Since December 2019,the rapid spread of coronavirus disease 2019(COVID-19)poses a serious threat to global public health security.Obstructive sleep apnea hypopnea syndrome(OSAHS)is commonly occurred in patients with COVID-19,however,due to the lack of understand-ing of their comorbidities,the damage of OSAHS is often underestimated or even ignored.OSAHS has not been identified as an independent risk factor that increases the possibility of severe acute respirato-ry syndrome coronavirus 2(SARS-CoV-2)infection and aggravates the disease.In addition,the un-derstanding of traditional Chinese medicine for the prevention and treatment of novel coronavirus pneumonia complicated with OSAHS at home and abroad is not profound,and related studies are also few.The purpose of this article is to improve clinicians'knowledge and understanding of COVID-19 combined with OSAHS,which may provide some guidance in dealing with this disease.

Since December 2019,the rapid spread of coronavirus disease 2019(COVID-19)poses a serious threat to global public health security.Obstructive sleep apnea hypopnea syndrome(OSAHS)is commonly occurred in patients with COVID-19,however,due to the lack of understand-ing of their comorbidities,the damage of OSAHS is often underestimated or even ignored.OSAHS has not been identified as an independent risk factor that increases the possibility of severe acute respirato-ry syndrome coronavirus 2(SARS-CoV-2)infection and aggravates the disease.In addition,the un-derstanding of traditional Chinese medicine for the prevention and treatment of novel coronavirus pneumonia complicated with OSAHS at home and abroad is not profound,and related studies are also few.The purpose of this article is to improve clinicians'knowledge and understanding of COVID-19 combined with OSAHS,which may provide some guidance in dealing with this disease.

Objective To investigate the advantage of neuronavigation assisted microsurgical resection of gliomas in eloquent areas of brain. Methods The clinical data of 99 patients with gliomas in eloquent areas of brain and underwent microsurgery in the First Affiliated Hospital of Sun Yat-sen University from January 2012 to December 2017 were analyzed retrospectively. All patients were divided into two groups: neuronavigator-guided microsurgery group (neuronavigation group,46 cases) and non-neuronavigator-guided microsurgery group (non-neuronavigation group,53 cases). The neuronavigation group received neuronavigation assisted microsurgery, while the non-neuronavigation group received general microsurgery. MRI examination, Karnofsky performance scale (KPS) score and symptomatic improvement rate were used to analyze the extent of resection and postoperative function. Results The total resection rate of neuronavigation group was 91.3% (42/46). Comparing with 73.6% (39/53) total resection rate in non-neuronavigation group,the difference of total resection rate was statistically significant (Z=-2.343,P＜0.05). The postoperative symptoms improved both in neuronavigation group and non-neuronavigation group,and the KPS score at discharge were higher than that before operation (P＜0.05). No aggravation of symptoms and signs or new complications occurred in all patients except one case of postoperative muscle strength decline in non-neuronavigation group after the surgery. According to tumor size,the total resection rate of neuronavigation group and non-neuronavigation group among patients whose maximum size of tumor≥3cm were 89.5%(34/38) and 72.5%(37/51) respectively. The difference of total resection rate was statistically significant between these two groups (Z=-2.040,P＜0.05). Conclusion Neuronavigation assisted microsurgical resection of gliomas in brain eloquent areas can improve the rate of total resection and postoperative quality of life without increase in postoperative complications.

OBJECTIVE To explore the effect and mechanism of Kangaoheji(KAHJ)in the treatment of acute lung injury(ALI)in mice,and provide a rationale for its possible use as a drug to alleviate symptoms following coronavirus disease 2019(COV-ID-19)infection.METHODS Network pharmacology was carried out to predict the main active components and potential targets of KAHJ on ALI.C57BL/6J mice were randomly divided into Control group,LPS group and LPS+KAHJ group.LPS+KAHJ group was gavaged with KAHJ(4.76 g·kg-1·d-1,8.8 mL·kg-1·d-1)and the rest of the groups were gavaged with saline(8.8 mL·kg-1·d-1).LPS(5 mg·kg-1)was injected intraperitoneally to induce an acute inflammation model after 14 d.The ser-um and lung tissues of mice were collected,and the pathological changes in lung tissues were observed via histopathology.Western blot,Real-time PCR,Enzyme-Linked immunosorbent assay(ELISA)and immunohistochemistry(IHC)were used to assess the a-meliorative effect of KAHJ on ALI.RESULTS The result showed that 70 core target genes of KAHJ on ALI were primarily implicated in multiple signaling pathways involving MAPK signaling pathway,NF-κB signaling pathway,apoptosis,and Ras signaling pathway.Furthermore,we found that KAHJ ameliorated inflammation and apoptosis in ALI,thereby reducing lung damage and pulmo-nary edema and inhibiting pulmonary fibrosis.Additionally,KAHJ inhibited the phosphorylation of p38 MAPK and NF-κB,and up-regulated the ACE2/Ang1-7/Mas axis.CONCLUSION KAHJ might relieve acute lung injury through p38 MAPK/NF-κB signaling pathway and ACE2/Ang1-7/Mas axis,which offers complementary and alternative treatment options for COVID-19.

Portal vein thrombosis is a common complication after esophagogastric devascularization and splenectomy (EDS), with concealment and potential risks, which limited the therapeutic effect. The patients undergoing EDS are often accompanied by severe liver cirrhosis, whose abnormal hemostasis poses a dilemma for antithrombotic therapy. This article reviewed on the research progress of hemostasis of these patients, and risk factors, mechanism, prevention, therapy of post-EDS portal vein thrombosis.

Objective:To establish a real-time fluorescent quantitative TaqMan reverse transcription polymerase chain reaction (Real-time RT-PCR) detection method for Wuxiang virus (WUXV).Methods:All gene sequences of WUXV were downloaded from GenBank, and multi-sequence alignment analysis was performed using Mega-X. Primers and probes designed for the highly conservative region of S-segment genes were selected to evaluate the specificity, sensitivity and stability of detection reactions.Results:The established method can specifically detect WUXV and does not cross-react with various arboviruses. The lowest detection limit was 1 pfu/ml. The inter-batch variation coefficients of repeated detection cycle threshold ( Ct) of the same sample were all less than 1.00%. TaqMan RT-PCR was used to detect 30 batches of sandflies nucleic acid samples, and 7 of them showed positive amplification curve of WUXV. Conclusions:TaqMan RT-PCR with high sensitivity, specificity and repeatability has been successfully established, which can be used to screen large quantities of samples of WUXV.

Objective:To explore the effects of early wet compress with traditional Chinese medicine Ru-Yi-Jin-Huang-San on drug extravasation skin injury.Methods:Totally 21 patients with skin injuries caused by extravasation of different drugs from the First People's Hospital of Lianyungang and General Hospital of Eastern Theater Command between January 2017 and December 2019 were selected by convenient sampling. They received local wet compress with Ru-Yi-Jin-Huang-San according to the redness and swelling area, which was replaced once a day until the pain and swelling were ameliorated and the wound healed. Patients with irreversible tissue necrosis underwent debridement, anti-infection, negative pressure wound treatment or wet therapy according to the principles of wound care until the wound healed. The pain score, redness area, healing time and healing outcome were compared before and after wet compress, between upper and lower limbs, between wet compress within 24 hours and wet compress after 24 hours, and between patients with and without drug extravasation.Results:After wet compress with Ru-Yi-Jin-Huang-San for 2-13 days, the skin injury of 18 patients who received wet compress within 2-24 hours after extravasation healed completely, and the healing time was (6.44±2.99) d; 3 cases formed wounds, which healed after local debridement, anti-infection, wet therapy and negative pressure therapy, with a healing time of 67 (28, 154) d. There were statistically significant difference between the pain score before wet compress and those from day 1 to day 7 after wet compress ( P<0.05) ; there were statistically significant differences between the redness and swelling area before wet compress and those from day 1 to day 5 after wet compress ( P<0.05) ; there was no statistically significant difference in the redness and swelling area between day 6 and day 7 ( P>0.05) . There were no statistically significant differences in the pain score and the redness and swelling area between the upper and lower extremities before wet compress ( P>0.05) . 3 days after wet compress, the pain score of the upper extremities was lower than that of the lower extremities, and the difference was statistically significant ( P<0.05) , but there was no statistically significant difference in the redness and swelling area between the lower extremities and the upper extremities ( P>0.05) . Before wet compress, there was no statistically significant difference in the pain score and the redness and swelling area between the groups with different time of wet compress ( P>0.05) . 3 days after wet compress, the pain score of patients who received wet compress within 24 hours was lower than that of patients who received wet compress after 24 hours ( P>0.05) . The healing time of patients who received wet compress within 24 hours was shorter than that of patients who received wet compress after 24 hours, and the difference between the two groups was statistically significant ( P<0.05) . Before wet compress, there were no significant differences in pain score and the redness and swelling area between the groups with extravasation of different drugs ( P>0.05) . 3 days after wet compress, there were no statistically significant differences in the pain score and the redness and swelling area within the antibiotic extravasation group ( P>0.05) . Conclusions:Early wet compress with Ru-Yi-Jin-Huang-San is safe and effective for drug extravasation skin injury. The sooner the wet compress, the better the effect is.

Objective:To investigate the impact of neuroendocrine function on postoperative complications and nutritional status in gastric cancer patients.Methods:Clinical data of 102 gastric cancer patients who underwent radical gastrectomy at the Department of General Surgery, First Affiliated Hospital of Soochow University, from Aug 2021 to Jun 2022 were retrospectively analyzed.Results:Among the 102 gastric cancer patients, 18 (17.6%) suffered from postoperative complications. Univariate analysis indicated that age, BMI, preoperative plasma ghrelin concentration, and preoperative hemoglobin levels were associated with early postoperative complications following radical gastrectomy. Multivariate analysis revealed that age, BMI, and preoperative plasma ghrelin concentration ( P<0.05) were independent risk factors for postoperative complications in gastric cancer patients. Differential analysis of ghrelin concentration demonstrated correlations with hemoglobin levels, skeletal muscle index, albumin, and creatinine, and a positive correlation with the skeletal muscle index. Conclusions:Reduced preoperative neuroendocrine hormone ghrelin concentration is an independent risk factor for postoperative complications in gastric cancer patients. Ghrelin concentration is correlated with the skeletal muscle index in these patients.

Lateral flow immunochromatography assay is a new instantaneous detection technology that employs a chromatographic membrane and labeling materials for detection.This detection technology is convenient,fast,and inexpensive,and is therefore widely used in a number of different fields,such as biomedicine,disease detection,food safety,environmental protection,and so on.Traditional lateral flow immunochromatography assay relied on visual observation and provided only qualitative or semi-quantitative results.By utilizing various types of markers and sensitive detection devices,lateral flow immunochromatography assay enables quantitative and multi-component detection of the analytes.The research progress on the lateral flow immunoassay detection system and its current applications in the context of recognition elements,labeling materials,and detection instruments were reviewed in this paper.

HBeAg seroconversion refers to the disappearance of HBeAg and the appearance of anti-HBe in chronic hepatitis B (CHB) patients with positive HBeAg in the past. HBeAg seroconversion marks the reductions in viral replication, immune tolerance, and liver inflammation and is an important monitoring indicator for evaluating disease conditions and the effect of CHB antiviral therapy, and it also indicates the endpoint of satisfactory treatment. Exploring the influencing factors for HBeAg seroconversion is of great significance to the selection of treatment regimens and the prognostic evaluation of CHB patients. This article mainly elaborates on the association of HBV with HBeAg seroconversion in CHB patients from the aspects of virological factors, host genetic factors, drug factors, and immunological factors.