Objective Using siRNA to downregulate Her2 expression in Her2 positive breast cancer cell line SK-BR-3,to observe the influence of siRNA of cell proliferation and apoptosis. Methods Two siRNAs against the Her2 gene were designed and transfected into SK-BR-3 cells respectively, Her2 gene expression was measured using RT-PCR, Her2 protein expression was analysed using flow cytometer. Cell proliferation was measured with MTT assay, the apoptotic cells were stained with annexin-V-FITC and analysed by flow cytometry. Results 42.88%,49.27% silencing of Her2 mRNA expression were observed after transfecting with two siRNA respectively. The corresponding decrease of Her2 protein expression was confirmed by flow cytometric analysis. As a consequence, cell growth inhibition was observed,28.51% and 42.81% of cells apoptosis were observed after transfecting with two siRNA respectively.Conclusion These results demonstrated that siRNA against Her2 could effectively downregulate Her2 expression in SK-BR-3 cell line, inhibit cell growth and induce cell apoptosis.

Objective To investigate the effect of steroid hormones on lymphatic stomata of ovarian bursa and fluid adsorption. Methods The lymphatic adsorption was traced by trypan blue,and the ultrastructure of the lymphatic stomata and estrogen receptor level of mesothelial cells on ovarian bursa was observed by scanning electron microscopy,transmission electron microscopy and immuno-colloid golden technology. Results The absorption and opening area of lymphatic stomata were enhanced in estrogen group mice,and decreased in androgen group as compared to control group mice.Estrogen receptor was first identified in the nuclei of mesothelial cells on the inner layer of mice ovarian bursa and the level was down-regulated by estrogen and slightly up-regulated by androgen.Conclusion The absorption and the opening area of lymphatic stomata can be regulated by steroid hormones in mice,and the machanism may be related to the expression levels of estrogen receptor in the nuclei of mesothelial cells.

In fifteen human fetuses spcimens the peritoneal stomata were studied with SEM and TEM, and measured by image processing system. In order to prove that the peritoneal stomata are the passageway of absorbed matter from the peritoneal cavity, animal experiments were made. There are two types of the mesothelial cells on diaphragmatic peritoneum, i. e. the cuboidal cells and the flattened cells. The peritoneal stomata, which arranged in clusters or strips, were only found between the cuboidal cells. The shape and size of the stomata were often irregular. The average area of the stoma on the muscular portion is 10.43?1.61?m~2, while on the tendinous portion is 7.93?1.67?m~2. The connective tissue underlies below the stomata, under which no basement membrane was found. Many lymphatic capillaries were observed in the connective tissue, which may promote absorption of matter from the peritoneal cavity. In animal experiments, some particles of trypan blue were absorbed through the stomata of rabbit diaphragmatic peritoneum. The authors consider that the stomata, are first observed in human, are important pathway for draining matter from the peritoneal cavity.

By transmission electron microscopy and freeze etching technique 15 human fetuses were utilized to study the ultrastructure of the mesothelial cells on the parietal peritoneum. The mesothelial cells of the diaphragmatic peritoneum contained numerous vesicles which were frequently communicated with the free surface, the basement membrane, intercellular space and the peritoneal stomata. Some of the vesicles seemed to fuse each other and form vacuoles. Vacuoles also occurred close to, or communicated with the basement membrane and cell free surface. Sometimes they appeared as secretory particles. The microvilli contained vesicles opened to the free surface. The mesothelial cells on the pelvic wall displayed abundant endoplasmic reticulum and Golgi apparatus, but scanty vesicles. So, the mesothelial cells on parietal peritoneum of human fetuses might be classified into two types, i. e. the vesiclecontaining cells on the diaphragmatic peritoneum and the ER-containing cells on the peritoneum of the pelvic wall. The vesicle-containing cells seemed to uptake material from the peritoneal cavity. Abundant endoplasmic reticulum and Golgi apparatus reflected a high synthetic activity, hence the ER-containing cells might be possibly related to the production of peritoneal fluid.

Objective To study the ultrastructure of ovary lymphatic stomata in human and compared with the animals′. Methods The morphology of human ovary surface was examined using scanning electron microscopy and the lymphatic stomatas were counted by the ELESCOPE computer image processing system. Results The human ovary epithelium was composed of cuboidal and flat cells. There were lymphatic stomatas not only among the cuboidal cells, but also among the cuboidal and flat cells. The stomata were usually circular in shape and about 1 80?0 82??m in diameter, 6 27?2 65??m in circle. Lymphatic stomata in external surface of ovary mucinous tumor was also found. Conclusion There are ovary lymphatic stomatas in human. These stomatas connect lymphatic vessels in ovary with the peritoneal cavity. They also present morphological basis for early\|metastasis of ovary tumor. [

0.864, P

Objective To investigate the application of delayed sternal closure in the process of open heart surgery.Methods Delayed sternal closure was performed from Mar.2000 to Jun.2005 in 11 patients(4 males and 7 females,aged 16-67 years with an average of 42 years) after cardiac surgery.The indication included: cardiac dilatation(4 cases),intractable arrhythmia(4 cases),continuous bleeding(2 cases) and severe pulmonary edema(1 case).During the open chest period,the wound was covered with 3-layers of latex temporarily,and the delayed sternal closure was performed when bleeding was controlled,heart size reduced,and hemodynamic condition became stable.Results Except two patients died of acute kidney failure during open chest period,delayed sternal closure was successfully carried out in 9 patients 8-43 hours(23.7?11.0) after cardiac surgery.Except one patient died of cardiac arrest 21 days after operation,the 8 others survived and were discharged from ICU 1-8 days(3.9?2.4) after sternal closure,and the wounds healed well.Follow-up for the 8 survivors revealed an improvement of NYHA class(class Ⅰfor 4 cases,class Ⅱfor 2 cases,and class Ⅲ for 2 cases).Conclusion The delayed sternal closure is an effective method in the treatment of postoperative cardiac compression,severe bleeding and arrhythmia,and it does not increase the incidence of complications such as sternal infection.

Objective　To study the possibility of measuring health status for residents in rural areas by the Chinese SF-36 scale. Methods　Health status for 207 residents in rural areas of Fuyang city who were 14 years old and over was measured by the Chinese SF-36 scale. The reliability and validity were analyzed. Results　The internal consistency reliability coefficients (Cronbach'α) exceeded 0.50 for all scales except SF and RE scales. Inter-scale correlation coefficients were lower than Cronbach'α coefficients for all scales except SF scale. Factor analysis identified three principal components, which could be used to explain 64.6% of the total variance. The differences of scale scores between the young group and the middle age group weren't significant. There weren't significant differences of scale scores between sexes except RP scale. The differences of scale scores between smokers and no-smokers, drinkers and no-drinkers weren't significant. Nor were the differences between various educational levels. Conclusion　Whether the Chinese SF-36 scale is suitable for measurement of health status for residents in rural areas needs further studies to give more evidences.

Objective To investigate the relationship between expression of REG Iα gene and its clinicopathologic parameters, the survival rate in the patients with primary gastric cancer. Methods Using RT-PCR after extracting RNA from paraffin-embedded materials and immunohistochemical techniques, REG Iα gene expression was investigated in 235 samples. And the relation among their results with the clinicopathologic parameters of primary gastric carcinoma was discussed in experiment by SPSS 13.0 statistic software. Results The positive REG Iα mRNA was 78% (183/235) of primary gastric carcinoma and the positive rate of REG Iα protein was 31.1% (73/235) in 235 patients with primary gastric carcinoma. REG Iα gene expression in infiltrating tumors was found to be significantly higher compared with localized tumors ( P <0.05). REG Iα gene was closely linked to the infiltrative growth pattern, signet ring cell and poorly differentitated adencarcinoma. The incidence of venous invasion of REG Iα gene-positive differentiated adenocarcinoma was significantly higher than that of REG Iα gene-negative tumors. Moreover, the patients with REG Iα gene-positive differentiated adenocarcinoma were found to have a significantly poorer prognosis as compared with REG Iα gene-negative tumor patients. Conclusions The results of the experiment demonstrated that the expression of the REG Iα gene is closely related to the infiltrating property of primary gastric carcinoma. Signet ring cells and poorly differentiated adencarcinoma significantly enhance the risk of venous invasion of REG Iα gene-positive differentiated adenocarcinoma and might be as a prognostic indicator of differentiated adenocarcinoma of the stomach in clinic diagnosis.

From the leaves of Rabdosia inflexus (Thumb.)Hara.,a new diterpenoid,named inflexusin B,together with a known compound,oleanolic acid was isolated. Its structure was established by spectroscopic and chemi　cal evidence as Ⅰ. Inflexusin B showed significant cytotoxicity against Ehrlich carcinoma cell in vitro