Objective: To observe the effects of salvia miltiorrhizae compound (SMC) on serum endothelin (ET), TXB_2 and PGI_2 levels in patients undergoing CPB. Method: Twenty patients with congenital heart disease were randomly divided into control group (group Ⅰ) and treated group (group Ⅱ). SMC (200mg/kg) were given intravenously in group Ⅱ before operation and during rewarming, in group Ⅰ eqivalent volumes of normal saline were administered at the same time point. The serum ET,TXB_2 and PGI_2 levels were measured at preoperation (T_0) 5min (T_1), and 30min (T_2) following CPB, 10min (T_3), 30min (T_4) and 60min (T_5) following reperfusion, 24h (T_6) after operation. Result: In both groups,the serum ET level were increased significantly during reperfusion, but much less in group Ⅱ than that in group Ⅰ at T_4, T_5 and T_6(P

The platelets in the human mixed thrombus,hyaline thrombus antemortemand postmrtem skin incision wounds were detected by the PAP-immunohisto-chemical technique using monoclonal antibodies against the human platelet mem-brane glycoprotein(GPIb,GPIIIa).The positive reaction were observed inthe mixed thrombus,hyaline thrombus and in all the antrmortem skin incisionwounds,but not in the postmortem skin wounds.The significance of theapplication of this technique in the forensic medicine practice was discussed.

Objective:To optimize the water extraction and alcohol precipitation technology of Xuanfei Zhike granule .Methods:Orthogonal test was used to investigate the effects of adding water , decocting time and boiling time on the water extraction , and the effects of relative density , alcohol precipitation concentration and alcohol precipitation time on the alcohol precipitation technology by taking comprehensive score including the amount of hesperidin , the amount of tectoridin and the yield of dry cream as the indices .Re-sults:The preferred water extraction technology was as follows: added 10 times water and extracted 1.5 h firstly, and then added 8 times water and extracted twice with 0.5 h for each.The preferred alcohol precipitation technology was as follows:concentrated the wa-ter extraction to a relative density of 1.05 (measured at 60℃), slowly added 95％ethanol to 80％alcohol solution and stored 18 h at low temperature .Conclusion:The optimal water extraction and alcohol precipitation technology is stable and feasible , which can pro-vide reference for the standardized production of Xuanfei Zhike granule .

Objective:To survey bone age, vitamin A, vitamin D and IGF-1 levels among stunted children, and to explore the clinical values.Methods:The experimental group was composed of 200 stunted children who visited the growth retardation clinic of Jiangning Hospital Affiliated to Nanjing Medical University between October 2017 and October 2019.The control group consisted of 200 children of normal height during the same period.The differences of developmental level, the qualified rates of serum vitamin A and vitamin D, and the number of the children whose serum IGF-1 at or above the median and their corresponding measurements were compared between the two groups.Results:Totally, 26% of the experimental group fell behind normal children by two years in their bone ages, as compared with 12% of control group.The differences in developmental levels of bone ages between the two groups were statistically significant( χ2=12.74, t=5.42、7.92, P<0.01). Compared with the control group, the experimental group had obviously lower rates of vitamin A and vitamin D levels( χ2=26.85、13.65, t=8.45、12.47, P<0.01). A total of 88 children (44%) of the experimental group had serum IGF-1 levels at or above the median, as compared with 138 children (69%) of the control group( χ2=25.43, t=32.31, P<0.01). Conclusion:This finding supports the potential use of the bone age, vitamin A and D status and IGF-1 levels in growth retardation screening among children.