Objective:Grasp the status of Zhejiang entry standards, controls and calibrators in recent years and problems.Methods:Through descriptive statistics, cross-tab chi-square, and comparation of means method , we collected and sorted out relevant information about standard products, quality control products and calibrators of the Zhejiang Provincial Biomedical Special Articles Entry Centralized Supervision Platform, and analyzed the basics of standard products, quality control products and calibrators situation, degree of dependence and utilization.Results:The standard products and quality control products imported into Zhejiang province are mainly used for scientific research of biomedical device enterprises. 76.6% of imported standard products, quality control products and calibrators cannot be domestically produced. The main origin of imported standard products and quality control products is the United States, and the main origin of standard products is the United Kingdom.Conclusions:The standard products, quality control products and calibration products imported into Zhejiang province are currently lacking in domestic production capacity. It is recommended to support the research and development and production of domestic standard products, quality control products and calibration products.

OBJECTIVE: To observe the effect of exosomes secreted by lipopolysaccharides (LPS)-stimulated macrophages on hepatic stellate cell activation and migration and explore the underlying molecular mechanism. METHODS: Human monocyte THP-1 cells were induced to differentiate into macrophages using propylene glycol methyl ether acetic acid (PMA, 100 ng/mL, 24 h) followed by stimulation with LPS, and the culture supernatant of macrophages was collected for extraction of the exosomes by ultracentrifugation. The expression of miR-155-5p in the exosomes was detected using qRT-PCR. A Transwell co-culture system was used to observe the effects of the macrophage-derived exosomes on LX2 cell (a hepatic stellate cell line) proliferation, migration, oxidative stress and the expression of fibrosis biomarkers, which were also observed in LX2 cells transfected with miR-155-5p-mimics or miR-155-5p-inhibitors. Western blotting was used to detect the expressions of SOCS1 and its downstream signal pathway proteins. RESULTS: Treatment with the exosomes from LPS-stimulated macrophages significantly enhanced the proliferation and migration ability of LX2 cells and increased the levels of oxidative stress and expressions of the fibrosis markers such as type Ⅰ collagen (P < 0.05). The expression of miR-155-5p in the exosomes secreted by macrophages was significantly increased after LPS treatment (P < 0.01). LX2 cells overexpressing miR-155-5p also exhibited significantly enhanced proliferation and migration with increased oxidative stress levels and expression of type Ⅰ collagen (P < 0.05), and interference of miR-155-5p expression produced the opposite effects. Western blotting showed that miR-155-5p overexpression obviously inhibited SOCS1 expression and promoted p-Smad2/3, Smad2/3 and RhoA protein expressions in LX2 cells (P < 0.05). CONCLUSION LPS stimulation of the macrophages increases miR-155-5p expression in the exosomes to promote activation and migration and increase oxidative stress and collagen production in hepatic stellate cells.
Humans ; Hepatic Stellate Cells ; Lipopolysaccharides/pharmacology* ; Collagen Type I ; Exosomes ; Macrophages ; MicroRNAs