Objective To investigate the red blood cell in urine after kidney puncture and know the best detection method.Methods Collected three urines of 31 patients after kidney puncture and detected the red blood cell by UF-100 and the hemoglobin by urine dipstick test.At the same time,detect the conductivity of the urine by UF-100.Results Both the UF-100 and urine dipstick test could reflect the decrease of red blood cell after kidney puncture.But both of them had some limits.Sometimes the results of the two method were not relative(κ=0.148).The agreement of two methods was 0.538.Neither of them could reflect the bleeding of the kidney accuracy.Conclusion By the best,we should use both the UF-100 and the urine dipstick test to detect the red blood cell in urine after kidney puncture,at the same time we should consider the conductivity of the UF-100.

Objective To assess clinical safety and effect of intracoronary transplantation of autologous bone marrow cells in patients with acute myocardial infarction(AMI).Methods Eighty four AMI patients who had received emergency thromblysis or primary PTCA were enrolled in this study.Elective PCI was undergone in these patients 10-14 days after infarction.During the procedure,50 patients received introcoronary transplantation of autologous bone marrow derived mononuclear cells and the other 34 patients received normal saline as control.All patients achieved TIMI Ⅲ flow after PCI.Dobutamin stress echocardiography,SPECT and F-18-Fluorodeoxyglucose-PET were performed 1 day before and 6 months after the transplantation.All patients finished a 2-year follow up and stress echo examination.Twenty nine patients from the transplantation group and 22 patients from the control group accepted 6-month SPECT reassessment.Results No major adverse events were recorded in all patients who received autologous bone marrow cells transplantation during follow up.Less nitroglycerin usage and increased excercise were observed in the transplantation group.Stress echocardiography showed improvement in LVEF(27.00%?0.89% pre-operation,36.80%?0.58% after 6 months and 40.94%?0.58% after 2 years,P

Objective To study the clinical characteristics of gastrointestinal (GI) hemorrhage in SARS and factors affecting its occurrence. Methods Retrospective study on clinical data of SARS cases complicated with gastrointestinal hemorrhage admitted to our hospital. Results Of 220 SARS cases gastrointestinal hemorrhage occurred in 20 patients (9% ) , with 13 males, 7 females, age ranged 19 -83 years (50?20) , onset at the 5th to 35th day of illness (22?9) , hematemesis/melena in 5 cases, positive occult blood test in 15. After treated with famotidine, omeprazole, reptilase and thrombin bleeding stopped in 16 cases within 7 to 10 days. Four cases died of respiratory failure. Rate of hemorrhage was lower in patients receiving prophylactic therapy with H2 receptor blocker famotidine than those without prophylactic therapy (P

Objective To evaluate the results of teaching in both Chinese and English with 7-year-program clinical medicine majors and analyze the influencing factors.Methods Data from a questionnaire survey of 25 students exposed to bilingual teaching were analyzed.Results The students generally approved of the model of bilingual teaching,which was contributory to improving their proficiency in specialized English.However,uneven English levels on the part of teachers and students and imperfect materials and methods as well as discontinuity affected the overall results of bilingual teaching and to some extent the students' mastery of specialized knowledge.Conclusion Bilingual teaching with 7-year-program clinical medicine majors is a must,and yet persistent efforts need to be made in enhancing the teachers' English level,compiling proper textbooks and improving teaching methods so as to genuinely better the results of bilingual teaching.

AIM: To explore the effect and mechanism of preoperative transarterial chemoembolization on nephroblastoma.METHODS: Comparative analysis of clinical and pathological features in 39 children with Wilms’ tumor was conducted. TUNEL assay was used to detect the apoptosis of tumor in two groups with or without preoperative interventional treatment. The expressions of P53, Bcl-2 and Bax proteins were detected by immunochemical methods. The patients were followed-up for more than 2 years.RESULTS: The extent of neoplastic cell necrosis and degeneration, interstital fiber tissue hyperplasia of tumor and the number of infiltrating lymphocytes were observed, which were higher in interventional group than those in simple excision group (P

OBJECTIVE: To figure out the etiological factors and overall mortality of the patients with acute intestinal obstruction, and to explore the rational period of conservative therapy before operation. METHODS: Medical records of all the patients with acute intestinal obstruction admitted to West China Hospital from 1995 to 2002 were retrospectively reviewed. The etiology of the obstruction was categorized, and the correlation of mortality and time interval between conservative therapy and operation was analyzed. RESULTS: There were 705 patients with acute intestinal obstruction included. There were 71.1% of the obstruction lesions located on the small bowel, and 82.6% of the patients experienced simple obstruction. The most frequent cause was adhesions (62.0%), and next was neoplasms (23.7%). There were 57.6% of the patients underwent the surgical treatment. The overall mortality rate was 1.6%, and the mortality rates in conservative therapy and surgical intervention groups were 1.3% and 1.7% respectively. The intestinal necrosis rate was increased gradually with the prolongation of time interval between conservative therapy and operation, and the death might occur 24 hours after strangulation. CONCLUSION: The epidemiological transition to adhesive obstruction still exists in China, and it is similar to that in Western countries. In our experience, near half of the patients with simple obstruction may achieve palliation by conservative therapy. Surgical intervention is indicated for the patients with prolonged and non-palliated simple obstruction, or strangulation disease within the first 24 hours.

AIM:To evaluate the safety and feasibility of using vascular endothelial growth factor (VEGF) in the establishment of Walker-256 transplanted liver cancer model .METHODS: SD rats ( n =45) were divided into 3 groups:via the caudal vein, the rats in normal saline (NS) group were injected with 0.9%sodium chloride (0.1 mL/d), the rats in 20 mg/L VEGF group were injected with 20 mg/L VEGF (0.1 mL/d), and the rats in 40 mg/L VEGF group were injected with 40 mg/L VEGF (0.1 mL/d).All the injection began 1 week before transplantation of liver cancer , and stopped on the day the cancer model was established .Prepared tumor tissue was transplanted into the subcapsular space of the liver.Three days, 1 week and 2 weeks after the transplantation, magnetic resonance imaging (MRI) was performed for analyzing the tumor growth and the characteristics .The overall survival of the rats was also recorded .RESULTS:Success-ful establishment of Walker-256 transplanted liver cancer model was achieved .Among 45 rats, 1 rat died 1 d after implan-ting the tumor both in NS group and 20 mg/L VEGF group, while 3 rats died in 40 mg/L VEGF group 1 week after building the model, mainly because of the progression of tumors .Three days after modeling,the numbers of the rats in which the tumor was positively detected by MRI in 3 groups were 0, 7 and 10, respectively;1 week after modeling, those were 3, 13 and 13, respectively;2 weeks after modeling,those were 12, 13 and 10, respectively.Between NS group and 20 mg/L VEGF group, the statistical significance existed in the number of the rats in which the tumor was positively detected by MRI after 3 d of implanting, so did the NS group and 40 mg/L VEGF group.No statistical significance in the overall survival time between NS group and 20 mg/L VEGF group (P>0.05) was observed, but the significance existed between 40 mg/L VEGF group and NS group (P<0.01).CfONCLUSION:The application of VEGF at dose of 20 mg/L and 0.1 mL/d shortens the time to establish the transplanted liver cancer model without influence on the overall survival , which is a safe, feasible and efficient way, and is more suitable for anti-VEGF drug investigation.

Objective To investigate the role of alprostadil on hepatic perfusion after transarterial chemoembolization(TACE) for hepatocellular carcinoma. Methods Sixty-four consecutive patients with HCC were randomized to either treatment with PGE_1 after TACE (treatment group, 32 cases) or no additional treatment after TACE (control group, 32 cases). In PGE_1 group, Lipo-PGE_1 was administered intravenously once a day for total of seven days, once after completion of TACE. The dosage of Lipo-PGE_1 was 0.4μg/kg and rote 0.05 μg·kg~(-1)·min~(-1). In control group, regular TACE was used. All patients underwent hepatic CT perfusion within 1 week before TACE and 4 weeks after TACE. The parameters of hepatic perfusion, including hepatic arterial perfusion value (HAP), portal vein perfusion value (PVP), total liver perfusion value (TLP) , and hepatic arterial perfusion index (HPI) were measured and compared. Chi-Square test was used for comparison of CT perfusion parameters in different stage, and t test was used for comparison of each CT porfusion parameter between two groups. Results In control group, HAP of pre-TACE, 4 weeks after first TACE, and 4 weeks after second TACE was (0.18±0.08), (0.22±0.09), (0.32±0.10) ml·min~(-1)·ml~(-1), respectively. Likewise, PVP was (1.11±0.31)、(0.82±0.27)、(0.59±0.25) ml·min~(-1)·ml~(-1), respectively, and TLP was (1.29±0.33), (1.04±0.28), (0.91±0.24) ml·min~(-1)·ml~(-1), respectively, and HPI was (14.31±6.36)%, (21.37±9.07)%, (36.67±13.42)%, respectively. The perfusion parameters at different stages of TACE were statistically different (F=19.71,27.47,14.75,41.41, P<0.05). In PGE1 group, HAP before TACE, after first TACE, and after second TACE was (0.17±0.08), (0.20±0.08), (0.26±0.08) ml·min~(-1)·mi~(-1) respectively, and PVP was (1.09±0.36), (1.03±0.40), (0.91±0.41) ml·min~(-1)·ml~(-1), respectively, and TLP was (1.26±0.38), (1.23±0.40), (1.17±0.44) ml·min~(-1)·ml~(-1) respectively, and HPI was (14.04±6.71)%, (17.26±7.86)%, (23.93±8.96)%, respectively. The difference of HAP and HPI at different stage of TACE was significant (F = 10.78, 13.05, P < 0.05), but there was no significant difference both PVP and TLP (F = 1.73,0.39, P > 0.05). The difference of PVP and TLP between the control and PGE1 group was significant after first TACE(t = -2.37, -2.14, P <0.05)and second TACE (t = 2.55, - 4.49, P < 0.05) In addition, after the second TACE, the HAP and HPI were also significantly different (t = - 3.41,5.09, P < 0.05). Conclusions PVP and TLP decrease while HAP and HPI increase after TACE. Lipo-PGE1 improves hepatic peffusion after TACE, exerting its greatest effect by increasing portal vein perfusion. Consequently, treatment with Lipo-PGE1 appears to increase liver tissue perfusion and thereby alleviate injury induced by TACE.

alterations on liver hemodynamics on IR injury following administration of medication.

s and no restenosis was found in 3 patients according to cerebral angiography. Preliminary results show that PTAS in the management of the vertebrobasilar arterial stenosis is a safe and effective method.