To analyze the acupuncture treatment for impotence in 130 kinds of classical medical books by computer,and summarize the rules of acupuncture for this disease.The results showed that selection of acupoints along the meridians,selection of acupoints by boay parts,selection ofacupoints by pattern identification are the laws ofacupuncture for this disease.

Objective To evaluate the association between serum ferritin and the risk of cardiovascular disease among the Chinese population.Methods Based on the data from China Health and Nutrition Survey 2009, we used the Framingham risk score to calculate the 10-year risk of cardiovascular disease and analyzed the association between serum ferritin and the risk of cardiovascular disease in different gender by logistic regression analysis.Results Among 6 551 respondents, 3 035 (46.3%) were males and 3 516 ( 53.7%) were females whose mean age were ( 49.3 ±13.8 ) and ( 49.2 ±13.7 ) years, respectively.The median level of serum ferritin was 75.08 ( 36.71 -138.75 )μg/L.According to the 10-year risk of cardiovascular disease calculated by the Framingham risk score , the low risk group, middle risk group and high risk group were accounted for 79.5%( n=5 207 ) , 15.7% ( n=1 028 ) and 4.8%( n=316), respectively.The median of serum ferritin (121.61 vs.46.14, P<0.01) and the Framingham risk score (8.6 ±6.0 vs.7.3 ±1.6, P<0.01) of males were higher than those of females.The percentages of the middle risk group ( 30.6% vs.2.8%) and high risk group ( 9.8% vs.0.5%) of 10-year risk of cardiovascular disease in males were higher than those in females ( P <0.01 ).The 10-year risk of cardiovascular disease ≥10%increased with the increasing of serum ferritin in females ( trend P<0.01 ) but not in males.Conclusion Serum ferritin levels are positively correlated with the risk of cardiovascular disease in female Chinese.

Animals ; Erythrocytes ; physiology ; Oncorhynchus mykiss ; physiology ; Oxygen ; Water ; chemistry

Animals ; Breast Neoplasms ; metabolism ; Humans ; NF-kappa B ; metabolism ; Neoplasms ; metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Receptors, Notch ; metabolism ; physiology ; Signal Transduction

Objective To evaluate the value of clinical findings and different diagnostic criteria for the diagnosis of adult onset Still's disease (AOSD). Methods The AOSD patients admitted to Zhongshan Hospital affiliated to Fudan University from 2003 to 2009 were enrolled. Non-AOSD patients with fever were selected. Different diagnostic criteria of AOSD were applied to all patients. Two indenpendent samples t test and wilco-xon test were used for statistical analysis. Results The clinical features such as rash, arthralgia, arthroncus, sore throat, myalgia, lymph node enlargement, hepatomegaly, splenomegaly, leuko-cytosis, neutrophil ≥80% and serum level of ferrin≥ 2000 ng/ml hadhigher specificity (65.87%~98.41%) with 2.00～5.00 of the positive likelihood ratio.High fever ( ≥39.0℃), negative of ANA and RF had higher sensi-tivity (85.25%~93.65%). Combinations of three parameters such as high fever, rash, sore throat, leukocy-tosis, arthralgia had higher positive likelihood ratio. The specificity of ARA criteria was the highest. The sensitivity and accuracy of Yamaguchi criteria were the highest. Conclusion There is no single parameter that could be specific to the identification of AOSD. Combing with several parameters can improve the diagnostic efficiency. The results of this study have shown that the commonly used diagnostic criteria has high specificity.

Chemical constituents of the essential oil in above-ground and root of Bupleurum malconense and root of B. chinense were investigated by GC-MS compiled with automated mass spectral deconvolution and identification system (AMDIS) and retention index. The results showd that the components of essential oil in B. malconense have some similarities with the one in B. chinense, and both of them have the higher content of caryophyllene oxide which is an active component of anti-inflammatory and analgesic. These results suggested that as a local substitute, B. malconense has a certain scientific basis of the treatment for cold fever.
Bupleurum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; therapeutic use ; Fever ; drug therapy ; Mass Spectrometry ; Oils, Volatile ; chemistry ; Plant Roots ; chemistry

OBJECTIVE: To establish a staging standard for a transplanted hepatoma model in rats, which may be equivalent to the human primary liver cancer. METHODS: A transplanted hepatoma model was made in SD rats by implantation of histologically intact Walker-256 tumor fragment into the left lateral lobe of the liver. The body weight, tumor volume and serum albumin level of the rats were measured and assayed in different periods. Pathological sections were also fabricated to detect the invasion and metastasis of the neighboring tissues. RESULTS: The body weight loss and ascites occurred at the 12th and 18th day separately. The serum albumin level was (35.32+/-2.45) g/L at the 11th day, which showed no statistical difference as compared with the healthy rats (P>0.05), and the albumin level at the 18th day was significantly lower than that at the 11th day (P<0.05). At the 23rd day, 56.25% of the rats developed bloody ascites. CONCLUSION: The period between 5-11 days in the model rats may be correspondent to the early stage of human primary liver cancer, and the biological behavior of the model rats at the18th day may be correspondent to the late stage of human primary liver cancer. This staging standard might be the guideline for model selection in experimental study of primary liver cancer.

This paper makes a statistical analysis on the acupuncture treatment of dementia recorded in 93 ancient books. The statistics show that the common acupoints are Shenmen ( HT 7), Shaoshang ( LU 11 ),Dazhong ( KI4), Yinbai ( SP 1 ), Houxi ( SI 3 ), Xinshu (BL 15), Yongquan (KI 1), Jiuwei (CV 15) and Baihui (GV 20); the common meridians are the Heart Meridian, Bladder Meridian and Kidney Meridian; the common areas are palm, medial side of leg, upper back and head; the acupoints of the heart meridian are frequently used, especially those in the distal end;heavy moxibustion functions to open orifices and needling to regulate qi.

Objective The K-ras gene plays a major role in the development , progression, and drug selection for the target treatment of colorectal cancer .The aim of this study was to evaluate the feasibility of detecting K-ras mutation in colorectal cancer by high-resolution melting ( HRM) analysis and to investigate the relationship between K-ras mutation and the clinicopathological parame-ters of colorectal cancer. Methods We collected the tissue samples of colorectal cancer from 179 patients, detected the mutations in codons 12 and 13 of the K-ras gene, and analyzed the relationship between K-ras mutation and the clinicopathological parameters of the patients. Results In the 179 cases of colorectal cancer, K-ras mutation was found in 77 (43.02%), significantly higher in those aged ≥60 years than in those aged <60 years (55.17% vs 33.70%, P<0.05).Multivariate logistic regression analysis showed a significant influence of age on K-ras mutation (OR=1.506, 95%CI:1.028-2.011, P<0.05). Conclusion HRM analysis is a rapid, sensitive, and inexpensive diagnostic tool for the detection of K-ras mutation, and K-ras mutation is associated with the age of colorectal cancer patients .