Objective To present a new seriological method for diagnosing breast carcinoma.Methods Seral anti-p53 antibody was detected with immuno-polymerase chain reation(Immuno-PCR).The expression of p53 in tissue was detected with enzymeimmu-nohistochemistry technique.Results The positive rate of anti-p53 antibody in serum of breast carcinoma was 39.5% and anti-p53 antibody was not found in non-carcinoma and normal controls.The positive rate of anti-p53 antibody was higher than that of non-carcinoma and normal controls(P

Objective:To investigate the mechanism of ursolic acid inducing the apoptosis of human nasopharyngeal carcinoma cell line CNE-2Z.Methods:CNE-2Z were treated by ursolic acid with different concentration.Cell proliferation was determined by MTT assay.Cell cycle and apoptosis rate were analysed by flow cytometry(FCM).The expressions of bax,bcl-2 and cox-2 were assessed by SP method of immunocytochemistry,while the expression of caspase 3 by Western blot.Results:Ursolic acid inhibited the proliferation of CNE-2Z cells and induced the apoptosis in dosage and time dependent manner.FCM showed that the cell population reduced in S phase and cell cycle was arrested in G0/G1 phase.Immunocytochemistry showed that the expression of bax was up-regulated in the apoptosis,while the expression of bcl-2 and cox-2 down-regulated.Western blot showed that the expression of caspase 3 was strengthened.Conclusion:Ursolic acid can induce apoptosis of CNE-2Z in vitro.Its molecular mechanism probably is through promoting activation of caspase3,up-regulating the expression of bax,and down-regulating the expressions of bcl-2 and cox-2.

Objective To compare the content of saponin in Bark of Silktree Albizziae and Bark of Silktree Albizziae kaldora derived from different collection periods.Method Spectrophotometry was used to determine the contents of saponin.Result The contents of saponin in one-year Bark of Silktree Albizziae is different from two-year Bark of Silktree Albizziae(P0.05).The contents of saponin in multi-year Bark of Silktree Albizziae is different from multi-year Bark of Silktree Albizziae kaldora(P

Objective:To identify differential proteins expression of seminal plasma of varicocele patients and normal person,providing evidence for further exploring the molecular mechanism of infertility caused by varicocele.Methods:The differential proteins expression of seminal plasma of varicocele patients and normal person were characterized by means of two-dimensional gel electrophoresis(2-DE).Results:The well-resolved,reproducible 2-DE seminal plasma were obtained.The 38 differential protein spots were identified.Conclusion:The male infertility might due to the expression and modification of some proteins in seminal plasma of varicocele patient.Further analysis on the different proteins'expression helps revealing molecule mechanism of varicocele causing male infertility.

Objective:To study the influence of rt-PA and urokinase on the expression of matrix metalloproteinase(MMP-9) and hemorrhagic transformation after thrombolysis therapy in rats.Methods:A focal cerebral ischemia model of rat was established through embolization of left middle cerebral artery with autologous blood clots.rt-PA and urokinase were intravenously administrated 6 hours after ischemia at a dose of 10 mg/kg and 4 000 ?/kg.At the 24th hour after ischemia,all rats were killed.The expression of MMP-9 was detected with immunohistochemical staining and cerebral hemorrhage was quantified by spectrophotometer assay.Results:The expression of MMP-9 was increased after ischemia,and MMP-9 was significantly further upregulated after rt-PA and urokinase had been intravenously administrated.The average optical density(AOD) of MMP-9 in the sham operated group was 0.086?0.008,and increased to 0.158?0.010 after cerebral ischemia for 24 hours and then further increased to 0.225?0.034 and 0.228?0.022 in the rt-PA and urokinase thrombolytic therapy groups.Transformation and quantified intracerebral hemorrhage were significantly higher in the therapy groups as compared with the ischemic group.However,there was no difference between rt-PA group and urokinase group.Conclusion:rt-PA and urokinase treatment both increase the levels of MMP-9 after ischemia.MMP-9 might be involved in the hemorrhagic transformation after thrombolytic therapy with rt-PA and urokinase.

Objective:Evaluate the relationship of complications and indwelling time of double-J ureteral stents after ureterscopic holmium-laser lithotripsy. Methods:170 patients were divided into two groups randomly. groupⅠ(stents indwelling 4 weeks),groupⅡ(stents indwelling 2 weeks).Complications of two groups were compared. Results:During the indwelling time,two groups presented urinary irritative sypmtoms were 81(93%)and 51(61%)P

Hematopoietic stem cell transplantation (HSCT) has become the frontline therapy for patients with multiple myeloma (MM), and for young patients with primary MM, early autologous stem cell transplantation (ASCT) is a standard treatment. In the era of novel drugs, the application of new pharmaceutics in the consolidation and maintenance therapy before and after HSCT can improve the efficacy of HSCT. The novel agents pose a challenge to the status of HSCT in relapsed and refractory myeloma. This article summarizes the recent clinical studies of HSCT in the era of novel agents to clarify the role of HSCT in treatment of MM.

AIM: To observe the adverse effect and clinical efficiency of Y-shape plate internal fixation for treating comminuted distal humeral fracture. METHODS: A retrospective analysis was conducted in 18 patients with the comminuted distal humeral fracture,who were admitted in the First Affiliated Hospital of Nanyang Medical College from January 2003 to December 2006. All patients were treated with Y-shape stainless steel or Ti-alloy plate in 3-7 holes,then the response of materials and host was observed. The curative efficiency was assayed with Cassebaum score,and the healing of fractures was detected with X-ray film. During follow-ups,the pain and joint motion were observed. RESULTS: The mean operational time was 134 minutes,the mean value of intraoperative blood loss was 172 mL,and all the incisions were healed well. All the patients were followed up for 6 months,and the mean time for the fracture healing was 14 weeks. One case appeared delayed healing in the resection lesion,and Kirschner's wire was loosened in 2 cases. There was no infection,local inflammatory reaction or rejection reaction occurred. Among 18 patients,11 got excellent results,4 good,2 fair,1 poor,and the rate of excellent and good efficiency was 83.3%. CONCLUSION: Y-shape plate after implantation shows good biocompatibility with the human body,with the manifestations of good reduction of comminuted distal humeral fracture,firm internal fixation,and satisfactory functional recovery of elbow joint.

Objective To discuss the change and the regulation of tumornecrosis factor(TNF-?) and epidermal growth factor(EGF) before and after extracorporeal shock wave lithotripsy.Method Radioimmunoassay was used to measure TNF-? in 50 cases urine sample before and after ESWL.Results TNF-? level in urine after ESWL were increased significantly at 1～4d(t=2.82,P

Objective To compare the content of total flavonoids in Herba Scutellarlae Barbatae derived from different places.Method UV metric method was used to determine the contents of total flavonoids of Herba Scutellarlae Barbatae from different regions.Result The contents of total flavonoids in Herba Scutellariae Barbatae derived from Nantong Jiangsu,Juaoling Guangdong,Lixin Anhui,Huaning Hunan, Mojiang Yunnan,Yongding Fujian,Nanchang Jiangxi,Longsheng Guangxi,Honghu Hubei and Qingyuan Zhejiang were 4.16%,3.55%,2.89%,2.78%,2.41%,2.22%,2.16%,1.98%,1.61% and 1.51%,respectively. Conclusion The content of total flavonoids in Herba Scutellariae Barbatae from different places is quite different,and the highest is the one from Nantong Jiangsu.