Objective To investigate the effects of the main harmful components in cigarette smoke on the expression of lung cancer susceptibility genes by use of the method of network pharmacology, and to explore the correlations of multiple targets and multiple components and diseases.Methods Literatures about the 11 main tobacco toxic ingredients of cigarette smoke were collected from PubMed and the multicomponent-genes-disease network was structured, and then, shared genes which affect the expression of lung cancer susceptibility were screened out.Then use Cytoscape software to construct the multicomponent-shared genes-disease network.Results Network analysis showed that 11 main harmful components in cigarette smoke influnce 106 lung cancer susceptibility genes, 57 lung cancer susceptibility genes of which were affected by at least 2 of the 11 components.Conclusion From the genetic point of view, the relationship of cigarette smoking and lung cancer was elucidated, and the effect of 11 components on the susceptibility genes of other diseases was also explored.This study may provide some statistical references for further detailed research targeting the relationship of cigarette toxic components and lung cancer genetic susceptibility.

Adenocarcinoma, Mucinous ; pathology ; Carcinoma, Pancreatic Ductal ; pathology ; Humans ; Prognosis

Adenocarcinoma ; diagnosis ; Cervical Intraepithelial Neoplasia ; diagnosis ; Diagnosis, Differential ; Female ; Humans

Cervical Intraepithelial Neoplasia ; diagnosis ; metabolism ; pathology ; Cervix Uteri ; metabolism ; pathology ; Cyclin-Dependent Kinase Inhibitor p16 ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Ki-67 Antigen ; metabolism ; Membrane Proteins ; metabolism ; Neoplasm Invasiveness ; Uterine Cervical Dysplasia ; diagnosis ; metabolism ; pathology ; Uterine Cervical Neoplasms ; diagnosis ; metabolism ; pathology

Inflammation is a common ocular surface disease.Glucocorticoid drugs are effective on the ocular surface inflammation,but their long-term and massive application is prone to serious side effects.Nonsteroidal antiinflammatory drugs (NSAIDs) have anti-inflammatory,anti-allergic,analgesic effects.The topical application of NSAIDs for the prevention and treatment of ocular inflammatory disease is much safer than that of glucocorticoid.Therefore,NSAIDs have more and more concerns in the treatment of ocular surface inflammation in recent years.Although NSAID has good anti-inflammatory effectiveness and less adverse effects,it should be correctly administered.During the treatment process of inflammatory ocular surface diseases,the combination of NSAIDs with glucocorticoid drug can strengthen the curative effect and reduce the adverse reactions.

Objective To study the curative effect of major aphthous ulcer using povidone-iodine,H_2O_2 asso- ciated with levamisole liniment.Methods The RAU patients were divided into 2 groups randomly.The patients of curative group were instructed to use povidone-iodine,H_2O_2 and levamisole liniment;The control group were in- structed to take metronidazole,antibiotics,compound vitamin B and vitamin C.Then the patients were observed peri- odically.Results The success rate of curative group was 85 % and the control group was 55 %.The difference had statistical significance(P＜0.01).Conclusion Treatment of major aphthous ulcer using povidone-iodine,H_2O_2 and levamisole liniment is effective.

OBJECTIVETo analyze the changes in CIE L*, a*, and b* at cervical, body, and incisal sites after tooth bleaching by using a spectrophotometer.

METHODSSixty-seven intact and healthy maxillary central incisors were in-vestigated. These incisors were darker than A3 according to the Vita Classical shade guide. The CIE tooth shade parameters L*, a*, and b* were simultaneously recorded at three tooth areas (cervical, body, and incisal) with a spectrophotometer before and after tooth bleaching (35%H2O2 coordinating with Beyond whitening accelerator irradiating). The shade dif-ferential (DeltaE) was calculated. ANOVA, paired t-test, and Pearson correlation analysis were used for data analysis.

RESULTSThe efficacy rates of tooth bleaching were satisfactory, with 86.6%, 86.6%, and 85.1% in the cervical, body, and incisal sites, respectively. The average values of DeltaE were 5.09, 4.44, and 4.40 in the cervical, body, and incisal sites. Tooth bleaching significantly increased L* and significantly decreased a* and b* in all tooth areas (P < 0.01). The decreasing range of Deltab* was more than the increasing range of DeltaL* at the cervical site; opposite results were observed at the incisal site. A positive correlation was detected between baseline b* and DeltaE.

CONCLUSIONThe spectrophotometer could objectively evaluate the whitening effect of tooth bleaching at the different tooth sites. The tooth bleaching system (35%H202 coordinating with Beyond whitening accelerator irradiating) exerts powerful bleaching actions in most of the tooth areas investigated. The order of tooth bleaching effectiveness is cervicalbody>incisal. Yellow coloration is decreased mainly at the cervical site, and brightness was increased mostly at theincisal site. The effectiveness of tooth bleaching increases as the baseline b* value increases.

Objective To investigate the etiological factors and treatment of thoraco-gastric perforation (TGP) after esophagogastrostomy. Methods Retrospective analysis was carried out in 16 patients with thoraco-gastric perforation after esophagogastrostomy in our department from March 1974 to March 2004. The etiological factor, clinical feature, experiment test and the method of treatment were compared between TGP and patients of thoracic esophageal anastomosis leak (TEAL). Results Thoraco-gastric perforation occurs within 2～5 days postoperatively. Among these 16 cases, in 8, local necrosis of gastric wall was found which was caused by severe contusion and massive ligature. In 5, were penetrative injury of gastric wall caused by suture needle. In 3,unsuitable purse-string suturing of corner of greater or lisser gastric curvature. Hydropneumothorax occurred after thoraco-gastric perforation. Chest fluid was brown and turbid with putrefactive odor. With medium examination, anastomosis was normal, but medium and air bubbles were found outside of the stomach. Perforation were repaired and covered by pedicle tissue-flap in all cases. 15 cases were cured with no sequence. Conclusion TGP after esophagectomy were correlated with technique of surgery. TGP often occurs early after esophagogastrostomy. Transthoracic repairing of TGP should be done as soon as possible. The healing ratio of reparation was about 93.8%.

Objective To observe the mechanism of herbal cake separated moxibustion to hyperlipemia (HLP) and atherosclerosis (AS) rabbits on cell and molecular level. Method 75 rabbits were divided into 5 groups randomly:blank group, model group of HLP and AS, direct moxibustion group, herbal cake separated moxibustion group, herb group, 15 rabbits each group. Model rabbits of HLP and AS were built by immunity injury method. The expression of VCAM-1 mRNA and E-sel mRNA were determined with the method of RT-PCR. Results The expression of E-sel mRNA in herbal cake separated moxibustion group, direct moxibustion group and herb group are obviously decreased (P