[Objective]To study the repair of articular cartilage defects with combined bone morphogenetic protein(BMP)/basic fibroblast growth factor(bFGF)biomaterial in order to supply experimental basis for repairing cartilage defects biologically.[Method]Twenty-four 14-week-old rabbits were randomly divided into four groups,BMP/bFGF biomaterial gel(group A),BMP biomaterial gel(group B),bFGF biomaterial gel(group C)and simple fibrin glue treated group(group D).A couple of knees of each rabbit experienced 5 mm-diameter-full cartilage resection and drilling throuth of bone marrow.Gross appearance,histologicol section and electron microscope were axamined at 8,12,24 weeks after operation.[Result]In group A the cartilage defects were smoothly repaired by white translucent hard tissue at 8 and 12 weeks,and defect boundary was hard to be indentified with normal cartilage at 24 weks.No smooth repairing was observered in B,C and D groups.Group A got a better histological score than B,C and group D(P0.05).[Conclusion]Combined BMP/bFGF biomaterial is good for repair of articular cartilage defects and has better result than using BMP or bFGF alone.These results may provide a therapy for articular cartilage defects.

Objective To systematically evaluate the performance of the Sysmex CS2000i Automatic Blood Coagulation Analysis System .Methods According to the standards of the Clinical and Laboratory Standards Institute (CLSI) ,the precision ,accuracy ,lin‐earity ,biological reference interval and carry‐over rates of the Sysmex CS2000i Automatic Blood Coagulation Analysis System were detected ,meanwhile the detections of 5 clinical specimens were compared between this system and the Sysmex CA 1500 Automatic Blood Coagulation Analysis System .Results The intra‐assay precision coefficient of variation (CV) ,inter‐day precisions CV carry‐over rate ,accuracy and linearity of plasma prothrombin time ,activated partial thromboplastin time ,fibrinogen and D‐dimmer detec‐ted by the Sysmex CS2000i Automatic Blood Coagulation Analysis System were all consistent with the quality target requirements of our laboratory .Conclusion The Sysmex CS2000i Automatic Blood Coagulation Analysis System has better performance in vari‐ous aspects ,the detection results could be used for the clinical diagnosis and treatment of related diseases .

Objective To compare the efficacies of da Vinci robotic and traditional laparoscopic resection for colorectal cancer.Methods Databases including Pubmed,Cochrane Library,EMBASE,Medline,Wanfang,CNKI,VIP and FMJS were searched,and literatures published before February 2012 were retrieved.Randomized controlled trials (RCTs) and non-randomized controlled trials (NRCTs) focusing the comparison between da Vinci robotic and laparoscopic resection for colorectal cancer were collected.All the literatures retrieved were screened according to preset standards,and the patients were divided into robotic group and laparoscopic group.A meta analysis on the effectiveness of robotic and laparoscopic surgery for colorectal cancer was carried out using the RevMan 5.1 software.Categorical variables were presented by odds ratio (OR) and 95％ confidence interval (95 ％ CI),continuous variables were presented by weighted mean difference (WMD) and 95 ％ CI.Results One RCT and 10 NRCTs including 974 patients with colorectal cancer were screened out and there were 426 patients in the robotic group and 548 patients in the laparoscopic group.Compared with conventional laparoscopic surgery,longer operation time,lower rate of conversion to laparotomy and shorter anal exsufflation time were observed in the robotic group (WMD =25.61,OR =0.32,WMD =-0.21,P ＜ 0.05).There were no significant differences in the operative blood loss,number of lymph node dissected,distal resection margin,morbidity and duration of hospital stay between the robotic group and the laparoscopic group (WMD =-23.14,-0.31,0.14,OR =1.06,WMD =-0.43,P ＞ 0.05).Conclusion The efficacies of da Vinci robotic and laparoscopic surgery for the treatment of colorectal cancer are comparable,while da Vinci robotic surgery has the features of lower rate of conversion to laparotomy and shorter anal exsufflation time.

BACKGROUND:The main therapy of severe aplastic anemia in children is immunosuppressive therapy or stem celltransplantation, but the latter one is restricted due to few donor sources. Haploidentical hematopoietic stem celltransplantation is commonly used in leukemia, but it is stil rarely reported in the treatment of aplastic anemia. OBJECTIVE:To investigate the effect of haploidentical hematopoietic stem celltransplantation combined with placenta-derived mesenchymal stem celltransplantation for children with severe aplastic anemia. METHODS:A 7-year-old girl who had been confirmed as having severe aplastic anemia for 1.5 years received a cotransplantation of haploidentical hematopoietic stem cells combined with placenta-derived mesenchymal stem cells on July 9th , 2012. The donor was her mother. The preconditioning regimen consisted of fludarabine, cyclophosphamide, and anti-thymocyte globulin. RESULTS AND CONCLUSION:Time of neutrophil recovery (>0.5×10 9/L) was+9 days, and hematopoietic reconstruction was complete at+12 days. The short tandem repeat analysis showed 100%donor’s genotype at+100 days. Immunosuppressive drugs were stopped at+8 months, and no acute or chronic graft-versus-host disease occurred. With a fol ow-up of 18 months, she was in the disease-free survival period. Our findings suggest that the cotransplantation of al ogeneic haploidentical hematopoietic stem cells and placenta-derived mesenchymal stem cells is a new effective approach for children with severe aplastic anemia, which is worth exploring in the future.

Objective To explore the preoperative localization diagnosis and surgical techniques of intractable occipital lobe epilepsy.Methods Retrospectively studied 37 patients diagnosed as occipital lobe epilepsy and underwent focal occipital resections for epilepsy.The semiology,scalp electroencephalography,MRI,fluorodeoxyglucose-positron emission tomography(FDG-PET),and intracranial EEG monitoring were used to localize the epileptogenic zones.The long-term seizure outcomes were assessed according to the Engel classification scheme.Results Visual symptoms were present in 25 patients preoperatively in this series.MRI displayed occipital lobe lesions in 15 patients,and FDG-PET revealed hypometabolism in or adjacent to epileptogenic zones.And 30 patients' epileptogenic zones and functional areas were defined by intracranial EEG monitoring.Visual field deficits were present in 35.3% of patients preoperatively,and 61% had new or aggravated visual field deficits after surgery.After a mean follow-up of 41 months,81.1% of the patients were seizure free or rarely had seizures.Conclusion The curative effect of the surgery on the medically intractable occipital lobe epilepsy is good.Intracranial EEG monitoring with electrodes extensively covering the occipital lobe and adjacent areas can be useful to demarcate the epileptogenic zones and the visural cortex,and it may prevent aggravation of the visual field deficits as much as possible.

Objective: To explore the effectiveness of dynamic hip screw (DHS) and intralesional curettage via Watson-Jones approach in treatment of benign bone lesions of the proximal femur.

Objective To investigate the different memory outcomes in temporal lobe epilepsy patients underwent different surgical approaches.Methods Two hundred forty-eight patients with temporal lobe epilepsy and hippocampal scle-rosis underwent standard anterior temporal lobectomy ( ATL, n=83 ) or selective amygdalohippocampectomy ( SAH, n=165) from 2009 to 2013.All the patients underwent clinical memory function assessment before surgery, 3 months and 2 years after surgery respectively.Results The memory quotient ( MQ) of patients who underwent brain surgery in the domi-nant hemisphere significantly decreased 3 months after surgery (74.5 ±16.2, 75.6 ±19.5) compared to presurgery MQ (82.9 ±15.8, 83.2 ±21.2) in both ATL and SAH groups (P<0.05).Although MQ was slightly recovered at 2 years af-ter surgery, MQ (75.1 ±14.1, 76.1 ±17.6) was still significantly lower compared with presurgery MQ (P<0.05).A-mong this, both the decrease extent of the MQ 3 months after surgery and 2 years after surgery were smaller in the SAH group than in the ATL group (7.6 vs.8.4;7.1 vs.7.8).The MQ of patients who underwent brain surgery in the non-dominant hemisphere (either ATL or SAH ) increased slightly 3 months after surgery (87.2 ±15.1, 88.1 ±16.9) com-pared to presurgery MQ (85.5 ±13.5, 85.3 ±19.7) although the difference was not statistically significant.The MQ of these two groups improved significantly 2 years after surgery (92.8 ±12.7, 93.7 ±17.1)(P<0.05).The improvement extent of the MQ was larger in the SAH group than in the ATL group (8.4 vs.7.3).Conclusions SAH may be better than ATL in the maintenance of memory function in patients with temporal lobe epilepsy and hippocampal sclerosis.

ObjectiveTo investigate the relevant risk factors for early recurrence after microwave ablation for primary hepatocellular carcinoma (HCC), and to provide a reference for predicting and preventing tumor recurrence. MethodsA retrospective analysis was conducted on the clinical data of 80 patients who had undergone microwave ablation for primary HCC at the Beijing You′an Hospital, Capital Medical University, Beijing, China (2010-2012). Factors possibly influencing early recurrence were selected for univariate analysis using logistic model. Risk factors for early recurrence of HCC after ablation were screened out for identifying the high-risk population and further guiding treatments against recurrence. ResultsThere were 30 cases of recurrence in 6 months after ablation, accounting for a recurrence rate of 375%. Univariate analysis showed that no significant differences occurred between the recurrence and non-recurrence groups in age, gender, tumor location and size, family history of HCC, drinking history, HCC resection history, preoperative alpha-fetoprotein level, liver function Child-Pugh grade, MELD score, or HCC-related causes (P＞0.05). In contrast, the number of tumors (≥2) significantly differed between the two groups (P=0.008). ConclusionThe number of tumors is an independent risk factor for early recurrence among patients after microwave ablation for HCC. High-risk population can be predicted according to the risk factors for early recurrence after microwave ablation for HCC. Targeted preventive measures should be taken for early detection and timely treatment of tumor recurrence.

Objective:To screen the potential biomarkers and drugs in acute myeloid leukemia (AML) to improve the cure rate of leukemia.Methods:The GeneChip GSE90062 dataset (including 3 leukemia stem cell samples and 3 normal bone marrow hematopoietic stem cell samples) and GSE17054 dataset (including 9 leukemia stem cell samples and 4 normal bone marrow hematopoietic stem cell samples), which included AML patients and healthy donors, were downloaded from the Gene Expression Omnibus (GEO) database. Common differentially expressed gene (DEG) between bone marrow of AML patients and healthy donors in both GEO database datasets was analyzed by GEO2R software. Gene Ontology (GO) biofunction enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of common DEG were performed using DAVID online software. The protein-protein interaction (PPI) networks were constructed using the STRING database, and key genes in the top 10 of the correlation intensities were screened with the help of CytoHubba plug-in. Gene Expression Profiling Interaction Analysis (GEPIA) database was applied to reconfirm the key genes from the 10 key genes; Kaplan-Meier survival curves were used to assess the prognosis of AML patients with different levels of the 10 key genes, and comparisons were made using the log-rank test; the Drug-Gene Interaction Database (DGIdb) was used to screen for the key gene-related drugs.Results:A total of 75 up-regulated common DEG and 61 down-regulated common DEG were identified. In GO enrichment analysis, common DEG was mainly associated with cell apoptosis and cell migration. In KEGG pathway analysis, they were mainly related to apoptosis and hematopoietic pathway. The top 10 key genes with strong correlations screened by the PPI networks were ITGA4, ITGAL, HNRNPA3, CDC42, PRF1, SRSF3, HNRNPD, GTPBP4, CXCR4, and RPL35A, of which 6 genes (ITGA4, ITGAL, HNRNPA3, GTPBP4, CXCR4, and RPL35A) were reconfirmed by the GEPIA2 database. Kaplan-Meier survival curve analysis showed a statistically significant difference in poor overall survival of AML patients with high expression of ITGAL compared to patients with low expression of ITGAL ( P = 0.010); the difference in overall survival between patients with high and low expression of the remaining 9 key genes was not statistically significant (all P > 0.05). Four potential drugs which may be related to ITGAL were screened from DGIdb, they were efalizumab, odulimomab, lifitegrast, and rovelizumab. Conclusions:ITGAL is overexpressed in the bone marrow of AML patients compared to healthy individuals and it is an unfavorable prognostic factor for AML patients. The screened potential drugs targeting ITGAL (efalizumab, odulimomab, lifitegrast, and rovelizumab) provides new ideas for the treatment of AML.

Objective:To discuss the problems that exist in the operation mechanism of the third -party media-tion mode of medical disputes in China and put forward suggestions for improvement , through comparative study of the third-party mediation mode of domestic and foreign medical disputes .Results:People's mediation mechanism of medical disputes in our country still hadsome problems .For example , mediation talents were insufficient;propa-ganda was inadequate;mediation results were difficult to implement;mediation support funds couldn ' t be guaran-teed, and so on.Conclusion:It is recommended to improve the remuneration of employees and standardize the staff selection system;increase propaganda and raise public awareness;improve the judicial confirmation system and en-hance the guarantee of agreement;save regulation cost and increase multi -financing channels .