Objective To investigate the effect of nesfatin-1 (NSF-1) on T-type Ca2+ channel currents in adult mouse dorsal root ganglion (DRG) neurons and possible signal transduction mechanisms involved.Methods We measured the expression of melanocortin 4 receptors(MC4-R)in mouse DRG by using western blotting analysis.The whole-cell patch clamp technique was used to record the effects of NSF 1 on T-type Ca2+ channel currents in small DRG neurons and several ligands were experimented to further clarify relevant signaling pathways.Results MC4-Rs were abundantly expressed in DRG neurons.NSF-1 enhanced T-type calcium channel currents in a dose-dependent manner in small DRG neurons in mice.NSF-1 mediated increment of T-type calcium channel currents was blocked by the MC4-R antagonist HS024,phosphokinase C antagonists GF109203X,and chelerythrine chloride,while the blockade of phospohokinase A PKI 6-22 elicited no such effects.Conclusions NSF-1 can enhance T type calcium channel currents in small DRG neurons through an MC4-R-dependent PKC signaling pathway.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

Objective To analyze the effect of Mortality and Morbidity Conferences(MMC)in the prevention and treatment of hospital-related venous thromboembolism(VTE),and to provide a reference forthe implementation of prevention and treatment measures of VTE in hospitals.Methods Two MMC implemented in August-September 2023 at a tertiary genera l hospita l in Beijing were selected,and rainstorming and root cause analysis were used to profile two cases selected at the meetings that were highly suspected to have died from acute pulmonary embolism.Results Insufficient awareness of VTE,insufficient sensitivity to the diagnosis of VTE,inadequate management of adverse events and failure to establish a VTE risk assessment process are the root causes of hospital-related VTE events.Conclusion Hospitals should establish a management organisational structure for the prevention and treatment of VTE,carry out VTE training on a regular basis,set up a VTE information system,define a standardised VTE risk assessment process,and enhance the sensitivity of the medical staff to the patient's abnormalities.

The teleconsultation model of the National Telemedicine and Connected Health Center is cited as an example, to study the pricing formulation of video teleconsultation. This refers to the pricing composed of such four parties as the inviter′s hospital, invitee′s hospital, technical support, and business operation. Based on such pricing standard, teleconsultation charges can be collected. From 2015 to 2017, the annual applications of teleconsultation is on the rise year by year. Such pricing has been accepted by patients at large, as it embodies the value of each party, and ensures the long-term running of telemedicine system. Therefore, this pricing formulation could be a referable model for the localities to formulate their teleconsultation pricing.