Objective To explore the inhibitory effects of the vector on the growth of mammary adenocarcinoma cell MCF-7 and human telomerase reverse transcriptase(hTERT) gene expression in MCF-7 cells,by the way of the construction of the RNA interference(RNAi) expression vector of hTERT gene.Methods An interference target sequence TGT TCA GCG TGC TCA ACT A,which aimed at hTERT gene,was designed and the small interfering RNA(siRNA) expression plasmid pGenesil-hTERT was constructed by means of recombination.The recombinant plasmid was transfected into the MCF-7 cells by LipofectamineTM 2000 after the identification of the recombinant expression vector pGenesil-hTERT in the light of electrophoresis after digestion and DNA sequencing,then RT-PCR analysis was used to detect the gene expression,and Western blotting analysis was used herewith to detect the protein expression of hTERT,and the growth of MCF-7 cells was tested by MTT assay.Results The results of analysis and the sequencing identification confirmed that the target sequence was inserted into the predicted site precisely,and the recombining plasmid was successfully constructed.RT-PCR and Western blotting analysis showed that hTERT gene expression and protein expression of pGenesil-hTERT group were obviously decreased in MCF-7 cells,and the growth of MCF-7 cells of pGenesil-hTERT group was also inhibited.Conclusion The endogenous hairpin siRNA produced by DNA plasmid is capable of mediating hTERT gene inhibition in MCF-7 cells effectively,and furthermore inhibiting the cells growth and decreasing the speed of cells proliferation.This research may act as the experiment evidence to the gene therapy of cancer.

Objective To evaluate the morphological changes of the small nerves in lower extremity with high-resolution ultrasonography in type 2 diabetes mellitus (T2DM). Methods 84 cases of T2DM patients were divided into four different groups according to TCSS score [group Ⅰ (0-5 point), group Ⅱ (6-8 point), groupⅢ (9-11 point), group Ⅳ (12-19 point)]. The ultrasonic feature of posterior tibial nerve, sural nerve and saphenous nerve were observed to observe the relationship between ultrasound findings and scoring. Results Abnormality rate admitted from ultrasound findings are as follows. In group Ⅳ, nerve abnormality rate was the highest, followed by group Ⅲ, group Ⅱ and group Ⅰ (P < 0.05). The major manifestations are obscure border between spineurium and peripheral tissue , nerve bundle mesh like structure disappearing , enlargement of posterior tibial nerve and sural nerve. There was no statistical difference in irregular abnormality rate (P > 0.05). The saphenous nerve epineurium abnormality increased in group Ⅱ and Ⅲ compared with group Ⅰ and Ⅳ(P <0.05). Abnormality rate of 3 neural ultrasound decreased in line with sural nerve, posterior tibial nerve and saphenous nerve. Conclusions Certain correlation was observed between TCSS and never ultrasonic feature in T2DM patients. The higher TCSS scores is, the greater chance of presence of abnormal sonographic features of nerves will be. The sural nerve abnormality was higher than others.

Objective: To compare the extraction efficiency of imperialine extracted with different solvents. Methods: The content of imperialine in the extracts was determined by thin layer chromatography scanning. Results: Among the methods suitable for alkaloid extraction, the solvent ether chloroform 95% ethanol (25∶8∶2.5) is the best for imperialine extraction. Conclusion: The imperialine extraction efficiency is greatly affected by the extraction solvent.

Objective To explore the optimized scanning parameters of low-dose un-enhanced chest CT with sinogram affirmed iterative reconstruction (SAFIRE).Methods Eleven healthy adult domestic dogs underwent chest scanning of 120 kV,100 kV,80 kV,70 kV with a fixed tube current of 65 mA and halved tube current of 32 mA with a fixed tube voltage of 100 kV.The data were reconstructed with filtered back projection (FBP)and SAFIRE (strength grade 3)respectively to obtain ten groups of images.The regular dose (120 kV/65 mA ) combining with FBP reconstruction was as normal control group,and other groups as experimental groups.CT dose index (CTDI), dose length product (DLP),and effective dose (ED)at different dose were compared,and average CT value,noise,signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR)and image quality score of ten groups of images were comparatively analyzed.Results No significant differences were found in average CT value for lung tissue,the superior vena cava,the aortic arch,and spinal muscles among ten groups (P >0.05),except adipose tissue (P <0.05).Compared to the control group,radiation dose of the 100 kV/65 mA-SAFIRE group decreased by 40.00%,noise of this group reduced,SNR and CNR increased,image quality score was slightly higher.Radiation dose of 80 kV/65 mA and 100 kV/32 mA groups,compared to the control group,decreased by 71.33% and 70.00% respectively ;image quality was slightly degraded but the subjective image quality scores were above 4 ,which on the latter group was slightly higher than that on the former.Image quality of 70 kV/65 mA group was reduced but could meet the requirements of diagnosis (image quality score >3),and the radiation dose of this group decreased by 81.33%.Conclusion Reconstructed with SAFRIE technology,the image quality of 100 kV/65 mA scanning of chest CT is similar to that of routinely reconstructed images in regular dose,while the image quality of 70 kV/65 mA scanning can not only meet the requirements of diagnosis but also obviously reduce radiation dose.

ABSTRACT:In this study ,we elucidated the predictors of progression to liver failure during severe acute exacerbation .We analyzed 69 consecutive patients with severe acute exacerbation of chronic hepatitis B for clinical outcome and factors that influ‐enced the development of liver failure ,including viral genotype ,PC (G1896A) and BCP (A1762T/G1764A) mutants .Thirty‐three (47 .8% ) severe acute exacerbation patients progressed to liver failure .Multivariate analysis identified serum bilirubin (TB>256 μmol/dL ,P=0 .008) and prothrombin activity (PTA<40% ,P<0 .001) as significant determinants of progression to liver failure .HBeAg negativity (P=0 .065) and PC mutant (P=0 .090) were associated with the progression to hepatic de‐compensation .Serum total bilirubin ,prothrombin activities ,HBeAg status and PC mutant were predictors of clinical outcome in patients with severe acute exacerbation of chronic hepatitis B .

Objective To explore the effect of clinical nursing path (CNP) on stroke patients. Methods 80 stroke patients were divided into control group and experimental group with 40 cases in each group. The control group received functional nursing, while the experimental group received care in accordance with the design of CNP map. Results The mastery rate of health knowledge and the satisfaction rate were higher, and the hospitalization time was shorter in the experimental group than in the control group (P<0.05). Conclusion The implementation of CNP could increase the mastery degree of health knowledge, improve the satisfaction rate of patients for nursing service, and shorten the hospitalization time.

Excellent doctor education training plan is an important policy applied by the gov-ernment to enhance the quality of medical personnel training in China. One of the important purposes is to improve the medical students' ability to apply and practice the medical professional English. In order to achieve this purpose, we have applied bilingual teaching for the students of excellent doctor in medical cell biology course. This article mainly introduces some reform measures , which involves training teacher, preparing textbooks and resources, establishing network database, exploring teaching modes, improving teaching methods and means, implementing diversified evaluation methods, and so on. At the same time, we have summarized some problems in the teaching process, and thus put for-ward improvement strategy.

ObjectiveTo observe the outcomes of psychological nursing for earthquake patients in recovery stage. Methods90 earthquake patients were divided into 4 groups by age and accepted psychological nursing. They were evaluated for post-traumatic stress disorder (PTSD) a year after earthquake. ResultsNone PTSD had been found. ConclusionPsychological nursing can improve self-care ability and confidence of the earthquake patients.

Objective To explore the effect of Schisandrin B (Sch B) on nuclear transcription factor E2-related factor 2 (Nrf2)/kelch-like epichlorohydrin-associated protein (Keap-1)/ peroxisome proliferation-activated receptor γ coactivator-1α (PGC-1α) signaling pathway in lung tissue of rats with severe pneumonia. Methods A rat model of severe pneumonia was first established and then randomly divided into model group, Sch B low, medium, and high dose groups and positive control group, with 10 rats in each group, and another 10 rats was selected as a blank control group. Sch B low, medium and high dose groups were given intragastrically with 2.50, 5.0, 10.0 mg/kg Sch B for intervention, the positive control group was given 1.04 mg/kg dexamethasone for intervention, and the rest of groups were given equal volume of normal saline, for 14 consecutive days. Aorta blood was taken to detect blood gas index. Lung tissue was isolated, and pathological changes, inflammatory factors and pathway-related protein expression were detected. Results The rats in the control group had normal diet, no abnormal mental state, and clear lung tissue structure. Compared with the control group, the rats in the model group were in a worse state, with symptoms such as unresponsiveness, sluggishness, and shortness of breath, inflammatory infiltration of the lung tissue, edema of the alveolar interstitium, and thickening of the alveolar wall. The PaCO2 value, TNF-α, IL-6 and IL-1β contents, Keap-1 protein expression all increased significantly (P<0.05), the PaO2 and SaO2 levels, Nrf2 and PGC-1ɑ protein expression reduced significantly (P<0.05). Compared with the model group, the adverse symptoms of rats in the Sch B low, medium, and high dose groups alleviated gradually, and the inflammatory infiltration of lung tissue and alveolar interstitial edema reduced gradually, the PaCO2 value, TNF-ɑ, IL-6 and IL-1β contents, and Keap-1 protein expression all decreased sequentially (P<0.05), the PaO2 and SaO2 values, Nrf2 and PGC-1ɑ protein expression levels increased sequentially (P<0.05). The indicators were no significant difference between the Sch B high-dose group and the positive control group (P>0.05). Conclusions Sch B can alleviate the adverse symptoms of severe pneumonia caused by Klebsiella in rats, which may be related to the activation of the NRF2/PGC-1α signaling pathway and the reduction of Keap1 protein expression.

OBJECTIVE:To systematically review the efficacy and safety of the Bushen huoxue lishi category TCM compound in the treatment of chronic prostatitis,and provide evidence-based reference for clinical treatment. METHODS:Retrieved from VIP Database,Wanfang Database,CJFD and CBM,randomized controlled trials(RCT)about Bushen huoxue lishi category TCM com-pound preparation (test group) versus conventional Western medicine (control group) in the treatment of chronic prostatitis were collected. Meta-analysis was performed by Rev Man 5.3 software after data extraction and quality evaluation. RESULTS:Totally 22 RCTs were enrolled,involving 1 863 patients. Results of Meta-analysis showed the total effective rate [OR=4.46,95%CI(3.40, 5.84),P<0.001],total scores of chronic prostatitis symptoms[MD=-3.62,95%CI(-5.21,-2.04),P<0.001] and lecithin count [MD=7.58,95%CI(2.15,13.01),P=0.006] in test group were significantly higher than control group,prostatic fluid white blood cell count [MD=-1.68,95%CI(-3.26,-0.10),P=0.04] was significantly lower than control group,with significant differenc-es. CONCLUSIONS:Bushen huoxue lishi category TCM compound has good efficacy in the treatment of chronic prostatitis.