Objective To investigate the effects of titanium nitride(TiN) coating on corrosion resistance of dental Co-Cr alloy in the simulated oral environment. Methods The Co-Cr alloy commonly used was casted with specimen sized of 10 mm× 10 mm×3 mm. Then the specimen was coated with a thickness of 2.5 μm TiN coating on the surface by multi-arc physical vapor deposition. The Tafel polarization curves of the specimen before and after coating titanium nitride in artificial saliva were measured by electrochemical station. Results From the Tafel polarization curves, the specimen after coating TiN showed that both anodic polarization curve and cathodal polarization curve were fiat, natural corrosion potential moved to the positive way, and passivation interval got longer. From the annular anodic polarization curves, the specimen after coating TiN had almost no "delay area". Conclusion TiN coating can improve corrosion resistance of Co-Cr alloy.

Public Health Ophthalmology is a population-,region-or country-based as a whole object,and is committed to improving the eye health of the whole population.Epidemiological investigation of the eye was named to the region from a single disease to variation of diseases from cross-sectional survey to a longitudinal cohort study.Epidemiological investigations is the most important way which can reflect the pathogenic factors for public health policy-making,early diagnosis and prevention and treatment of eye disease,and Research to Prevent Blindness.Imaging Diagnosis,quality control,improve the reliability of the epidemiological investigation,and to strengthen the study of disease,race,and molecular levels and create a unique epidemiological study.

Objective To explore economic valuation of the minimally invasive esophagectomy.Methods The cases undergone esophagectomy were studied retrospectively.Monofactorial analysis was done to evaluate the impact of various factors on the overall hospitalization costs.The correlated factors then were used as independent variables to carry out the multiple linear regression aimed at the hospitalization costs.Results The results of monofactorial analysis showed that the accompanying diseases,days during hospitalization,modes of esophagectomy,postoperative complications were correlated factors of the overall hospitalization costs.According to the multiple linear regression,it were sorted based on descending order duing to its effect of largenning the hospitalization costs:postoperative complications (β =0.439,P =0.000),days during hospitalization (β =0.397,P =0.000),accompanying diseases(β =0.257,P =0.000),modes of esophagectomy(β =0.132,P =0.000).The expenses caused immediately by the operations were statistically more of the minimally invasive surgery than that of the open procedures whatever the numerous or proportion of the overall costs (P =0.000).Conclusion Combined thoracoscopic and laparoscopic esophagectomy was more expensive than open operation.Nevertheless,impact of the other factors,such as postoperative complications,were more significant than that of the different procedure.h was a strategy to minimally invasive osophagectomy that hospitalization costs could be reduced by means of controlling complications and diminishing hospitalization days.

Objective To detect the differential expression profile of microRNAs between patients with or without gall?bladder stone. Methods Samples from 30 patients with gallbladder stones (GS) and 30 without gallbladder stones (GP) were collected, in which microRNAs expression profiles were examined using high-throughput sequencing instrument Illumi?na HiSeq 2500. MicroRNA sequences were obtained and compared to Genebank and Rfam database for classification. Differ?entially expressed microRNAs were screened, and their target genes were predicted. Significant enrichment analysis of GO and KEGG were performed. Real-time quantitative PCR was performed on selected miRNAs in order to validate their expres?sion. Results Clean tags were obtained from both GS group (n=2 215 832) and GP group (n=1 424 770). A total of 17 mi?croRNAs were differentially expressed between GS and GP groups with statistical significance, among which 9 were up-regu?lated and 8 were down-regulated in GS group compared to those in GP group. GO (Gene ocology) analysis showed that target genes were enriched in ion binding and transport, apolipoprotein binding, calcium channel activity, protein kinase activity, steroid hormone biosynthesis and metabolism. KEGG(Kyoto Encyclopedia of Genes and Genomes)analysis is shown for the target genes enriched in cancer related pathways, including WNT, HIPPO pathways. qRT-PCR validation of some differen?tially expressed miRNAs confirmed the result of high-throughput data analysis. Conclusion The differential expression levels of microRNAs may play an important role in occurrence and development of gallbladder stones.

Translational medicine, characterized by multi-disciplinary cooperation, is a new research area focusing on patients. Although translational medicine is still in its early stages in China, it has attracted increasing attention. To apply translational medicine, it is essential to implement the principles and methods of systematic biology to break the inherent barriers between basic and clinical science. Moreover, it is important to update research ideas, strengthen the transformation of scientific and technological achievements, and construct public library research resources and exchange platforms.

With the development of management in NICU,nosocomial infection increases gradually.Nosocomial infection monitoring is very important for improving the survival rate of the newborn and crutial for updating the management of NICU.This article discussed the risk factors and prevention strategies of nosocomial infection in NICU.

Objective To investigate the method and curative effect for the operative treatment of acetabular fractures.Methods Forty-one patients with acetabular fractures were treated operatively from March 2001 to March 2006.All fractures were classified according to the Letournel-Judet classification,22cases were simple fractures and 19 cases were complicated fractures.Kocher-Langenbeck (K-L) approach were adopted in 31 cases (14 cases through osteotomy of greater trochanter additionally),ilioinguinal approach were adopted in 8 cases and anterior combined posterior approach were adopted in 2 cases.Different fractures were fixed by reconstructive plates and screws.Results All the patients were followed up for 16-36 (24.31 ± 5.31 ) months.According to the reduction criteria of Matta radiography,there were 27 cases with anatomic reduction,8 cases with satisfactory reduction,3 cases with poor reduction and 3 cases with joint contour reduction.According to the Merle D' Aubigne clinical criteria for joint functions,14 cases showed excellent results,18 cases were good,5 cases were fair and 4 cases were poor,and the excellent-good rate was 78%( 32/41 ).Necrosis of the femoral head was found in 3 cases,heterotopic ossification in 4 cases,traumatic arthritis in 3 cases,sciatic nerve injury in 4 cases,femoral nerve injury in 1 case,all the nerve injury recovered in 6 months.Lower extremity deep venous thrombosis in 3 cases,all of them were recovered by given anticoagulation.No death,infection or nonunion were found.Conclusions K-L approach or ilioinguinal approach are adopted in the most of the operative treatment of acetabular fractures.Dual reconstructive plates or single.reconstructive plate combine lag screws can fix solid.It can make hip to do early exercise.

Objective In order to discuss the nursing points for chronic skin ulcer patients who had ac-cecpted local oxygen therapy and traditional chinese medicine treatment. Methods We gave totally nursing to 20 patients who accecpted local oxygen therapy and traditional chinese medicine treatment. Results Eighty percent patients had obtained clincal cure and got successful nursing effect. Conclusions Careful perioperation nursing and related monitoring can improve the cute rate of chronic skin ulcer, and then pre-vent complication.

Objective To investigate the efficacy of bilevel positive airway pressure mechanical ventilation (BiPAP) in the patients of acute organophosphorus pesticides poisoning (AOPP) with respiratory failure. Methods 44 AOPP patients with respiratort failure were randomly divided into experimental group (n = 22) and control group (n = 22). The routine trentment was given to both groups, additional BiPAP assisted ventilation was given to the treatment group. Blood gas,heart rate and respiratory rate were detected before and 2 h,24 h,48 h after treatment, and the rote of intubatinn,mortality,duration and cost of hospitalization were compared between two groups. Results Heart rate,PaO2 was significantly increased and PaCO2 decreased in BiPAP ventilation (P <0. 05). The mortality,intubatien rate and cost of hospitalization were significantly decreased in BiPAP treatment group (P < 0. 05). Conclusion The BiPAP ventilation is certainly effective to acute organophosphorus pesticides poisoning with respiratory failure. It could obviously improve the clinical symptoms and arterial blood gas analysis, and could reduce the mortaiity, rate of intubotion,cost of hospitalization of patients of acute organophosphoms pesticides poisoning with respiratory failure.

Cancer stem cells are a new field with the gradual deep understanding of the stem cell research. Although its history is not long, the rapid development showed a good potential prospect. The existence of cancer stem cells and its use as tumor formation, growth and metastasis-based approach has been widely recognized. In theory, cancer stem cells derived from normal stem cells can transform. Cancer-causing factors can also be derived-from the dedifferentiation induced cells. Because it contains all-round, multi-and single stem cell, with the proliferation and differentiation potential of different directions, it is possible to explain the heterogeneity of tumor cells, and reasons for hypoxic environment, anti-cancer drugs and radiation resilience. In this area, it has been made a lot of progress in recent years. However, there are still some problems to be solved. Such as the identification of cancer stem cells, biological function and mechanism.