Objective To investigate the diagnostic value of ThinPrep cytology(TCT) combined with colposcopy for cervical lesions.Methods 980 patients with suspected cervical lesions were selected and received TCT combined with colposcopy examination.Among the 980 patients,102 patients with positive smear were referred for colposcopy and were diagnosed by colposcopy biopsy.Results Of 102 patients with positive smear,the detection rates of the atypical squamous cells (ASC),low-grade squamous intraepithelial lesions (LSIL),cervical intraepithelial neoplasia (HSIL) and atypical glandular cells (AGC) were 6.1％,0.6％,2.1％ and 1.5％,respectively.Compared with the colposcopy biopsy for cytology samples,the test results were as follows:LSIL was 72.4％ (21/29),HSIL was 93.8％ (15/16).Conclusion The method of TCT could increase the screening rate of positive diagnosis of cervical lesions and for the highly suspected patients,the combination of vaginal colposcopy and biopsy could further improve the accuracy of diagnosis,in order to provide scientific solutions for clinical treatment.

ObjectiveTo evaluate effects of post-operative analgesia combined with parental presence on emergence agitation after sevoflurane anesthesia in children. Methods60 patients were randomly assigned into three groups with 20 cases each. After sevoflurane inhalation and being given rocuronium, all patients were intubated for mechanical ventilation. Then patients received caudal block. Patiens in group A and B were treated with diclofenac sodium suppositories before operation. To maintain anesthesia patients were adopted sevoflurane inhalation. Patients in group A and C were cared by nurses in post anesthesia care unit, whereas patients in group B added parental presence. Emergence agitation incidence, modified Aldrete scale, PAEDS, CHEOPS were all recorded. ResultsTherewas no difference of modified Aldrete scale among three groups( F =0.652 ,P ＞0. 05) ,but emergence agitation incidence in group C was more than those of group A and B. At each point PAEDS scores of group B were lower than those of group C( x2 =4. 566,7. 234,4. 124,6. 126, P ＜ 0. 05) ,also scores of group B were lower than those of group A at 10 min and 30 min(F=0.654,P＞0.05). PAEDS scores of group A were lower than those of group C at 20min and 30min(t =3.256,3.778,4.556,4.223 ,all P＜0.05). At 20min and 30min CHEOPS scores of group C were higher than those of group A and B ( F =1. 987, P ＞ 0.05 ). ConclusionPost-operative analgesia combined with parental presence could decrease the incidence of emergence agitation after sevoflurane anesthesia in children.

High mobility group protein A (HMGA) plays a critical role in the development of a variety of tumors.It's expression level will abnormally increase with the increase of the malignant degrees of astrocytoma and metastasis potential.Let-7 which is a kind of small RNA endogenous coding negatively regulates HMGA genes as a tumor suppressor.The expression of let-7 reduces while HMGA rises in many kinds of tumors was obversed.Therefore,this finding provides new references for the early diagnosis of tumor and also provides new approaches for therapy of tumor.

Lung cancer is the leading cause of morbidity and mortality of malignant tumor.Surgery,radiotherapy and chemotherapy remain the primary means of treatment for lung cancer,while the discoveries of molecular targets and targeted agents for lung cancer are making revolutionary changes and will almost certainly propel progress in this field into the foreseeable future.Biomarker-based individualized comprehensive treatment strategy is expected to benefit the lung cancer patients' survival.Meanwhile,chemotherapy remains the major treatment of advanced non-small cell lung cancer,which requires implementing the "three combinations" dialectical therapy principles,that is,systemic with local,conventional with personalized,eliminating with supporting.

Objective To evaluate the effect of ketamine and elonidine on P2X4R mRNA expression in the spinal cord in a rat model of neuropathic pain.Methods Eighty male SD rats weighing 180-220 g were randomly divided inte 5 groups (n = 16 each): sham operation group (group S),neuropathic pain group (group NP),ketamine group (group K),clonidine group (group CL) and ketamine + clonidine group (group KC).The animals were anesthetized with intraperitoneal chloral hydrate 400 mg/kg.The right sciatic nerve was exposed and four ligatures were placed on the sciatic nerve at 1 mm interval.In group S,the right sciatic nerve was exposed but not ligated,and in the other groups four ligatures were placed around the right sciatic nerve (chronic constrictive injury,CCI) as described by Bennett.The animals were injected intraperitoneally with ketamine 10 mg/kg,clonidine 1 mg/kg,and ketamine 5 mg/kg + clonidine 0.5 mg/kg in normal saline 2 ml in group K,CL and KC respectively,while normal saline 2 ml in group S and NP at 3-21 d after CCI.Mechanical and thermal pain threshold were measured by paw withdrawal latencies to run Frey hair and radiant heat stimulation at 1 d before and 3,7,14,21 d after CCI.Four animals were killed at 1 d before (baseline) and 7,14 and 21 d after CCI following the measurement of pain threshold in each group and the L4.5 segments of the spinal cord were removed for determination of P2X4 R mRNA expression by RT-PCR.Results There was no significant change in thermal and mechanical paln threshold and P2X4 R mRNA expression in the spinal cord after CCI in group S ( P > 0.05),while thermal and mechanical pain threshold was reduced,and P2X4 R mRNA expression was up-regulated in the other four groups as compared with the baseline values (P < 0.05 ).Thermal and mechanical pain threshold was significantly higher and P2X4 R mRNA expression in the spinal cord lower after CCI in group K,CL and KC than in group NP ( P < 0.05 ).Thermal and mechanical pain threshold was significantly higher and P2X4 R mRNA expression lower after CCI in group KC than in group K ( P < 0.05),but no significant difference was found in the above parameters between group CL and K ( P > 0.05).Conclusion Both ketamine and clonidine can alleviate neuropathic pain via down-regulating the expression of P2X4 R mRNA in the spinal cord in a rat model of neuropathic pain.

Drug addiction is a chronic recrudescent brain dis-ease. Various addictive drugs acting on the reward system result in rewarding effects through changes in neurotransmitter patholog-ical release. Among these monoamine neurotransmitters, 5-hydroxytryptamine, norepinephrine and dopamine play key roles in drug addiction. This paper reviews, from a comprehensive perspective, the roles which monoamine neurotransmitters play in the drug addiction and the process of getting addictive.

[Summary] Pectus excavatum ( PE ) is a posterior intrusion of the chest wall into the thoracic cavity . Although the physiological and psychological consequences vary , for a large number of patients the lesions are troublesome enough that they need corrective surgery .The minimally invasive repair of pectus excavatum ( Nuss procedure ) has been performed safely , effectively , and extensively .The current situations and progresses associated with the Nuss procedure were reviewed .

Aiming to improve the quality of teaching international students and enhance the international cooperation of medical teaching,this article discusses the teaching to international students from the aspects such as enhancing the recognition,breaking through language communication obstacles,careful preparation and teaching,and strict regular management by combining practice and research obtained from recent years of international student teaching.

Objective: To study the effect of spironolactone, an aldosterone receptor antagonist (ARA), on neuroendocrine of SD rats with dilated cardiomyopathy, so as to investigate its mechanism in treatment of dilated cardiomyopathy. Methods: Male SD rats, weighting (300±50) g, were intraperitoneally injected with adriamycin (2 mg/kg) once a week for 8 weeks to induce dilated cardiomyopathy models. The rat models were randomly divided into 4 groups: control group, low dose spironolactone group (10 mg/[kg·d], i. g.], middle dose spironolactone group (20 mg/[kg·d], i. g.) and high dose spironolactone group (40 mg/[kg·d], i. g.). Rats in the normal control group received normal saline (10 mg/[kg·d], i. g.). Spironolactone was given for 8 weeks. Angiotensin II(Ang II) and aldosterone (ALD) levels in the plasma and cardiac muscle homogenate were determined 9 weeks later. The plasma nitrogen monoside (NO), N-terminal popeptide of collagen type III (P III NP) in serum and atrial natriuretic peptide (ANP) in the plasma were also measured. Results: Eight weeks after treatment, the Ang II levels in the plasma of animals in the middle dose group and high dose group were significantly decreased(P<0.05), the levels of ALD were elevated(P<0.05), those of P III NP and ANP were decreased(all P<0.05). Conclusion: Spironolactone at 20 mg/(kg·d) or 40 mg/(kg·d) for 8 weeks can correct the disorder in neuroendocrine system to some extents.