As Chinese medicine do have advantages and disadvantages in disease prevention, we should have a good cognition on this and play out its advantages and make up its disadvantage in the practical work, so as to create a unique Chinese preventive medicine. Thus in this way, Chinese preventive medicine will be further developed and have better service for society.

An electrophoretic karyotype of Acanthamoeba polyphaga has been preliminarily analysed by means of pulse field gel electrophoresis (PFGE).Ten chromosomal DNA bands are distinguishable on the gel.Using yeast (Saccharomyces cerevisiae) chromosomal DNA as size standard,we estimate the size of the chromosomes to be between about 200 kilobase pairs (kb) and 2 megabase pairs (mb).

Chimeric antigen receptor T lymphocyte immunological therapy (CAR-T)is a new oncologic immunotherapy in recent years,which has been widely used in patients with leukemia and lymphoma and achieved a certain effect.Neuroblastoma is one of the most common extracranial solid tumors in children and over 50％ of patients have metastatic recurrence when first diagnosed.The prognosis for the high-risk neuroblastoma remains poor at the moment and it's time to look for new treatments.In this review,we summarize the basic structure and development of CAR-T,discuss the principles and risks of CAR-T in the treatment of neuroblastoma,and give an outlook to CAR-T in the treatment of solid tumors.

The ketogenic diet (KD) is an effective treatment for refractory epilepsy,including some inflammation-induced epileptic encephalopathies,and the mechanism of it is complicated.KD,through multiple targets,plays the role to inhibit pathogens,control inflammation and regulate immune function.In experiments,KD provides an anti-inflammatory effect in some rat models of fever.It has been shown that,KD decreases peripheral edema,attenuates thermal nociception and reduce arachidomic acid level in circulation.KD blocks Nod-like receptor protein 3 (NLRP3),nuclear factor-kappa B (NF-KB),oxygen free radicals,and improves Peroxisome proliferator-actived Receptor(PPAR) γlevel,to reach the effect of anti-inflammatory.This review focuses on ketogenic diet anti-inflammatory mechanism and the recent advances in clinical application.

Objective:The efficacy and toxicity of anticancer drugs differ in different tumor patients. It could not meet the requirements of optimization of tumor treatment if only under the guidance of clinical and pathologic characteristics. More and more attention is focused on individualized therapy based on individual gene profiles. It has been identified that gene polymorphisms are responsible for different outcomes of individuals. Gene polymorphisms, including sequence polymorphism and single nucleotide polymorphism, will decrease the clinical efficacy of chemotherapeutic drugs or increase adverse reactions by influencing the expression or activity of the corresponding proteins. In recent years several gene polymorphisms related to drug metabolism, transportation, or inactivation may affect the outcomes of chemotherapy and the frequency of adverse reactions. Identification of the polymorphism genes is important for individual therapy for tumor patients.

Objective To investigate the effect of Zhubei Dingchuan pill on clinical symptoms and inflammatory mediators in serum and induced sputum of patients with chronic bronchitis. Methods 80 cases of patients with chronic bronchitis treated in the Department of Respiration in our hospital from January 2016 to January 2017 were selected and were randomly divided into the control group and the observation group, with 40 cases in each group. The control group was given symptomatic support treatment and the observation group was additionally given Zhubei Dingchuan pills on the basis of the control group. The changes of inflammatory mediators levels in serum and induced sputum were measured before and after treatment between the two groups, and the clinical efficacy was observed in the two groups. Results After treatment, the levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and high-sensitivity C reactive protein (hs-CRP) in serum or induced sputum in the observation group were significantly lower than those in the control group (P<0.05). The effective rate of treatment in the observation group was significantly higher than that in the control group (95.0％ vs. 82.5％) (P<0.05). Conclusion Zhubei Dingchuan pill can effectively relieve the clinical symptoms in patients with chronic bronchitis, and its pharmacological mechanism is related to the decrease of levels serum and airway inflammatory mediators.

AIM:To investigate whether trichostatin A ( TSA) , a new revulsant ,can induce mouse mesenchy-mal stem cells to differentiate into insulin-secreting cells and to explore the appropriate concentration of TSA .METHODS:The mesenchymal stem cell line from C57BL/6 mice was cultured in vitro and divided into 5 groups before treated with dif-ferent concentrations of TSA , ( group A:DMSO;group B~E:treated with 25 nmol/L, 50 nmol/L, 100 nmol/L and 200 nmol/L of TSA, respectively).After exposed to different cultured media for 10 d during the 2 stages, the cells were detec-ted by the following methods:the insulin-secreting cells in each group were identified by dithizone staining and the results were calculated with immunohistochemical half quantitative analysis .The insulin secreted by insulin-secreting cells in each group was identified by immunofluorescence , and the mean fluorescence intensity of insulin was compared .The content of insulin in each group was quantified by ELISA .The appropriate concentration of TSA was determined according to the above results .RESULTS:TSA treatment for 10 d promoted the mouse bone marrow mesenchymal stem cells to differenti-ate into insulin-secreting cells which produced insulin .The immunohistochemistry and immunofluorescence imaging analysis of insulin-secreting cells showed that the insulin staining positive area , positive ratio , total density of insulin expression and mean fluorescence intensity of insulin in group B were significantly higher than those in the other TSA -treated groups .When the concentrations of TSA gradually increased , the content of insulin reduced accordingly .The content of insulin in group B was significantly higher than that in the other TSA-treated groups .CONCLUSION:TSA treatment for 10 d promotes bone marrow mesenchymal stem cells from C57BL/6 mice to differentiate into insulin-secreting cells and the appropriate concen-tration of TSA is 25 nmol/L.

Objective]To summarize Professor Wang’s clinical thoughts and experience of herb pairs in treating“cough caused by wind”. [Methods]To collect and analyse the theory of Wang’s experience of herb pairs in treating“cough caused by wind”by studying from him for a long time. [Results] Professor considered that“cough caused by wind”is a kind of disease characterized by nature of “wind”, which is mainly cough, with wind syndrome unique performance. It is similar to airway hyperresponsiveness diseases,including modern medicine on the infection cough, cough variant asthma, gastroesophageal reflux cough, upper airway cough syndrome, eosinophilic bronchitis, allergic cough, etc, which should be treated by the way of “dispelling wind ventilating lung and relieving cough ”. According to different wind cough sydromes, choose different herb couples.“Feixingcao-Zicao-Xiancao”,“Jiegeng-Shegan”,“Zhipipaye -Zhiziwan -Zhidonghua”,“Zhimahuang -Suye”,“Xinreng -Zhebei”,“Shengbaishao -Shenggancao”are the most commonly used herb pairs in practice.[Conclusion] Professor’s experience of herb pairs in treating“cough caused by wind”is well worth learning for it always gets outstanding effect.

Background and purpose: Alternative splicing is an important regulation mechanism of gene expression. Aberrant alternative splicing is associated with dysregulation of the cell cycle, activation of oncogenes and inactivation of the tumor suppressor genes. Thus, it is closely correlated with the pathogenesis and progression of various tumors. DNA methylation is an important part of epigenetic phenomena. Aberrant methylation of the gene promoter can result in gene silencing. Hypermethylation of tumor suppressor genes and DNA repair genes correlates with the onset of many different cancers. Additionally, DNA methylation acts as a pivotal factor for alternative splicing. Aberrant methylation disrupts the stabilization of the alternative splicing. This study investigated the promoter methylation and expression of RNA binding protein, fox-1 homolog 1 (RBFOX1) gene in esophageal squamous cell carcinoma (ESCC), and to elucidate its role in ESCC. Methods: MassARRAY approach and RT-PCR were used respectively to examine the methylation level of RBFOX1 gene and its expression at mRNA level in tumors and corresponding adjacent normal tissues. The correlation between methylation level and clinicopathological features was analyzed. Results:RBFOX1 methylation level and mRNA expression in tumor tissues were signiifcantly lower than those in corresponding adjacent normal tissues (41.8% vs 68.3%, P<0.01). No significant correlation was observed between methylation level and clinicopathological features. The cut-off (33.6%) was calculated as the mean of the normal samples to which we applied 2.5 SD. According to the cut-off value, the object of the study was divided into two groups. The methylation level lower than the cut-off was deifned as group 1;methylation level higher than the cut-off was deifned as group 2. The 5-year overall survival rates of the two groups were 57.0%and 35.7%, respectively (P=0.06);5-year progression-free survival rates were 48.7%and 28.9%, respectively (P=0.03). However, the multivariate analysis results indicated that TNM stage was the independent factor of prognosis.Conclusion: The methylation level and mRNA expression of RBFOX1 in tumor specimens are signiifcantly lower than those in corresponding adjacent normal tissues. The methylation level of the RBFOX1 promoter is not an independent factor of prognosis.

Objective To investigate the distribution and clinical significance of D‐Dimer positive patients .Methods 1 003 D‐di‐mer positive patients were enrolled in the study ,which were measured by latex enhanced immune turbidimetry .Results The total positive rate of ICU ,cardiology ,respiratory medicine ,orthopedics ,general surgery ,liver disease ,neurosurgery ,obstetrics and gyne‐cology ,oncology departments was 44 .1% .The numbers of D‐dimer positive patients with diffuse intravascular coagulation ,deep vein thrombosis ,pulmonary embolism ,heart cerebrovascular disease ,liver disease ,malignant tumor were 86 ,34 ,26 ,24 ,18 and 12 , respectively .Conclusion The determination of plasma D‐dimer could be used in thrombotic disease prevention and monitoring .