This study was carried out to defect the effects of acupuncture and moxibustion therapy on the proliferation and differentiation of neural stem cells in the olfactory bulb of senescence-accelerated mouse P8. Immunofluorescence double staining methods(BrdU/Nestin, BrdU/MAP-2 and BrdU/beta-tubulin III) were used. The results show that both acupuncture and moxibustion could improve proliferation of neural stem cells, but only moxibustion could induce differentiation of neural stem cells into neuron.
Acupuncture Therapy ; Animals ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Male ; Mice ; Moxibustion ; Neural Stem Cells ; cytology ; Olfactory Bulb ; cytology