Objective To investigate the important role of bronchial provocation test with hypertonic saline and induced sputum in the diagnosis and treatment of chronic nonproductive cough by assessing its feasibility and the side effects which may occur during the procedure.Methods The diagnosis procedure was based on the anatomical protocol for diagnosing chronic nonproductive cough,howerer,hypertonic saline bronchial provocation test and induced sputum were conducted for unconfirmed 38 pafients who suffered chronic congh.Results 23(23/38,60.5%)patients with airway hyperresponsiveness,the forced expiratory volume in one second(FEV1)was decreased 27%?5.8)%,Side effects occurred in 3 patients(3/38,7.8%).Appropriate sputum samples were obtained after hypertonic saline challeng,The percentage of eosinophi1 counts in induced sputum was high in 24 patients(24/38,63.2%),including cough variant asthma(CVA)(17/23,73.9%)and eosinophilic bronchitis(EB)(7/38,18.4%).After treatment,the percentage of eosinophi1 counts in induced sputum of patients was significantly reduced in CVA and EB.Conclusion Bronchial provocation test with hypertonic saline and induced sputum were an effective method for diagnosing chronic nonproductive cough and little side effects occurred during the procedure.

The nucleotide-binding oligomerization domain(NOD)proteins are recently identified as cytosolic pattern recognition receptors located on innate immune and epithelial cells,and recognize pathogen-associated molecular patterns.The NOD1 and NOD2,the two members of NOD protein family,have important roles in innate immunity as sensors of microbial components derived from bacterial peptidoglycan.Besides,NOD proteins have been established as key regulators of cell death and cytokine production,they also have been shown to participate in the pathogeny of allergic disease such as asthma.This review will focus on the identification and functional characterization of NOD proteins,as well as their role in lung diseases.

Objective To compare the application effect between the method of cassette automatic blood analyzer and the method of traditional serological .Methods Using the method of cassette automatic blood analyzer and the method of traditional serological , respectively ,the samples of blood donors in Dongguan from October 1 ,2013 to April 30 ,2014 were performed irregular antibody screening .The positive samples of screening were identified by using antiglobulin method ,and the irregular antibodies of blood do‐nors were analyzed .Results There were 95 positive cases of irregular antibody by the method of cassette automatic blood analyzer , and the detection rate was 0 .208% .There were 16 positive cases of irregular antibody by the method of traditional serological ,the detection rate was 0 .035% ,and there was statistical significance in differences (P< 0 .05) .The positive coincidence rate of the method of cassette automatic blood analyzer was 56 .547% ,higher than the rate of the method of traditional serological which was 28 .571 % (P<0 .05) .The positive rate of irregular antibody was 2 .242% in RhD‐negative blood donors ,higher than the rate in RhD‐positive blood donors which was 0 .198% (P<0 .05) .Conclusion The positive rate of irregular antibody in blood donors i‐dentified by using the method of cassette automatic blood analyzer is higher than the rate identified by using the method of tradition‐al serological .The irregular antibody screening should be performed for RhD‐negative blood donors .The types of irregular antibody in blood donors are mainly the types of IgM which are no clinical significance .

OBJECTIVETo study the differences of HPLC fingerprints with the total quantum statistical moment for the Buyang Huanwu decoction (BYHWD) processed by various ways, and to verify the additive properties of total quantum statistical moment.

METHODThe extracts of BYHWD were obtained by water extraction and alcohol precipitation firstly, and then were dissolved with five solvents of different solubility parameter from 11.4 Cal(1/2) x cm(-3/2) to 23.40 Cal(1/2) x cm(-3/2) respectively. Their chromatographic fingerprints were determined by HPLC, finally the total quantum statistical moment parameters and its superposition properties were manual calculated and analyzed by their expressions.

RESULTAs a contrast as the whole prescription, the similarities of the various processed samples with butanol (11.4 Cal(1/2) x cm(-3/2)), methanol (13.5 Cal(1/2) x cm(-3/2)), 68% methanol (16. 67 Cal(1/2) x cm(-3/2)), 34% methanol (20.03 Cal(1/2) x cm(-3/2)) and distilled water (23.40 Cal(1/2) x cm(-3/2)) were 0. 074, 0. 973, 0. 934, 0. 991, 0. 993, respectively and while the RSD of these total quantum zero moment, center moment and variance of each chromatographic fingerprints for them were 63.04%, 16.22%, 69.38%, which showed significant difference in these chromatographic fingerprints. The total quantum statistical moment parameters of the superimposed chromatographic fingerprint with each sole samples were 3.203 x 10(5) mAu x s, 29.85 min, 389.97 min2, whereas the whole prescription's were 6.548 x 10(4) mAu x s, 29.44 min, 389.00 min2, that suggested that the absolute difference percentages between the superimposed and the whole were 2.209%, 1.389%, 0.2484%, respectively.

CONCLUSIONThe total quantum statistical moment of the chromatographic fingerprints is of characteristics with additive properties, it can be used in static and dynamic quality controlled analyses in the Chinese medicine multiple component systems.

Biometry ; methods ; Chromatography ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Solubility

Sepsis is defined as life-threatening organ dysfunction caused by a dysregulated host response to infection.Proper endocrine adjustment is required for self-protection of the body during stress conditions; however, decompensation is harmful.It has been proved that violent stress response caused by sepsis can lead to endocrine decompensation and is harmful to children during clinical course and prognosis.

OBJECTIVETo explore the once sampling quantitation of Houttuynia cordata through its DNA polymorphic bands that carried information entropy, from other form that the expression of traditional Chinese medicine polymorphism, genetic polymorphism, of traditional Chinese medicine.

METHODThe technique of inter simple sequence repeat (ISSR) was applied to analyze genetic polymorphism of H. cordata samples from the same GAP producing area, the DNA genetic bands were transformed its into the information entropy, and the minimum once sampling quantitation with the mathematical mode was measured.

RESULTOne hundred and thirty-four DNA bands were obtained by using 9 screened ISSR primers to amplify from 46 strains DNA samples of H. cordata from the same GAP, the information entropy was H=0.365 6-0.978 6, and RSD was 14.75%. The once sampling quantitation was W=11.22 kg (863 strains).

CONCLUSIONThe "once minimum sampling quantitation" were calculated from the angle of the genetic polymorphism of H. cordata, and a great differences between this volume and the amount from the angle of fingerprint were found.

Amplified Fragment Length Polymorphism Analysis ; DNA, Plant ; Genetic Variation ; Houttuynia ; genetics ; Microsatellite Repeats ; genetics