Kidney transplantation (KT) is one primary treatment of end-stage renal disease (ESRD). As reported by different centers, 10-year survival rate of transplanted kidneys fluctuates from 75% to 80%. Capable of reducing all-cause mortality in renal failure, KT can significantly improve survival rate and quality-of-life of ESRD patients. The risk of post-transplant rejection is much higher in pre-sensitised recipients than in non-sensitised ones due to the pre-existing anti-human leukocyte antigen antibodies. However, with a rapid development of various desensitisation techniques, transplantation rate and postoperative human/kidney survival rate of recipients have been greatly enhanced. And presensitisation is no longer a contraindication to KT. This review focused upon the latest advances in desensitisation therapeutic agents for pre-sensitised KT.

Chronic kidney disease has been a global public health problem with a heavy disease burden.As a kidney replacement therapy, kidney transplantation(KT)has a higher long-term survival rate and quality-of-life as compared with dialysis.Antibody-mediated rejection(AMR)is a major complication after KT.Currently its core treatments are steroid hormones, plasma exchange and immunoglobulin.However, the effectiveness of new therapeutic agents such as anti-CD20 antibody and bortezomib has remained controversial.Despite these combination treatments, AMR is still a predominant cause of graft loss and it is imperative to seek novel effective treatments.Interleukin-6(IL-6)is a vital cytokine involved in the regulation of inflammation and the development, maturation and activation of T/B cells.Specifically targeting IL-6, ptolizumab and crazizumab are currently widely applied for managing AMR and blunting highly sensitized KT recipients.This review summarized the clinical applications of IL-6 for AMR.

Pancreatic cancer is a digestive system malignant tumor with extremely poor prognosis.With the rapid development of science and technology,although breakthrough results has been achieved in basic research of pancreatic cancer and some achievement in surgical techniques and adjuvant treatment approach,the survival of patients with pancreatic cancer has not been significantly improved.Among them,the serious disconnection between basic research and clinical practice has led to a slow improvement in the diagnosis and therapy.As a bridge between basic research and clinical work,translational medicine can promote progress of the early detection and precision treatment of pancreatic cancer,thus providing new hope for patients with pancreatic cancer.

Objective:To explore the effects of mindfulness-based stress reduction(MBSR)on pain relief,anxiety and depression and quality of life in naval personnel with chronic pain.Methods:A total of 72 naval person-nel with chronic pain were randomly divided into MBSR group and routine intervention group.The routine interven-tion group received routine care,while the MBSR group received MBSR in addition to routine care.The Short-Form McGill Pain Questionnaire(SF-MPQ),Self-Rating Anxiety Scale(SAS),Self-Rating Depression Scale(SDS),and 36-item Short Form Health Survey Scale(SF-36)were used at baseline and after 8 weeks of intervention.Results:The differences in the scores of 3 subjects of SF-MPQ,SAS,SDS and SF-36 in MBSR group at baseline and 8 weeks after intervention were higher than those in routine intervention group(Ps＜0.05).Conclusion:It suggests that mindfulnecs-based stress reduction could alleviate the degree of pain,anxiety and depression of patients with chronic pain in naval personnel and improve their quality of life.

OBJECTIVE: To explore the regulatory mechanism of human hepatocyte apoptosis induced by lysosomal membrane protein Sidt2 knockout. METHODS: The Sidt2 knockout (Sidt2^-/-) cell model was constructed in human hepatocyte HL7702 cells using Crispr-Cas9 technology.The protein levels of Sidt2 and key autophagy proteins LC3-II/I and P62 in the cell model were detected using Western blotting, and the formation of autophagosomes was observed with MDC staining.EdU incorporation assay and flow cytometry were performed to observe the effect of Sidt2 knockout on cell proliferation and apoptosis.The effect of chloroquine at the saturating concentration on autophagic flux, proliferation and apoptosis of Sidt2 knockout cells were observed. RESULTS: Sidt2^-/- HL7702 cells were successfully constructed.Sidt2 knockout significantly inhibited the proliferation and increased apoptosis of the cells, causing also increased protein expressions of LC3-II/I and P62(P < 0.05) and increased number of autophagosomes.Autophagy of the cells reached a saturated state following treatment with 50 μmol/L chloroquine, and at this concentration, chloroquine significantly increased the expressions of LC3B and P62 in Sidt2^-/- HL7702 cells. CONCLUSION Sidt2 gene knockout causes dysregulation of the autophagy pathway and induces apoptosis of HL7702 cells, and the latter effect is not mediated by inhibiting the autophagy-lysosomal pathway.
Humans ; Lysosome-Associated Membrane Glycoproteins/metabolism* ; Autophagy ; Apoptosis ; Hepatocytes ; Lysosomes/metabolism* ; Chloroquine/pharmacology* ; Nucleotide Transport Proteins/metabolism*