The laboratory diagnosis of peripheral blood cell morphology by manual light microscopy remains one of the major labor-intensive procedures at the present time.The development speed and clinical application of blood cell morphology have been restricted evidently as a result of the manual procedure and so on.A revolutionary change appears in the laboratory diagnosis of peripheral blood cell morphology because automated digital image analysis (ADIA) has grown rapidly in the last ten years.A development trend will be the laboratory diagnosis of peripheral blood cell morphology by ADIA in future,and which may replace a majority of morphologic examination by manual microscopy in the wake of the technique use in medical laboratory.

A number of common view from Chinese experts with regard to diagnosis and treatment of hemorrhagic and thrombotic diseases had been recently released by Chinese Medical Association,in which is of importance about some items and its clinical application of thrombosis and hemostasis tests which have a significant effect on the decision-making of clinical diagnosis and treatment.It will be discussed that diagnostic standard,the key to the question and strategies of solution which based the common view in laboratory diagnosis for several kinds of hemorrhagic and thrombotic diseases,and it is very useful to the experts working on laboratory diagnosis of hemorrhagic and thrombotic diseases.

In this paper the followings are reviewed; 1) seal oil and it's processing method, fatty acids constitutents, quality standards, applications in food, pharmaceutical and cosmetic. 2) general components of seal meat, extraction of hemin, physiological functions of peptides and vasoactive substances in seal meat. 3) preparation and applications of male reproductive organs of seal as traditional Chinese medicine having invigorating Yang efficacy.

Flow cytometric immunophenotyping (FCI) of hematolymphoid neoplasia is a high-complexity assay with proper medical indications,and it requires a hish-standardization in the choice of antibody panel,analysis and reports of the data.It is necessary to provide a specialized training and education for laboratory professionals who perform FCI for hematolymphoid neoplasitr.

Many platelet function tests can be used for monitoring antiplatelet drug for the prevention and treatment of thrombotic diseases.These monitoring tests may be chosen based on different antiplatelet drugs which include aspirin,clopidogrel and GP Ⅱ b/Ⅲ a antagonist.There is difference of the drug resistance which is validated by different platelet function tests,and the relation with clinical adverse events should be farther clarifled.

Rabbits were used in examination of pulmonary coefficient, pulmonary light microscopic examination, coagulation test, blood gas and pH in hemorrhagic shock, blockade of the RES and hemorrhagic shock after blockade of the RES. Our studies indicate that the pulmonary injury was only seen in hemorragic shock after blockade of RES.The portal vein blood in hemorragic shock after the depression of RES was perfused to other rabbits of only depression of RES. Release of leukocytes from the liver and spleen, leukocytes entrapment in the lungs and pulmonary injury were observed in the perfused rabbits. The results suggest that a pathologic factor causing pulmonary injury is present in the portal vein blood of shocked rabbits after the depression of RES.

BACKGROUND:It is likely to find that ABCE1 plays an important part in the occurrence and progression of cancers through in-depth study. OBJECTIVE:To investigate the effects of ABCE1 silencing on the proliferation and sensitivity toβ-eiemene of ovarian cancer stem cel s via electrotransfection. METHODS:siRNA sequence of ABCE1 was designed and synthesized, and then was electrotransfection into ovarian cancer stem cel s. Subsequently, expressions of ABCE1 mRNA and protein in ovarian cancer stem cel s after ABCE1 silencing were detected by RT-PCR and western blot assay;the cel cycle was detected using flow cytometry;the sensitivity of ovarian cancer stem cel s toβ-eiemene after ABCE1 silencing was analyzed by MTT assay and colony-formation assay. RESULTS AND CONCLUSION:After ABCE1 silencing, expressions of ABCE1 mRNA and protein in ovarian cancer stem cel s were significantly reduced. And the cel cycle was arrested in G0/G1 phase, and the number of cel s in S phase significantly decreased. Furthermore, the sensitivity of ovarian cancer stem cel s toβ-eiemene after ABCE1 silencing was significantly enhanced. In conclusion, ABCE1 silencing cannot only significantly inhibit the proliferation of ovarian cancer stem cel s, but also enhance the sensitivity of cancer stem cel s toβ-eiemene.

Objective To investigate the clinical value of anti-nuclear antibody(ANA) and anti-nuclear antibody spectrum(ANAs) detection.Methods A total of 2 325 patients with or suspected with autoimmune diseases(AID) were enrolled and detected for ANA and ANAs by using indirect immunofluorescence assay(IIF) and linear immunoblot assay(LIA) respectively.All detected results were analyzed.Results Among 2 325 patients,896 cases(38.54%) were positive with ANA,with positive rate of 45.46% in female patients,which was higher than the 18.46% of male patients(P<0.05),and the common fluorescence patterns were nuclear particle pattern,nuclear homogeneous pattern and the nucleolus pattern.816 cases(35.10%) were positive with ANAs,and the positive rates of anti-Sjogren's syndrome(SS)-B antibody,anti Ro-52 antibody and anti SS-A antibody were relatively higher.The consistency rate of the two methods was 91.66%.Conclusion ANA and ANAs detection could be with certain correlation,but might be not completely consistent,detection could improve the detection rate and reduce the missed detection rate.

Objective To evaluate the effects of unilateral extralevator abdominal-perineal excision (ELAPE) surgery and the ELAPE surgery on the pelvic floor detected by finite element analysis. Methods Three kinds of finite element mod?el were developed:the intact model, ELAPE model and the unilateral ELAPE model. The maximal stress and stress distribu?tions of each model under the same pressure were analyzed and compared. Results In the unilateral ELAPE model, non-le?vator ani tissue’s maximal stress on the levator ani reserved side was lower than that in ELAPE model, and was similar to that in the intact model. Its maximal stress on the excised side was lower than that in ELAPE model, and which was higher than that of intact model. Its maximal stress on the reserved side was lower than that of on the excised side. Conclusion Compared to the ELAPE surgery, the unilateral ELAPE surgery is able to reduce the stress of non-levator ani tissue on both sides, especially on the levator ani reserved side.

Objective Distortion product otoaconstic emission (DPOAE) was compared withauditory brain－stem response (ABR) in the early diagnosis of gentamicin (GM) ototoxicity. Whetherimpaired hearing could be improved with the glutathione treatment after its withdrawl of injection.Methods40 guinea pigs with normal hearing were randomly divided into 4 groups, 10 in DPOAEgroup, 12 in ABR I group (the usage of GM was stopped when abnormal shift presented in ABR), 12 inABR Ⅱ (group glutathione was injected for 5 after the usage of GM had been stopped), 6 in controlgroup. The guinea pigs in experimental group were injected with GM 100mg/kg per day, while those inthe control group were injected with saline. The DPOAE and ABR were monitored to observe the ampli-tude and the threshold of wave IV. The morphological changes of hair cells were also observed after injec-tion of GM had been stopped for 2 weeks. ResultsIt was averaged at 7d after the injection of GMwhen the changes presented in DPOAE group, while 10d in ABR group. The threshold shift increasedafter the injection had been stopped for 2 weeks in ABR Ⅰ group ( P <0.01) ,while there was no obvi-ous shift in ABR Ⅱ group. The morphological and functional changes of the hair cells were almost thesame. Conclusion GM ototoxicity could be more sensitively observed by DPOAE than ABR. Glu-tathione could prevent further ototoxic effect of GM to the cochlear.