Eucalyptus globulus can pbviously reduce edematous swelling of ear,inhibit the proliferation of granuloma,and blood capillary permeability of mice. It can also obviously inhibit cotton pellet granuloma implanted inrats. It has obvious anti-inflammatory and analgesic effect on arthritis in rat induced by carrageenan and painreaction in mice induced by Woolfe-Macdonald as well as HAc-induced writhing.

ObjectiveTo observe the effects and safety of combination of Ozagrel and low molecular weight heparin and rehabilitation training in the treatment of progressive cerebral infraction. Methods60 patients with progressive cerebral infraction were divided into the observation group and the control group with 30 patients in each group. 80 mg Ozagrel plus 250 ml normal saline infusion were used in the observation group, once every 12 hours, 14 days for a course of treatment, 0-4 ml low molecular weight heparin was used with subcutaneous injection every 12 hours interval time, 14 days for a course of treatment. But only Ozagrel was used in the control group. The same treatment was used in the two groups, all patients received routine rehabilitation training. Platelet and activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (Fg) were examined before and 14 days after treatment. The NIHSS score was assessed.ResultsAfter treatment, NIHSS score in both groups decreased significantly(P<0-01) while the observation group was significantly better than the control group (P<0-01). The efficiency of the observation group was 86-7%, the control group was 60-0%, the observation group was significantly higher than the control group(P<0-05). Platelet count, PT, APTT, and Fg of the two groups were in the normal range, and there was no obvious adverse reaction.ConclusionOzagrel and low molecular weight heparin and rehabilitation training is safe and effective in the treatment of progressive cerebral infraction.

Objective To observe the effect of continual rehabilitation on patients with brainstem infarction. Methods 52 stroke patientswith brainstem infarction were divided into early rehabilitation group (n=26) and continual rehabilitation group (n=26). 2 groups receivedearly rehabilitation for 1 month. Then the continual rehabilitation group continued rehabilitation in the rehabilitation unit, while the early rehabilitationgroup just received the medical guide after discharge. Fugl-Meyer Assessment (FMA), the Balance subscale of the Fugl-Meyertest (FM-B), and modified Barthel Index (MBI) were applied to assess the motor function, balance function and the activities of daily living(ADL) in two groups respectively before and 1 month and 3 months after training. Results The motor function, balance function, the performanceof activities of daily living improved in the continual rehabilitation group (P<0.05). Compared with the early rehabilitation group, thecontinual rehabilitation group significantly improved in FMA, FMA-B and MBI 3 months after the rehabilitation (P<0.05). There was a positivecorrelation in the scores among FMA and FMA-B and MBI. Conclusion The continual rehabilitation is benefit to the motor functionand balance function and ADL of the patients with brainstem infarction.

Primary carcinoma of the gallbladder (PCG)is the common malignant tumor in the biliary system.Because there is no specific clinical manifestation, patients diagnosed with PCG are mostlyin intermediate or advanced stages.Therefore, they lose the chance of radical resection of gallbladder carcinoma and have a poor prognosis.Radiotherapy and chemotherapy are not effective palliative measures.Immunotherapy has become an alternative strategy besides operation, chemotherapy and radiotherapy.Treatment with DCs vaccines, as a new method of immunotherapy, has become more and more important.Here we review treatment with DCs vaccines in primary carcinoma of gallbladder.

OBJECTIVE:To investigate the inhibitory effect of astragalus polysaccharide(APS)on the proliferation of human neuroblastoma SH-SY5Y cells. METHODS:After the cells were cultured with 0(blank control),25,50 and 100 mg/ml APS for 6,12 and 24 h,MTT method was used to determine cell viability and calculate inhibition rate. Following cell cultured with 0 (blank control),25,50 and 100 mg/ml APS for 24 h,Hoechst 33258 fluorescent staining was performed,and then cell nucleus morphology was observed under the fluorescence microscope;flow cytometer was used to detect the distribution of cell cycles and apoptosis;western blot was employed to determine the expression of extracellular regulated protein kinases (ERK) 1/2 protein in cells. Enzyme linked immunosorbent assay (ELISA) was conducted to determine the contents of interleukin 2 (IL-2),IL-6 and IL-12 in the cells. RESULTS:Compared to the blank control,those cultured with 100 mg/ml APS for 6 h,50 and 100 mg/ml APS for 12 h and 25,50 and 100 mg/ml APS for 24 h demonstrated higher inhibition rate. After the cells were cultured with 50 and 100 mg/ml APS for 24 h,those in G0/G1 phase increased and those in G2/M and S phases decreased,and the contents of IL-2 and IL-6 increased. After cells were cultured with 25,50 and 100 mg/ml APS for 24 h,the apoptosis rate was higher,densely hyperchromat-ic fragments in cell nuclei and apoptotic bodies appeared,the phosphorylation level of ERK1/2 protein in the cells was lower,and the content of IL-12 was higher. There was statistically significance (P<0.01 or P<0.05). CONCLUSIONS:APS can inhibit the proliferation of SH-SY5Y cells by arresting cell cycle and inducing cell apoptosis through a mechanism which may be correlated to the decrease in the phosphorylation of ERK1/2 and increase in cytokine.

Objective To evaluate the value of bispectral index(BIS)monitoring as an indicator for extubation sedation level after children's oper?ation by total intravenous anesthesia. Methods One hundred and eighty children(2?13 years old)were randomly divided into six groups with 30 cases in each. Group A kept BIS 56?60,and Group B 61?65,Group C 66?70,Group D 71?75,Group E 76?80,Group F 81?85 till extubation. All children were given fentanyl,propofol and cisatracurium besilate when induced,and maintained with propofol and remifentanil. All cases kept BIS 40?55 during the operation. Then the changes of electrocardiogram,mean arterial pressure,heart rate,SpO2,postoperative complications and recovery time were observed. Results Groups A and B were extubated at deep anesthesia,cycle stability,but with high incidence of adverse reactions and awaked with a long time. Groups E and F were extubated when awaked,but with greater stimulation and easier agitation. Groups C and D were lighter hemodynamic responses,less respiratory effects and less postoperative complications of anesthesia. Conclusion The BIS level of 66?75 is a good se?dation level for extubation,especially in the level of 71?75.

Objective To study the optimal operation procedure for elderly patients with cholelithiasis. Methods 1395 elderly patients with cholelithiasis,who were more than 65 years old,were treated with operation.1356 cases of them were treated with mini-cholecystectomy(MC). Results MC were performed on 1356 patients successfully(97.2%).The incidence of the operative complication was 2.58%(35/1356).No death occurred.The mean hospital stay was 6.2 days. Conclusions Elderly patients with cholelithiasis must be operated on as early as Possible.Because of minimal invasion and quicker recovery after operation,MC will be the first choice for the treatment of elderly patients with cholelithiasis.

Objective To observe the effect of rosiglitazone on serum NOS/NO in apolipoprotein(apo) E knockout mice.Methods Twenty eight-week-old apoE knockout mice were intragastrically administrated 0.2 ml 5% sodium carboxymethycellulose for 12 weeks to establish the animal models of atherosclerosis,in which half of mice simultaneously received 10 mg?kg~(-1)?d~(-1) rosiglitazone as treatment.Ten wildtype mice were used as the normal control group.All mice were fed on normal chow diet.After 12 weeks,aorta were used for histomorphometric analysis by means of HE.Vessel blood was collected for plasma lipid,NO and NOS.Results Histomorphometric analysis showed that the area of atherosclerosis plaque in mice receiving rosiglitazone was significantly smaller than the mouse models of atherosclerosis,while the plasm lipid,NO and NOS were higher.Conclusion Rosiglitazone can inhibit the development of atherosclerosis,which mechanism is related to the protection of vascular endothelial function.

Objective To investigate the protective effects of recombinant human tumor necrosis factor receptor:Fc fusion protein (rhuTNFR:Ice)on the acute renal injury induced by lipepelysaccharide(LPS)in rats.Methods Models ofacute renal injury in rats were constructed by intravenous injection of LPS 10 m/kg.Forty-eight SD rats weighing 180～240g were randomly divided into 4 groups with 12 animals each group,including control group,rhuTNFR:Fc group,LPS groupand rhuTNFR:Fc+LPS group.Mean arterial pressure(MAP)wKs continuously monitored for 6 h.The levels of blood tLrea nitrogen(BUN)。Creatinine(Cr),TNF-αas well as TNF-α bioactivity were assessed.The myeloperoxinse(MPO)and superoxide dismutase(SOD)activity,content ofmalondialdehyde(MDA)were also measured.Pathologic changes of lung tissue in each group were observed by HE staining.Results Compared with LPS group,the status of hypotension and pathological manifestation in kidneys were ameliorated,and MPO activity significantly decreased in rhuTNFR:Fc+LPS group(P<0.05).Conclusion These data suggest that rhuTNFR:Fc can ablate the rise in serum TNF-α bioactivity that occurs in response to LPS,and rhuTNFR:Fc could in part protect rats from the acute renal injury induced by LPS.

Objective To investigate the expressions of indoleamine 2,3-dioxygenase (IDO) and transcription factor forkhead box P3(FOXP3)in gallbladder adenocarcinoma; and to evaluate the relationship between the expressions of IDO and FOXP3 protein,and clinicopathology and lymph node metastasis of gallbladder carcinoma.Methods The expressions of IDO and FOXP3 in 51 cases of primary gallbladder cancer,90 cases of chronic cholecystitis,and 20 cases of normal gallbladder tissues were studied by immunohistochemical staining. Results The positive expression rates of IDO and FOXP3 in gallbladder carcinoma were 72.5％ (37/51) and 60.8％ (31/51),in normal gallbladder mucosa were 5％ (1/20) and 20.0％ (4/20),in cholecystitis and gallstone were 11.1％ (10/90) and 32.2％ (29/90),respectively.There were significant differences among the three groups in IDO and FOXP3 expressions (P＜0.01).The positive expression rates of IDO and FOXP3 were 7.1％ and 14.3％ for simple hyperplasia,17.7％ and 35.3％ for atypical hyperplasia,40％ and 35％ for gallbladder carcinoma (stages Ⅰ-Ⅲ),and 77.4％ and 64.5％ for gallbladder carcinoma (stages Ⅳ-Ⅴ ).There were significant differences among the four groups in IDO and FOXP3 expressions (P＜0.01).There were significant differences among the Nevin stage groups,lymph node metastasis group and gallbladder stone in IDO and FOXP3 expressions.However,there were no significant differences among the sex groups and the age groups in IDO and FOXP3 expressions (P＞0.05).In gallbladder carcinoma,the expression of IDO had a positive correlation with FOXP3 expression in lymphocyte (γ=0.406,P=0.003).Conclusion In gallbladder cancer,a high-expression of IDO and an expression of FOXP3 in lymphocyte are closely related with immune tolerance of gallbladder carcinoma.The results provide a reference for clinical use of immunotherapy in gallbladder cancer.