Objective To compare the content of organic acid in Folium Isatidis from differernt Habitats. Methods According to ultraviolet absorption atlases characteristics of the three main organic acid in Folium Isatidis, DSA UV-2401 was used to determinate the acidcontent of Folium Isatidis from differernt Habitats, and carried on to inspect the methodology. Results The difference of the acidcontent of Folium Isatidis from differernt Habitats is notable. Conclusion The method is simple, accurate and duplicated good, and can be used as quantitative analysis method of organic acid in Folium Isatidis.

BACKGROUND: The wear resistance and hardness of artificial tooth would affect repairing effects in clinic. However, there is lack of professional standard for resin teeth in China.OBJECTIVE: To provide a theoretic foundation for the choosing of the resin teeth in clinic via comparing the wear resistance and hardness among 7 different resin teeth.DESIGN, TIME AND SETTING: A contrast observation. The experiment was performed at the laboratory of Mechanical Engineering College, Shanghai Jiao Tong University from November 2003 to May 2004.MATERIALS: A total of 7 kinds of different resin teeth were selected, namely, hard multi-layer synthetic resin teeth (A); multi-layer synthetic resin teeth (B); synthetic resin teeth (C); EMDURA resin teeth (D); three-layer synthetic resin teeth (E); optostar four-layer synthetic resin teeth (F); and Cosmo HXL (G).METHODS: The wear test was processed by a pin-plate wear machine. The result was measured by the weight loss through wearing and the surfaces of the wear scar were observed by a scanning electronic microscope (SEM); hardness was measured by Knoop hardness. Meanwhile, the relativity between the hardness and wear resistance of resin teeth was analyzed.MAIN OUTCOME MEASURES: The wear resistance and hardness of resin teeth.RESULTS: According to the weight loss and SEM examination, the wear resistance of the different resin teeth was D>A>G>E>B>F>C; and the hardness of different resin teeth was D>A>G>E>F>B>C. The correlation coefficient between the weight loss and hardness was -0.888 (P < 0.01).CONCLUSION: The variation of the wear resistance of the resin teeth may influenced by their different components, molecular weight and manufacturel methods. There are positive correlation between the hardness and wear resistance of resin teeth.

Objective To evaluate the antigenic specificity of the enolase.Methods and Results The results included:(1)Western blot analysis demonstrated that enolase was a main protein in the nonglyco-proteins of C.albicans which could react with the serum of C.albicans infected rabbit.(2)The purified enolase was used to immunize rabbit for producing anti-enolase serum,and the hig hest titer of polyclonal antibody against enolase was up to 1:6400with enzyme-linked immunoadso rbent assay(ELISA).(3)Compared with three other polyclonal antibodies,anti-enola se antibody showed high specificity in detecting C.albicans antigen.Conclusion Enolase as a specific antigen,is worthy of further investigation in C.albicans cell.

Objective To assess three-dimensional color Doppler energy (3D-CDE) in the diagnosis of liver tumors.Methods Vascular pattern of 2D- and 3D-CDE were used to analyze 106 tumors of 96 patients,and their sensitivity and specify were calculated respectively.Three-dimensional volumetric measurement was used to calculate the volume,vessel counts of tumor,as well as the ratio of vessel counts and volume,whereas vascular index (VI) of both benign and malignant lesions were assessed.Results ①Using the vascular pattern of IE as diagnosis standard of liver malignant,the sensitivity and specificity of 2D-CDE and 3D-CDE were 21.33%,100% and 81.33%,100%,respectively) using the vascular pattern of II and Ⅲ,the sensitivity and specificity were 73.33%,67.74% and 100%,45.16%,respectively.②Mean VI was 0.38 counts/cm~3 of malignant tumors and 0.18 counts/cm~3 of 31 benign tumors (P<0.05),The sensitivity and specificity in diagnosing malignant tumors of liver was 78.67% and 87.10%,respectively with VI>0.30 counts/cm~3.Conclusion The vascular pattern of 3D-CDE HI and VI>0.30 counts/cm~3 may be taken as parameters for the diagnosis of malignant liver tumor.

Objective:To study the clinical effect of local application of Gengigel gel (0.8%hyaluronic acid gel) in the treatment of plaque-induced gingivitis.Methods:30 volunteers with plaque-induced gingivitis were included. At least two molars and/or premolars in each quadrant of oral cavity in each subject were treated by local application of Gengigel gel adjunctive to sacling (group SG), scaling alone (group S), local application of Gengigel gel (group G) or without any treatment (group C ) respectively. Plaque index(PLI), gingival index(GI) and sulcus-fluid-flow-rate(SFFR) were monitored before treatment, 4 and 7 days after treatment.Results:GI and SFFR in group SG decreased significantly faster than those in the group S (P

Objective To study the specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalian. Method The specific sequences in the 3'untranslated region (UTR) of the SON gene of human beings and 14 kinds of mammalians were amplified and directly se-quenced. The sequence data of PCR products of human beings and mammalians were declared. Results The sequences of 3'UTR of SON gene of human beings differed from 14 kinds of mammalians. The sequences of samples came from different human individuals did not show any difference. Conclusion The 3'UTR of SON gene is a good DNA region for specie identification. Using this technology, DNA of human beings can be distinguished from 14 kinds of mammalians. This technology is very useful in of forensic science.

Objective To investigate the characteristics and possibility of using an image analyzer-aided method to count axotomized retinal ganglion cells (RGCs). Methods The left optic nerves of 18 rats were transected intraorbitally and a piece of gelform soaked in 5% fluorogold was applied to the ocular stump to retrogradely label the surviving RGCs. All animals were executed 2, 7 or 14 days after the operation (n=6 for each time point), respectively. The left retinae were removed, post-fixed and whole-mounted on the slides. The numbers of labeled RGCs were counted using both the conventional sampling method and image analysis, and compared statistically between the two methods. Results The number of surviving RGCs decreased sharply [(12 0663?9 089), (59 285?17 071) and (17 802?19 84) cells/mm 2 for image analyzer-aided method, and (118 237?7 898), (57 648?14 533) and (18 070?1 461) cells/mm 2 for conventional sampling method] when the survival time increased from 2 to 7 and 14 days. No significant difference was detected between the two groups at any corresponding time points. Conclusion The image analyzer-aided method is convenient, objective and reproducible, which can be used in the studies where counting RGCs is needed.

Objective To study the inhibitory effect of ibandronate(IBN)on the growth of lung cancer cell line PLA-801D in vitro,and the mechanism of such effect was studied.Methods The effects of different doses or treatment time of IBN on the growth of PLA-801D cell line were detected by MTT method.The mechanism of the effect was analyzed by the examination of cell cycle using flow cytometry.Results With the concentration of 10 to 100 ?g/ml,IBN obviously inhibited the growth of PLA-801D cell(P

A variety of opportunistic infections can occur in the late-stage of HIV-1 infection,Mycobacterium avium complex (MAC) infection is a common cause of disseminated bacterial infection. Since the advent of highly active antiretroviral therapy (HAART),the rate of MAC infection has declined substantially,but there is risk in individuals with low CD4 cell counts. This article describes the epidemiological,clinical,therapeutic and preventive aspects of MAC infection in HIV-sero-positive individuals.

Objective To evaluate the feasibility and efficacy of 125 I radioactive seed implantation in treat-ment of aggressive thymoma pleural metastases after surgical resection.Methods The clinical data of 16 patients with thymoma confirmed underwent surgery were retrospectively analyzed.All post -operative pleural metastasis were trea-ted by radioactive particle planting.Results No patients occurred serious complications.15 patients survived at the end of 12 months following -up,13 patients with complete or partial relieved,2 patients progressed and 1 patient lost to following -up.Conclusion DynaCT -guided 125 I radioactive seed implantation is effective and safe for treating aggressive thymoma pleural carcinomatosis,it offers a new and feasible treatment.