Objective To investigate the association between a few single nucleotide polymorphisms(SNPs) in Fc receptor like 3 and ankylosing spondylitis(AS) in Hah nationality of China patients.Methods One hundred and sixty AS patients along with one hundred and seventy healthy controls were included in the study. SNPs of FcRL3-1 (rs0158440), FcRL3-2 ( rs2225828 ), FcRL3-3 ( rs7528684 ),FcRL3-4(rs11284799), FcRL3-5(rs945635), FcRL3-6(rs3761959), FcRL3-7 (rs2210913), FcRL3-8 (ra2282284) and FcRL3-9 (rs2282283) in FcRL3 gene were genotyped by MALDI-TOF technology. Haplotypes were estimated using PHASE v2.1 software. Results The frequency of FcRL3-1-1651C, FcRL3-3-169C, FcRL3-6 Intron3A and FcRL3-7 Intron3A alleles in AS patients were significantly increased and the frequency of FcRL3-2-641C, FcR-L3-4-110A and FcRL3-9 Intron15A were significantly increased compared with healthy controls. The frequency of FcRL3-1→9 CTCGCAAAA, CCCGGGGAA, CCCGCAAAC and CTCGCAAAC haplotypes were significantly increased and the frequency of FcRL3-1→9CCTGGGAA and TCCACAAGA haplotypes were significantly decreased compared with healthy controls. Conclusion These results suggest that FcRL3 may be associated with AS in Han nationality of China.

Objective To explore the role of Th17/IL-17 in the pathogenesis of asthma in children.Methods Thirty asthma children (asthma group) and 20 healthy volunteers (control group) were selected as our subjects.Percentage of Th17 cell in peripheral blood mononuclear cell (PBMC) of asthma children was detected by flow cytometry,the level of IL-17 and IgE in plasma were examined by enzyme linked immunosorbent assay.Results Proportion of Th17 cells in PBMC of children in asthma group was (1.83 ± 1.01) ％,significantly higher than that in control group ((1.02 ± 0.49) ％ ; t =3.896 ; P ＜ 0.01).The IL-17 level in control group was (19.11 ± 3.23) ng/L,significant lower than that in asthma group ((34.23 ± 4.88) ng/L;t =6.261 ;P ＜ 0.01)).The Ig E level in asthma group was (399.4 ± 45.1) ng/L,significantly higher than in control group((58.2 ± 19.7) ng/L;t =7.244,P ＜0.01).The level of IL-17 in plasma had a postive relationship with the proportion of Th17 cells(r =0.882,P ＜0.01),but not relationship was seen with the level of IgE (r =0.375,P ＜ 0.05).Conclusion Th1 7 cell is associated with asthma development of children,and the reason may due to probably aggravating asthma condition through increasing inflammation secreting IL-17.

Objective To observe the effect of ACh on outwardly rectifying potassium currents in isolated outer hair cells(OHCs) with variant length and to analyze effects of ACII on kinetics of the currents. Methods The whole cell patch-clamp technique was used. Results 100 ?mol/L ACh induced greater effects on outwardly rectifying potassium currents in short OHCs. The maximal amplitude of the outward currents increased by 34.8% in the presence of 100 ?mol/L ACII when conditioning potential was 50 mV. ACh effects on the peak currents were greater when compared to the effects on the steady-state currents and changed the shapes of the currents. ACII caused a hyperpolarizing shift of zero current potential of 5 mV. Kinetics of the currents were changed by 100 ?mol/L ACh which induced significantly more hyperpolarizing than control, with increased potential sensitivity of activation, V1/2(Ach) =(-52.38 ? 3.98) mV, SACh =(40 ? 4.14) mV(n= 5). Conclusion ACh increased the conductance of outwardly rectifying K + channel in a voltage dependent manner and caused a hyperpolarizing shift of activation potential. Effect of ACh was to hyperpolarize the membrane potential of OHCs from rest.

Objective To investigate the expression of NAP in patients with acute myeloid leukemia (AML). Methods Expression of NAP of peripheral blood was assessed by flow cytometry. The serum levels of G-CSF, NE and IL-18 in 53 patients with AML and 50 healthy controls were examined by ELISA. Results The expression of NAP in AML patients [1 159(563 ~ 2 033)AB/c] was decreased than that of healthy controls [1 862 (1 345 ~ 2 382)AB/c], (P < 0.05). The serum levels of G-CSF, NE and IL-8 from AML patients [34.36 (25.17 ~ 47.24) ng/L, 397.56 (324.63 ~ 563.84) pg/mL, 585.34 (403.61 ~ 866.54) pg/mL] were significantly lower than those of healthy controls[54.52(46.27 ~ 77.14) ng/L, 689.74(496.78 ~ 899.14) pg/mL, 832.36(625.17 ~ 998.24) pg/mL] (P < 0.05). The expression of NAP in AML patients was positively correlated with the serum level of G-CSF (r = 0.621, P = 0.007). Conclusions The decreased expression of NAP, G-CSF, NE and IL-8 in the peripheral blood with AML could speculated that neutrophils immune function in patients with AML was restrained.

Objective To evaluate the effectiveness of free highly active antiretroviral therapy (HAART) in adult infected with human immunodeficiency virus (HIV)/ acquired immune deficiency syndrome (AIDS) patients in Dehong area. Methods Clinical data of 1039 adult HIV/AIDS patients from five counties/cities in Dehong area who initiated HAART during the period from July 1st 2004 to June 30th 2008 were retrospectively analyzed to examine their virological and immunological responses to HAART. Data were analyzed by Chi-squared test or F test. Results Among the 1039 HIV/AIDS cases, 611 were males and 428 were females. The mean age was (37.0±9.9) years and the mean treatment duration was (22. 41 ± 12. 69) months. Complete viral suppression (HIV viral load＜50 copy/mL) was achieved in 781 cases (75. 17%). The percentage of patients achieving complete viral suppression rates were 76.95%, 76.49%, 70.65% and 77. 73% in patients treated for 6-12,13-24, 25-36 and more than 37 months, respectively (x2=8.646, P=0.194). The meanCD4+ T cell counts were (164.93±118.05) × 106/L at baseline, and (330.85±201.73) × 106/L, (356.24±205.49) × 106/L, (434.53±250.65) × 106/L and (396.31±202.62) × 106/L in patients treated for 6- 12, 13-24, 25-36 and more than 37 months, respectively. CD4- T cell counts were significantly different in patients treated for 6-12 and 13-24 months (F= 19. 423 , P＜0. 01). Successful immune reconstitution was achieved in 927 ( 90.88 % ) cases. Seven hundred and seventeen (70.29% ) cases achieved both virological suppression and immunological reconstitution with HAART, whereas 40 cases (3. 92%) failed to achieve both virological and immunological responses. Conclusion HIV/AIDS patients in Dehong area show good virological and immunological responses to HAART.

OBJECTIVETo study potassium currents in isolated Deiters' cells of guinea pig cochlea and explore possible function of potassium current in Deiters' cell.

METHODSThe whole cell patch clamp recording technique was used to study potassium currents of Deiters' cells in normal external solution and solutions with different K(+) concentrations. We also studied the effects on reversal potentials and outward potassium currents.

RESULTSIsolated Deiters' cells possess voltage dependent, outwardly rectifying ion channels, which are K(+) selective. 50 mmol/L K(+) and 150 mmol/L K(+) in external solution reduced I(K-max) from (10.06 +/- 2.2) nA (n = 13) in normal external solution to (6.43 +/- 1.67) nA (n = 6, P < 0.05) and (5.49 +/- 1.33) nA (n = 6, P < 0.05), respectively. While the amplitude of tailcurrents decreased from (468.76 +/- 61.76) pA in 5 mmol K(+) external solution to (224.74 +/- 35.89) pA (P < 0.05) in 50 mmol/L K(+) and to (-911.59 +/- 78.17) pA (P < 0.01) in 150 mmol/L K(+) external solution.

CONCLUSIONSOutwardly rectifying potassium in Deiters' cells could buffer extracellular K(+) in the small space between Deiters' cells and outer hair cells or neural fibers and participate in the diffusion of K(+) from endolymph to perilymph.

Animals ; Cochlea ; cytology ; drug effects ; physiology ; Dose-Response Relationship, Drug ; Guinea Pigs ; Membrane Potentials ; drug effects ; physiology ; Patch-Clamp Techniques ; Potassium ; pharmacology ; Potassium Channels ; physiology

Background Decline in cognitive function is considered a hallmark of schizophrenia,and transcranial direct current stimulation(tDCS)has developed as a promising tool for cognitive enhancement.Objective To systematically evaluate the effect of tDCS on improving cognitive functioning in patients with schizophrenia,so as to provide references for clinical intervention of cognitive function in patients with schizophrenia.Methods A computer-based systematic search was conducted on September 12,2023 through CNKI,Wanfang,VIP,PubMed,CINAHL and PsycINFO databases,and randomized controlled trials relevant to the efficacy of tDCS for management of cognitive function in patients with schizophrenia were collected.The risk of bias was assessed using the Cochrane Risk of Bias Tool for Randomized Trials(RoB 2.0).Results A total of 17 articles were included,including 953 patients with schizophrenia.TDCS has an improvement effect on cognitive functions such as information processing speed,attention,working memory,executive function and learning ability in patients with schizophrenia.Conclusion tDCS may have an effect on improving cognitive deficits in patients with schizophrenia.

Objective To understand the current status of receiving no methadone maintenance treatment (MMT) and influencing factors in HIV infected injecting drug users (IDUs) in Dehong Dai and Jingpo autonomous prefectures,Yunnan province.Methods Data of survival of IDUs with AIDS in Dehong were collected from "Chinese National Comprehensive HIV/AIDS and Care Information System" in December,2014.Results There were 987 IDUs who should receive MMT,the majority of them were males (94.6％,934/987),aged 35-44 years (53.0％,523/987) and farmers (77.2％,762/987).Among the 987 IDUs,60.2％ (592/987) received no MMT.Multivariate logistic regression analysis showed that being female (OR=2.66,95％CI:1.21-5.87),in Jingpo ethnic group (OR=3.05,95％CI:1.97-4.71) were the major risk factors for receiving no MMT;not being farmers (OR=0.46,95％CI:0.31-0.70),in Dai ethnic group (OR=0.53,95％CI:0.36-0.79),diagnosed HIV infection history ≥ 10 years (OR=0.60,95％CI:0.45-0.81) were the major protective factors for receiving no MMT.The reasons for receiving no MMT included long distance journey (289,48.8％),fear of exposure (124,20.9％),poor daily medication compliance (59,10.0％),fear of side effects (47,7.9％),others (73,12.3％).Conclusions The proportion of receiving no MMT in IDUs with AIDS in Dehong was high.Being female and farmer,in Jingpo ethnic group,low educational level,short diagnosed HIV infection history were influencing factors for receiving no MMT.The effective intervention measures should be taken to further improve MMT coverage according to the different characteristics of the patients.

Objective:To determine the baseline HIV viral load and demographic characteristics of newly diagnosed HIV cases from 2015 to 2017 in Dehong prefecture and provide a scientific basis for improving HIV prevention and treatment.Methods:All newly reported HIV-infected cases from 2015 to 2017 in Dehong prefecture were included in this study. Viral load, CD 4+ T cell counts and the demographic characteristics were collected and retrospectively analyzed. Results:From 2015 to 2017, a total of 1 157 newly diagnosed HIV cases were reported in Dehong prefecture, of which 1 057 cases were tested for viral load; 64.9% were males, 59.5% aged between 25 and 49, 51.9% were married, 48.2% were Han ethnicity, 48.2% had middle school (or beyond) training and 85% were heterosexual. The median pre-treatment viral load of newly diagnosed HIV-infected individuals was 8 200 (IQR: 1 900-44 000) copies/ml. The proportion of cases with viral load between 10 3-10 5copies/ml was the highest, accounted for 65.7% (694/1 057). There were 5.2% (55/1 057) whose viral load was below the detecting limit and 24 had CD 4+ T cell counts over 500 cells/μl. Conclusions:Baseline viral load levels before treatment vary among HIV-infected individuals. In view of the impact of viral load at baseline on the effectiveness of diagnosis and treatment, it is necessary to perform viral load testing before treatment, and the result can assist in the formulation of a more personalized and effective treatment regimen.

Objective To explore the distribution of HIV subtype in newly detected people living with HIV from January to November,2015 in Dehong Dai and Jingpo Autonomous Prefecture,Yunnan province.Methods DNA extraction,reverse transcription polymerase chain reaction (RT-PCR) for gag,env,and pol amplification and amplification product sequencing were conducted by using plasmas of newly detected HIV-infected persons.The subtypes were confirmed by analyzing the sequences of 3 genes.Results A total of 963 HIV infection cases were reported during this period,the HIV subtype was confirmed in 499 cases.Unique recombinant form (URF) was the most common subtype (27.1％,135/499),followed by C (26.7％,133/499),CRF01_AE (19.2％,96/499) and others.URF included 4 kinds of combination,of which combination of subtype B and C was most common.HIV subtype distribution differed between the Chinese HIV infection cases and the Burmese HIV infection cases,the proportion of B and C combination was higher in the Chinese cases.Transmission route was the only factor influencing H1V subtype distribution.Conclusions HIV subtype distribution in Dehong was complex.URF was predominant.The HIV subtype distribution differed between Chinese and Burmese under different transmission route.