treatment of metastatic colorectal cancer .XELOX ( capecitabine plus oxaliplatin)as a first-line chemotherapy regimen in treating metastatic colorectal cancer (mCRC)can obtain good therapeutic effect ,the side effects of XELOX are light and well tolerated;Bevacizumab ,as a new type of targeted anti-tumor drugs ,shows a good effect in inhibiting tumor growth and reducing its hematogenous spread risk .Bev-acizumab in combination with XELOX regimen for treating mCRC has been widely investigated and reported .This review gives a brief summary on the efficacy and safety of the combined administration of Bevacizumab and XE -LOX.

BACKGROUND:Both acupuncture and neural stem cel therapy have good achievements in the treatment of cerebral palsy, but there are few reports about their combined therapy. OBJECTIVE:To observe the therapeutic effect of combined therapy of acupuncture and neural stem cel transplantation on cerebral palsy rats and to explore the relevant mechanism of action. METHODS: Cerebral palsy models were built in newborn rats, and then, the model rats were subjected to acupuncture, neural stem cel transplantation and their combination, respectively. Meanwhile, normal control group and model group were set up. RESULTS AND CONCLUSION:Compared with the other groups, in the combined therapy group, the behavior scores of cerebral palsy rats improved significantly (P < 0.05), the number of nerve cels and Bcl-2 protein expression were increased significantly (P < 0.05), and the number of apoptotic nerve cels and Bax protein expression were decreased significantly (P < 0.05). Additionaly, there were no significant differences in these indicators among the model, acupuncture and neural stem cel transplantation groups. These findings indicate that the combined therapy of acupuncture and neural stem cel therapy can obviously improve learning and memory ability of cerebral palsy rats and reduce neuronal apoptosis, which is probably associated with down-regulation of Bax protein and up-regulation of Bcl-2 protein.

BACKGROUND:Acupuncture has been shown to impact cerebral blood flow, relieve cerebral edema, improve microcirculation and reduce neural cel apoptosis in animals with cerebral palsy.
OBJECTIVE:To further verify the effect of acupuncture on neuronal apoptosis in cerebral palsy rats.
METHODS:Forty-five Sprague-Dawley rats were randomly divided into sham group, model group and the acupuncture group, with 15 rats in each group. In the model and acupuncture groups, rat models of cerebral palsy were established by ligating the left common carotid artery. In the sham surgerygroup, the left common carotid artery was not ligated. Rats in the acupuncture group received acupuncture from 2 days after model induction, once a day, for 20 consecutive days. Rats in the sham surgery and model groups were left intact. At 21 days aftersurgery, neurological functions, pathomorphological changes and neuronal apoptosis were observed.
RESULTS AND CONCLUSION:(1) Motor function score was significantly lower in the model group than in the sham surgery group (P< 0.05). Motor function scorewas significantly higher in the acupuncture group than in the model group at 21 days (P< 0.05). (2) Hematoxylin-eosin staining: In the model group, cels were scattered. Inflammatory cel infiltration and cystis degeneration were found. The number of nerve cels was reduced. Degeneration, necrosis, gliosis and karyopyknosis were detected. Cavitation was visible in some cels. Cel bodies became smal. The structure was not distinct or disappeared. In the sham surgery group, the morphology and structure ofnerve cels were normal. In the acupuncture group, the changes in morphology and structure of nerve cels were found between the sham surgery and model groups. (3) The number of apoptotic cels was significantly greater in the model group than in the sham surgery group (P< 0.05). The number of apoptotic nerve cels was significantly higher in the acupuncture group than in the sham surgery group (P< 0.05), but significantly lower than in the model group (P< 0.05). (4) Results confirmed that acupuncture therapy can improve neurological function, reduce nerve cel apoptosis, and play a protective effect on nerve cels of rats with cerebral palsy.

To treat 30 patients with simple adiposis with needling of point Jiaji (Ex-B2, T3-L5), after 2 courses of treatment 12 got a marked curative effect, 15 got a curative effect, 3 got no effect, accounting for 10.0%. The total effective rate was 90.0%.

Objective:Use ligustrazin and salvia miltiorrhiza to treat idiopathic pulmonary fibrosis,for the experimental result was perfect in animal modal.Method:These two drugs combined with prednisone in 15 patients as treated group,prednisone in 10 patients as control group.Result:At 1,6 months followed up,the dyspnea and cough improved markedly.Chest radiograph and pulmonary function improved slightly.There were no differences between two groups.After 24 month the combined group 13(73%) stabilized,4(27%) relapse or deterioration,no death.Control group 6(60%) stabilized,3(30%) relapse or deterioration,1(10%) death,Conclusion:Combined group was better then control group.Ligustrazin and salvia miltorrhiza could decrease alveolitis and inhibit the formation of procollagen.It also improve the patient's immunity.

Objective To evaluate the results of the Secuplate anterior cervical osteosynthesis plate (SACOP) for the treatment of patient with cervical spondylotic myelopathy (CSM). Methods Twenty six patients with CSM underwent anterior decompression fusion and internal fixation with SACOP were reviewed. All patients were followed up for 6 to 12 months (mean, 8 months). Results Neurological deficits improved in 23 of 26 patients after operation. A stable bony fusion according to radiological criteria was achieved in all cases 6 months postoperatively. There was no implants breakage and loosening. Conclusion Intervertebral fusion and internal fixation with SACOP after anterior decompression can offer immediate stability, prevent graft dislodgement, avoid cast immobilization and give a high rate of successful bony fusion for the patient with CSM.

To elucidate whether the mutations in X region of hepatitis B virus (HBV) might be responsible for the different clinical profiles in cases positive for antibody to hepatitis B e antigen. The nucleotide sequences of X gene regions in serum HBV were examined in 14 asymptomatic carriers (AsC) and 14 chronic active hepatitis (CAH) patients with antibody to hepatitis e antigen. The results showed that 12 of 14 AsCs (85.7%) had insertions, deletions or point mutations in nucleotide sequence of X region resulting in truncation of the X protein by creating frame shift mutation or a new stop codon, whereas no patient with CAH had those X gene mutations( P

Objective In order to further study the influence of a mutant on viral replication and transfection, a eukaryotic vector with mutation of 20/21 bp deletion (1748/ 1747 to nt 1767) in core promoter region and precore stop mutation (nt.1896) was constructed. Methods A linearized genome containing the entire HBV 3.5kb mRNA transcriptional units (P3.8Ⅰ vector) and initiating from the basic core promoter upstream sequences was used as a tool, the objective eukaryotic vectors were constructed by the molecular cloning and PCR based site directed mutagenesis in vitro. The capability of progeny virus production and transcription were examined with Southern blot and Northern blot analysis respectively, after transfection of the recombinant HBV plasmids into HepG2 cells by using liposome. Results The eukaryotic vectors were constructed successfully and their sequences were confirmed by clone sequencing. Both Southern and Northern blotting of DNA and RNA extracted from the transfected cells showed markedly reduced mutant activity to produce progeny virus, to transcript both 3.5kb precore/pregenome mRNA and 2.1kb preS/S mRNA. Conclusions The levels of replication and transcription are markedly reduced in the mutant compared with those in wild type HBV.