The important difference between post-graduate and undergraduate education is to train their reseach ability,which is very important especially to the post-graduate students of basic medicine who will be researchers.The problem in training research ability of post-graduate was studied in this paper and solution plan was put forward.

Objective To observe the effect of Montelukast (MTL)on the airway smooth muscle(ASM)iso-lated from asthmatic guinea pigs.Methods The bronchus of asthmatic guinea pigs were made into trachea spiral. These bronchus were divided into 6 groups:MTL group,MTL +Nifedipine group,MTL +Glibenclamide,MTL +Prop-ranolol group,MTL +Atropine group and control group.The effect of different concentrations of MTL on the ASMin the resting tension was observed and the ASMrelaxation pigs were compared among the groups.Results MTL in 1 0 -7.5 -1 0 -3.0 mol/L had diastolic effect on the asthmatic guinea pigs ASM[(0.31 ±0.1 2)% -(99.61 ±0.40)%],and compared with the control group,there were significant differences[0 -(1 2.77 ±0.33)%,all P <0.05].In the resting tension of asthmatic guinea pig ASM[(1 1 .48 ±1 .63)% -(94.03 ±1 .87)%],the diastolic function was remarkably improved at 1 0 -6.0 -1 0 -3.5 mol/L of MTL in MTL +Nifedipine group,and the differences among the groups had statis-tical significance (all P <0.05).The diastolic function was antagonism at 1 0 -7.5 -1 0 -6.5 mol/L of MTL in MTL +Pro-pranolol group [(0.1 6 ±0.07)% -(2.73 ±0.84)%]and the differences among the groups had statistical signifi-cance (all P <0.05).The diastolic function had no significant difference between 1 0 -8.0 -1 0 -3.0 mol/L of MTL in MTL +Atropine group and MTL +Glibenclamide group (all P >0.05).Conclusions It has diastolic function for MTL on the isolated asthmatic guinea pigs ASMin the resting tension.The effect may be related with inhibitor of Ca2 + inter-nal flow and the competitive exciting β2 receptor.

Objective To observe the effect and mechanism of Ligustrazine ( LIG ) on the Airway smooth muscle( ASM) of isolated guinea pigs. Methods The normal guinea pig bronchus were made into tra-chea spiral,and randomly divided into LIG group, LIG+Propranolol group, LIG+Atropine, LIG+Nifedipine group,LIG+Glibenclamide group and the control group. The effect of different concentrations of LIG on the ASM was respectively observed in the resting tension and the high tension caused by histamine,and the ASM re-laxation rate were compared between each groups. Results (1)It had diastolic function for LIG on the isolated guinea pigs ASM in the resting tension and the high tension caused by histamine. (2)In the resting tension,com-pared with the group LIG,the diastolic function was antagonism by the concentration of LIG 10-7 ~10-6. 5 mol/L in the group LIG+Propranolo (P<0. 05). The diastolic function had no significant difference by the concentra-tion of LIG 10 -7.5 ~10 -3 mol/L in the group LIG+atropine and LIG+Glibenclamide(P>0. 05). The diastolic function was improved remarkably by the concentration of LIG 10 -6 ~10 -4 mol/L,in the group LIG+Nifedip-ine (P<0. 05). (3)In the high tension caused by histamine,compared with the group LIG,the diastolic function was antagonism obviously by the concentration of LIG 10 -6. 5 ~ 10 -5 mol/L in group LIG+Propranolol ( P<0. 05);The diastolic function had no significant difference by the concentration of LIG 10 -8 ~10 -3 mol/L in group LIG+atropine,and LIG+Glibenclamide (P>0. 05);The diastolic function was improved remarkably by the concentration of LIG 10 -6.5 ~10 -5 mol/L in group LIG+Nifedipine (P<0. 05). Conclusion It has dias-tolic function for LIG on the isolated guinea pigs ASM in the resting tension and the high tension caused by his-tamine. The effect and mechanism may be related with the competitive exciting beta 2 receptor and the inhibitor of Ca2+ internal flow. It provides a theoretical basis for the clinical application of LIG in treating asthma.

Objective To evaluate the clinical value of serum amyloid protein(SAA),C-reactive protein (CPR)and SAA/CRP ratio in early diagnosis of influenza A in children. Method SAA,CRP,SAA/CRP ratio and serum white blood cell(WBC)count were measured in 417 children. One hundred and thirty-nine children were diagnosed with Influenza A and were enrolled in the influenza A group ,and the other 278 healthy children were enrolled in the control group from April 2016 to June 2016. The values of the screened biomarkers for diagno-sis of influenza A were assessed by ROC curve analysis. Results Levels of SAA,CRP and SAA/CRP ratio in the influenza A group were significantly higher than those in the control group(P<0.001,respectively). The areas un-der the ROC curve of SAA and SAA/CRP ratio for the diagnosis of influenza A were higher than 0.7(P<0.001). The optimal cut-off values of SAA and SAA/CRP ratio for the diagnosis of Influenza A were 10.500 mg/L and 6.165,respectively. The corresponding sensitivities of the optimal cut-off values for the diagnosis of influenza A were 85.5% and 55.4%,respectively,and the corresponding specificities were 96.8% and 91.4%,respectively. Conclusion Measurement of SAA and SAA/CRP ratio are valuable biomarkers for clinical in early diagnosis of in-fluenza A and SAA has a better diagnostic performance than CRP.

Objective To explore the effect of Vitamin K1(Vit K1), fresh frozen plasma (plasma) andcryoprecipitate on prothrombin time (PT), activated partial thromboplastin time (APTT), fibrinogen original(Fbg), thrombin time (TT) of newborns with different gestational ages. Methods The serum of 1,134 newbornsfrom The Third Affiliated Hospital of Guangzhou Medical University was collected from February 2009 to September2012. All newborns had been divided into four groups (according to the gestational age of 28-31+6 weeks, 32-33+6weeks, 34-36+6 weeks and gestational age≥37 weeks).The effect of various interventions (Vit K1, Vit K1+plasmaand Vit K1+cryoprecipitate) on PT, APTT, Fbg, and TT had been recorded. Results (1)The PT and APTT ofeach group with the interventions of Vit k1 were significantly improved (P < 0.05). (2)The PT, APTT, Fbg and TTof each group with the interventions of Vit k1 combined with plasma were significantly improved (P < 0.05). (3)ThePT, APTT and Fbg of each group with the interventions of Vit k1 combined with cryoprecipitate were significantlyimproved (P < 0.05). (4)With Vit k1 combined with plasma, PT and APTT were mostly improved and Fbg wasimproved mostly with Vit k1 combined with cryoprecipitate. Conclusion Vitamin K1, fresh frozen plasma andcryoprecipitate can effectively improvedin the coagulation index of newborns with different gestational ages.

Objective To select early prediction indexes related to the prognosis of severe hepatitis in ultrasonography,and evaluate its clinical value.Methods Clinical data of 269 cases of severe hepatitis patients was analyzed retrospectively.Cox proportional hazards regression model was adopted to analyze 13 possible prediction ultrasonic parameters for severe hepatitis,including thickness of the right hepatic lobe,portal vein (PV) diameter,volume of ascites,etc.After selected from these parameters,the clinical value of early prediction factor was evaluated with ROC curve.Results Multivariate analysis showed that thickness of the right hepatic lobe,portal vein (PV) diameter,opening of paraumbilical vein and bile viscosity were related to the death of severe hepatitis patients (P ＜ 0.05).The thickness of the right hepatic lobe contributed most (Wald =40.479),its sensitivity and specificity for prediction were 93.4％,82.8％ respectively(P ＜ 0.01).Conclusions Thickness of the right hepatic lobe,PV diameter,opening paraumbilical vein and bile viscosity are early prediction factors for severe hepatitis,need to be monitored in treatment,especially for thickness of the right hepatic lobe.

Objective:To investigate the effect of heat shock protein B8 (HspB8) downregulation on retinal ganglion cell (RGC) and retinal function in the mice model of optic nerve injury (ONC).Methods:Adeno-Associated Virus (AAV) 2 AAV2-shHspB8-GFP was constructed to knockdown HspB8. 66 adult male C57/BL6 mice were randomly divided into the control group, the ONC group, the AAV2-shHspB8 group, the ONC+AAV2-shHspB8 group, and the ONC+AAV2-GFP group. There were 10, 20, 16, 10 and 10 mice respectively, and both eyes were used as experimental eyes. Western blot was used to evaluate the expression of HspB8 on day 3 and 7 after ONC. By GFP immunofluorescence staining, the efficacy of AAV2-shHspB8-GFP transfer was accessed. Moreover, it was possible to identify functional and RGC survival differences between groups by optomotor response (OMR), dark adapted full-field flash electroretinogram (ff-ERG), oscillatory potentials (OPs), photopic negative response (PhNR) and retinal flat-mount RGC counting 5 days after ONC. Comparisons between two groups were made using Mann-Whitney U test, unpaired t-test, unpaired t-test with Welch’s correction, one-way ANOVA, and Bonferroni t test. Results:Compared with the control group, the expression of HSPB8 protein in the retina of mice in ONC3 group was significantly increased, and the difference was statistically significant ( F=43.63, P<0.01). Compared with the control group, the ONC group showed obviously lower visual acuity ( P<0.01), lower a-wave, b-wave, OPs, PhNR amplitude, longer b-wave latency ( P<0.05), and the survival rates of RGC in ONC3 group, ONC5 group and ONC7 group decreased in a time-dependent manner( F=384.90, P<0.01). Transfection of AAV2 efficiency was highest on 4 weeks after IVT. Besides, there was no significant differences between the control group and the AAV2-shHspB8 group on visual acuity, ff-ERG, OPs, PhNR and RGC survival ( P>0.05). In comparison of the control group, we found that RGC survival of the ONC5+AAV2-shHspB8 group was significantly elevated ( F=10.62, P<0.01). Conclusions:Expression of HspB8 on the retina can be induced by ONC. The investigation of RGC counting, visual acuity, and ff-ERG revealed that optic nerve injury destructed functionality of mice retina and resulted to RGC death ultimately. The Most crucial finding of this research is that HspB8 knockdown had a neuroprotective effect in RGC after ONC.

Objective: To investigate the activities of daily living and the use of assistive devices among disabled people demanding assistive devices in Jiading Township, Jiading District, Shanghai Municipality, so as to provide the evidence for the implementation of rehabilitation interventions and the selection of assistive devices for the disabled people living in the community. Methods: The disabled people demanding assistive devices were sampled from 17 communities in Jiading Township in January 2020. A face-to-face questionnaire survey was conducted to collect participants'age, gender, type of disability, provision and use of assistive devices. The activities of daily living were evaluated using the Activities of Daily Living Scale, and the difference in the frequency of assistive device use was compared among the disabled people with different activities of daily living. Results: A total of 377 questionnaires were allocated, and 328 valid questionnaires were recovered, with an effective recovery rate of 87.00%. The respondents included 187 men ( 57.01% ) and 141 women (42.99%), and had a mean age of ( 71.78±13.86 ) years. Among all respondents, limb disability was predominant (248 people, 75.61%), and 84.45% (277 people) were provided with assistive devices. Assessment of activities of daily living showed complete self-care in 85 respondents ( 25.91% ), functional decline in 39 respondents ( 11.89% ) and functional dysfunction in 204 respondents ( 62.20% ). The proportion of functional dysfunction was predominantly detected among disabled people at ages of 80 years and older ( 79.63% ) ( P<0.05 ). Of the disabled people with assistive devices, 139 people used the devices daily ( 50.18% ), and the proportion of daily use of assistive devices was significantly higher in people with functional dysfunctions than in those with complete self-care ( 59.33% vs. 29.41%; P<0.05 ). In addition, no need was identified as the predominant cause of non-provision of assistive devices ( 29 people, 56.86% ). Conclusions Functional dysfunction of daily living is highly prevalent among the disabled people demanding assistive devices in Jiading Township, in whom the elderly are predominant, and the proportion of assistive device use is high among people with functional dysfunctions. The provision of assistive devices recommends to be improved based on assessment of activities of daily living.

Five compounds were separated from Sambucus chinensis and identified as maslinic acid(1), 12alpha, 13-dihydroxyolean-3-oxo-28-oic acid(2), 13-hydroxyolean-3-oxo-28-oic acid (3), 3-oxo oleanolic acid (4), corosolic acid (5). Of them,compound 3 was a new compound, and compounds 1, 2, 4, and 5 were seperated from this plant for the first time.
Oleanolic Acid ; analysis ; Sambucus ; chemistry ; Triterpenes ; analysis

Objective:To investigate the effects of Krüppel-like factor 7 (KLF7) on the survival of retinal ganglion cells (RGCs) and electroretinogram (ERG) after retinal ischemia-reperfusion (RIR) injury in mice.Methods:A total of 126 male C57BL/6J mice were randomly divided into normal group, RIR group, normal-KLF7 group, normal-green fluorescent protein (GFP) group, RIR-KLF7 group and RIR-GFP group. At the age of 8 weeks, mice of normal-KLF7 group and RIR-KLF7 group were intravitreally injected 1ul of 1.0×10 12 vg/ml adeno-associated virus overexpressing KLF7 (AAV2-KLF7-GFP). Mice of normal-GFP group and RIR-GFP group were injected adeno-associated virus of AAV2-GFP with the same titer. At the age of 11 weeks, RIR injury was induced in mice of RIR group, RIR-KLF7 group and RIR-GFP group, and intraocular pressure was measured. Retinal cryosections were used to access the efficacy of virus transfection 4 weeks after AAV2-KLF7-GFP transfer. 7 days after RIR injury, RGCs' survival rate was observed and quantified by immunofluorescent staining. ERG was performed to observe the differences in amplitudes and incubation period of scotopic ERG a-, b-wave, oscillatory potentials (Ops), photopic negative responses (PhNR). Optomotor response was performed to observe the differences of visual acuity. Expression of KLF7 was detected by western blot 4 weeks after AAV2-KLF7-GFP transfer. Results:Compared with normal group, RGCs’ survival rates, amplitudes of ERG a-, b-wave, Ops, PhNR and visual acuity of mice in RIR group were decreased, and the differences were statistically significant ( t=12.860, 7.157, 5.735, 8.953, 4.744, 9.887; P<0.05). With the increase of light intensity, the amplitudes of scotopic ERG a- and b-wave were gradually increased while the incubation period was gradually shortened. Compared with RIR group, RGCs’ survival rates, amplitudes of ERG a-, b-wave, Ops, PhNR and visual acuity of mice in RIR-KLF7 group were increased, and the differences were statistically significant ( t=6.350, 3.253, 3.695, 5.825, 5.325, 4.591; P<0.05). Protein level of KLF7 was up-regulated in normal-KLF7 group than those in normal group, and the difference was statistically significant ( t=4.105, P<0.01). Conclusion:Overexpression of KLF7 can improve RGCs’ survival rates and preserve the electrophysiological function.