Objective To explore the effective therapies of no n-small cell lung cancer(NSC LC) of advanced stage.Method.Two hundred twenty nine patient s with NSCLC at advanced stage were treated by thr ee methods including comprehensive therapy,chemotherapy and radiotherapy.Cur ativ e effects of various therapy methods were analyzed.Result The to tal effective rates o f the patients who received comprehensive therapy were obviously higher than che motherapy and radiotherapy alone groups,they were 68.96%,49.17%,50.00%(P ＜0.01) respectively.Conclusion The preference method was c omprehensive therapy.A clos e relationship was found among the total efficacy and pathological classificat ion and clinical staging.

Objective To analysis the levels of serum homocysteine (Hcy)and erythropoietin (EPO)levels in patients with ischemic stroke and their relationship with carotid atherosclerosis.Methods The patients with ischemic stroke admitted be-tween January 1,2013 and July 31,2016 were selected as the subjects.Sera test of Hcy,EPO level,and then through the Doppler probe carotid artery method to check the degree of carotid atherosclerosis in all patients,and the use of quantitative records,according to set diagnostic criteria for classification,the use of grading results with the same type of Hcy,EPO lev-els of data analysis to determine the relationship between the two.Results Doppler exploration in 142 patients showed nor-mal carotid intima-media thickness in 34 patients,carotid intima-media thickening in 56 patients,and carotid plaques in 52 pa-tients.After analysis of variance (F=59.64,78.29,P<0.05),and the difference between the two groups was statistically significant (P<0.05),and the difference between the two groups was statistically significant.The data between any two groups were also statistically significant differences.108 patients with different levels of carotid atherosclerosis were graded into 47 of grade 1,34 of grade 2,and 27 of grade 3,with the same type in the plasma of different grades (F=78.39,48.61, P<0.05).After the qtest,there were no significant differences in the levels of Hcy and EPO.Data were also statistically dif-ferent between groups.Conclusion Serum homocysteine (Hcy)and erythropoietin (EPO)were associated with ischemic stroke and were associated with carotid atherosclerosis.

Objective To study the relationship between serum MMP-9,Gal-3,TGF-β1 and lung function in children with bronchial asthma.Methods The serum levels of matrix metalloproteinase-9 (MMP-9),serum galectin-3 (MMP-9),serum metalloproteinase-3 (MMP 9) and serum albumin were measured in 218 pediatric children between March 1,2012 and August 31,2016 in Shenzhen Longhua New District People's Hospital pediatric outpatient clinic.FEV1％,FEV1/FVC and FEF50 were measured and compared respectively with the index data of the control group.The lung function of the children was significantly higher than that of the control group (P＜0.05).Mild group and severe group children above.Results Serum levels measured in 218 children (F=35.34,46.51,56.73,all P＜0.05).The data of any two groups also showed statistical difference after q test,and the difference was statistically significant (FEV1 ％),1-second forced expiratory volume/forced vital capacity percentage (FEV1/FVC),forced expiratory 50％ (FEF50) of the first-second forced expiratory volume/forced expiratory volume of 1 second (F=78.13,52.69,26.24,all P＜0.05).After the q test,the data of any two groups were analyzed by ANOVA.Between the data were also statistically significant differences.Conclusion MM-9,Gal-3 and TGFβ1 in bronchoalveolar lavage and the degree of pulmonary function decline have close ties.

Objective To explore the effective therapies of non-small cell lung cancer(NSCLC) of advanced stage.Method.Two hundred twenty nine patients with NSCLC at advanced stage were treated by three methods including comprehensive therapy,chemotherapy and radiotherapy.Curative effects of various therapy methods were analyzed.Result The total effective rates of the patients who received comprehensive therapy were obviously higher than chemotherapy and radiotherapy alone groups,they were 68 96%,49 17%,50 00%(P

Objective To review the treatment results of intracytoplasmic injection(ICSI) of epididymal or testicular sperm obtained from 38 obstructive azoospermic patients.Methods Sperm was retrieved by percutaneous epididymal sperm aspiration(PESA) or testicular sperm extraction(TESE).Intracytoplasmic injection was performed.The rates of fertilization and clinical pregnancy were evaluated.Control group was set up in which intracytoplasmic injection was performed using sperm of ejaculation.Results Forty-one treatment cycles were performed in the 38 obstructive azoospermc patients.The rates of fertilization and clinical pregnancy were 73.3% and 53.6%.Thirty-three treatment cycles were done in the 31 ejaculatory ones.The rates of fertilization and clinical pregnancy were 75.1% and 48.4%.No significant difference was seen between the two groups.In the obstructive azoospermia group,22 clinical pregnancies were achieved including 13 live deliveries and 3 ongoing pregnancies and 6 miscarriages.In the ejaculatory group,16 clinical pregnancies were achieved including 10 live deliveries and 5 ongoing pregnancies and 1 miscarriages.Conclusions ICSI with PESA or TESE is an effective method for treatment of obstructive azoospermic patients.

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Objective The aim of this study was to investigate the relationship between serum C-reactive protein (CRP) and muscle strength, physical performance in the elderly. Methods A cross-sectional study was conducted in Hangu district of Tianjin from April to June in 2013. Totally 1 131 subjects aged 60 years or older completed the survey. Every subject filled out a standard questionnaire and underwent grip strength test, timed up and go test (TUGT). Serum CRP concentrations were determined using chemiluminescent immunoassay kits. Multivariate analysis was performed to determine the relationship between CRP and muscle strength, physical performance. Results After adjustment for potential confounding factors including age, BMI, smoking status, drinking status, hypertension, diabetes, cardiovascular disease, anemia, cancer and physical activity, the multiple linear regression analysis suggested that CRP levels were inversely related to handgrip strength (β'=-0.11, P<0.01), and the logistic regression analysis showed that compared with subjects in the lowest quintile, the odds ratios and 95%confidence interval of higher quintile of CRP were 1.09 (0.50, 2.39) and 2.70 (1.34, 5.66) (Ptrend<0.05) in males. However, the relationship between CRP and muscle strength, physical performance was not statistically significant in females. Conclusions In males, higher serum CRP levels were independently correlated with lower muscle strength and poor physical performance in older adults. These results suggested that inflammation-signaling pathways may play an important role in the progression of sarcopenia.

Aim: To improve the synthesis for 2, 5-anhydro-3,4, 6-tri-O-benzyl-D-glucitol. Methods: Dehydration of D-mannitol, followed by a reaction sequence of selective protection, benzylation, deprotection, tritylation, benzy-lation and removal of the trityl group, afforded the title compound. Results: Based on the improvement of the reported synthetic route, 2,5-anhydro-3,4,6-tri-O-benzyl-D-glucitol was synthesized in seven steps from D-mannitol with a total yield of 8. 3% . Conclusion: This new method features mild reaction conditions and facile work-up.

Dig-DNA probes of hemorrhagic fever with renal syndrome vinus M S gene fragment were prepared by PCR and random primer labeling, The probes had speciality to HFRSV-RNA and could detect 10pg RNA by RNA-DNA dot-blot hybridization. The results of hybridization showed that positive reactions were found in 5, 10 chigger mites groups from antigenic positive rats, 10 mites group from antigenic negative rats, 10, 50 free chigger mites, and hung of antigenic positive mice 100mg, 500mg. But negative reation in 5 mites group from antigenic negative mice.

Objective To summarize the experience of intraoperative rapid parathyroid hormone (PTH) detection by immunochromatography in thyroid surgery.Methods Patients undergoing thyroid surgery in Shanghai Tenth People's Hospital from Nov.2016 to Jul.2017 were collected and the clinical data including level of blood calcium,serum phosphorus and PTH,and pathological findings were analyzed.Results In this study,im munochromatographic assay was used in 45 cases (experimental group) and nano carbon method was used in 47 cases (control group).In benign cases,levels of blood calcium and PTH in experimental group were higher than those in the control group ((2.07±0.09) vs (2.04±0.06) and (3.41±1.82) vs (3.34±1.84),P＞0.05).In malignant cases,blood calcium level in experimental group was higher than that in the control group ((2.02±0.13) vs (1.99±0.38),P＞0.05) and PTH level was significantly higher than that in the control group ((2.22±1.27) vs (1.44±1.44),P＜0.05).Serum phosphorus level in experimental group group was much lower than that in the control group ((1.02±0.17) vs (1.14±0.23),P＜0.05).Conclusions Using intraoperative rapid PTH detection by immunochromatography can identify parathyroid and avoid accidental injury of parathyroid gland,which can reduce the postoperative incidence of hypocalcemia.