1.Dynamics of specific antibody and circulating antigen in serum from the mice infected with Armillifer agkistrodontis
Hao LI ; Shaohong CHEN ; Yongnian ZHANG ; Jian GUO ; Zhengshan CHANG ; Jiaxu CHEN
Chinese Journal of Zoonoses 2012;(8):807-810
In this study,adults of Armillifer agkistrodontis (A.agkistrodontis) were collected from Agkistrodon acutus,and then the eggs were separated to feed mice.In the next step,when the infection model was established,blood serum of infected mice were collected after 1,2 and 3 weeks,respectively.Furthermore,ELISA and dot- ELISA were used to detect the dynamic change of specific antibodies and circulating antigens respectively.The specific antibodies increased from 8th week,reached the top at 12th week,decreased from 16th week,and then maintain at the same level constantly.Meanwhile,the specific antibodies were typed.It is evident that IgM antibody appeared first.However,it was substitute by IgG1 after 16 weeks.Moreover,the circulating antigens have been detected in the 1st week by dot-ELISA.Then,the dilution between 1:8 to 1:128were founded in 3rd week.The highest dilution with 1:256 appeared at 8th week,maintained before 11th week and then decreased gradually,which might provide a significant clinical implication for early diagnosis of circulating antigens.
2.Analysis of Constructing TCM Syndrome Complex Intervention Based on Text Mining and Interview Method
Feifei XUE ; Jiaxu CHEN ; Wenzhi HAO ; Lian GONG
World Science and Technology-Modernization of Traditional Chinese Medicine 2018;20(11):1954-1957
The occurrence, development and outcome of most diseases are a complex process. It is the advantage of traditional Chinese medicine to establish complex interventions that are consistent with the characteristics of disease development. The specific steps are as follows: quantitative research on literature research, establishment of interview framework; focus on interviews, specificization of interview outlines; semi-structured interviews, preliminary construction of complex intervention programs; evaluation of efficacy of complex interventions. The introduction of semi-structured interviews and other qualitative research methods into the modernization of traditional Chinese medicine, combined with quantitative methods such as text analysis and data mining, is also helpful in formulating the standard of diagnosis and treatment system with Chinese characteristics.
3.Comprehensive evaluation of the quality of Amomum tsao -ko based on entropy weight TOPSIS method
Jiaxu HAO ; Yuanzeng LI ; Xiao FAN ; Lichun ZHA ; Yunshu MA
China Pharmacy 2022;33(17):2087-2092
OBJECTIVE To evaluate the quality of Amomum tsao -ko from different origins and harvesting periods comprehensively. METHODS The contents of total volatile oil in A. tsao -ko were determined by volatile oil measurement method A stated in 2020 edition of Chinese Pharmacopoeia (part Ⅳ);the contents of total flavonoids and total polyphenols in A. tsao -ko were determined by aluminum nitrate-sodium nitrite colorimetry and folin-ciocalteu method. The contents of α-pinene,β-pinene, 1,8-cineole,α-terpineol,geraniol and trans-nerolidol in the volatile oil of A. tsao -ko were determined by gas chromatography ;the contents of protocatechuate and vanillic acid in A. tsao -ko were determined by ulta high performance liquid chromatography. The above 11 indicators were selected ,and entropy weight TOPSIS method was used to comprehensively evaluate the quality of 16 batches of A. tsao -ko. RESULTS The contents of total volatile oil ,total flavonoids ,total polyphenols ,α-pinene,β-pinene, 1,8-cineole,α-terpineol,geraniol,trans-nerolidol,protocatechuate and vanillic acid in 16 batches of A. tsao -ko were 15.833 3- 28.000 0 μL/g,29.100 5-78.199 6 mg/g,6.789 8-35.797 7 mg/g,0.088 7-0.401 3 mg/g,0.106 3-0.408 0 mg/g,3.709 6-8.533 1 mg/g,0.259 8-0.599 6 mg/g,0.314 8-1.324 1 mg/g,0.272 3-0.576 4 mg/g,9.301 2-19.818 5 μg/g,8.180 9-27.666 3 μg/g, respectively. Entropy weight TOPSIS results showed that the top three of relative closeness rankings were A. tsao -ko produced by Yunnan Baoshan in July ,Yunnan Honghe in October ,Yunnan Wenshan in September ;the last three of relative closeness rankings were A. tsao -ko produced by Yunnan Dehong in September ,Yunnan Dehong in November ,Yunnan Dehong in December. CONCLUSIONS A. tsao -ko produced by Yunnan Baoshan in July ,Yunnan Honghe in October and Yunnan Wenshan in September present better quality.
4.Efficacy and safety of endoscopic marking with SPOT for gastrointestinal lesions
Jiaxu WANG ; Shanshan WU ; Wenhai WANG ; Rongxue LI ; Yu ZHAO ; Weizhen ZHOU ; Yan WANG ; Xiujing SUN ; Peng LI ; Jianyu HAO ; Shutian ZHANG
Chinese Journal of Digestive Endoscopy 2022;39(9):701-706
Objective:To evaluate the efficacy and safety of SPOT (GI Supply, USA), a new carbon-based permanent marker approved by the Food and Drug Administration (FDA), in the endoscopic marking for gastrointestinal lesions.Methods:A total of 115 patients with gastrointestinal lesions who underwent endoscopic treatment or surgery in Beijing Friendship Hospital or Beijing Chao-Yang Hospital from April 2019 to November 2019 were enrolled in the study. SPOT was used to mark the lesions, and marking points were found during endoscopic treatment or surgery to calculate the effective marking rate by single-group target value method. Adverse events after marking were recorded, and the changes of blood routine test, liver and kidney functions before and after marking were compared.Results:The effective rate of endoscopic marking with SPOT was 99.13% (114/115). The longest marking time was 57 days. There was no puncture of intestinal wall or injection into abdominal cavity during the marking process. One patient developed mild fever after marking. The incidence of adverse events was 23.48% (27/115), which were all unrelated to the test equipment. There was no significant difference in blood routine tests or liver and kidney functions before and after marking ( P>0.05). Conclusion:SPOT produced by GI Supply can effectively mark gastrointestinal lesions without serious adverse events, which meets the requirements of clinical use.