Eucalyptus globulus can pbviously reduce edematous swelling of ear,inhibit the proliferation of granuloma,and blood capillary permeability of mice. It can also obviously inhibit cotton pellet granuloma implanted inrats. It has obvious anti-inflammatory and analgesic effect on arthritis in rat induced by carrageenan and painreaction in mice induced by Woolfe-Macdonald as well as HAc-induced writhing.

ObjectiveTo observe the effects and safety of combination of Ozagrel and low molecular weight heparin and rehabilitation training in the treatment of progressive cerebral infraction. Methods60 patients with progressive cerebral infraction were divided into the observation group and the control group with 30 patients in each group. 80 mg Ozagrel plus 250 ml normal saline infusion were used in the observation group, once every 12 hours, 14 days for a course of treatment, 0-4 ml low molecular weight heparin was used with subcutaneous injection every 12 hours interval time, 14 days for a course of treatment. But only Ozagrel was used in the control group. The same treatment was used in the two groups, all patients received routine rehabilitation training. Platelet and activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (Fg) were examined before and 14 days after treatment. The NIHSS score was assessed.ResultsAfter treatment, NIHSS score in both groups decreased significantly(P<0-01) while the observation group was significantly better than the control group (P<0-01). The efficiency of the observation group was 86-7%, the control group was 60-0%, the observation group was significantly higher than the control group(P<0-05). Platelet count, PT, APTT, and Fg of the two groups were in the normal range, and there was no obvious adverse reaction.ConclusionOzagrel and low molecular weight heparin and rehabilitation training is safe and effective in the treatment of progressive cerebral infraction.

Objective To observe the effect of continual rehabilitation on patients with brainstem infarction. Methods 52 stroke patientswith brainstem infarction were divided into early rehabilitation group (n=26) and continual rehabilitation group (n=26). 2 groups receivedearly rehabilitation for 1 month. Then the continual rehabilitation group continued rehabilitation in the rehabilitation unit, while the early rehabilitationgroup just received the medical guide after discharge. Fugl-Meyer Assessment (FMA), the Balance subscale of the Fugl-Meyertest (FM-B), and modified Barthel Index (MBI) were applied to assess the motor function, balance function and the activities of daily living(ADL) in two groups respectively before and 1 month and 3 months after training. Results The motor function, balance function, the performanceof activities of daily living improved in the continual rehabilitation group (P<0.05). Compared with the early rehabilitation group, thecontinual rehabilitation group significantly improved in FMA, FMA-B and MBI 3 months after the rehabilitation (P<0.05). There was a positivecorrelation in the scores among FMA and FMA-B and MBI. Conclusion The continual rehabilitation is benefit to the motor functionand balance function and ADL of the patients with brainstem infarction.

Objective To observe the effect of rosiglitazone on serum NOS/NO in apolipoprotein(apo) E knockout mice.Methods Twenty eight-week-old apoE knockout mice were intragastrically administrated 0.2 ml 5% sodium carboxymethycellulose for 12 weeks to establish the animal models of atherosclerosis,in which half of mice simultaneously received 10 mg?kg~(-1)?d~(-1) rosiglitazone as treatment.Ten wildtype mice were used as the normal control group.All mice were fed on normal chow diet.After 12 weeks,aorta were used for histomorphometric analysis by means of HE.Vessel blood was collected for plasma lipid,NO and NOS.Results Histomorphometric analysis showed that the area of atherosclerosis plaque in mice receiving rosiglitazone was significantly smaller than the mouse models of atherosclerosis,while the plasm lipid,NO and NOS were higher.Conclusion Rosiglitazone can inhibit the development of atherosclerosis,which mechanism is related to the protection of vascular endothelial function.

Objective To evaluate the value of bispectral index(BIS)monitoring as an indicator for extubation sedation level after children's oper?ation by total intravenous anesthesia. Methods One hundred and eighty children(2?13 years old)were randomly divided into six groups with 30 cases in each. Group A kept BIS 56?60,and Group B 61?65,Group C 66?70,Group D 71?75,Group E 76?80,Group F 81?85 till extubation. All children were given fentanyl,propofol and cisatracurium besilate when induced,and maintained with propofol and remifentanil. All cases kept BIS 40?55 during the operation. Then the changes of electrocardiogram,mean arterial pressure,heart rate,SpO2,postoperative complications and recovery time were observed. Results Groups A and B were extubated at deep anesthesia,cycle stability,but with high incidence of adverse reactions and awaked with a long time. Groups E and F were extubated when awaked,but with greater stimulation and easier agitation. Groups C and D were lighter hemodynamic responses,less respiratory effects and less postoperative complications of anesthesia. Conclusion The BIS level of 66?75 is a good se?dation level for extubation,especially in the level of 71?75.

Objective To study the optimal operation procedure for elderly patients with cholelithiasis. Methods 1395 elderly patients with cholelithiasis,who were more than 65 years old,were treated with operation.1356 cases of them were treated with mini-cholecystectomy(MC). Results MC were performed on 1356 patients successfully(97.2%).The incidence of the operative complication was 2.58%(35/1356).No death occurred.The mean hospital stay was 6.2 days. Conclusions Elderly patients with cholelithiasis must be operated on as early as Possible.Because of minimal invasion and quicker recovery after operation,MC will be the first choice for the treatment of elderly patients with cholelithiasis.

OBJECTIVE:To investigate the inhibitory effect of astragalus polysaccharide(APS)on the proliferation of human neuroblastoma SH-SY5Y cells. METHODS:After the cells were cultured with 0(blank control),25,50 and 100 mg/ml APS for 6,12 and 24 h,MTT method was used to determine cell viability and calculate inhibition rate. Following cell cultured with 0 (blank control),25,50 and 100 mg/ml APS for 24 h,Hoechst 33258 fluorescent staining was performed,and then cell nucleus morphology was observed under the fluorescence microscope;flow cytometer was used to detect the distribution of cell cycles and apoptosis;western blot was employed to determine the expression of extracellular regulated protein kinases (ERK) 1/2 protein in cells. Enzyme linked immunosorbent assay (ELISA) was conducted to determine the contents of interleukin 2 (IL-2),IL-6 and IL-12 in the cells. RESULTS:Compared to the blank control,those cultured with 100 mg/ml APS for 6 h,50 and 100 mg/ml APS for 12 h and 25,50 and 100 mg/ml APS for 24 h demonstrated higher inhibition rate. After the cells were cultured with 50 and 100 mg/ml APS for 24 h,those in G0/G1 phase increased and those in G2/M and S phases decreased,and the contents of IL-2 and IL-6 increased. After cells were cultured with 25,50 and 100 mg/ml APS for 24 h,the apoptosis rate was higher,densely hyperchromat-ic fragments in cell nuclei and apoptotic bodies appeared,the phosphorylation level of ERK1/2 protein in the cells was lower,and the content of IL-12 was higher. There was statistically significance (P<0.01 or P<0.05). CONCLUSIONS:APS can inhibit the proliferation of SH-SY5Y cells by arresting cell cycle and inducing cell apoptosis through a mechanism which may be correlated to the decrease in the phosphorylation of ERK1/2 and increase in cytokine.

Primary carcinoma of the gallbladder (PCG)is the common malignant tumor in the biliary system.Because there is no specific clinical manifestation, patients diagnosed with PCG are mostlyin intermediate or advanced stages.Therefore, they lose the chance of radical resection of gallbladder carcinoma and have a poor prognosis.Radiotherapy and chemotherapy are not effective palliative measures.Immunotherapy has become an alternative strategy besides operation, chemotherapy and radiotherapy.Treatment with DCs vaccines, as a new method of immunotherapy, has become more and more important.Here we review treatment with DCs vaccines in primary carcinoma of gallbladder.

ObjectiveTo observe the effect of Qishen Tongluo Zengzhi decoction on cognitive impairment in patients with acute phase of ischemic stroke.Methods A prospective randomized controlled trial was conducted, and 130 patients with acute phase of ischemic stroke and cognitive impairment accompanied by Qi deficiency and blood stasis and stagnationof phlegm-dampness syndrome admitted into the Neurology and Rehabilitation Departments of Rizhao Hospital of Traditional Chinese Medicine(TCM) Affiliated to Shandong University of TCM were randomly divided into treatment group and control group, 65 cases in each group. In the two groups, conventional internal treatment was given to all patients, and in the treatment group, additionally the Qishen Tongluo Zengzhi decoction was administered orally(composition: astragalus membranaceus 30 g, radix pseudostellariae 30 g, notoginseng 10 g,spatholobus stem 25 g, hirudo 3 g, pberetima 10 g, radix paeoniae rubra 12 g, Chinese angelica 12 g, peach kernel 10 g, carthamus tinctorious 10 g, achyranthes 12 g, radix rhapontici 10 g, rhizoma alismatis 6 g, Acorus gramineus Soland 9 g, polygala root 9 g, rhizoma cyperi 10 g, herba siegesbeckiae 15 g),one dose a day. While in the control group, oxiracetam 4.0 g intravenous drip was given, once a day. The whole course was 21 days in both groups. Before and after treatment, the cognitive function of all the patients in two groups was assessed by Mini-Mental State Examination(MMSE)and Montreal Cognitive Assessment(MoCA) scores, and incubation period and amplitude of P300 wave were recorded.Results Finally 62 cases were in treatment group and 63 cases in control group. Before treatment, the comparisons of the MMSE score, MoCA score and P300 latent period and amplitude between the two groups had no statistically significant differences(allP>0.05). After treatment in the two groups, the MMSE score, MoCA score and P300 wave amplitude were elevated, P300 latency period was shortened compared with those before treatment, and the changes were more prominent in treatment group〔score of MMSE: 25.33±2.32 vs. 21.68±2.29, score of MoCA(score): 26.61±3.06 vs. 22.40±2.93, P300 wave incubation period(ms): 349.62±20.01 vs. 371.87±19.63, P300 wave amplitude(μV): 8.70±2.92 vs. 5.72±2.33,allP<0.01〕.ConclusionQishen Tongluo Zengzhi decoctioncan effectively intervene cognitive impairment in patients with acute phase of ischemic stroke, and improve their cognitive function.

Objective To investigate the protective effects and the undedying mechanism of recombinant human tumor necrosis factor receptor: Fc fusion protein (Yisaipu, rhu TNFR: Fc) on the lipopolysaccharide (LPS) induced acute liver injury of rats. Method Totally48 SD rats were randondy divided into four groups , in-cluding control gronp (n = 12), Yisaipu group(n = 12), LPS gronp(n = 12) and Yisaipu + IPS group(n = 12). The models of acute liver injury were produced by injection of LPS intravenously. Being fasted for 12 h, the rats were anaesthetized (60 mg/kg pentobarbital sodium, i.p.) and cannulated into carotid arteries. The cannula was connected with the multi-channel creature signal analysis system. The rata in control group and LPS group were injected with normal saline or LPS in dose of 5 mg/kg through rats' sublingual vein respectively. While the rats in Yisaipu group and Yisaipu + LPS group was pretreated with Yisaipu in dose of 0.4 mg/kg subcutaneously 24 h be-fore normal saline or LPS infusion. Six rats of each goup were randomly selected and mean arterial pressure (MAP) were monitored for 6 h via multi-channel creature signal analysis system, and rats' survival rate was calcu-lated. The rats whose MAP less than 10 mmHg were considered to die and the alive rats during period of observa-tion sacrificed by exsanguination. The liver tissue at the same site was removed, fixing in 10% formalin or stored at -80 ℃. To detect serum TNF-α, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) level, 0.2 mL blood samples were collected from the carotid artery 2 and 3 h after the injection of saline or LPS. The serum was collected from centrifuged blood samples and stored at -80 ℃. Enzyme-linked immunosorbent assay (ELISA) and flow cytometry were used to assess serum TNF-α level and bioactivity respectively. We also measured the serum ALT and AST levels, the myeloperoxiase (MPO) and superexide dismutase (SOD) activity, and malon-dialdehyde (MDA) content in liver tissue The pathology of hepatic tissue was evaluated by HE staining. Statistical-ly,the data of TNF-α level and bioactivity, ALT and AST release, and MDA content were analyzed by ANOVA, and rat survival rate were analyzed by Chi-square Tests. Results The rats in control group and Yisaipu group were all survived. Rat survival rate was significantly higher in Yisaipu + LPS group (67%) than in LPS group (17%) (P < 0.05). Serum TNF-α bioactivity was significantly lower in Yisaipu + LPS group than in LPS group [(7.3±2.8)% vs.(51.3±6.4)%, P <0.05]. Compared with IPS group, Yisaipu pretreatment decreased MDA content [(1.40±0.10)vs. (2.81±0.11) nmol/mgprot, P <0.05]and MPO acticity [(0.38±0.04) vs. (0.54±0.02) U/g, P <0.05]in hepatic tissue, while SOD activity [(188.4±20.2) vs. (142.5 ± 18.3) U/mgprot, P <0.05]was increased. The serum AST level, ALT level and the pathology in the liver were also ameliorated correspondingly. Conclusions These data suggest that Yisaipu could protect rats from LPsinduced a-cute liver injury by inhibiting TNF-α bioactivity and by enhancing anti-oxidation.