0.05). Spike of [Ca 2+ ]i of astrocytes was induced by acute morphine administration, but the spike of [Ca 2+ ]i of astrocytes pretreated with morphine could be induced only by higher concentration of morphine. The [Ca 2+ ]i response of astrocytes to morphine could be blocked by naloxone but not by MK 801(NMDA receptor antagonist). Conclusion The response of [Ca 2+ ]i in morphine induced astrocytes may play an important role in morphine tolerance.

Sodium phenylacetate can induce differentiation of tumor cells and has been approved as a drug for the treatment of adults with cancer. In order to explore the immunological mechanism of its antitumor effect, the influence of sodium phenylacetate on HLA class I and II molecule expression in various human tumor cell lines, including breast adenocarcinoma (MCF-7.MUA-453), gastrocarcinoma(MKN-28,MKN-45), ovarian cancer(3AO) and cervical cancer(Hela), was studied with ELISA. The result showed that HLA class II molecule was absent from the surface of MCF-7 cells, but they could be induced after 7 days of continued treatment with sodium phenylacetate. Sodium phenylacetate was found to increase HLA class I molecule expression on the surface of MCF-7 cells, HLA class 1 and II molecule expression on the surface of MDA-453、 MKN-28、 MKN-45、 Hela and 3AO cells. The effect of sodium phenylacetate on HLA class I molecule expression in tumor cells is dose-and time-dependent.

Objective To explore the causes of accidental instability of SYSMEX XS-800i blood cell analyzer for WBC testing and their countermeasures.Methods Totally 10 pieces of specimen with high deviation were analyzed retrospectively, and the changes of DIFF-Y values of WBC were observed. Sysmex detergent was used to clean the pipelines including 4DL, 4DS and EPK pipes. Fluid path electromagnetic valve was checked and then repaired or replaced. Results It's proved that the results at the first time were not reliable, and there was significant differences between the results at the first and second times for DIFF-Y value, with P<0.01. The failures were eliminated after the above countermeasures were carried out.Conclusion WBC testing pipelines and electromagnetic valve of fluid path have to be cleaned periodically to eliminate the influence on DIFF-Y value of WBC and false increase of WBC counting.

Objective Percutaneous puncture of gasserian ganglion was guided by CT scan to improve the accuracy and reduce complications and to compare the efficacy of chemical mutilation with ethyl alcohol and adriamycin.Methods Ninety-two patients with primary trigeminal neuralgia (39 males, 53 females) aged 37-84 yrs were divided into two groups : A ethyl alcohol group ( n = 47) and B adriamycin group ( n = 45) . Secondary trigeminal neuralgia was excluded by preoperative CT scan. Percutaneous puncture of oval foramen was guided by CT and correct location of needle tip was confirmed by injection of contrast medium. 100% ethyl alcohol 0.5ml (group A) or 2% adriamycin 0.5ml was injected.Results The patients were followed up for 12 months after treatment. Neuralgia disappeared in 31 out of 47 patients in group A (alcohol group) and in 36 out of 45 patients in group B (adriomycin group) . Treatment failed in 13 patients in group A and 6 patients in group B, and was partly effective in the other patients. There were no severe side effects or complications. Conclusion Percutaneous puncture of gasserian ganglion guided by CT is effective and reduces complications. Adriamycin is more effective than ethyl alcohol for chemical mutilation.

Background and purpose:E-cadherin is a calcium-dependent cell adhesion molecule that mediates cell-cell adhesion and also modulates cell migration and tumor invasion.Many studies supported the role of E-cadherin as an invasion suppressor gene.It has been suggested that unlike E-cadherin,?-catenin might promote the invasion and metastasis of carcinoma.This study explored clinical pathological significance of E-cadherin and ?-catenin expressions in esophageal squamous cell carcinoma(ESCC).Methods:The PV immunohistochemical method was used to detect the expression of E-cadherin and ?-catenin in 62 cases of normal esophageal epithelium,31 cases of adjacent atypical hyperplasia epithelium and 62 cases of esophageal squamous cell carcinoma.Results:The positive rates of E-cadherin decreased by turns in the normal esophageal epithelium,adjacent atypical hyperplasia epithelium and esophageal squamous cell carcinoma(ESCC) specimens were 95.2%,71.0% and 40.3%,respectively.In normal esophageal epithelium,?-catenin showed higher intense expression at the membrane and lower intense expression in the cytoplasm.In contrast to the normal tissue,?-catenin was expressed in the cytoplasm of carcinoma in varied degrees,accompanied by less,or even negative expressions at the membrane.In some cases,?-catenin could be detected in the nucleus.Positive expression of ?-catenin(in cytoplasm) and negative expression of E-cadherin were related to the invasion,differentiation,and lymph node metastasis of ESCC(P

Objective To evaluate covered stent placement in the treatment of iliac artery rupture in the process of iliac artery angioplasty.Methods Clinical data of 9 patients' iliac artery rupture in the process of iliac artery angioplasty were retrospectively reviewed.Results Covered stent was successfully placed in all cases.The patency of involved iliac artery was identified in 8 cases by angiography and there was no contrast extravasation.Type Ⅰ endoleak occurred in one patient in the proximal end of the stent graft and iliac artery graft replacement was done by open surgery immediately.Postoperatively clinical symptoms were relieved in all these 9 patients.One case lost to follow up.8 patients were followed up for 14 to 45 months,average (22 ±9) months.Stenosis on the distal end of the covered stent (external iliac artery)was found in one patient who underwent external iliac artery balloon-expandable stent angioplasty.One patient with artificial vessel placement was symptoms free with patency of the involved artery as reviewed by ultrasonography on 18 month followed up.Conclusions When iliac artery rupture in the process of iliac artery angioplasty should develop,the first choice is immediately covered stent placement therapy,while open surgery remains as the last resort.

Objective To explore the joints and tendons characteristic features of high frequency ultrasound in asymptomatic hyperuricemia(AH) and to assess its value in AH diagnosis.Methods One hundred asymptomatic individuals with hyperuricemia (AH group) and 75 normouricemic subjects (control group) were examined by high frequency and color Doppler ultrasound.The joints,tendons and soft tissue of the knees,ankles and first metatarsal-phalangeal joints in two groups were examined.The correlations of serum urate(SU) level with double contour sign,bone erosion and articular cavity effusion were analysed.Results Hyperechoic deposition on the sulface of the articular hyaline cartilage (double contour sign) were found in the knees (35),ankles (17) and the first metatarsophalangeal joints (MTPJs,48) from the asymptomatic individuals with hyperuricemia,in contrast to none in the control group (P ＜0.05).Similar results were found in two groups of the bone erosion (30,25,20 vs 4,6,3,P ＜0.05),the effusion (28,36,41 vs 6,8,9,P ＜0.05) and the hyperechoic spots (19,18,22 vs 0,0,0,P ＜0.05).Patellar enthesopathy (4.5％ vs 0.6％,P ＜0.05) as well as achilles enthesopathy(8％ vs 0,P ＜0.05) and achilles tophi (6％ vs 0.6％,P ＜ 0.05) were more frequent in asymptomatic hyperuricemic than that of normouricemic individuals.No correlations between SU concentration and the presence of the double contour sign,the bone erosion and effusion were found (P ＞0.05).Conclusions There were some similar specific sonographic findings of gouty arthritis in the asymptomatic individuals with hyperuricemia.Also,these findings support high frequency ultrasound as a useful tool to detect anatomical damage in the joints,synovial tissue and tendons of asymptomatic individuals with hyperuricemia.

Objective To investigate the changes of glucocorticoid receptor (GR) gene expression in the hippocampus of rats following scald burn stress and the role of N methyl D aspartate (NMDA) receptor in this change. Methods Adult male Wistar rats inflicted with 30% TBSA full thickness scalding were applied as severe scalding stress model. GR mRNA levels in the hippocampus were detected with RT PCR. Results A significant decrease of GR mRNA levels was observed in the hippocampus 2 h after the scalding stress. The decrease could be inhibited when MK 801, an NMDA receptor antagonist, was administered prior to stress, and be augmented with the administration of NMDA, an NMDA receptor agonist, but not be affected by normal saline. Conclusion NMDA receptors are involved in the scalding stress induced down regulation of GR gene expression in the rat hippocampus.

BACKGROUND: How to solve the source of material substitute for repair of urethral dcfoct and improve the blood supply of new urethra has become a critical problem in the urethral repair and reconstruction.OBJECTIVE: To investigate the effects of endothelial progenitor cells (EPCs) on improving blood circulation in the new urethra following urethral defect repair.DESIGN,TIME AND SETTING: In vivo tissue engineering experiment,performed at the Laboratory of Deparanent of Surgery,First Affiliated Hospital of Zhengzhou University between January 2006 and February 2008.MATERIALS: Thirteen 3-5-month-old male Japanese rabbits were included for this study.Of them,one was used for preparation of bone marrow mononuclear cells,and the remaining twelve rabbits were divided into EPC repair group (n=8) and model group (n=4).METHODS: Under the aseptic condition,bone marrow was taken from the rabbit bilateral anterior superior lilac spine.Mononuclear cells isolated by Percoll method were induced in vitro using medium supplemented with vascular endothelial growth factors (VEGFs) and bovine basic fihroblast growth factors.When covering the whole bottom of culture flask,the mononuclear cells were digested with trypsin for passage.Animal models of urethral defect were developed in the two groups.One piece of aseptic fresh acellular human amnion (1 cm2) was sutured to each defected urethra using 0/6 DG suture for forming urethra.In the EPC repair group,1010/L passage 3 rabbit bone marrow mononuclear cell suspension was injected to two anastomotic stomas of the new urethra,0.1 mL for each stoma.The subcutaneous tissue was interruptedly sutured to the formed urethra using 0/6 DG suture.In addition,0.5 mL bone marrow mononuclear cell suspension was added to the region between each anastomotic stroma and newly repaired urethra.The same procedure was performed in the model group except that bone marrow mononuclear cell suspension was replaced by cell-free medium.At weeks 4 and 12 after surgery,paraffin sections of urethral tissue were made.MAIN OUTCOME MEASURES: Identification of cellular morphology; vascular regeneration of urethral tissue after urethral defect repair.RESULTS: After surgery,rabbit bone marrow mononuclear cells adhesively grew in vitro.Four days later,these cells exhibited rapid clone-like growth.Ten days later,they had typical slabstone-like change,presenting with strip-shaped and bundle-shaped growth.The phenotype of cultured cells gradually turned from CD34+/CD 133+/CD31+ to CD34+/CD 1337CD31+.At weeks 4 and 12 after surgery,the number of regenerate,d blood capillaries in the urethral tissue was significantly greater in the EPC repair group than in the model group (t=-9.034 to 5.985,P ＜ 0.01).CONCLUSION: Rabbit bone marrow mononuclear cells-differentiated EPCs can apparently improve local blood circulation in the urethral defect repair.

Objective To explore the effect of conservative treatment for Stanford B aortic intramural hematoma without calcified plaque and the midterm follow-ups.Methods Clinical data of 20 patients with Stanford B aortic intramural hematoma without calcified plaque which were confirmed by CTA of the whole aorta were retrospectively analyzed.There were 12 males and 8 females,with the age of 35～ 52 years.All of them received pharmacotherapy and psychotherapy.Results All the 20 cases were asymptomatic after conservative medical treatment when they discharged,and were followed up whit imaging tests.They all lived without symptoms during the follow-up period.The pleural fluid on the left side was disappeared in 11 cases after 3 months and the aortic intramural hematoma was absorbed in 13 cases after 6 months.After 1 year there were total 18 cases without aortic intramural hematoma,while The thickness and scope of the hematoma were reduced significantly in the other 2 cases.Conclusion It' s ease to find whether the patient with Stanford B aortic intramural hematoma got calcified plaque or not; the conservative treatment for Stanford B aortic intramural hematoma without calcified plaque has satisfactory clinical effect in midterm follow-ups.