ObjectiveTo investigate the protective effect of the extract of Liuwei Dihuangwan （LW） on mitochondrial damage in the Alzheimer's disease （AD） model of Caenorhabditis elegans （C. elegans）. MethodC. elegans transfected with human β-amyloid protein （Aβ） _1-42 gene was used as an AD model. The rats were divided into blank group， model group， metformin group （50 mmol·L^-1）， and low， medium， and high dose （1.04， 2.08， 4.16 g·kg^-1） LW groups. Behavioral methods were used to observe the sensitivity of 5-hydroxytryptamine （5-HT） in nematodes. Western blot was used to detect the expression of Aβ in nematodes. Total ATP content in nematodes was detected by the adenine nucleoside triphosphate （ATP） kit， and mitochondrial membrane potential was detected by the JC-1 method. In addition， the mRNA expression of Aβ expression gene （Amy-1）， superoxide dismutase-1 （SOD-1）， mitochondrial transcription factor A homologous gene-5 （HMG-5）， mitochondrial power-associated protein 1 （DRP1）， and mitochondrial mitoprotein 1 （FIS1） was detected by real-time fluorescence quantitative polymerase chain reaction （RT-PCR）. ResultThe extract of LW could reduce the hypersensitivity of the AD model of nematodes to exogenous 5-HT （P<0.05） and delay the AD-like pathological characteristics of hypersensitivity to exogenous 5-HT caused by toxicity from overexpression of Aβ in neurons of the AD model of nematodes. Compared with the blank group， in the model group， the mRNA expression of Aβ protein and Amy-1 increased （P<0.01）， and the mRNA expression of SOD-1 and HMG-5 decreased （P<0.01）. The mRNA expression of DRP1 and FIS1 increased （P<0.01）， and the level of mitochondrial membrane potential decreased （P<0.05）. The content of ATP decreased （P<0.01）. Compared with the model group， in the positive medicine group and medium and high dose LW groups， the mRNA expression of Aβ protein and Amy-1 decreased （P<0.05，P<0.01）， and the mRNA expression of SOD-1 and HMG-5 increased （P<0.01）. The mRNA expression of DRP1 decreased （P<0.05，P<0.01）， and that of FIS1 decreased （P<0.01）. The level of mitochondrial membrane potential increased （P<0.01）， and the content of ATP increased （P<0.05，P<0.01）. ConclusionThe extract of LW may enhance the antioxidant ability of mitochondria， protect mitochondrial DNA， reduce the fragmentation of mitochondrial division， repair the damaged mitochondria， adjust the mitochondrial membrane potential， restore the level of neuronal ATP， and reduce the neuronal damage caused by Aβ deposition.

Objective:To investigate the clinical efficacy of 3D printed metal augment or tibial prosthesis for reconstruction of large bone defects in total knee arthroplasty (TKA) and knee revision surgery.Methods:A total of 7 patients (7 knees) with TKA or knee revision who were admitted to the Department of Orthopaedics of the Second Affiliated Hospital of Zhejiang University School of Medicine with large bone defects from July 2018 to December 2023 were retrospectively analyzed, including 4 patients with TKA and 3 patients with knee revision. There were 3 males and 4 females, aged 58.7±7.6 years (range, 54-68 years), 3 patients with left knee and 4 patients with right knee. All the patients had bone defects in the knee joint (AORI type III), 2 cases had bone defects only in the femur, 4 cases had bone defects only in the tibia, and 1 case had bone defects in both the tibia and femur, which were treated with personalized reconstruction using 3D printing. Hip-knee-ankle angles, American Knee Society score (KSS) before and after surgery were compared, and postoperative complications were observed.Results:All patients successfully completed the operation, and the operation time was 189.3±35.5 min (range, 125-240 min). Complex TKA was performed in 4 cases with surgical times of 175, 195, 210, and 240 min, and revision surgery was performed in 3 cases with surgical times of 125, 180, and 200 min, respectively. Intraoperative blood loss was 114±24.4 ml (range, 100-150 ml). Five cases used 3D printed metal augment, and two used 3D printed one-piece tibial components. All patients were followed up for 2, 2, 5, 6, 7, 20, 57 months, respectively. The KSS of the five patients at 3 months postoperatively were 56, 61, 66, 56, and 56 points, respectively, greater than the preoperative scores of 35, 44, 36, 27, and 41 points. The KSS functional scores of the five patients at 3 months postoperatively were 45, 45, 45, 30, and 45 points, respectively, which were greater than the preoperative scores of 30, 30, 15, 20, and 20 points. The hip-knee-ankle angle was 181.8°±3.4° (range, 177.9° to 188.0°) at the final follow-up and 175.8°±12.4° (range, 153.3° to 192.1°) before surgery, with no significant difference ( t=-1.230, P=0.242). At the final follow-up, the 3D printed component was well integrated with the bone surface, the prosthesis was securely positioned, and the force lines of the lower limbs were normal. There were no postoperative complications such as poor wound healing, infection, fat liquefaction, nerve injury, deep vein thrombosis of lower limbs, knee joint stiffness, periprosthesis infection and loosening. Conclusion:Using 3D printed metal augment or tibial prosthesis to reconstruct the huge bone defect in TKA and revision has a satisfactory early clinical effect, satisfactory joint function and good surgical safety.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.

Objective To discuss the effectiveness and safety of transarterial chemoembolization(TACE)combined with regorafenib and programmed death receptor-1(PD-1)in the second-line sequential treatment of advanced hepatocellular carcinoma(HCC).Methods The clinical data of a total of 83 patients with advanced HCC,who received TACE combined with regorafenib and PD-1(triple-therapy group)or TACE combined with regorafenib(dual-therapy group)at the Affiliated Hospital of Nantong University and Nantong Municipal Third People's Hospital of China between October 2020 and May 2022,were retrospectively analyzed.The clinical data were collected and evaluated.Modified response evaluation criteria in solid tumors(mRECIST)was used to evaluate the curative effect.The progression-free survival(PFS),overall survival(OS)and treatment-related adverse events(TRAEs)were compared between the two groups.The Kaplan-Meier method was used to draw PFS and OS curves,the Log-rank test was used to compare the relevant data between the two groups,and the COX regression model was drawn to determine the factors influencing PFS and OS.Results There were no statistically significant differences in the baseline data between the two groups(P≥0.05).In the triple-therapy group and the dual-therapy group,the objective response rate(ORR)was 31.1％and 18.4％respectively(P=0.024),and the disease control rate(DCR)was 77.8％and 57.8％respectively(P=0.038).The OS and PFS in the triple-therapy group were higher than those in the dual-therapy group(16.80 months vs 13.20 months,and 9.10 months vs.7.40 months,respectively).No statistically significant difference in the incidence of adverse drug reactions existed between the two groups(P 0.05).Conclusion In the second-line sequential treatment of advanced HCC,TACE combined with regorafenib and PD-1 is more effective than TACE combined with regorafenib,therefore,it can be used as a preferred second-line treatment for advanced HCC.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.

In the context of the high-quality development of public hospitals,it focuses on the task of improving the level of comprehensive budget management,with the difficulties and challenges in budget management in a certain hospital,it explores the path of building a smart financial system to serve comprehensive budget management.The practical application of the system has shown that information technology has effectively improved the budget management level of medical use.At a deeper level,case studies on the construction of the intelligent financial system demonstrate the importance of information technology in assisting hospitals with comprehensive budget management,elucidating the significance of information technology in improving the hospital budget management level and promoting high-quality development.