Purpose: Repopulation is among those which causes treatment failure in cancer radiotherapy. The study focuses on a better understanding of radiation-induced repopulation for cancer cells.Materials and Methods: It was measured by BrdUrd/DNA flow cytometric analysis that the kinetic changes after various radiation dose-time effects on human nasophayngeal carcinoma cells. The surviving fraction of the clonogenic cells was also measured by limiting dilution method.Results: (1) the shortening of Tpot of overall cell population after 0～8 Gy of irradiation was closely related to the decrease of the clonogenic cells;(2) an increased DNA synthesis was suggested as a result of the increase of radiation dose and (3) in vitro ,the proliferative status of the clonogenic cells was as same as that of overall cell population 48 hours after irradiation.Conclusion: (1) the accelerated repopulation of CNE cells after irradiation is related to their self-regulation;and (2) Tpot may be used as a predictive value in clinical radiotherapy.

Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model of hippocampal slice in neonatal rats in vitro,Methods:Hippocampal slices(400?m)from 8~10-day-old rats are transferred into an incubator with insert of Millicell membrance and incubated for 7 d,14 d,20 d and 30 d,respectively.The growing state of the hippocampal slices were observed under the convened microscope,and its ability to absorb propidiumiodide (PI) was detected under fluorescent microscope after the hippocampal slice was stained by PI,and the state of the hippocampal slices at different time after aerating Nitrogen gas was observed. Results: ① No PI labelled cells were found after 7 d and 14 d. However,15% PI positive cells were found after 30 d. ②The longer in Nitrogen gas,the more apoptosis cells. ③Intensive fluorescence was observed in the CA1,CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h. Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampl slice in vitro was successfully established after aerating Nitrogen gas for 1.5 h.

Objective:To introduce an economical and pragmatic method of establishing oxygen-glucose deprivation model for hippocampal slice of neonatal rats in vitro. Methods: Hippocampal slices(400?m) from 8~10-day-old rats were transferred into an incubator with insert of Millicell membrance and incubated for 7d,14d,20d and 30d respectively.Then they were observed under convened microscope to evaluate their growing state,and detected their ability to absorb propidiumiodide(PI) under fluorescent microscope after they had been stained by PI , The state of the hippocampal slices was observed at different time after aerating Nitrogen gas. Results:(1)No PI labelled cells were found after 7d and 14d.However,15% PI positive cells were found after 30d.(2)The longer in Nitrogen gas ,the more apoptosis cells.(3)Intensive fluorescence was observed in the CA1, CA3 and DG areas of the hippocampal slice after aerating Nitrogen gas for 1.5h.Conclusion:The hippocampal slice can be alive for about 30 days.Stable oxygen-glucose deprivation model of the hippocampal slice in vitro was successfully established after aerating Nitrogen gas for 1.5h.

AIM:To observe the effects of edaravone on high glucose-induced apoptosis of SH-SY5Y cells and its potential mechanism .METHODS:The SH-SY5Y cells were cultured in the DMEM medium with 100 mmol/L glucose and 100μmol/L edaravone for 24 h.The viability of the SH-SY5Y cells was detected by MTT assay .The levels of ROS in the cells were determined by DCFH-DA fluorescent probing .The apoptotic rates of the cells were analyzed by flow cytome-try.The protein expression of Bax and Bcl-2 in the cells were detected by Western blot .The expression levels of micro-RNA-25 (miR-25) were determined by real-time PCR.To further clarify the target sites of edaravone on inhibiting apopto-sis induced by high glucose , miR-25 inhibitor was applied to the SH-SY5Y cells and the activity of caspase-3 was meas-ured.RESULTS:Compared with control group , the cell viability was decreased significantly in model group , and the ROS level was increased significantly .The protein expression of Bax was up-regulated significantly , while the expression levels of Bcl-2 and miR-25 were significantly down-regulated .Compared with model group , the cell viability was increased signifi-cantly in edaravone group .The ROS level was decreased significantly .Meanwhile, the expression of Bax was down-regula-ted, while the expression of Bcl-2 and miR-25 was up-regulated with statistical significance .The caspase-3 activity of the cells incubated with 100 mmol/L glucose and miR-25 inhibitor was increased .However, no alteration of caspase-3 activity with edaravone added simultaneously was observed .CONCLUSION: Edaravone inhibits the apoptosis of SH-SY5Y cells induced by high glucose with the potential target site of miR-25.

This study aimed to explore clinical features of multiple myeloma in patients over 80 years old. Methods: We retrospectively analyzed 11 cases of multiple myeloma over 80 years old, which were diagnosed from 2000 to 2013 in our hospital. Results:The mean age was 83.5 years. All patients had more than two complicated diseases, and the performance status was poor in the majority of the patients. Ten cases received individualized treatments. The results showed that three patients had progressed, one patient achieved complete remission, three patients achieved partial remission, and four patients achieved minor remission. Median survival time was 28 months. The causes of death included six progressed myelomas, three pneumonias, and two acute myocardial infarctions. Conclusion: Patients aged 80 years old with myeloma are often at a late stage. Treatment should be individualized based on patient status. Survival time was evidently prolonged in very elderly patients with MM after individualized treatments, particularly in patients in stagesⅠandⅡ.

Lysyal oxidase (LOX),a cooper dependent monoamine oxidase,can stabilize the extracellular matrix and is closely related to the tumor cell differentiation,vicious transformation and invasiveness.LOX is secreted into extracellular,and it is resolved into a small fragment by sol protease,which is lysyal oxidase propeptide (LOX-PP).According to research,LOX-PP has its own structure and physiological functions,which can inhibit the growth of tumor cells.LOX-PP also plays a significant role in the generation and progression of tumor,which is likely to be a new therapeutic target.

CD72 is a B cell specific receptor that exists in multiple alternative splicing forms. Eight novel alternative splicing forms of CD72 were identified from the spleenocytes of BALB/C mice. Two very unique intron sequences were found in those alternative splicing forms. One kind of splicing variants retained the intron1 in the mRNA. This intron can be translated into 32 amino acid residues without changing the reading frame of the whole proteins. Another kind of splicing variants used an alternative 3' splice site in intron 3(3'AS) which led to premature termination of its encoded protein. The differential expression of the CD72 splicing variants were compared in BALB/C and NZB/W mice that were at different stage of systematic lupus erythematosis(SLE) disease development. It was found that 1) splicing forms containing 3'AS was rare in all samples examinated; 2) splicing forms containing two ITIM domains and transmembrane domains were more abundant in BALB/C mice than in NZB/W mice, even in some cases the two ITIM domains were separated by the intron 1; 3) a shorter splicing form with both exon2 and exon3 missing was expressed highly in terminally diseased NZB/W mice.These results suggested an important role of CD72 alternative splicing forms in B cell receptor signaling and in SLE.

Objective To evaluate the clinical effectiveness and pseudoaccommodation in the early stage after AcrySof ReSTOR multifocal intraocular lens (IOL) and monofocal IOL implantation.Methods A total of 138 patients (158 eyes) with age-related cataract underwent phacoemulsification and IOL implantation were chosen,73eyes of 60 patients with a multifocal IOL implantation as observation group and 85 eyes of 78 patients with monofocal IOL implantation as control group.The uncorrected visual acuity,best corrected visual acuity was evaluated at 1 week,1 month,and 3 months after operation,pseudoaccommodation and quality of life was evaluated at 3 months after operation.Results The patients of both observation group and control group were able to obtain a satisfactory distance vision at 1 week,1 month,and 3 months.The uncorrected intermediate visual acuity of observation group were 0.49 ± 0.19,0.72 ± 0.21,0.77 ±0.23,which of control group were 0.24 ± 0.21,0.27 ± 0.22,0.28 ± 0.24,there were statistical differences between two groups (all P ＜ 0.05).The uncorrected near visual acuity of observation group were 0.47 ± 0.20,0.70 ± 0.22,0.80 ± 0.24,which of control group were 0.21 ±0.23,0.23 ±0.19,0.26 ±0.18,there were statistical differences between two groups (all P ＜ 0.05).There was no significant difference in the uncorrected near visual acuity,best corrected distance,intermediate and near visual acuity between observation group and control group (all P ＞ 0.05).At 3 months,the distance and near pseudoaccommodation of observation group were (2.56 ± 0.82) D,(3.19 ±1.13)D,which of control group were (0.87 ±0.57)D,(1.03 ±0.59) D,there were statistical differences between two groups (all P ＜ 0.05).At 3 months,VF-14 scale score and wearing glasses as near score of observation group were 92.21 ±4.22,4.23 ±0.85,which of control group were 71.23 ± 3.96,2.01 ± 0.71,there were statistical differences between two groups (all P ＜ 0.05).Conclusion AcrySof ReSTOR multifocal intraocular lens implantation can provide a higher level of visual acuity,pseudoaccommodation and satisfaction compared with monofocal intraocular lens.

Aiming at the technical characteristics and transforming difficulties for patents in medical health industry,this paper makes proposition on establishing a service system for patent transformation in healthcare system and accelerating the transformation and clinical application of medical technologies through the enhancement of technical service platform construction,strengthening of professional staff capabilities,and the improvement of patent values assessment.

Objective: To observe the effect of puncturing Juliao (GB 29) in treating carpal joint sprain. Methods: 99 cases were randomly divided into 3 groups, treatment, control 1 and control 2 groups, 33 cases for each group. Cases in treatment group were treated by acupuncture on Juliao (GB 29); patients in control 1 group were treated by acupuncture at Sanyinjiao (SP 6), and patients in control 2 group were treated by local application. The clinical effect was compared among three groups after the treatment. Results: The effective rate in the treatment group is 96.9%, which is over and above 84.8% in control 1 group and 75.8% in control 2 group (both P＜0.05). Conclusion: Acupuncture at Juliao (GB 29) has a precise curative effect in treating sprain of carpal joint. It is more effective than acupuncture at Sanyinjiao (SP 6) and local application.